[Natural killer cells (CD56, CD16) as a factor of local antitumor immunity in breast cancer].

Two groups of malignancies with significantly distinct ratios of Ki-67 and CD16 expression were identified. Significant direct and inverse correlations between the markers were established (p < 0.01) whereas either group revealed two contradicting patterns of immune response. Whatever hormone receptor, natural killer cells (IKC) (CD16) levels correlated directly with tumor size and lymph node incidence (p < 0.01). IKC (CD56) did not contribute to breast tumor response. Our findings on CD16 expression may be used as criteria in prognosis prediction.

[Comparative evaluation of proliferative activity of breast tumors of different estrogen-progesterone receptor status].

Immunological investigation involving 100 estrogen-progesterone receptor negative (RE-RP-) and 71 estrogen-progesterone receptor positive (RE+RP+) breast cancer patients demonstrated that Ki-67 expression in the former group was twice (49.8%) that in the latter (25.9%). Low Ki-67 expression was found in 39% of RE-RP-tumors whereas high expression--in 28% of RE+RP+ tumors.

[Production of soluble form of human TNF-alpha ligand-binding domain type 1 receptor by expression in Drosophila cells].

5His-tagged human TNFalpha type I receptor (TNFR1) ligand-binding domain was produced in Drosophila cells under control of metallothionein Cu-inducible promoter and purified by Ni-NTA affinity chromatography to homogeneity. TNFR1 gene fragment was cloned by PCR from CD8+ in vitro cultured T-killer normal linage cDNA. In despite of three disulfide bonds, the recombinant protein was correctly folded which was conformed by TNFalpha ligand binding assay in ELISA variant.

[New potential of estrogen and progesterone receptor assay in cytological smears sampled from breast cancer patients]. / Vozmozhnosti opredeleniia retseptorov éstrogenov i progesterona v rake molochnoi zhelezy na tsitologicheskikh mazkakh.

Estrogen (ER) and progesterone (PR) receptors were assayed immunohistochemically in cytological smears and histological samples from 30 breast cancer patients. Since the results reported by the two research centers matched by 73%, it is suggested that running ER and PR determinations on cytological smears in cases of inoperable cancer, preoperative therapy and monitoring may be recommended. Therefore, the 3-4 year old records on ER and PR determinations should be regarded as null and void.

Cytotoxic activity of endometrial immunocompetent cells determines the outcome of embryo implantation.

The data on the formation of blastocysts in culture during therapeutic cycles of extracorporeal fertilization and on cytotoxic index of the endometrium in women with different clinical pathogenetic variants of sterility are presented. Analysis of correlations of these parameters with the incidence of effective implantation and pregnancy revealed a strict correlation between cytotoxic activity of endometrial immunocompetent cells and human embryo implantation.

[Effect of heat shock on transpositions of Mobile Genetic Element Dm412 in three isogenetic lines of Drosophila melanogaster]. / Vliianie teplovogo shoka na transpozitsii MGE Dm412 v trekh izogennykh liniiakh Drosophila melanogaster.

Induction of Dm412 transpositions by heavy heat shock was studied in several isogenic lines of Drosophila melanogaster. The probabilities of spontaneous and induced transpositions were estimated. Their respective values were less than < 3.0 x 10(-3) and 3.8 x 10(-2) per site per genome per generation in line 51, and less than < 3.6 x 10(-3) and 2.6 x 10(-2) in line 66. These estimates are close to those obtained earlier for Dm412 (less than < 3.3 x 10(-4) and 7.2 x 10(-2) and for B104, line 49 (less than < 1.7 x 10(-3) and 8.1 x 10(-2)). Thus, transposition induction of copia-like mobile genetic elements by heavy heat shock is a general phenomenon common for various isogenic lines of Drosophila. Other properties of Dm412 and B104 transpositions, such as the presence of "hot" and "cold" sites, HSI haplotypes etc., are also similar.