RESUMO
Inflammatory myofibroblastic tumor (IMT) is an extremely rare disease composed of myofibroblast cells and inflammatory infiltrates. There are different sites of the urogenital system affected by IMT-bladder, prostate and kidney. We report a case of a 59-year-old male patient presented with abdominal pain, gross hematuria and a renal mass treated with partial nephrectomy. The final diagnosis was renal inflammatory myofibroblastic tumor. Despite recent improvements in imaging technology, preoperative diagnosis of IMT remains a dilemma. It is therefore mandatory to carry out clinical interpretation, careful histologic examination, and immunohistochemical studies which will generally determine the appropriate diagnosis and patient management.
RESUMO
Electric field pulses used for cell manipulation can cause irreversible cell damage. The mechanisms of the processes leading to such cell damage are very complicated. Our work demonstrated that exponential electric pulses with intensity of 2-7.5 kV/cm and duration of 5.2 ms were able to initiate peroxidation of fatty acid emulsions, liposomal membranes, red blood and Ehrlich ascite tumor cells. Electric pulses-induced peroxidation of erythrocyte membranes was followed by hemolysis. The electric treatment caused damage of E. coli membrane lipids which was accompanied by decreased cell survival. All these effects depended on field intensity. A relatively good correlation between pulse-induced peroxidation of erythrocyte membranes and hemolysis was observed. These results suggest that free radical mediated processes as lipid peroxidation and/or lipid degradation or fragmentation may be possible causes for electric pulses-induced irreversible cell damage.
Assuntos
Eletroporação/efeitos adversos , Lipídeos de Membrana/metabolismo , Animais , Carcinoma de Ehrlich/metabolismo , Emulsões , Membrana Eritrocítica/efeitos dos fármacos , Membrana Eritrocítica/metabolismo , Escherichia coli/efeitos dos fármacos , Escherichia coli/metabolismo , Sequestradores de Radicais Livres/farmacologia , Hemólise/efeitos dos fármacos , Técnicas In Vitro , Cinética , Ácido Linoleico , Ácidos Linoleicos/química , Peroxidação de Lipídeos/efeitos dos fármacos , Lipossomos , Membranas Artificiais , Camundongos , Oxirredução , Ratos , Substâncias Reativas com Ácido Tiobarbitúrico/metabolismo , Células Tumorais Cultivadas/efeitos dos fármacos , Células Tumorais Cultivadas/metabolismoRESUMO
Currently, the major problem in the genetic transformation is to understand how such a large molecule as the plasmid DNA passes through the cell membrane. To solve this problem we used a simplified experimental model with Escherichia coli and the plasmid pBR322: the DNA-bacteria mixture was electroporated in a sucrose solution at 0 degree C and at fixed electrical parameters; the samples were then directly plated into agar. It was found that the electrically-induced bacterial transformation after pulsing is dependent on two factors: heat shock (delta T) and osmotic stress. Our results indicate that two mechanisms contribute to these effects: (i) thermodiffusion of the DNA across the membrane and (ii) osmotic flows of the medium, containing the DNA, into the interior of the cell.
Assuntos
DNA Bacteriano/metabolismo , Escherichia coli/genética , Membrana Celular/metabolismo , Difusão , Campos Eletromagnéticos , Eletroporação , Temperatura Alta , Concentração Osmolar , PlasmídeosRESUMO
Among 2,778 patients with mechanical injury, 361 (13.3%) had closed abdominal trauma. The authors distinguish two syndromes in the clinical picture: developing peritonitis and intraabdominal blood loss. Laparocentesis is conducted if injury to the viscera is suspected; the authenticity of the intervention comes up to 97.9-100%. Ultrasonic examination may be undertaken if hemodynamics are stable before laparocentesis.
Assuntos
Traumatismos Abdominais/diagnóstico , Hemoperitônio/diagnóstico , Peritonite/diagnóstico , Ferimentos não Penetrantes/diagnóstico , Traumatismos Abdominais/complicações , Traumatismos Abdominais/cirurgia , Músculos Abdominais/cirurgia , Emergências , Hemoperitônio/etiologia , Hemoperitônio/cirurgia , Humanos , Lavagem Peritoneal , Peritonite/etiologia , Peritonite/cirurgia , Ferimentos não Penetrantes/complicações , Ferimentos não Penetrantes/cirurgiaRESUMO
This communication provides evidence that the thermo-osmotically induced membrane defects and fluxes occurring in plant protoplasts may initiate internalization and fusion of adjacent cells. This phenomenon is called thermofusion of cells.
Assuntos
Fusão Celular , Temperatura Alta , Protoplastos , Agregação Celular , Campos Eletromagnéticos , Eletroforese/métodos , Manitol , Plantas Tóxicas , NicotianaRESUMO
A simple and rapid nonenzymatic method for radioiodination of phospholipids is described. It involves oxidation of Na125I with TlCl3 (or chloramine-T) in an aqueous medium, with subsequent exposure of the phospholipids, dissolved in chloroform/methanol, to the action of the oxidizing mixture. Purification of the radiolabelled phospholipids was effected by washing with sodium thiosulphate followed by thin-layer chromatography on silica gel. Specific radioactivity of 125I-labelled phosphatidylcholine was estimated to be about 10 muCi/mg phospholipid. The method is designed for radioiodination of various naturally occurring phospholipids.