RESUMO
A retrospective study was undertaken of patients with T1N0M0 squamous cell carcinoma of the oral tongue and floor of the mouth who underwent surgical treatment between 1985 and 1995. Evaluation of two groups of patients (neck dissection versus observation) was made according to the management of the neck. Results were obtained regarding the presence of occult metastases, recurrence in the neck, treatment failure, results of salvage treatment, and disease-free survival. Forty-nine patients underwent surgical treatment: 25 resection of primary and 24 resection plus neck dissection. Overall incidence of regional metastases was 24.5%. Eight patients (16%) developed recurrence of the disease. Seven (14%) had regional recurrences (including 1 with distant metastases) and 1(2%) had local recurrence. Twenty-four percent of patients from the resection of primary group developed neck recurrences in comparison with 4% of the resection plus neck dissection group (P = 0.05). Overall salvage rate was 37.5%. Second primary tumors developed in 16% of patients. Patients who underwent elective neck dissection had a 23% higher disease-free survival rate compared with those who underwent resection of the tumor alone (P = 0.03). The findings of this study stress the importance of control of the neck in early oral cancer. Elective neck dissection significantly improved regional control of the disease.
Assuntos
Carcinoma de Células Escamosas/cirurgia , Neoplasias Bucais/cirurgia , Recidiva Local de Neoplasia/terapia , Terapia de Salvação , Neoplasias da Língua/cirurgia , Adulto , Idoso , Carcinoma de Células Escamosas/mortalidade , Carcinoma de Células Escamosas/patologia , Intervalo Livre de Doença , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Soalho Bucal/patologia , Neoplasias Bucais/mortalidade , Neoplasias Bucais/patologia , Recidiva Local de Neoplasia/mortalidade , Recidiva Local de Neoplasia/patologia , Estadiamento de Neoplasias , Observação , Probabilidade , Prognóstico , Estudos Retrospectivos , Estatísticas não Paramétricas , Taxa de Sobrevida , Neoplasias da Língua/mortalidade , Neoplasias da Língua/patologia , Resultado do TratamentoRESUMO
Hypoxanthine-phosphoribosyltransferase negative (Hprt-) cell lines derived from an Akodon cursor liposarcoma were obtained by induced mutagenesis. All but one Hprt- cell line lacked Hprt mRNA transcripts while one (AKO 3) coded for a truncated protein. Cell fusion and karyotypic analyses showed that one cell line (AKO 1-15) could be successfully used for constructing hybrid panels and allow for a clear identification of human chromosomes in hybrid cells.
Assuntos
Cromossomos/genética , Células Híbridas/citologia , Células Híbridas/enzimologia , Hipoxantina Fosforribosiltransferase/deficiência , Muridae/genética , Mutagênese/genética , Aneuploidia , Animais , Fusão Celular , Humanos , Células Híbridas/metabolismo , Hipoxantina Fosforribosiltransferase/química , Hipoxantina Fosforribosiltransferase/genética , Cariotipagem , Lipossarcoma/enzimologia , Lipossarcoma/genética , Lipossarcoma/patologia , Linfócitos/citologia , Linfócitos/metabolismo , RNA Mensageiro/análise , RNA Mensageiro/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Deleção de Sequência/genética , Células Tumorais CultivadasRESUMO
The culturing of gerbil epidermal cells is described for the first time. Cells grew first as clumps, formed monolayers of proliferating basal cells and then differentiated, giving rise to a stratified epithelium. To regulate the process of proliferation and differentiation, two kinds of medium were used: the standard one and a low-Ca++ medium. Under these two conditions, cultured gerbil cells were compared with mouse epidermal cell cultures. The cultures maintained in the low-Ca++ medium showed greater differences between the two species, the gerbil epidermal cells displaying a greater proliferative capacity and maintained capacity to differentiate, than did the murine cells. It is believed that this in vitro model may lead to a better understanding of the difference between the two species in vivo, and might explain their differing susceptibility to cutaneous tumour induction.
Assuntos
Células Epidérmicas , Gerbillinae/fisiologia , Animais , Cálcio/fisiologia , Divisão Celular , Células Cultivadas , Meios de Cultura , Feminino , Masculino , Camundongos , Especificidade da EspécieRESUMO
The effects of TPA (12-0-tetradecanoylphorbol-13-acetate) and RA (retinoic acid) were investigated on the cell lines HL60 (acute promyelocytic leukemia) and K562 (erythroleukemia) and on cells from patients with several kinds of leukemia. There were 14 cases of acute lymphocytic leukemia (ALL), 2 cases of chronic lymphocytic leukemia (CLL), 23 cases of acute myeloid leukemia (M1-M7), 5 cases of chronic myelocytic leukemia in blast crisis (CML-BC) and 2 mixed leukemias. In almost all of the cases examined, after TPA exposure cells from patients with proven myeloid leukemia became adherent to the substrate, while lymphoid leukemia cells remained in suspension, allowing the differentiation of lymphoid from myeloid blasts. The only exception was in one case of CLL, which had cells that became adherent with long filamental projections. In addition, increased phagocytosis following TPA exposure permitted characterization of M7 as this was the only myeloid leukemia negative for phagocytosis. Further discrimination between the subtypes of myeloid leukemia could be based on the increased lysozyme production seen after TPA in M4 and M5. Esterase positivity allowed the discrimination of M1 cells, which were negative before and after TPA treatment. In agreement with the results of other authors, TPA and RA led to independent ways of differentiation, granulocytic-like lineage and monocytic-like cells being favored by RA and TPA, respectively. The capacity of the same cell to differentiate into more than one lineage, depending on whether RA or TPA was used, was only seen in the present study with M3 cells.(ABSTRACT TRUNCATED AT 250 WORDS)