Assuntos
Osteomielite , Infecções Estafilocócicas , Humanos , Vértebras Torácicas/diagnóstico por imagem , Antibacterianos/uso terapêutico , Infecções Estafilocócicas/tratamento farmacológico , Osteomielite/diagnóstico , Osteomielite/tratamento farmacológico , Imageamento por Ressonância Magnética , Vértebras Lombares/diagnóstico por imagemAssuntos
Doenças Autoimunes/diagnóstico , Edema/etiologia , Imunoglobulina A/sangue , Dor Lombar/etiologia , Vasculite/diagnóstico , Doenças Autoimunes/complicações , Doenças Autoimunes/imunologia , Biomarcadores/sangue , Criança , Feminino , Humanos , Região Lombossacral , Vasculite/complicações , Vasculite/imunologiaRESUMO
O-phospho-l-serine sulfhydrylase (OPSS) from archaeon Aeropyrum pernix K1 is able to synthesize l-cysteine even at 80 °C. In this article, we compared thermal stability and reactivity in organic solvent of OPSS with those of O-acetyl-l-serine sulfhydrylase B (OASS-B) from Escherichia coli. As a result, the thermostability of OPSS was much higher than that of OASS-B. Moreover, the activity of OPSS increased in the reaction mixture containing the organic solvent, such as N, N'-dimethyl formamide and 1,4-dioxane, whereas that of OASS-B gradually decreased as the content of organic solvent increased. From the crystal structural analysis, the intramolecular electrostatic interactions of N-terminal domain in OPSS seemed to be correlated with the tolerance of OPSS to high temperature and organic solvent. These results indicate that OPSS is more superior to OASS-B for the industrial production of l-cysteine and unnatural amino acids that are useful pharmaceuticals in the presence of organic solvent.
