[Sergei G. Kryzhanovsky (1891-1961)].

This essay about the outstanding Russian embryologist and ichthyologist is not time to any date of his life. The cause for its appearance was an unexpected meeting with Elena N. Disler, whom I did not see almost 60 years. When I worked at the Biological Station "Glubokoe ozero" (Deep Lake), there were also biologists N.N. Disler and his wife E.F. Eremeeva with their children Lena and Vitya. At the same time, as usual, S.G. Kryzhanovsky worked at the station, who lived in Moscow in the same apartment with the Dislers. After the death of this lone scientists, his archive, mostly epistolary, was in the care of the Dislers. And Elena N. Disler (that girl Lena) proposed for me to have a look at this archive.

[Stathmokinetic reaction reversibility in malignantly transformed cells]. / Obratimost' statmokineticheskoi reaktsii v zlokachestvenno transformirovannykh kletkakh.

The statmokinetic reaction of malignant-transformed cells of a hamster tumor (CHT-1) was induced by colcemid. The reversibility of the reaction is primarily related to the repolymerization of spindle microtubules from the tubuline pool of the precursors. The character of the statmokinetic reaction reversibility in the test cells evidences the activity of polymerization processes in them. The course of these processes in the presence of copper ions did not differ from that in nonmalignant-transformed cells.

[Pathology of mitosis after cellular recovery from a metaphase block]. / Patologiia mitoza posle vykhoda kletok iz metafaznogo bloka.

The experiments in Chinese hamster fibroblast-like cells have shown that the c-mitosis inducing agents (colchicine, colcemid and low temperature) produce evident stathmokinetic and radiomimetic effects. By the reversibility moment of the former the latter becomes apparent, manifesting in bridge accumulation. This form of pathology is likely to be caused by the disturbance of the cellular nucleoprotein metabolism in c-mitosis.

[Mechanism of reversibility of colchicine mitosis induced by colcemid]. / Mekhanizm obratimosti kolkhitsinovogo mitoza, indutsirovannogo koltsemidom

Restoration of the normal mitosis after its block by colchicine was due to the additional protein synthesis (reformation of the spindle microtubules). Studies of reversibility of the statmokinetic reaction induced by colcemid showed the block of the protein synthesis by puromycin to have no influence on the rate of restoration of the normal mitotic regimen, but copper ions delayed this process. On the basis of these results it is considered that restoration of the K-mitosis induced by colcemid was mainly due to the tubule reassembly. Probably the prevalence of the processes of reformation and reassembly of microtubules during the restoration depended on the extent of their destruction and the pattern of binding by the damaging agent of the tubular protein pool subunits.