RESUMO
The co-existence of metal oxide nanoparticles (MONPs), carbon-based nanomaterials and microplastics (MPs) in the natural environment are expected to be of growing global concern due to their increasing abundance and persistence in the environment worldwide. Knowledge of the interaction of the above compounds particularly under light irradiation in water remains limited. In the present study, the possible individual and combined toxic effects of MONPs, carbon nanotubes (CNTs) through styrofoam (SF) on the environmental bacterium Jeotaglicoccus huakuii were systematically investigated. The fabricated MONPs and CNTs were characterized using the following techniques: FT-IR (functional groups), XRD (crystallinity), SEM, and EDX (topological morphology). The objective of this study was to investigate and identify naturally occurring bacteria capable of mitigating and detoxifying toxic pollutants under adverse conditions. Moreover, the assessment of minimum inhibitory concentration (MIC) was made through an agar well plate method, resazurin (ELISA measurement), growth kinetics and bacterial viability were assessed employing live and dead assay and biofilm combating ability was analyzed using an antibiofilm assay. Further, the biotransformation of f-MWCNTs by J. huakuii was evaluated employing RT-PCR and SEM analysis. The results demonstrated that the toxicity of Pb3O4@f-MWCNTs was comparatively higher than the remaining Pb3O4 NPs and SF coated NPs.. Interestingly, J. huakuii showed resistance against f-MWCNTs at very high concentrations and able to utilize f-MWCNTs as a sole carbon source suggesting J. huakuii as a suitable aquatic bioremediation tool for both MONPs and CNTs transfer via MPs. The results also enhanced our understanding of the affinity of MPs towards MONPs and CNTs under extreme environmental conditions.
RESUMO
Developing a polymer-based photocrosslinked 3D printable scaffolds comprised of gelatin methacryloyl (G) and hyaluronic acid methacryloyl (H) incorporated with two molecular weights of polyethylene glycol diacrylate (P) of various concentrations that enables rabbit adipose-derived stem cells (rADSCs) to survive, grow, and differentiate into smooth muscle cells (SMCs). Then, the chemical modification and physicochemical properties of the PGH bioinks were evaluated. The cell viability was assessed via MTT, CCK-8 assay and visualized employing Live/Dead assay. In addition, the morphology and nucleus count of differentiated SMCs were investigated by adopting TRAP (tartrate-resistant acid phosphatase) staining, and quantitative RT-PCR analysis was applied to detect gene expression using two different SMC-specific gene markers α-SMA and SM-MHC. The SMC-specific protein markers namely α-SMA and SM-MHC were applied to investigate SMC differentiation ability by implementing Immunocytofluorescence staining (ICC) and western blotting. Moreover, the disk, square, and tubular cellular models of PGH7 (GelMA/HAMA=2/1) + PEGDA-8000 Da, 3% w/v) hybrid bioink were printed using an extrusion bioprinting and cell viability of rADSCs was also analysed within 3D printed square construct practising Live/Dead assay. The results elicited the overall viability of SMCs, conserving its phenotype in biocompatible PGH7 hybrid bioink revealing its great potential to regenerate SMCs associated organs repair.
