Histopathological, morphological, and molecular characterization of fish-borne zoonotic parasite Eustrongylides Excisus infecting Northern pike (Esox lucius) in Iran.

Eustrongylides excisus is a fish-borne zoonotic parasite known to infect various fish species, including Northern pike (Esox Lucius). This nematode, belonging to the family Dioctophymatidae, has a complex life cycle involving multiple hosts. This study aimed to investigate the occurrence of Eustrongylides nematodes in Northern pike (E. Lucius) collected from Mijran Dam (Ramsar, Iran). Between June and October 2023, an investigation was conducted on Northern pike from Mijran Dam in Ramsar, Iran, following reports of reddish parasites in their muscle tissues. Sixty fish were examined at the University of Tehran, revealing live parasites in the muscles, which were then analyzed microscopically and preserved for a multidisciplinary study. The skeletal muscle tissues of 85% (51/60) of fish specimens were infected by grossly visible larvae which were microscopically identified as Eustrongylides spp. In histopathological examination, the lesion was composed of encapsulated parasitic granulomatous myositis. Microscopically, the cystic parasitic granulomas compressed the adjacent muscle fibers, leading to their atrophy and Zenker's necrosis. Moreover, epithelioid macrophages, giant cells and mononuclear inflammatory cells were present around the larvae and between the muscle fibers. Finally, a molecular analysis by examining the ITS gene region, revealed that they belong to the species E. excisus. Eustrongylidiasis in northern Iran necessitates further research into the biology, epidemiology, and control of Eustrongylides nematodes, focusing on various hosts. This study is the first to comprehensively characterize E. excisus in Northern pike in Ramsar, Iran, raising concerns about possible zoonotic transmission.

Evaluation of close up antimicrobial therapies for treatment and prevention of subclinical mastitis in the herds with high prevalence of Staphylococcus aureus.

This field trial was conducted to evaluate two antibiotics at a close-up period in curing the existing IMI and to prevent new clinical and subclinical Intramammary infection (IMI). Two hundred and twelve Holstein cows were assigned to one of three treatment groups: TYLO, MARB and CONT. Cows in TYLO group received 10 mg/kg Tylosin for three days at the close-up period (21 days before calving), cows in MARB group received single SC injection of 8 mg/kg SC marbofloxacin at the close-up period and cows in CONT group remained untreated. Milk samples were collected for somatic cell count, microbial culture and Total oxidant/antioxidant capacity before drying-off, and 3 and 7days post calving. Antimicrobial susceptibility test and RAPT-PCR were performed on S.aureus isolates. No significant differences were detected in total cure rate within the groups, but S. aureus cure rates in TYLO and MARB were significantly higher than in CONT (74 and 73.5 % Vs 58.1 %). No significant differences in total new IMI were observed. Furthermore, the rate of new S. aureus IMI was higher in both treatment groups than in CONT group. Clinical mastitis rate in TYLO (3.8 %) and MARB (5.8 %) was significantly lower than CONT (11.3 %). Paired S. aureus isolates from dry-off and post-calving have been clustered into 9 different RAPD types (A-I). 8 paired strains collected at dry-off were identical to those at post-calving,and 35 strains had more than 60 % dissimilarity. Administration of Tylosin or Marbofloxacin is not useful in all cases;however, they have the potential to reduce the incidence of post-calving clinical mastitis and improve S.aureus cure rate if used selectively.

Radiographic assessment of normal parameters of forelimb distal phalanx in Turkmen horses.

The Turkmen horse is one of the oldest breeds in the world with unique characteristics in agility, endurance and jump, being publicly acclaimed. Laminitis affects the limbs and decreases athletic performance. Radiographic evaluation is essential to confirm the sinking diagnosis in every breed of a horse suffering from laminitis. Since no information about distal phalanx radiographic measurements in this breed has been reported, the current study was designed and conducted. In the present study, 24 clinically sound registered Turkmen horses of both sexes (15 mares and nine stallions) were selected. Lateromedial radiographic views of both front distal phalanges were taken, focusing on the distal phalanx. After magnification correction, images were used to measure founder distances (FD) and other distal phalanx radiographic measurements using an image processing program. There was no significant difference between radiographic measurements of the left and right front feet. The FD had a significant positive correlation with weight. The horses under 3 years of age had a significantly greater U angle than the horses over the age of three. Also, palmarocortical length (PCL) in the Turkmen stallions was significantly higher than mares. In this investigation, PCL in horses under the age of three was significantly lower than horses over 3 years of age. The values determined in this study can be used as baseline data of front feet in Turkmen horses.

Feline dermatoses at Ferdowsi University of Mashhad (Iran): 154 cases (2009-2020).

BACKGROUND: Studies indicate that 6% to 20% of feline patients have at least one dermatopathy. HYPOTHESIS/OBJECTIVE: This retrospective study aimed to provide comprehensive information about the types of skin diseases, diagnosis methods, treatments and follow-up data in a hospital population of domestic cats in Iran. ANIMALS/MATERIALS AND METHODS: The present survey of clinical observations was conducted on the medical records of 1825 individual cats brought to the Faculty of Veterinary Medicine, Ferdowsi University of Mashhad (Iran) over a 12-year period (2009-2020). RESULTS: Feline dermatoses accounted for 154 (8.43%) of the 1,825 cats examined during the study period. The most prevalent dermatological manifestations in the studied population of 154 were erythema, macules, papules and/or pustules (36; 23.37%), alopecia (18; 11.68%) and erosive/ulcerative lesions (17; 11.03%). Specific dermatological diagnoses made were the following (in descending order, of 154): dermatophytosis (34; 22.07%), subcutaneous abscesses (16; 10.38%), atopic skin syndrome/environmental allergies (20; 10.38%), external parasites (15; 9.74%), otitis (10; 6.49%), flea infestation (6; 3.89%), food allergy (4; 2.59%) and primary pyoderma (2; 1.29%). Most lesions were observed in the head/face of cats (41 of 154; 26.62%) and their ears (39; 25.32%). Following treatment, 86 of 154 cats (69.35%) had complete remissions, 17 cats faced recurrent dermatoses and the owners of 21 cats were not satisfied. CONCLUSIONS AND CLINICAL RELEVANCE: The prevalence of dermatoses among this cohort of Iranian cats is similar to that in other parts of the world. However, a noticeable difference was detected in the most prevalent dermatoses. To the best of our knowledge, this is the first study to assess the characteristics and outcomes of feline dermatological conditions in Iran.

Les dossiers médicaux de 1825 chats consultés à la Faculté de médecine vétérinaire de l'Université Ferdowsi de Mashhad, Iran, sur une période de 12 ans (2009­2020) ont été analysés rétrospectivement et les dermatoses représentaient 154 (8.43%) cas. La dermatophytose (34; 22.07%), les abcès sous­cutanés (16; 10.38%) et le syndrome cutané atopique (20; 10.38%) ont été le plus souvent diagnostiqués, de façon similaire à d'autres localisations géographiques; l'ordre des diagnostics les plus fréquents différait.

Os prontuários médicos de 1825 gatos que foram consultados na Faculty of Veterinary Medicine de Mashhad, Irã por um período superior a 12 anos (2009­2020) foram analisados e as dermatoses correspondiam a 154 (8.43%) dos casos. A dermatofitose (34; 22.07%), abscessos subcutâneos (16; 10.38%) e síndrome atópica cutânea (20, 10:10.38) foram as doenças mais comumente, corroborando com outros relatos de locais distintos; a ordem dos diagnósticos frequentes diferiu.

Se analizaron retrospectivamente los historiales médicos de 1825 gatos llevados a la Facultad de Medicina Veterinaria de la Universidad Ferdowsi de Mashhad, Irán, durante un período de 12 años (2009­2020), y las dermatosis representaron 154 (8.43%). Los diagnósticos más frecuentes fueron dermatofitosis (34; 22.07%), abscesos subcutáneos (16; 10.38%) y síndrome de piel atópica (20; 10.38%), en concordancia con la frecuencia de otros lugares; si bien el orden de incidencia de los diagnósticos frecuentes fue diferente.

Effect of alginate coating activated by solid lipid nanoparticles containing Zataria multiflora essential oil on chicken fillet's preservation.

The current study aimed to assess the chemical, microbial, and sensory properties of Solid Lipid Nanoparticles (SLNs) in chicken fillets stored at 4 ± 1 °C for 12 days. As a result, the optimized ZEO-SLNS sample exhibited a spherical morphology with a droplet size of 251.51 ± 1.11 nm and a PDI of 0.34 ± 0.01 under transmission electron microscopy (TEM). The encapsulation efficiency (EE) and zeta potential were approximately 55.4 % and -20.87 ± 1.39 mV, respectively. Furthermore, encapsulating ZEO in SLNS enhanced antibacterial and antioxidant activity compared to pure ZEO. As a result, the application of alginate-loaded ZEO-SLNS extended the storage time of fresh chicken fillets. Thus, the application of this edible coating showcased a remarkable ability to substantially decelerate both microbial and chemical changes in chicken fillets during cold storage conditions. This finding underscores the potential of the edible coating as an effective means to enhance the safety and quality of chicken products.

New coating containing chitosan and Hyssopus officinalis essential oil (emulsion and nanoemulsion) to protect shrimp (Litopenaeus vannamei) against chemical, microbial and sensory changes.

In this study, the effect of chitosan coating containing emulsion and nanoemulsion of Hyssopus officinalis essential oil (EO) on the chemical, microbiological and sensory characteristics of shrimp (Litopenaeus vannamei) was investigated. The minimum value of TVB-N (Total volatile basic nitrogen), TBARS (Thiobarbituric acid reactive substances), PV (peroxide value), TMA-N (Trimethylamine-nitrogen) and FFA (Free fatty acids) after 12 days were shown in NE + HEO 1% (coating containing chitosan with nanoemulsion of EO) with 20.53 mg N/100 g, 0.5 µg/kg, 0.88 MAQ peroxide/kg, 1.3 mg/100 g and 12.16 mg 100% of oleic acid, respectively. Also, minimum value of pH after 12 days was related to the CE + HEO 1% (coating containing chitosan with emulsion of EO) with 7.60. The minimum value of psychrophilic and mesophilic microbial count after 12 days were shown in NE + HEO 1%, 4.40 ± 0.36 and 4.03 ± 0.06 cfu/g, respectively. The best score of sensory evaluation was observed in the NE-HEO 1% treatment. As a result, the edible coating containing chitosan-based nanoemulsion could be effective to the preservation of shrimp's microbiological, chemical, and sensory characteristics.

Ultrasonographic measurement of the adrenal gland-to-abdominal aortic ratio as a valuable method of estimating normal adrenal size in rabbits.

A wide range has been reported for the ultrasonographic measurements of the normal adrenal gland in rabbits. Therefore, having sufficient information about the normal measurements of the adrenal gland and their relationship with indicators such as weight, sex and the diameter of the internal abdominal aorta will be of great help in diagnosing diseases of the adrenal gland. In the present study, 21 healthy adult intact rabbits were selected. The abdominal cavity of the animals was examined using ultrasound; adrenal gland parameters such as length, width, height, circumference and area on the right and left sides were measured in both sagittal and transverse planes. Additionally, the diameter of the abdominal aorta in the sagittal plane was imaged. After statistical analysis, the parameters of the adrenal glands on the right and left showed a positive significant correlation with the weight and the diameter of the abdominal aorta, although no significant correlation was found between these parameters and sex. The ratio of adrenal gland parameters to abdominal aortic diameter was calculated and the statistical analysis of the values showed that, except for the ratio of left adrenal area to aortic diameter, the other ratio of adrenal gland parameters to abdominal aortic diameter was not significantly related to weight. Therefore, these ratios can be used as suitable indicators for assessing the change in size of the adrenal gland of rabbits of different sizes. Knowing the relationship between normal adrenal measurements and the indices such as weight, sex and diameter of the abdominal aorta can help clinicians and researchers evaluate the changes in the size of the adrenal gland.

Radiographic Reference Intervals for Cardiac Size in Common Mynahs (Acridotheres tristis).

Cardiovascular diseases in the common mynah (Acridotheres tristis) often go undiagnosed due to limitations with the physical examination and common concurrent illnesses. The aim of this study was to establish accurate reference intervals for cardiac size based on radiographs for this species. All birds were considered healthy based on physical examination. High-quality ventrodorsal radiographic images were obtained from 34 healthy common mynah birds. The cardiac width (CW), thoracic width, distance between third and fourth ribs, synsacral width, coracoid width, and distance between clavicular bones were evaluated on the ventrodorsal radiographic images. Additionally, the ratio between the CW and these anatomical indices were measured. In order to evaluate the correlation between CW and the radiographic indices, a linear regression model was used. The mean ± SD for CW in the 34 birds was 16.1 ± 0.9 mm (95% confidence interval: 14.1-18 mm). Significant moderate correlation was found for CW with thoracic width (R2 = 0.37; P < 0.001), coracoid width (R2 = 0.25; P = 0.002), and the distance between the third and fourth ribs (R2 = 0.34; P < 0.001). The radiographic reference intervals calculated in this study can be used to evaluate cardiac size in the common mynah bird.

Evaluation of wound-healing efficiency of a functional Chitosan/Aloe vera hydrogel on the improvement of re-epithelialization in full thickness wound model of rat.

OBJECTIVE: Chitosan-based hydrogels as wound dressings are expected to improve the efficiency of the wound-healing process. Fabrication of the composite structure of Aloe vera and biopolymeric hydrogels has been demonstrated to promote the wound-healing process through protection against a wide spectrum of microbes, and enhanced cell adhesion and differentiation. Therefore, the present study investigates to development of improved CHO/Aloe hydrogel for improving the wound-healing process in an animal model. MATERIALS AND METHODS: CHO hydrogel with Aloe was prepared, and its properties were evaluated in terms of viscosity, antibacterial activity, and ints In-vivo wound-healing efficiency in full-thickness wounds of rat models. Physical examination of wound-healing efficiency of CHO/Aleo hydrogel was evaluated by analyzing total wound closure, recovery percentage, and the epiderm thickness of wounds. Histological evaluation was performed using hematoxylin and eosin staining to evaluate the re-epithelialization, inflammatory response, granulation tissue formation, and fibrotic tissue formation. RESULTS: The results showed a significantly higher wound-healing rate of the CHO/Aleo group compared to other groups at 3,7,14 days (p < 0.05). After 14 days of treatment, the best healing effect was observed in the CHO/Aleo gel with the highest tissue tension compared with other groups (p < 0.05). Histological findings indicated a significant difference in inflammatory response between control and treatment groups after three days of treatment (p < 0.05). Epidermal thickness was also significantly thicker in the CHO/Aleo gel group than others (p < 0.05). CONCLUSION: The present study an improved the effective topical drug-delivery system by CHO/Aloe hydrogel with the potential to reduce inflammation over time, allowing the body to recover more quickly and better re-epithelialization for improving the wound-healing procedures.

Estimation of gestational age using ultrasonography in Baluchi sheep.

The aim of this study was to evaluate the correlation between some fetal parameters measured by ultrasonography and fetal age for developing a formula which could be used for estimation of fetal age in Baluchi sheep. Placentome diameter (PD), gestation Sac diameter (GSD), biparietal diameter (BPD), straight crown-rump length (CRL), femural length (FL), occipito-snout diameter (OSD), thoracic diameter (TD) and fetal heart rate (FHR) were measured by transrectal and/or transabdominal ultrasonography. All ewes were synchronized using progesterone sponge for 6 days. On the first day, they received PGF2 and on the last day received 400 IU of equine serum gonadotrophin (eCG). All of the parameters revealed significant correlation by increasing fetal age. Except for PD, for other values linear regression curve was illustrated. BPD (between 36 - 96 days) (R2 = 0.961), CRL (R2 = 0.935), FL (R2 = 0.950), OSD (R2 = 0.981), TD (R2 = 0.975) showed high correlation with fetal age (p < 0.001). Moderate correlation was calculated for FHR (p < 0.001, R2 = 0.883). Low correlations were assessed by measurement of GSD (p = 0.018, R2 = 0.318), BPD (between 96-138 days; p = 0.038, R2 = 0.29) and PD represented the significant non-linear correlation with age (p < 0.001), maximum correlation was assessed by measurement of mean placentome wall diameter. In conclusion, OSD was recommended as the best parameter for estimating of gestational age between days 36 - 109 in Baluchi sheep because of the lowest residuals, the highest correlation coefficient and wide period of availability for imaging in gestation.

Peppermint essential oil-loaded solid lipid nanoparticle in gelatin coating: Characterization and antibacterial activity against foodborne pathogen inoculated on rainbow trout (Oncorhynchus mykiss) fillet during refrigerated storage.

The present study was conducted to determine the characterization and antibacterial activity of peppermint essential oil-loaded solid lipid nanoparticle (PEO-SLN) and its impact on the quality of trout fillet stored at 4 ± 1°C for 12 days. The SLNs were prepared through a bath sonication technique. PEO-SLNs contained 0.2% (w/v) PEO in 2% of lipid phase glycerol monostearate (GMS) and tween 80 (1% w/v) used as a surfactant in the aqueous phase. The characterization parameter of PEO-SLN was evaluated, and the antibacterial activity of PEO-SLNs was conducted under in vitro conditions. Trout samples were analyzed for inoculated Pseudomonas aeruginosa, Listeria monocytogenes, and Escherichia coli O157:H7 during refrigerated storage. The mean particle size of PEO-SLNs was 154.83 ± 1.21 nm with a polydispersity index (PDI) of 0.35 ± 0.01 and zeta potential was about -24.16 ± 0.51 mV. The results indicated that PEO-SLN had higher antibacterial activity than the free form of PEO and also when used in combination with gelatin coating (gel + PEO-SLN) had a significant effect on preventing microbial growth in trout fillets (p < 0.05). The most decreasing rate of P. aeruginosa (1.92 log CFU/g), E. coli O157:H7 (0.71 log CFU/g), and L. monocytogenes count (1.69 log CFU/g) was seen in gel + PEO-SLN. These findings illustrated that PEO-SLNs could potentially be utilized in the food industry to increase the shelf life of fish fillets.

Protective effect of chitosan-loaded nanoemulsion containing Zataria multiflora Boiss and Bunium persicum Boiss essential oils as coating on lipid and protein oxidation in chill stored turkey breast fillets.

The present study was conducted to evaluate the lipid and protein oxidation responses of cold stored turkey meat using chitosan-contained nanoemulsions supplemented with the essential oils of two aromatic plants including Zataria multiflora Boiss and Bunium persicum Boiss. Chemical traits such as total volatile basic nitrogen (TVB-N), peroxide value (PV), thiobarbituric reactive substances (TBARs), free fatty acids (FFA), fatty acid composition and TC (total carbonyl) of samples were carried out at 4°C. Moreover, their pH and sensory properties were also determined at the same conditions. The initial value of the TVB-N (2.24 mg N/100 g) reached 20.81 mg N/100 g. TVB-N values achieved for all meat samples were lower than the highest acceptable limit (28-29 mg N/100 g). In all the treatments, PV and TBARs values were increased until day 10, and afterward a decrease was observed until day 20 of storage. TBARs values of the samples (mg MDA/kg) ranged from 1.97 ± 0.04 to 4.48 ± 0.39 in CNE + ZEO 1% to 2.72 ± 0.32 to 6.66 ± 0.21 in CON at zero time and day 5, respectively. FFA and TC were enhanced at a slower rate in the treated samples. The most efficient treatment against chemical deterioration was found to be CNE + ZEO 1%. Chitosan and sonicated chitosan treatments had the highest color score and lowest odor score at zero time. The obtained results suggested coating turkey meat fillets with ZEO and BEO as an effective strategy to delay at their chemical deterioration. PRACTICAL APPLICATION: The spoilage risk of fresh products is higher than other foods. Turkey meat spoils because of biological reactions such as the oxidation of lipids and protein, the action of endogenous enzymes, and the metabolic activities of microorganisms that end in a short shelf life. The oxidation of lipids not only reduces or retards, but also inhibits by edible coatings. Edible coatings formed from bioactive compounds would effectively provide possibility of active compounds onto surface of minimally processed foods. Therefore, application chitosan-loaded nanoemulsion coating containing Zataria multiflora Boiss and Bunium persicum Boiss essential oils is recommended in food industry especially for poultry industry to increase the chemistry and sensory quality of turkey breast fillets.

Comparison of iron chelators used alone or in combination with phlebotomy in common mynahs (Acridotheres tristis) with experimental iron storage disease.

To compare the therapeutic effects of iron chelators used alone or in combination with phlebotomy on iron storage disease (ISD), 66 healthy common mynahs (Acridotheres tristis) were fed an iron-loading diet (3000 ppm) for 30 days. After confirmation of ISD, the birds were randomly divided into four treatment groups; DFO: deferoxamine (100 mg/kg SC q24 h), DFP: deferiprone (oral, 75 mg/kg), DFO + F: deferoxamine (100 mg/kg SC q24 h) with phlebotomy, and DFP + F: deferiprone (oral, 75 mg/kg) with phlebotomy. In phlebotomy-treated groups, blood sampling (1% BW) was performed weekly. At 1 and 2 months after treatments, seven birds from each group were euthanized and liver iron, copper, and zinc were analysed by ICP-OES assay. After 1 month, in all treatments, the liver amount of iron, copper, and zinc was reduced (P < 0.05) and there was no significant difference between groups. In the second month, the amount of liver iron, copper, and zinc decreased more in all groups, but this change was insignificant except in the DFP + F group (P < 0.05). These results suggest that all therapeutic protocols after 1 month effectively reduce the liver iron and there is no need to continue treatment. Otherwise, it may lead to iron deficiency, especially in birds treated with DFP + P. Since deferiprone, as an inexpensive oral chelator, effectively reduces liver iron levels without causing stress in the birds, it can be recommended as a more appropriate method for the treatment of mynahs with ISD. However, further clinical studies are needed to define the most effective treatment.RESEARCH HIGHLIGHTS Deferiprone is an optimized method for treating iron storage disease.The essential metals homeostasis is impaired in iron storage disease.

Parasitic causes of meat and organs in cattle at four slaughterhouses in Sistan-Baluchestan Province, Southeastern Iran between 2008 and 2016.

This 8-year (from 2008 to 2016) retrospective study calculated the percentage of carcass and organ (lung and liver) condemnations and estimated the direct financial costs at four slaughterhouses in Sistan-Baluchestan Province, Southeastern Iran. Each carcass and organ (lung and liver) was thoroughly examined through inspection, palpation and incision following the standard protocol. Identification of the parasites was performed macroscopically. The total direct economic loss due to meat's condemnation was estimated by adding weights of each organ or carcass part and multiplying individual organ totals by their 2016 market unit price. A total of 857,039 cattle were slaughtered during this period, 64,497 livers (7.5%), 31,401 lungs (3.6%) and the carcasses of 1,171 cattle (0.1%) were condemned due to lesions caused by parasites. The main parasitic lesions in the condemned livers were attributed to Echinococcus granulosus (4.2%), Fasciola spp. (3.1%) and Dicrocoelium dendriticum (0.1%). All the condemned lungs were due to E. granulosus (3.6%). Taenia saginata cysticerci were detected in 0.1% of inspected animals. Liver condemnation due to cystic echinococcosis was the highest in fall (4.7%, p < 0.001); while lung condemnation was the highest during spring (3.98%, p < 0.001). Liver condemnation due to Fasciola spp. was the lowest in winter (2.99%, p < 0.001). Carcass condemnation as a result of cysticercosis was the highest in summer (p < 0.001). Considering the 2016 market prices, condemnations due to the studied parasites caused direct costs estimated U.S. $ 3,191,879. To the best of our knowledge, this is the first report estimating the monetary losses due to parasitic infections in the slaughterhouses of this province. Due to the high financial impact of the studied parasites, a control programme should be implemented to decrease this impact.

Saccharomyces cerevisiae as a delivery system of Zataria multiflora Boiss. essential oil as a natural preservative for food applications: Encapsulation of Iranian Zataria multiflora Boiss. essential oil.

BACKGROUND: The following study is an evaluation of the encapsulation, stability and release profile of Iranian Zateria multiflora boiss essential oil (ZEO) in Saccharomyces cerevisiae yeast cells. Encapsulation was performed with different essential oil / yeast weight ratios at different temperatures. The encapsulation efficiency and stability of the loaded yeasts and the release profiles of carvacrol and thymol (as the main active ingredients of ZEO) were also investigated. RESULT: The encapsulation efficiencies of carvacrol and thymol at a ZEO / yeast weight ratio of 1.25 were 30.9% ± 0.01% and 44.5% ± 0.02%, respectively. Loaded yeast cells were stable during the 4-week storage period. Both carvacrol and thymol showed substantial releases of around 60% during the first hour and around 70% during the second hour at two different water temperatures, followed by steady release. CONCLUSION: Zateria multiflora boiss essential oil can be encapsulated effectively in S. cerevisiae yeast cells, refrigerated without degradation, and released efficiently. Zateria multiflora boiss essential oil encapsulated into S. cerevisiae yeast may be used as a potential preservative for the food and drug industry. © 2020 Society of Chemical Industry.

Prognostic role of follicular fluid tumor necrosis factor alpha in the risk of early ovarian hyperstimulation syndrome.

BACKGROUND: Ovarian hyperstimulation syndrome (OHSS) is an iatrogenic condition characterized by capillary hyperpermeability which can be predicted by preovulatory ovarian responses such as number of follicles. A variety of cytokines are thought to be involved in pathophysiology of this syndrome. METHODS: A prospective cohort study invloving sixty intracytoplasmic sperm injection (ICSI) patients. On the day of hCG injection, we explored the threshold of larger follicles ≥11 mm diameter with a count of ≥18 follicles for the high-risk moderate-to-severe OHSS and 13-18 follicles for the low-risk moderate-to-severe OHSS. Whereas larger follicles count of less than 13 were classified as normoresponders. Pooled follicular fluid (FF) samples of each patient were collected on the day of oocyte retrieval. Magnetic multiplex immunoassay was explored to measure the concentrations of some intrafollicular cytokines including: GM-CSF, INF-γ, TNF-α, IL-10, CXCL8/IL-8, IL-6, IL-5, IL-4, IL-2, and IL-1ß. All sixty patients underwent controlled ovarian hyperstimulation (COH) with either GnRH agonist or antagonist protocols. RESULTS: Intrafollicular TNF-α concentration was significantly different (p < 0.05) in the high-risk moderate-to-severe OHSS patients compared to low-risk moderate-to-severe OHSS patients and normoresponders. TNF-α in FF had a negative correlation with the chance of high-risk moderate-to-severe OHSS. The differences in the risk of OHSS between patients who received GnRH agonist or antagonist were not significant (p > 0.05). CONCLUSIONS: In accordance to the negative correlation of TNF-α and high risk of early OHSS, we did not expect TNF-α to play a role in increasing vascular permeability in ovarian tissues. In addition, the risk of early moderate-to-severe OHSS was not affected by different GnRH superovulation protocols.

Chitosan-loaded nanoemulsion containing Zataria Multiflora Boiss and Bunium persicum Boiss essential oils as edible coatings: Its impact on microbial quality of turkey meat and fate of inoculated pathogens.

This study was targeted to investigate the effect of chitosan-loaded nanoemulsion enriched with two types of essential oils on the microbial quality of turkey meat. To this end, the effects of essential oils of Zataria Multiflora Boiss (ZEO) and Bunium persicum Boiss (BEO) were evaluated at two concentrations (0.5% and 1% (w/v)) during 18 days of storage at 4 °C. Initially, in vitro evaluations were performed on the prepared nanoemulsions, namely essential oil nanoemulsions and chitosan-loaded nanoemulsions containing essential oils, using micro-dilution method and agar diffusion methods, respectively. Meat samples were analyzed for microbial indicators and inoculated salmonella Enteritidis, and Listeria monocytogenes during 3-day intervals. The highest reduction rate of total viable bacteria (2.06 log CFU/g), total psychrophilic (2.59 log CFU/g), Pseudomonas spp. (2.07 log CFU/g), Enterobacteriaceae (2.51 log CFU/g), lactic acid bacteria (2.51 log CFU/g), and yeast and mold count (2.10 log CFU/g) were observed in chitosan-loaded nanoemulsion containing ZEO 1%, in comparison with control samples. Moreover, the shelf life significantly increased due to the application of chitosan-loaded nanoemulsions (15-18 days), compared to that of the control group (6 days). Therefore, the edible chitosan-based nanoemulsion could play an effective role in the preservation of the microbial qualities of turkey meat.

Seroepidemiological feature of Chlamydia abortus in sheep and goat population located in northeastern Iran.

Chlamydia abortus is a Gram-negative intracellular bacteria responsible for major economic losses due mainly to infection and subsequent induction of abortion in several animal species and poses considerable public health problems in humans. This study was conducted to determine the prevalence of antibody against C. abortus in sheep and goat population of Khorasan Razavi province located in northeastern Iran. Four hundred fifty-two (271 sheep and 181 goats) sera samples from 40 sheep/goat epidemiologic units located in 11 counties were selected. Sera were assayed for antibodies against C. abortus using ELISA assay. Out of 452 sheep and goat sera, 44 [9.70% (95.00%CI: 7.10%-12.40%)] were positive for C. abortus antibodies. 28 out of 40 epidemiologic units (70.00%) and 10 out of 11 counties (91.00%), at least one seropositive sample was found. There was no significant difference between the seropositivity of sheep and goats. Age, sex, and location did not show significant relationship with the test results. The results showed that C. abortus was circulating in wide parts of Khorasan Razavi province. Considering the economic and public health importance of C. abortus, measures should be taken to help prevent its spread and to reduce the zoonotic risk of C. abortus in the studied region.

Copper, Iron, Manganese, Zinc, Cobalt, Arsenic, Cadmium, Chrome, and Lead Concentrations in Liver and Muscle in Iranian Camel (Camelus dromedarius).

Camel meat as healthy food has received much attention for human consumption. In the present study, liver and muscle from 60 camels (Camelus dromedarius) including 26 males and 34 females were sampled to determine the concentration of As, Cd, Pb, Cr, Cu, Fe, Mn, Zn, and Co using the inductively coupled plasma-optical emission spectrometry (ICP-OES). Based on the obtained results, the average content of essential or toxic elements in the liver and muscle was as follows: 111.2 ± 26.51, 38.57 ± 7.97 (Zn), 3.28 ± 0.79, 2.12 ± 0.49 (Cu), 76.98 ± 14.20, 59.34 ± 11.81 (Fe), 0.87 ± 0.22, 0.48 ± 0.12 (Mn), 0.52 ± 0.27, 0.03 ± 0.01 (Co), ND ± 0.008, 0.012 ± 0.008 (Cd), 7.06 ± 1.58, 3.90 ± 0.86 (Cr), 0.85 ± 0.043, and 0.18 ± 0.02, and 1.12 ± 0.21 (As) mg kg-1. Pb concentration was lower than the detection limit (ND). The results showed that the liver concentrations of Cu, Zn, Fe, Mn, Co, and Cr were significantly higher than those in the muscle. The association of sex, age, region, and sampling period, with a concentration of these elements, revealed that concentration of zinc in the liver and cobalt in the muscle were significantly higher in the male. Also, significantly higher cobalt and zinc concentrations in muscle were seen in the first 6 months of the year. Age-related differences in muscle concentrations were observed for cobalt. The concentration of trace elements and heavy metals in the liver and muscle samples were not correlated. Comparison of heavy metals concentration in both tissues with European Commission regulation showed that except Cd, the other heavy metals had a higher level than the EU standard. The results of this study showed that camel meat can be contaminated with heavy metals, but more investigations are needed.

Wound Healing and Antimicrobial Effects of Chitosan-hydrogel/Honey Compounds in a Rat Full-thickness Wound Model.

INTRODUCTION: Honey and chitosan have shown antimicrobial and wound healing effects. As a biocompatible and biodegradable biomaterial, chitosan has shown antimicrobial capabilities. OBJECTIVE: In this study, the effects of the incorporation of high molecular weight chitosan hydrogel on antibacterial, antifungal, and wound healing properties of honey were investigated. MATERIALS AND METHODS: The minimum inhibitory concentration of chitosan and honey were examined in pure and 3:1, 1:1, and 1:3 (v/v) compound ratios for Staphylococcus aureus, Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans. In addition, the inflammatory, granulation and fibrotic tissue formation, reepithelialization indices, and wound shrinkage effects of each treatment were evaluated and compared with saline and silver sulfadiazine. RESULTS: Chitosan/honey 1:3 was found to be effective against all 5 aforementioned germs. Honey, chitosan/honey 1:1, and chitosan/honey 1:3 showed faster wound healing and shrinkage effects. CONCLUSIONS: Incorporation of chitosan hydrogel into honey can substantially enhance its antimicrobial and wound healing effects. Chitosan-hydrogel/honey (1:3) is an optimal wound dressing formulation with ample antimicrobial and healing properties.