Proteomics analysis reveals promotion effect of 1α,25-dihydroxyvitamin D3 on mammary gland development and lactation of primiparous sows during gestation.

1α,25(OH)2VD3 is the most active form of vitamin D3 in animals, and it plays an important role in regulating mineral metabolism and reproduction. In this study, 140 crossbred gilts (Landrace × Yorkshire) were selected, randomly divided into four groups, and fed with a commercial diet supplemented with 0, 1, 2, and 4 µg/kg of 1α,25(OH)2VD3 in the form of 1α,25(OH)2VD3-glycosides. The mammary gland tissues were sampled from sows on day 114 of gestation. The production data of sows in each group were analyzed, and the colostrum quality was evaluated. Differentially abundant proteins (DAPs) in the mammary tissues were identified by tandem mass tag (TMT) technique and were verified by Western blot and parallel reaction monitoring (PRM). The results showed that 4 µg/kg 1α,25(OH)2VD3-glycosides significantly promoted the piglet birth weight, weaning weight, colostrum quality, and lactation ability of primiparous sows. The proteomics analysis showed that of the identified 53,118 peptides, 48,868 were unique peptides. A total of 5029 DAPs were identified, of which 4292 DAPs contained quantitative information. Our data indicated that 1α,25(OH)2VD3 was involved in the regulation of the mammary gland development and lactation in a dose-dependent manner through multiple pathways during gestation of primiparous sows. SIGNIFICANCE: The mammary gland is an important lactation organ of female mammals. Our research aims to reveal the effect of dietary supplementation with 1α,25(OH)2VD3 on mammary gland development and lactation of primiparous sow. This study identified potential signaling pathways and DAPs involved in regulating the mammary gland development and lactation in sows. Our findings provides theoretical basis for improving the fecundity of sows.

Proteomics analysis reveals the effect of 1α,25(OH)2VD3-glycosides on development of early testes in piglets.

1α,25(OH)2VD3 is the most active form of VD3 in animals. It plays an important role in regulating mineral metabolism but also in reproduction. Testes are the main reproductive organs of male mammals. Our research aims to reveal the effect of 1α,25(OH)2VD3-glycosides on development of early testes in piglets. 140 weaned 21-day old piglets were selected. The piglets were randomly divided into four groups and were fed a commercial diet supplemented with 0, 1, 2 and 4 µg/kg of 1α,25(OH)2VD3, provided as 1α,25(OH)2VD3-glycosides. Sixty days after the start of the experiment, at piglet age 82 days, testes were harvested. The morphology and histology of early testicular development were assessed. In addition, the proteomic TMT/iTRAQ labelling technique was used to analyse the protein profile of the testes in each group. Western blotting was applied to verify the target of differentially abundant proteins (DAPs). The analysis of morphology and histology of testes showed that a certain concentration of 1α,25(OH)2VD3-glycosides had a positive and significant effect on testicular development. And the results of proteomics analysis showed that of the identified 132,715 peptides, 122,755 were unique peptides. 7852 proteins, of which 6573 proteins contain quantitative information. Screening for DAPs focused on proteins closely related to the regulation of testicular development such as steroid hormone synthesis, steroid biosynthesis, peroxisome and fatty acid metabolism pathways. These results indicated that 1α,25(OH)2VD3 is involved in the regulation of early testicular development in piglets. At the same time, these findings provide valuable information for the proteins involved in the regulation of testicular development, and help to better understand the mechanisms of 1α,25(OH)2VD3 in regulating the development of piglets' testes.

Influence of benzoic acid in high fibre diets on nutrient digestibility and VFA production in growing/finishing pigs.

In a feeding trial with 32 crossbred gilts (13-108 kg bodyweight) the effect of benzoic acid (5 g/kg diet) in low (NDF: 150 g/kg DM) and high (NDF: 202 g/kg DM) fibre diets on performance and apparent nutrient digestibility was examined. The animals were restrictively fed one of four grower and finisher diets: low fibre diet without and with benzoic acid (LF- and LF+), and a high fibre diet without and with benzoic acid (HF- and HF+). At 56 kg bodyweight, four animals per diet were slaughtered to obtain data on volatile fatty acid (VFA) production in the gut. In the grower period, digestibility of nitrogen, energy and neutral detergent fibre was positively influenced by benzoic acid (p < 0.01) and reduced by fibre addition (p < 0.01). The concentration of butyric acid in caecum and colon was increased by benzoic acid (p < 0.05). Dietary fibre content did not influence VFA concentrations. It is concluded that the addition of benzoic acid helped animals to utilise high fibre diets better.

Influence of benzoic acid and dietary protein level on performance, nitrogen metabolism and urinary pH in growing-finishing pigs.

An experiment was conducted to examine the effect of benzoic acid and two dietary protein levels on pig performance, nitrogen balance and urinary pH. A total of 24 crossbred barrows (26 kg to 106 kg BW) received one of four diets: low protein level with and without 1% benzoic acid (LP- and LP+, respectively) and high protein level with and without 1% benzoic acid (HP- and HP+, respectively). The animals were fed restrictively grower and finisher diets and were kept in metabolic cages in weeks 3, 6, 9, and 12 of the experiment. The addition of benzoic acid did not improve weight gain and feed conversion ratio. N-intake and digested N were only influenced by dietary protein level (p< 0.01), while N-balance was similar in all four diets. Dietary benzoic acid improved N-digestibility in the grower period (p<0.01) but not in the finisher period. The addition of benzoic acid reduced urinary pH by about one pH-unit in both feeding periods independent of the protein level of the diet (p< 0.01) and increased the concentration of urinary hippuric acid markedly (p<0.01). The results of this study indicate a positive influence of dietary benzoic acid on pigs especially in case of feeding a low protein diet in the grower period.