RESUMO
The oral application of a newly developed ferrous bisglycinate paste for suckling piglets at a dose of 180 mg/kg body weight led to increased death rates in 10% (n = 10) of Swiss test pig breeding farms (n = 100). Necropsy examination of suckling piglets (n = 12), selected randomly from the test farms with increased death rates, demonstrated severe gastric mucosal ulceration and necrosis. Due to the presence of crystalline iron surface coating within the gastric lesions, the iron was considered as the toxic principle and cause of death. To demonstrate the direct toxicity of ferrous bisglycinate, the paste was administered experimentally to a litter of suckling piglets (n = 11). Different time points (24, 48 and 72 h post partum) and doses (180 mg/kg and 360 mg/kg) were investigated. The manufacturer's recommended dose of 180 mg/kg corresponded to approximately 36 mg Fe/kg and to 6.4 % of the acute lethal dose50 of oral iron in rats. In all piglets the lesions were reproduced and most severe at the earliest time point (24 h post partum) and with the highest applied dose (360 mg/kg). The lesions were in accordance with those described from oral iron intoxication in man, suggesting pigs as an ideal animal model for oral iron toxicity studies.
Assuntos
Compostos Ferrosos/toxicidade , Mucosa Gástrica/efeitos dos fármacos , Mucosa Gástrica/patologia , Glicina/toxicidade , Doenças dos Suínos/induzido quimicamente , Administração Oral , Animais , Animais Recém-Nascidos , Compostos Ferrosos/administração & dosagem , Glicina/administração & dosagem , Necrose/induzido quimicamente , SuínosRESUMO
In a representative cross-sectional study during 12 months of the years 2008/2009 in four abattoirs in Switzerland, lung and pleura lesions as well as lesions of slaughter carcasses and organs of 34 706 pigs were studied for frequency and type of macroscopic lesions. Of the 24276 examined pigs, 91.2% of the lungs, 94.4% of the heart and 95.5% of the livers showed no macroscopically visible lesions. Pigs that were produced for a label program had significantly less bronchopneumonia and pneumonia residuals, pleuritis and liver lesions due to echinococcosis. Pigs supervised by the Swiss Pig Health Service (SGD), showed significantly less bronchopneumonia and pneumonia residuals, diffuse pleuritis, pleuritis/pericarditis and milkspots compared to the non-SGD supervised farms. Thanks to the national eradication program for enzootic pneumonia (EP) and actinobacillosis, the health-status of lungs has been considerably improved and the prevalence of pleurisy decreased considerably. The results of this study indicate a good herd health in Swiss pig production.
Assuntos
Actinobacilose/prevenção & controle , Pulmão/patologia , Pleura/patologia , Pneumonia Suína Micoplasmática/prevenção & controle , Doenças dos Suínos/patologia , Doenças dos Suínos/prevenção & controle , Matadouros , Animais , Estudos Transversais , Fígado/patologia , Miocárdio/patologia , Suínos , SuíçaRESUMO
Enteric Escherichia coli infections are a highly relevant cause of disease and death in young pigs. Breeding genetically resistant pigs is an economical and sustainable method of prevention. Resistant pigs are protected against colonization of the intestine through the absence of receptors for the bacterial fimbriae, which mediate adhesion to the intestinal surface. The present work aimed at elucidation of the mode of inheritance of the F4ad receptor which according to former investigations appeared quite confusing. Intestines of 489 pigs of an experimental herd were examined by a microscopic adhesion test modified in such a manner that four small intestinal sites instead of one were tested for adhesion of the fimbrial variant F4ad. Segregation analysis revealed that the mixed inheritance model explained our data best. The heritability of the F4ad phenotype was estimated to be 0.7±0.1. There are no relations to the strong receptors for variants F4ab and F4ac. Targeted matings allowed the discrimination between two F4ad receptors, that is, a fully adhesive receptor (F4adRFA) expressed on all enterocytes and at all small intestinal sites, and a partially adhesive receptor (F4adRPA) variably expressed at different sites and often leading to partial bacterial adhesion. In pigs with both F4ad receptors, the F4adRPA receptor is masked by the F4adRFA. The hypothesis that F4adRFA must be encoded by at least two complementary or epistatic dominant genes is supported by the Hardy-Weinberg equilibrium statistics. The F4adRPA receptor is inherited as a monogenetic dominant trait. A comparable partially adhesive receptor for variant F4ab (F4abRPA) was also observed but the limited data did not allow a prediction of the mode of inheritance. Pigs were therefore classified into one of eight receptor phenotypes: A1 (F4abRFA/F4acR+/F4adRFA); A2 (F4abRFA/F4acR+/F4adRPA); B (F4abRFA/F4acR+/F4adR-); C1 (F4abRPA/F4acR-/F4adRFA); C2 (F4abRPA/F4acR-/F4adRPA); D1 (F4abR-/F4acR-/F4adRFA); D2 (F4abR-/F4acR-/F4adRPA); E (F4abR-/F4acR-/F4adR-).
Assuntos
Antígenos de Bactérias/genética , Antígenos de Bactérias/metabolismo , Aderência Bacteriana , Enterócitos/microbiologia , Escherichia coli Enterotoxigênica/metabolismo , Infecções por Escherichia coli/veterinária , Proteínas de Escherichia coli/genética , Proteínas de Escherichia coli/metabolismo , Proteínas de Fímbrias/genética , Proteínas de Fímbrias/metabolismo , Doenças dos Suínos/microbiologia , Animais , Enterócitos/patologia , Epistasia Genética , Infecções por Escherichia coli/patologia , Feminino , Interações Hospedeiro-Patógeno , Masculino , Sus scrofa , Suínos , Doenças dos Suínos/genética , Doenças dos Suínos/patologiaRESUMO
The objectives of this study were a description of the practical implementation of the painless castration under inhalational anaesthesia with an objective assessment of animal welfare, workplace safety and time exposure. 600 questionnaires were sent to farmers all over Switzerland and 100 farms were visited during castration under inhalation anaesthesia. 44 % of the visited farmers administered analgetics during anaesthesia or less than 10 minutes before castration. 14 % of the piglets were insufficiently anaesthetised (moving or vocalising) and 18 % showed stronger bleeding tendency after castration. The mortality rate was less than 0.1 %. 22 % of the swine farmers reported headache or dizziness during or after castration work. The Isoflurane level on 2 farms was above the Swiss safety limits. The time needed for castration was with 4.3 minutes clearly above the time necessary without anaesthesia. The additional financial costs and time are at the moment not adequately compensated to the farmers.
Le but de la présente étude était de décrire la mise en application en Suisse de la castration indolore des porcelets sous anesthésie par inhalation et d'étudier les aspects de protection des animaux, de sécurité à la place de travail ainsi que de l'investissement en temps pour cette castration. On a envoyé 600 questionnaires à des producteurs de porcelets dans toute la Suisse (taux de retour 40.5 %) et 100 exploitations ont été visitées lors de castrations sous anesthésie par inhalation. Sur 44 % des exploitations visitées, un analgésique était appliqué soit pendant l'anesthésie soit moins de 10 minutes avant la castration. 14 % des porcelets montraient des réactions de défense ou des vocalisations causées par la castration et 18% présentaient une tendance augmentée aux saignements après la castration. La mortalité était inférieure à 0.1 %. 22 % des exploitants mentionnaient des céphalées ou des vertiges pendant ou après les castrations. Sur deux exploitations, les concentrations limites d'isoflurane admises en Suisse étaient dépassées. Le temps nécessaire pour une castration, y compris le pré- et le postopératoire, était, avec 4,3 minute, nettement supérieur à celui nécessaire pour une castration sans anesthésie. Les producteurs ne sont actuellement pas correctement indemnisés pour les surcoûts et l'investissement supplémentaire en temps.
Assuntos
Analgésicos/administração & dosagem , Anestesia por Inalação/veterinária , Orquiectomia/veterinária , Dor/veterinária , Suínos/cirurgia , Anestesia por Inalação/métodos , Bem-Estar do Animal/normas , Animais , Masculino , Saúde Ocupacional , Orquiectomia/efeitos adversos , Dor/etiologia , Dor/prevenção & controle , Suíça , Fatores de TempoRESUMO
The aim of this study was to describe the practical implementation of the painless castration under injection anaesthesia in Switzerland. 30 swine farms were visited and 60 farmers answered a questionnaire. 34 % of the piglets showed movements during castration under injection anaesthesia and 17 % had excitations during recovery from anaesthesia. After 48 minutes half of the piglets were in sternal position and after 112 minutes half of them showed coordinated movements. The body temperature decreased by 3.1 °C until 60 minutes after castration, especially small piglets reached critical temperature levels. 38 % of the piglets showed strong bleeding after castration. The healing of the wound was good according to 82 % of the farmers. 83 % of the farmers reported piglet losses, especially at the beginning of the anaesthesia period. The anaesthesia may be improved by using butorphanol in addition to the combination of ketamine and azaperone. The recovery of the piglets should be in a warm place without any risk of injury by obstacles or the sow. Increased bleeding can be controlled by using an emasculator.
Le but de la présente étude était de juger de la mise en application en Suisse de la castration indolore des porcelets au moyen d'une anesthésie par injection. On a visite 30 exploitations choisies au hasard et 60 ont été interrogées par le biais d'un questionnaire. Lors des visites, 34 % des 371 porcelets observés ont présenté de fortes réactions de défense en relation avec la castration et 17 % une importante excitation durant la phase de réveil. Il fallait en moyenne 48 minutes pour que la moitié des animaux soient en position sternale et 112 minutes pour que la moitié puissent à nouveau marcher de façon coordonnée. La température corporelle s'abaissait en moyenne de 3.1 °C 60 minutes apres la castration, les plus petits des porcelets atteignant tout particulièrement des valeurs critiques. 38 % des porcelet saignaient fortement après la castration. La cicatrisation était bonne dans 83% des exploitations questionnées. 83 %. 83 % des exploitants se plaignaient de pertes, survenant en particulier lors de la phase de reveil. L'anesthésie avec de la kératine et de l'azaperon peut être améliorée par l'adjonction de butorphanol. Pour la phase de réveil, les porcelets doivent être placés, séparés de leur mère, dans un environnement chaud. On peut diminuer les saignement postopératoires par l'usage d'un émasculateur.
Assuntos
Anestesia/veterinária , Anestésicos/administração & dosagem , Orquiectomia/veterinária , Dor/veterinária , Suínos/cirurgia , Anestesia/métodos , Período de Recuperação da Anestesia , Animais , Temperatura Corporal , Injeções Intramusculares/veterinária , Masculino , Orquiectomia/efeitos adversos , Dor/prevenção & controle , Inquéritos e Questionários , Suíça , CicatrizaçãoRESUMO
In Switzerland postweaning multisystemic wasting syndrome (PMWS), caused by porcine circovirus type 2, was detected for the first time in 2001. To comprise the PMWS epizooty in 2003 - 2006 retrospectively, individual animals were diagnosed according to internationally accepted criteria and temporal and regional patterns of the epizooty were reconstructed. Occurrence of PMWS was predominantly in regions with a high frequency of swine farms (central and eastern Switzerland). Apparently it was spread to other, less affected regions, through trade of infected fattening pigs. Concurrently, disease was found in different establishments of production. Affected were mainly weaners or fattening pigs. In 40 % of the breeding farms and in 25 % of the fattening farms mortality rate was higher than 5 %. Starting in 2003, also a higher frequency of porcine dermatitis and nephropathy syndrome (PDNS) diseased pigs was diagnosed. In the years 2004 to 2006 they accounted for about 10 % of the diagnosed PCV2-associated diseases. Besides the characteristic skin- and kidney lesions approximately half of the PDNS cases showed wasting and lymphoid lesions with high quantities of PCV2 antigen. We termed these mixed forms PMWS-PDNS-hybrid forms.
Assuntos
Dermatite/veterinária , Síndrome Definhante Multissistêmico de Suínos Desmamados/epidemiologia , Doenças dos Suínos/epidemiologia , Animais , Suínos , Suíça/epidemiologiaRESUMO
Postweaning multisystemic wasting syndrome (PMWS) was epizoozic between 2003 and 2008 in Switzerland. Nevertheless, infectious risk factors including porcine reproductive and respiratory syndrome virus (PRRSV) were missing at all or were seen only sporadically (enzootic pneumonia and actinobazillosis). In a case-control study, 30 farms with PMWS affected pigs were compared to 30 inconspicious farms ("matched pairs"). The case-control allocation was verified by PCV2 DNA measurements of 5 healthy weaned pigs in each control farm, 5 healthy and 5 PMWS affected weaners in each PMWS affected farm. Diseased pigs showed in average 1.8x10(8) DNA templates per ml serum significantly higher than healthy pigs from control farms with 1x10(6) DNA templates per ml serum. Virus load in healthy pigs did not differ between control- and PMWS affected farms. PMWS mainly emerged among affected pigs in the 5th to 8th week of age. In a logistic regression model risk factors were identified such as high occupancy in weaning pens (p = 0.002), large groups in gestation facilities (p = 0.03) as well as reduced birth weight < 1.3 kg (p = 0.04). We suggest these factors might have lead to chronic stress e.g. through influencing negatively social interaction in pigs or disturbances of the maturing immune system. Heavy fly and rodent infestation might not only be viewed as a vector for disease transmission, but, also as a stress factor.
Assuntos
Síndrome Definhante Multissistêmico de Suínos Desmamados/epidemiologia , Animais , Estudos de Casos e Controles , DNA Viral/sangue , Síndrome Definhante Multissistêmico de Suínos Desmamados/sangue , Síndrome Definhante Multissistêmico de Suínos Desmamados/virologia , Fatores de Risco , Sus scrofa , Suínos , Suíça/epidemiologia , Carga ViralRESUMO
The following case report describes a selenium toxicosis in a pig-fattening farm of two finisher groups. The diseased animals partly showed ataxia and paresis or intense lameness in connection with band-like ablation of the epidermis at the coronary band. Some of them suffered from alopecia. Foot-and-mouth disease and swine vesicular disease were excluded by serological tests. Dissection revealed a multifocal bilateral symmetric poliomyelomalacia. Histological changes in the claws ranged from severe cell-decay in the germinative layer to distinctive decay of the stratum corneum. Due to damage of the claw epidermis the corium was partly exposed. Feed analysis revealed 100-fold increased selenium content in the finishing premix from the feed mill and as a result 20- to 60-fold increased selenium values in feed samples from the farm-made finisher mixture. Selenium concentration in the blood of diseased animals was 4- to 10-fold higher than normal values, which confirmed the tentative diagnosis of a selenium toxicosis.
Assuntos
Ração Animal/intoxicação , Selênio/intoxicação , Doenças dos Suínos/patologia , Animais , Ataxia/induzido quimicamente , Ataxia/veterinária , Casco e Garras/patologia , Paresia/induzido quimicamente , Paresia/veterinária , Selênio/sangue , Suínos , Doenças dos Suínos/sangueRESUMO
Diarrhoea in newborn and weaned pigs caused by enterotoxigenic Escherichia coli (ETEC) expressing F4 fimbriae leads to considerable losses in pig production. In this study, we refined the mapping of the receptor locus for ETEC F4ab/F4ac adhesion (F4bcR) by joint analysis of Nordic and Swiss data. A total of 236 pigs from a Nordic experimental herd, 331 pigs from a Swiss experimental herd and 143 pigs from the Swiss performing station were used for linkage analysis. Genotyping data of six known microsatellite markers, two newly developed markers (MUC4gt and HSA125gt) and an intronic SNP in MUC4 (MUC4-8227) were used to create the linkage map. The region for F4bcR was refined to the interval SW207-S0075 on pig chromosome 13. The most probable position of F4bcR was in the SW207-MUC4 region. The order of six markers was supported by physical mapping on the BAC fingerprint contig from the Wellcome Trust Sanger Institute. Thus, the region for F4bcR could be reduced from 26 to 14 Mb.
Assuntos
Aderência Bacteriana/genética , Infecções por Escherichia coli/veterinária , Proteínas de Escherichia coli/genética , Proteínas de Fímbrias/genética , Fímbrias Bacterianas/metabolismo , Doenças dos Suínos/genética , Doenças dos Suínos/microbiologia , Animais , Mapeamento Cromossômico/veterinária , Cromossomos/genética , Cromossomos/metabolismo , Infecções por Escherichia coli/genética , Proteínas de Escherichia coli/metabolismo , Proteínas de Fímbrias/metabolismo , Marcadores Genéticos/genética , SuínosAssuntos
Ataxia/genética , Proteínas de Membrana Transportadoras/genética , Paraparesia Espástica/genética , Monoéster Fosfórico Hidrolases/genética , Sus scrofa , Doenças dos Suínos/genética , Animais , Sequência de Bases , Dados de Sequência Molecular , Mapeamento de Híbridos Radioativos , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Análise de Sequência de DNA/veterinária , SuínosRESUMO
Based on Directive (EC) No 99/2003, monitoring programs on the development of antimicrobial resistance in bacteria from livestock are implemented in many European countries. The aim of the present study was (i) to establish comprehensive baseline data on the antimicrobial resistance situation in Escherichia coli isolates obtained from healthy pigs (pooled fecal samples) originating from 60 Swiss pig-breeding farms, and (ii) to analyze differences in the resistance frequency between Escherichia coli isolates from weaned pigs and sows. Susceptibility testing (disc diffusion method) was performed on 429 isolates from weaned pigs and 431 isolates from sows. Overall, 17.7% of the isolates from weaned pigs and 22.5% of the Escherichia coli isolates from sows were susceptible to all antibiotics tested. Low resistance prevalence was found for amoxicillin, amoxicillin/clavulanic acid, ampicillin, cefquinome, ciprofloxacin, colistin, florfenicol, and gentamicin. The most frequently found resistances were against streptomycin (60.6% of the isolates from weaners and 64.3% of the isolates from sows), sulfonamide (51.5% and 26.9%), tetracycline (35.2% and 22.0%), and trimethoprim (27.5% and 11.1%). With exception of colistin, most resistances were found for those antibiotics commonly used on the farms. Except for ciprofloxacin and streptomycin, isolates from weaned pigs showed higher resistance prevalence than those from sows. This difference was significant for cefquinome, florfenicol, sulfonamide, tetracycline, and trimethoprim (p<0.05).
Assuntos
Antibacterianos/uso terapêutico , Farmacorresistência Bacteriana , Infecções por Escherichia coli/veterinária , Escherichia coli/efeitos dos fármacos , Doenças dos Suínos/tratamento farmacológico , Animais , Relação Dose-Resposta a Droga , Farmacorresistência Bacteriana Múltipla , Escherichia coli/crescimento & desenvolvimento , Infecções por Escherichia coli/tratamento farmacológico , Fezes/microbiologia , Feminino , Masculino , Testes de Sensibilidade Microbiana/veterinária , Suínos , Doenças dos Suínos/microbiologia , Suíça , Resultado do TratamentoRESUMO
In boars, scrotal haemangiomas have been described frequently, but intratesticular haemangiomas are rare. Of 12 boars with scrotal haemangiomas, three animals also had testicular tumours, as follow: testicular haemangioma (TH) (n=1); TH with intratubular germ cell tumour (ITGT) (n=1); TH with intratubular germ cell-like tumour (ITGLT) and Sertoli cell tumour (n=1). In the nine remaining boars, no testicular tumours were found. Immunohistochemical examination of scrotal and testicular haemangiomas revealed labelling of endothelial cells for vimentin and factor VIII-related antigen. Labelling of smooth muscle actin was occasionally observed in the walls of neoplastic vessels. In the ITGT, no labelling was produced by any of the antibodies used. The Sertoli cell tumour was strongly positive for S-100. Sperm granulomas and degeneration of seminiferous tubules were also observed.
Assuntos
Hemangioma Capilar/patologia , Hemangioma Capilar/veterinária , Escroto/patologia , Doenças dos Suínos/patologia , Neoplasias Testiculares/patologia , Neoplasias Testiculares/veterinária , Animais , Hemangioma Capilar/metabolismo , Imuno-Histoquímica , Masculino , Sus scrofa , Doenças dos Suínos/metabolismo , Neoplasias Testiculares/metabolismoRESUMO
The cause of porcine congenital progressive ataxia and spastic paresis (CPA) is unknown. This severe neuropathy manifests shortly after birth and is lethal. The disease is inherited as a single autosomal recessive allele, designated cpa. In a previous study, we demonstrated close linkage of cpa to microsatellite SW902 on porcine chromosome 3 (SSC3), which corresponds syntenically to human chromosome 2. This latter chromosome contains ion channel genes (Ca(2+), K(+) and Na(+)), a cholinergic receptor gene and the spastin (SPG4) gene, which cause human epilepsy and ataxia when mutated. We mapped porcine CACNB4, KCNJ3, SCN2A and CHRNA1 to SSC15 and SPG4 to SSC3 with the INRA-Minnesota porcine radiation hybrid panel (IMpRH) and we sequenced the entire open reading frames of CACNB4 and SPG4 without finding any differences between healthy and affected piglets. An anti-epileptic drug treatment with ethosuximide did not change the severity of the disease, and pigs with CPA did not exhibit the corticospinal tract axonal degeneration found in humans suffering from hereditary spastic paraplegia, which is associated with mutations in SPG4. For all these reasons, the hypothesis that CACNB4, CHRNA1, KCNJ3, SCN2A or SPG4 are identical with the CPA gene was rejected.
Assuntos
Ataxia/veterinária , Canais Iônicos/genética , Paresia/veterinária , Doenças dos Suínos/genética , Animais , Anticonvulsivantes/administração & dosagem , Anticonvulsivantes/uso terapêutico , Ataxia/tratamento farmacológico , Ataxia/genética , Mapeamento Cromossômico , Cromossomos de Mamíferos , Etossuximida/administração & dosagem , Etossuximida/uso terapêutico , Fases de Leitura Aberta , Paresia/tratamento farmacológico , Paresia/genética , Fenótipo , Análise de Sequência de DNA , Suínos , Doenças dos Suínos/congênito , Doenças dos Suínos/tratamento farmacológicoRESUMO
Chlamydiae cause abortion and reproductive disorders in sows. Although organisms can infect the male genital tract, little is known about the disease situation in boars. Hence, we examined the prevalence of chlamydial infection in semen and genital tracts of boars. Samples collected from Swiss boars (group A: n=42), and boars from Germany (group B: n=39) were examined by bacteriology, LPS-ELISA, immunohistochemistry (IHC) and polymerase chain reaction (PCR). The latter methodology involved use of three PCR assays including 16Sig rDNA, IGS-S (intergenic spacer 16S/23S-Short) and IGS-L (intergenic spacer 16S/23S-Long) PCR for comparison methods. PCR sensitivity and the presence of potential PCR inhibitors were determined by spiking semen with Chlamydophila (Cp.) abortus DNA. Detection limits of the 16Sig and IGS-S PCR were 10 templates, while the IGS-L PCR was less sensitive (100 templates). Of 25 semen samples that were collected from group A, one semen sample was positive for Cp. psittaci and two were positive for Chlamydia-like organisms by 16Sig PCR. Screening of sera from Swiss boars revealed three animals with positive reactions in the LPS-ELISA, although we failed to detect chlamydiae within organs of these or sera-negative animals by IHC or IGS-S PCR. In group B, 10 ejaculates were positive for Chlamydia (C.) suis and two were positive for Chlamydia-like organisms by 16S PCR. The identification of DNA from Chlamydia-like organisms in semen from both groups of boars was surprising and a role for these bacteria in reproductive diseases requires further assessment. In conclusion, the prevalence of chlamydial infection was low in group A animals indicating that venereal transmission may not be significant for Chlamydia-associated reproductive diseases in pigs, although rare cases may occur.
Assuntos
Infecções por Chlamydia/veterinária , Chlamydia/isolamento & purificação , Genitália Masculina/microbiologia , Sêmen/microbiologia , Doenças dos Suínos/microbiologia , Animais , Infecções por Chlamydia/diagnóstico , Infecções por Chlamydia/epidemiologia , Infecções por Chlamydia/microbiologia , DNA Bacteriano/isolamento & purificação , Masculino , Reação em Cadeia da Polimerase/veterinária , Prevalência , Suínos , Doenças dos Suínos/epidemiologia , SuíçaRESUMO
Susceptibility to enterotoxigenic Escherichia coli with fimbriae F4ac is dominantly inherited in the pig. A three-generation pedigree was created to refine the position of F4acR on chromosome 13 comprising 202 pigs: eight parents, 18 F1 and 176 F2 pigs. The 17-point analysis indicates that F4acR lies between Sw207 and S0283. Recombinant offspring specify that the most probable order is Sw207-S0075-F4acR-Sw225-S0283. We observed six phenotypes for the three fimbrial variants F4ab, F4ac and F4ad. The two missing phenotypes F4abR-/F4acR+/F4adR+ and F4abR-/F4acR+/F4adR- indicate that pigs susceptible to F4ac are always susceptible to F4ab. Furthermore, a weak and a strong adhesion of F4ab and F4ad bacteria was observed. The weak receptor F4abR (F4abRw) was present only in pigs devoid of the receptor F4acR (F4abR+/F4acR-). In contrast, in pigs with the phenotype F4abR+/F4acR+, F4ab bacteria adhered to the majority of enterocytes. F4abRw constitutes a frequently observed phenotype whose inheritance is still unclear. Strong adhesion of F4ab and F4ac bacteria is most likely influenced by the same receptor that we name F4bcR. The number of F4ad bacteria that adhered to enterocytes was very variable in the adhesion test. Moreover, expression of F4adR was independent of age. Our segregation analyses indicated a dominant inheritance of F4adR, although the number of susceptible pigs was smaller than expected. We examined four genes as candidates for the F4acR locus: the transferrin receptor gene (TFRC) and three genes members of the glucosyl/galactosyltransferase family (B3GnT5, B3GALT3 and B4GALT4). Comparison of sequences from resistant and homozygous susceptible F4ac pigs did not reveal any causative single nucleotide polymorphism in the four genes. Two silent mutations at the positions 295 (C/T) and 313 (T/C) in B3GALT3 were found. Using the somatic cell hybrid panel, B3GnT5 and B3GALT3 were assigned to the chromosomal region SSC13q23-q41. No mutations were found in the cDNA sequences of these genes associated with the F4acR genotypes.
Assuntos
Antígenos de Bactérias/genética , Aderência Bacteriana/genética , Infecções por Escherichia coli/veterinária , Proteínas de Escherichia coli/genética , Proteínas de Fímbrias/genética , Predisposição Genética para Doença/genética , Doenças dos Suínos/genética , Doenças dos Suínos/microbiologia , Animais , Antígenos de Bactérias/metabolismo , Sequência de Bases , Mapeamento Cromossômico/veterinária , Cromossomos de Mamíferos/genética , Primers do DNA , DNA Complementar/genética , Infecções por Escherichia coli/genética , Infecções por Escherichia coli/microbiologia , Proteínas de Escherichia coli/metabolismo , Proteínas de Fímbrias/metabolismo , Intestinos/microbiologia , Escore Lod , Dados de Sequência Molecular , Linhagem , Fenótipo , Reação em Cadeia da Polimerase Via Transcriptase Reversa/veterinária , Análise de Sequência de DNA/veterinária , SuínosRESUMO
The purpose of the study was to find out if the supplementation of phytase to a diet of gestating and lactating sows has any effects on performance and bone parameters of the animals. Forty primiparous gilts were assigned into four groups: group A with phytase [4.2 g total phosphorus (P)/kg (gestation) and 4.5 g total P/kg (lactation)], group B without phytase (with phytase supplementation in diet for rearing) and same P content as group A, group C without phytase and higher P contents [5.0 g total P/kg (gestation) and 5.5 g total P/kg (lactation)] and group D with the same diet as group B (no phytase during the rearing). A 6-phytase was used in this trial (750 FTU/kg diet). The four diets were fed during gestation and lactation. Faeces were collected to determine apparent digestibility of minerals. Blood samples were taken to analyse minerals and bone markers. After weaning the sows were slaughtered and the bones of one hind leg were prepared to measure bone mineral density (BMD) and bone mineral content (BMC) of the tibia. Bone ash and mineral content of the phalanx III were determined. Mean P concentrations in serum decreased during gestation and lactation. But there were no significant differences between the groups. Bone formation marker bone-specific alkaline phosphatase decreased at the beginning of lactation whereas bone resorption marker serum crosslaps increased. The BMD and BMC of the tibia were slightly higher in the groups fed higher concentrations of P and phytase. The ash and mineral contents of the phalanx were the highest for the group fed the highest concentration of P. The apparent digestibility of P increased during gestation mostly in group A (57%--> 69%). In conclusion, high P content and addition of phytase to the diet induced a slightly higher ash content of the bones. It is of high importance, that sows during gestation absorb enough P, to avoid lamenesses and sudden fractures. As not many studies with phytase have been performed during gestation and lactation in sows yet, we can recommend, that phytase as supplement can be used to keep P in the diet at a lower level without negative consequences for bone health.
Assuntos
6-Fitase/administração & dosagem , Osso e Ossos/metabolismo , Dieta Vegetariana , Lactação/metabolismo , Fósforo na Dieta/administração & dosagem , Suínos/fisiologia , Fosfatase Alcalina/metabolismo , Ração Animal , Fenômenos Fisiológicos da Nutrição Animal , Animais , Densidade Óssea/efeitos dos fármacos , Densidade Óssea/fisiologia , Desenvolvimento Ósseo/fisiologia , Reabsorção Óssea/metabolismo , Osso e Ossos/efeitos dos fármacos , Suplementos Nutricionais , Feminino , Necessidades Nutricionais , Fósforo na Dieta/sangue , Gravidez , Distribuição Aleatória , Suínos/crescimento & desenvolvimento , Suínos/metabolismo , Aumento de PesoRESUMO
OBJECTIVE: Because of known significant seasonal fluctuations of iodine concentration in cow's milk (ICM) in Switzerland (winter/summer (w/s) ICM ratio averages 5.6), we looked for seasonal variations and familial aspects in urinary iodine and sodium concentrations (UIC, UNaC). DESIGN: : Prospective sequential cohort investigation. SETTING: University hospital. SUBJECTS: Thirteen children (six girls, seven boys) aged 3-10 y and their parents (n=9) aged 30-47 y. INTERVENTIONS: The volunteers collected 1729 spot urine samples (5.1996-5.1998). UIC data from winter (UIC(w): December-February) and summer seasons (UIC(s): July-September) were compared with UNaC and average milk consumption. Iodine intakes from milk and salt were modelized. RESULTS: Highest seasonal fluctuations of UIC were found in six pre-school children (w/s UIC group average=1.56, significant), followed by seven school children (w/s UIC group average=1.24, N.S.); none existed in adults. UIC/UNaC showed corresponding seasonal fluctuations in children, but not in adults. Winter milk was an important iodine source for children, as proven indirectly by similar seasonal fluctuations of ICM, UIC, UIC/UNaC and an important part within UIC due to milk. CONCLUSIONS: Contribution to UIC from milk intake during winter was high in children (40-50%) and lower in adults (about 20%). Compared with children, dietary habits of adults are more complex and their iodine supply depends mainly on iodized salt and not on milk, so the effect of seasonal ICM variations on UIC is less marked. Because of significant seasonal UIC fluctuations in consumers of fresh milk products in Switzerland, results of future UIC studies conducted during summer and winter seasons should be compared cautiously, especially in young children. Furthermore, consumption of milk is to be promoted, since, besides calcium and vitamins, it is an essential source of iodine. UNaC determination should be included in epidemiologic studies if the dietary source of iodine is questioned.
Assuntos
Fatores Etários , Dieta , Iodo/administração & dosagem , Iodo/urina , Estações do Ano , Adulto , Animais , Criança , Pré-Escolar , Estudos de Coortes , Laticínios , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Leite , Estudos Prospectivos , Sódio/urina , Suíça , UrinaRESUMO
The aim of this study was to refine the localization of the receptor locus for fimbriae F4ac. Small intestinal enterocyte preparations from 187 pigs were phenotyped by an in vitro adhesion test using two strains of Escherichia coli representing the variants F4ab and F4ac. The three-generation pedigree comprised eight founders, 18 F1 and 174 F2 animals, for a total of 200 pigs available for the linkage analysis. Results of the adhesion tests on 171 F2 pigs slaughtered at 8 weeks of age show that 23.5% of the pigs were adhesive for F4ab and non-adhesive for F4ac (phenotype F4abR+/F4acR-; R means receptor). Pigs of this phenotype were characterized by a weak adhesion receptor for F4ab. No pigs were found expressing only F4acR and lacking F4abR. Receptors for F4ab and F4ac (F4abR+/F4acR+) were expressed by 54.5% of the pigs. Animals of this phenotype strongly bound both F4ab and F4ac E. coli. In the segregation study, the serum transferrin (TF) gene and 10 microsatellites on chromosome 13 were linked with F4acR (recombination fractions (theta) between 0.00 and 0.11 and lod score values (Z) between 11.4 and 40.4). The 11-point analysis indicates the F4acR locus was located in the interval S0068-Sw1030 close to S0075 and Sw225, with recombination fractions (theta) of 0.05 between F4acR and S0068, 0.04 with Sw1030, and 0.00 with S0075 and Sw225. The lack of pigs displaying the F4abR-/F4acR+ phenotype and the presence of two phenotypes for F4abR (a strong receptor present in phenotype F4abR+/F4acR+ and a weak receptor in phenotype F4abR+/F4acR-) led us to conclude that the receptor for F4ac binds F4ab bacteria as well, and that it is controlled by one gene localized between S0068 and Sw1030 on chromosome 13.
Assuntos
Aderência Bacteriana/genética , Infecções por Escherichia coli/veterinária , Escherichia coli/metabolismo , Fímbrias Bacterianas/metabolismo , Doenças dos Suínos/microbiologia , Suínos/genética , Animais , Mapeamento Cromossômico/veterinária , Cruzamentos Genéticos , Infecções por Escherichia coli/genética , Infecções por Escherichia coli/microbiologia , Feminino , Fímbrias Bacterianas/microbiologia , Ligação Genética , Técnicas In Vitro , Enteropatias/genética , Enteropatias/microbiologia , Enteropatias/veterinária , Mucosa Intestinal/metabolismo , Mucosa Intestinal/microbiologia , Masculino , Repetições de Microssatélites/genética , Fenótipo , Reação em Cadeia da Polimerase/veterinária , Doenças dos Suínos/genética , Doenças dos Suínos/metabolismo , Transferrina/genética , Transferrina/metabolismoRESUMO
This study was conducted to examine if substantial bone loss occurs in growing pigs fed a vegetarian diet in comparison with a diet containing fishmeal. Twelve 6-week-old weaned pigs were assigned to two groups: group V [vegetarian diet; 0.61% phosphorus (P) in dry matter until 25 kg and 0.46% P until the end of the experiment] and group F (fishmeal diet; 0.61% P in dry matter until 25 kg and 0.46% P until the end of the experiment). Phytase was added to both diets. These two diets were fed to the two groups for a period of 6 weeks. Blood samples were collected weekly, faeces were collected three times a week. Concentrations of osteocalcin (OC) and carboxyterminal telopeptide of type I collagen (ICTP) were measured in serum, using a radioimmunoassay, and bone-specific alkaline phosphatase (bAP) was measured using an enzyme immunoassay. Bone mineral density (BMD) and content (BMC) were determined by peripheral quantitative computer tomography (pQCT) in the tibia and phalanx. In addition, 1,25-dihydroxyvitamin D (VitD) and parathyroid hormone (PTH) were measured in serum. The digestibility of P was significantly decreased in group V. Significant changes in bAP activities and OC concentrations occurred with time during the 6 weeks. ICTP concentrations were significantly higher in group V. Total BMC and BMD in the tibia and BMD in the phalanx significantly decreased in group V. The results show that a vegetarian diet induces a significant loss of bone and a higher bone formation in group V compared with group F, although phytase was added to both diets. The dietary requirements for P in pigs, especially in the context of feeding vegetarian diets and adding an appropriate amount of phytase, should be investigated further.
Assuntos
Fenômenos Fisiológicos da Nutrição Animal , Desenvolvimento Ósseo/fisiologia , Fósforo na Dieta/administração & dosagem , Suínos/crescimento & desenvolvimento , 6-Fitase/administração & dosagem , Fosfatase Alcalina/sangue , Ração Animal , Animais , Densidade Óssea/fisiologia , Colágeno/sangue , Colágeno Tipo I , Dieta Vegetariana , Feminino , Produtos Pesqueiros , Minerais/administração & dosagem , Minerais/metabolismo , Necessidades Nutricionais , Osteocalcina/sangue , Peptídeos/sangue , Fósforo na Dieta/metabolismo , Suínos/sangue , Fatores de TempoRESUMO
Porcine circovirus type 2 (PCV 2) was found in three five- and nine-week-old pigs from two feeder pig producer farms. Clinical signs were persistent diarrhea and wasting. The animals showed histomorphologic changes characteristic for "Postweaning Multisystemic Syndrome" (PMWS). One animal had the typical basophilic intracytoplasmatic circoviral inclusion bodies in the Peyer's patches of the ileum. Circoviral DNA was detected in the lymphatic organs as well as in the liver. This case report is the first description of PCV 2 in Switzerland.