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Stimuli-responsive compartments are attracting more and more attention through the years motivated by their wide applications in different fields including encapsulation, manipulation, and triggering of chemical reactions on demand. Among others, magnetic responsive compartments are particularly attractive due to the numerous advantages of magnetic fields compared to other external stimuli. In this article, we used an oil-based ferrofluid where the magnetic nanoparticles have been coated with different polymers to increase their amphiphilic character and surface activity, consequently rendering the interface magnetically responsive. Microliter aqueous nonmagnetic droplets dispersed in the oil-based ferrofluid were used as a model of microreactors. A comprehensive experimental and theoretical study of the deformation, attraction, and coalescence processes of the nonmagnetic water droplets coated with the magnetic nanoparticles under an applied magnetic field in the continuous oil-based ferrofluid phase is provided. To manipulate the packing of the nanoparticles at the water/oil interface, the ionic strength of the aqueous droplets was varied using different NaCl concentrations, and its effect on modulating the coalescence of the droplets was probed. Our results show that the water droplets deform along the magnetic field depending on the magnetic properties of the ferrofluid itself and on the surface properties of the interface, attract in pairs under the action of the magnetic dipole force, and coalesce by the action of the same force with a stochastic behavior. We have studied all of these phenomena as a function of the magnetic field applied, evaluating in each case the forces and/or pressures acting on the droplets with particular attention to roles of magnetic attraction, interface properties, and viscosity in the system. This work offers an overall set of tools to understand and predict the behavior of multiple water droplets in an oil-based ferrofluid for lab-on-a-chip applications.
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A current challenge for tissue engineering while restoring the function of diseased or damaged tissue is to customize the tissue according to the target area. Scaffold-free approaches usually yield spheroid shapes with the risk of necrosis at the center due to poor nutrient and oxygen diffusion. Here, we used magnetic forces developed at the cellular scale by miniaturized magnets to create rod-shaped aggregates of stem cells that subsequently matured into a tissue-like structure. However, during the maturation process, the tissue-rods spontaneously bent and coiled into sphere-like structures, triggered by the increasing cell-cell adhesion within the initially non-homogeneous tissue. Optimisation of the intra-tissular magnetic forces successfully hindered the transition, in order to produce stable rod-shaped stem cells aggregates.
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Células-Tronco Mesenquimais/citologia , Engenharia Tecidual/métodos , Fenômenos Biofísicos , Adesão Celular , Agregação Celular , Células Cultivadas , Humanos , Fenômenos Magnéticos , Miniaturização , Esferoides Celulares/citologia , Engenharia Tecidual/instrumentaçãoRESUMO
In recent decades, significant advances have been made in the description and modelling of tissue morphogenesis. By contrast, the initial steps leading to the formation of a tissue structure, through cell-cell adhesion, have so far been described only for small numbers of interacting cells. Here, through the use of remote magnetic forces, we succeeded at creating cell aggregates of half million cells, instantaneously and for several cell types, not only those known to form spheroids. This magnetic compaction gives access to the cell elasticity, found in the range of 800 Pa. The magnetic force can be removed at any time, allowing the cell mass to evolve spontaneously thereafter. The dynamics of contraction of these cell aggregates just after their formation (or, in contrast, their spreading for non-interacting monocyte cells) provides direct information on cell-cell interactions and allows retrieving the adhesion energy, in between 0.05 and 2 mJ m(-2), depending on the cell type tested, and in the case of cohesive aggregates. Thus, we show, by probing a large number of cell types, that cell aggregates behave like complex materials, undergoing a transition from a wet granular to contractile network, and that this transition is controlled by cell-cell interactions.
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Comunicação Celular , Campos Magnéticos , Nanopartículas de Magnetita , Esferoides Celulares/fisiologia , Células 3T3 , Animais , Caderinas/metabolismo , Adesão Celular , Parede Celular/fisiologia , Cães , Células HeLa , Humanos , Células Madin Darby de Rim Canino , CamundongosRESUMO
We report the first observation of axisymmetric solitary waves on the surface of a cylindrical magnetic fluid layer surrounding a current-carrying metallic tube. According to the ratio between the magnetic and capillary forces, both elevation and depression solitary waves are observed with profiles in good agreement with theoretical predictions based on the magnetic analogue of the Korteweg-de Vries equation. We also report the first measurements of the velocity and the dispersion relation of axisymmetric linear waves propagating on the cylindrical ferrofluid layer that are found in good agreement with theoretical predictions.
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Upon drying, colloidal suspensions undergo a phase transformation from a "liquid" to a "gel" state. With further solvent evaporation, tensile stresses develop in the gel, which ultimately leads to fractures. These generally manifest themselves in regular cracking patterns which reflect the physical conditions of the drying process. Here we show experimentally and theoretically how, in the case of a drying droplet of magnetic colloid (ferrofluid), an externally applied magnetic field modifies the stress in the gel and therefore the crack patterns. We find that the analysis of the shape of the cracks allows one to estimate the value of the gel Young's modulus just before the crack nucleation.
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In micro-organisms, as well as in metazoan cells, cellular polarization and directed migration are finely regulated by external stimuli, including mechanical stresses. The mechanisms sustaining the transduction of such external stresses into intracellular biochemical signals remain mainly unknown. Using an external magnetic tip, we generated a magnetic field gradient that allows migration analysis of cells submitted to local low-intensity magnetic forces (50 pN). We applied our system to the amoeba Entamoeba histolytica. Indeed, motility and chemotaxis are key activities that allow this parasite to invade and destroy the human tissues during amoebiasis. The magnetic force was applied either inside the cytoplasm or externally at the rear pole of the amoeba. We observed that the application of an intracellular force did not affect cell polarization and migration, whereas the application of the force at the rear pole of the cell induced a persistent polarization and strongly directional motion, almost directly opposed to the magnetic force. This phenomenon was completely abolished when phosphatidylinositol 3-kinase activity was inhibited by wortmanin. This result demonstrated that the applied mechanical stimulus was transduced and amplified into an intracellular biochemical signal, a process that allows such low-intensity force to strongly modify the migration behavior of the cell.
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Entamoeba histolytica/enzimologia , Entamoeba histolytica/fisiologia , Fosfatidilinositol 3-Quinases/metabolismo , Animais , Fenômenos Biomecânicos , Polaridade Celular , Magnetismo , Mecanotransdução Celular , MovimentoRESUMO
The dynamics of a two-dimensional N-bubble static cloud is investigated and shown to be well described by an N-degree of freedom harmonic oscillator model, at least at low enough frequencies. Eigenmodes and eigenfrequencies are calculated and compared with experimental results obtained with an assembly of bubbles caught up under a net in a water tank. Accordance is found to be excellent in the frequency range of validity of the model, the limits of which are discussed. An interpretation of the low-frequency branch of Foldy's dispersion relation in bubbly liquids is suggested in terms of "bubble waves" in a quasi-incompressible medium.
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We present a non-invasive method to monitor the membrane tension of intracellular organelles using a magnetic field as an external control parameter. By exploiting the spontaneous endocytosis of anionic colloidal ferromagnetic nanoparticles, we obtain endosomes possessing a superparamagnetic lumen in eukaryotic cells. Initially flaccid, the endosomal membrane undulates because of thermal fluctuations, restricted in zero field by the resting tension and the curvature energy of the membrane. When submitted to a uniform magnetic field, the magnetized endosomes elongate along the field, resulting in the flattening of the entropic membrane undulations. The quantification of the endosome deformation for different magnetic fields allows in situ measurement of the resting tension and the bending stiffness of the membrane enclosing the intracellular organelle.
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Membrana Celular/efeitos da radiação , Membrana Celular/ultraestrutura , Campos Eletromagnéticos , Endossomos/efeitos da radiação , Endossomos/ultraestrutura , Compostos Férricos , Fluidez de Membrana/efeitos da radiação , Micromanipulação/métodos , Membrana Celular/fisiologia , Tamanho Celular/fisiologia , Tamanho Celular/efeitos da radiação , Elasticidade , Endossomos/fisiologia , Mecanotransdução Celular/fisiologia , Micromanipulação/instrumentação , Estimulação Física , Estresse MecânicoRESUMO
Anionic iron oxide nanoparticles are efficiently internalized into macrophages where they concentrate within micrometric endosomes, conferring on them a high magnetic susceptibility. The uptake of anionic maghemite nanoparticles by macrophages was quantified by an electron spin resonance (ESR) experiment. MR spin-echo sequences were performed with various TEs and TRs. The contrast enhancement was compared between two types of agarose phantoms with the same equivalent ferrite concentrations but containing either dispersed isolated nanoparticles or magnetically labeled macrophages. It is shown that the intracellular confinement of maghemite nanoparticles within micrometric endosomes results in a significant decrease of the longitudinal relaxivity and a moderate decrease of the transverse relaxivity compared to the relaxivities of the dispersed isolated nanoparticles. As a consequence, the signature of endosomal magnetic labeling consists of a negative contrast on T(1)-weighted images in the whole ferrite concentration range, whereas the presence of extracellular isolated nanoparticles can result in a positive enhancement.
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Meios de Contraste/farmacocinética , Compostos Férricos/farmacocinética , Macrófagos/metabolismo , Imageamento por Ressonância Magnética , Animais , Comunicação Celular , Técnicas de Cultura de Células , Meios de Contraste/química , Espectroscopia de Ressonância de Spin Eletrônica , Compostos Férricos/química , Camundongos , Microscopia Eletrônica de Transmissão e Varredura , Imagens de FantasmasRESUMO
An experimental method based on the rotational dynamics of a magnetic probe is reported to measure the local viscoelasticity of soft materials on microscopic scales. The technique is based on the alignment of dipolar chains of submicrometer magnetic particles in the direction of an applied magnetic field. On one hand, light scattering is used to detect the chains' oscillations over a 0.001-100 Hz frequency range when submitted to an oscillating magnetic field and leads to global microrheological measurements. On the other hand, the chains' rotation toward a permanent magnetic field is observed with a microscope, allowing a local determination of viscoelastic properties on the scale of the chains of particles. We demonstrate the accuracy of both assays with a micellar Maxwellian solution and validate theoretical predictions.
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A new class of superparamagnetic nanoparticles bearing negative surface charges is presented. These anionic nanoparticles show a high affinity for the cell membrane and, as a consequence, are captured by cells with an efficiency three orders of magnitude higher than the widely used dextran-coated iron oxide nanoparticles. The surface coating of anionic particle with albumin strongly reduces the non specific interactions with the plasma membrane as well as the overall cell uptake and at the same time restores the ability to induce specific interactions with targeted cells by the coadsorption on the particle surface of a specific ligand. Kinetics of cellular particle uptake for different cell lines are quantitated using two new complementary assays (Magnetophoresis and Electron Spin Resonance).
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Materiais Biocompatíveis/farmacocinética , Endocitose/fisiologia , Compostos Férricos/farmacocinética , Animais , Materiais Biocompatíveis/síntese química , Transporte Biológico , Birrefringência , Linhagem Celular , Dextranos , Compostos Férricos/síntese química , Células HeLa , Humanos , Imunoglobulina G/metabolismo , Cinética , Macrófagos/metabolismo , Imageamento por Ressonância Magnética , Camundongos , Microscopia Eletrônica , Soroalbumina Bovina/metabolismoRESUMO
The previously developed technique of magnetic rotational microrheology [Phys. Rev. E 67, 011504 (2003)] is proposed to investigate the rheological properties of the cell interior. An endogeneous magnetic probe is obtained inside living cells by labeling intracellular compartments with magnetic nanoparticles, following the endocytosis mechanism, the most general pathway used by eucaryotic cells to internalize substances from an extracellular medium. Primarily adsorbed on the plasma membrane, the magnetic nanoparticles are first internalized within submicronic membrane vesicles (100 nm diameter) to finally concentrate inside endocytotic intracellular compartments (0.6 microm diameter). These magnetic endosomes attract each other and form chains within the living cell when submitted to an external magnetic field. Here we demonstrate that these chains of magnetic endosomes are valuable tools to probe the intracellular dynamics at very local scales. The viscoelasticity of the chain microenvironment is quantified in terms of a viscosity eta and a relaxation time tau by analyzing the rotational dynamics of each tested chain in response to a rotation of the external magnetic field. The viscosity eta governs the long time flow of the medium surrounding the chains and the relaxation time tau reflects the proportion of solidlike versus liquidlike behavior (tau=eta/G, where G is the high-frequency shear modulus). Measurements in HeLa cells show that the cell interior is a highly heterogeneous structure, with regions where chains are embedded inside a dense viscoelastic matrix and other domains where chains are surrounded by a less rigid viscoelastic material. When one compound of the cell cytoskeleton is disrupted (microfilaments or microtubules), the intracellular viscoelasticity becomes less heterogeneous and more fluidlike, in the sense of both a lower viscosity and a lower relaxation time.
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Biofísica/métodos , Biologia Celular , Nanoestruturas/ultraestrutura , Reologia , Adsorção , Calibragem , Membrana Celular/metabolismo , Fenômenos Fisiológicos Celulares , Citoplasma , Citoesqueleto/metabolismo , Endocitose , Células HeLa , Humanos , Microscopia Eletrônica , PressãoRESUMO
Dynamic birefringence in a ferrofluid subjected to crossed bias (constant) and probing (pulse or ac) fields is considered, assuming that the nanoparticles have finite magnetic anisotropy. This is done on the basis of the general Fokker-Planck equation that takes into account both internal magnetic and external mechanical degrees of freedom of the particle. We describe the orientation dynamics in terms of the integral relaxation time of the macroscopic orientation order parameter. To account for an arbitrary relation between the bias (external) and anisotropy (internal) fields, an interpolation expression for the integral relaxation time is proposed and justified. A developed description is used to interpret the measurements of birefringence relaxation in magnetic fluids with nanoparticles of high (cobalt ferrite) and low (maghemite) anisotropy. The proposed theory appears to be in full qualitative agreement with all the experimental data available.
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We present an experimental method allowing a direct measurement of the local rheological behavior of complex fluids. A magnetic probe is inserted into the bulk of the fluid and submitted to a controlled magnetic force or torque, which induces a mechanical perturbation of the fluid. The geometry of the perturbation can be varied using two kinds of probes: a magnetic bead submitted to a homogeneous magnetic force in one direction, and a magnetic needle that can turn inside the material under the effect of an applied magnetic torque. Two complex viscoelastic fluids are investigated. First, a surfactant solution, which has a linear mechanical behavior in the range of the applied stresses, is used to test and validate the experimental methodology. We then use the local probes to investigate a Laponite colloidal suspension, which exhibits nonlinear behavior such as thixotropy, shear thinning, and aging. In this latter fluid, we find an exponential growth of the rheological relaxation time versus the system age, a power-law dependence of the fluid viscosity on the applied stress, and a dynamical yield stress which saturates with the fluid aging time.
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We develop in this paper two methods, based on different physical concepts, to quantify the uptake of magnetic nanoparticles in biological cells. The first one, magnetophoresis, is based on the measurement of the velocity of magnetically labeled cells submitted to a magnetic field gradient. The second one quantitates the particles' electronic spin using an electron paramagnetic resonance experiment. We show a quantitative agreement between both methods for macrophagic cells. The uptake kinetics and uptake capacity are discussed for macrophagic cells and other cell lines.
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Biofísica/métodos , Espectroscopia de Ressonância de Spin Eletrônica/métodos , Compostos Férricos/química , Dendritos/metabolismo , Células Epiteliais/metabolismo , Humanos , Magnetismo , Modelos Estatísticos , Fagocitose , Linfócitos T/metabolismo , Fatores de TempoRESUMO
Small-angle neutron scattering is used to measure the two-dimensional diffraction pattern of a monophasic magnetic colloid, under an applied magnetic field. This dipolar system presents in zero field a fluidlike structure. It is well characterized by an interaction parameter K(0)(T) proportional to the second virial coefficient, which is here positive, expressing a repulsion of characteristic length kappa-10. Under the field a strong anisotropy is observed at the lowest q vectors. The length kappa-10 remains isotropic, but the interaction parameter K(T) becomes anisotropic due to the long-range dipolar interaction. However, the system remains stable, the interaction being repulsive in all directions. Thus we do not observe any chaining of the nanoparticles under magnetic field. On the contrary, the revealed structure of our anisotropic colloid is a lowering of the concentration fluctuations along the field while the fluidlike structure, observed without field, is roughly preserved perpendicularly to the field. It expresses a strong anisotropy of the Brownian motion of the nanoparticles in the solution under applied field.
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We have investigated the relaxation of the magnetically induced birefringence in a suspension of magnetic nanoparticles in order to detect the binding reaction of polyclonal antibodies on the particle surface. The birefringence relaxation is driven by the rotational diffusion of the complex formed by the magnetic nanoparticles bound to the antibody and thus is directly related to the hydrodynamic size of this complex. Birefringence relaxations are well described by stretched exponential laws revealing a polydisperse distribution of hydrodynamic diameters. Comparing the size distribution of samples with different initial ratios of immunoglobuline added per magnetic nanoparticles, we evidence the graft of an antibody on particle and eventually the onset of particles aggregation. Measurements on samples separated in size by gel filtration demonstrate the robustness of our experiment for the determination of size distribution and its modification due to the adsorption of a macromolecule. The immunoglobuline binding assay is performed comparatively for ionic magnetic nanoparticles with different coatings.
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This paper shows how forced Rayleigh scattering can be used as an experimental tool for studying thermodiffusion (Soret effect). The systems investigated are magnetic colloids of different types. A framework including thermodiffusion and dielectrophoresis is described in which the evolutions of temperature and of colloid concentration are clearly distinguished. The framework is then shown to account for experiments on steady-state concentration gratings coupled with transient temperature ones, and the parameters are determined therefrom. Dielectrophoretic forces are found to be negligible. Studying different types of magnetic colloids with various dilution rates shows that the sign of the Soret effect is controlled by the nature of the particle coating made up of electrostatic charges or of surfactant, and that its mechanism is located at the nanoparticle core-solvent interface.
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We propose here a method to modify the membrane tension of phospholipid tubes with an applied magnetic field. The tubes are connected to giant liposomes capping the tubes at both ends. Tubes and liposomes are all filled with a magnetic fluid. The tension of the tube membrane is tuned by the deformation of the ending liposomes under the applied field. We modelize the magnetoliposome deformation and we are then able to describe the tube evolution. At low magnetic fields, the tube remains at equilibrium with a cylindrical shape and a uniform radius. It responds to an increase of membrane tension by a diameter reduction. Above a magnetic-field threshold, the cylindrical shape becomes unstable with respect to a pearling deformation. The tube shape then selected by the system is an unduloid, with a constant mean curvature equal to C 0, the spontaneous curvature of the membrane.
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We explore how a magnetic field breaks the symmetry of an initially spherical giant liposome filled with a magnetic colloid. The condition of rotational symmetry along the field axis leads either to a prolate or to an oblate ellipsoid. We demonstrate that an electrostatic interaction between the nanoparticles and the membrane triggers the shape transition.