Review on application of herbal extracts in biomacromolecules-based nanofibers as wound dressings and skin tissue engineering.

The skin, which covers an area of 2 square meters of an adult human, accounts for about 15 % of the total body weight and is the body's largest organ. It protects internal organs from external physical, chemical, and biological attacks, prevents excess water loss from the body, and plays a role in thermoregulation. The skin is constantly exposed to various damages so that wounds can be acute or chronic. Although wound healing includes hemostasis, inflammatory, proliferation, and remodeling, chronic wounds face different treatment problems due to the prolonged inflammatory phase. Herbal extracts such as Nigella Sativa, curcumin, chamomile, neem, nettle, etc., with varying properties, including antibacterial, antioxidant, anti-inflammatory, antifungal, and anticancer, are used for wound healing. Due to their instability, herbal extracts are loaded in wound dressings to facilitate skin wounds. To promote skin wounds, skin tissue engineering was developed using polymers, bioactive molecules, and biomaterials in wound dressing. Conventional wound dressings, such as bandages, gauzes, and films, can't efficiently respond to wound healing. Adhesion to the wounds can worsen the wound conditions, increase inflammation, and cause pain while removing the scars. Ideal wound dressings have good biocompatibility, moisture retention, appropriate mechanical properties, and non-adherent and proper exudate management. Therefore, by electrospinning for wound healing applications, natural and synthesis polymers are utilized to fabricate nanofibers with high porosity, high surface area, and suitable mechanical and physical properties. This review explains the application of different herbal extracts with different chemical structures in nanofibrous webs used for wound care.

Fabrication of PEGylated SPIONs-Loaded Niosome for Codelivery of Paclitaxel and Trastuzumab for Breast Cancer Treatment: In Vivo Study.

There is a growing appeal for engineering drug delivery systems for controlled and local drug delivery. Conjugation of antibodies on the nanocarriers for targeted chemotherapeutic drugs has always been one of the main techniques. This work aims to develop a polycaprolactone/chitosan electrospun mat incorporated with paclitaxel/Fe3O4-loaded niosomes (SPNs) decorated with trastuzumab (TbNs) for cancer therapy. SPNs and TbNs were analyzed by DLS, zeta potential, scanning electron microscopy, transmission electron microscopy, and Fourier transform infrared spectroscopy. Fabricated mats with distinct concentrations of TbNs were classified into four groups (G0 (0), G1 (1), G2 (2.5), and G3 (5%)) and were studied physicochemically, mechanically, and biologically. Paclitaxel release was also studied for 7 days under an alternative magnetic field (AMF). The optimized mat was nominated for an in vivo study to evaluate its tumor growth inhibition. Based on the results, the TbNs had a spherical core and shell morphology with a smooth surface. The zeta potential and the mean size of TbNs were equal to -14.7 mV and 221 nm. TbNs did not affect the morphology and quality of nanofibers, but in general, the presence of TbNs increased the elastic modulus, water uptake, and degradation. Regarding the release study, AMF showed a significant increase in accelerating paclitaxel release from mats, and most releases belonged to the mat with 5% of TbNs. Results from the in vivo study showed the effective and synergistic effects of AMF on drug release and significant tumor growth inhibition. To summarize, the proposed nanocarrier under AMF can be a good candidate for cancer therapy.

Modeling and optimization of poly(lactic acid)/poly(ℇ-caprolactone)/Nigella sativa extract nanofibers production for skin wounds healing by artificial neural network and response surface methodology models.

Electrospun fibrous scaffolds have great potential for the effective treatment of wounds. Novel blend scaffolds were fabricated from poly(ℇ- caprolactone) (PCL)/poly (lactic acid) (PLA) with Nigella sativa (NS) extract in different concentrations of 10 %, 15 %, 20 %, and 25 % by one nozzle electrospinning. RSM and ANN models were used to determine optimal nanofiber. The results showed that the ANN model had average goodness values of almost 1.992 which was higher than the RSM model with an amount of 1.823. The best sample was determined with the combination of parameters such as PLA/PCL (70:29) concentration, voltage 17 kV, and flow rate 0.2 ml/h in diameter of nanofiber 410 nm by Genetic Algorithm (GA) model with cost value 0.0216 that was lower than cost value (0.0927) of ANN model. The effect of NS extract on nanofibers properties showed that loading high concentrations of NS extract in PLA/PCL polymer solutions caused a decrease in nanofibers diameter, hydrophilicity, and tensile strength. Overall, PLA/PCL/NS 25 % nanofiber was selected as an optimal web with an average diameter of 370 ± 68 nm with a young modulus 5.94 MPa. This scaffold also exhibited the highest antibacterial activity, cell attachment, and cell viability based on the MTT assay.

Investigating the effect of poly (ɛ-caprolactone) nanofibers scaffolds with random, unidirectionally, and radially aligned morphologies on the Fibroblast cell's attachment and growth behavior.

In the last decade, significant progress in tissue engineering, repairing, and replacing organs has been achieved. The design and production of scaffolds for tissue engineering are one of the main areas which have attracted the researcher's interest. In this regard, electrospinning is one of the most popular methods of nanoscale scaffold similar to extracellular matrix production. This paper reports the fabrication of scaffolds consisting of radially aligned PCL nanofibers by utilizing a collector composed of a central point electrode and a peripheral ring electrode. The chemical and physical properties were compared using SEM, FTIR, XRD, and DSC experiments, as well as biological performance using the MTT method and cell morphology with nanofibers with random and unidirectionally morphology. Results of this study showed greater physical and biological properties for radially aligned nanofibers which make them an excellent candidate for wound healing applications due to the guided cell growth on this type of nanofiber.

Oxidized carboxymethyl cellulose/gelatin in situ gelling hydrogel for accelerated diabetic wound healing: Synthesis, characterization, and in vivo investigations.

Diabetic wounds are chronic wounds that are currently affecting many patient's quality of life. These wounds are challenging because of the impaired healing cycle and harsh environment. In this study in situ gelling hydrogels based on oxidized carboxymethyl cellulose (OCMC) and gelatin (Gel) were used to hasten the healing rate due to their ease of application. The suggested system in this work is synthesized from entirely natural renewable biomaterials to not only achieve the best biocompatibility and biodegradability but also to develop a sustainable product. The rheological studies showed that the hydrogel is turned into a gel after about 30 s of the mixing process. Moreover, the hydrogel can absorb about ten times its weight, keeping the wound hydrated. In vitro biological investigations indicated optimal biocompatibility, antibacterial, and antioxidant activity for faster tissue regeneration. This product was tested in vivo on normal rats and diabetic mice models to treat full-thickness incisional wounds. Results showed that the OCMC-Gel hydrogel is able to hasten the healing rate in both non-diabetic and diabetic wounds. Pathological examinations of the regenerated skin tissue revealed that the OCMC-Gel treated groups developed much more than the control group.

A conductometric enzymatic methanol sensor based on polystyrene - PAMAM dendritic polymer electrospun nanofibers.

Methanol (MeOH) is a solvent and cleaning agent used in industry, but it is poisonous when ingested. The recommended release threshold for MeOH vapor is 200 ppm. We present a novel sensitive micro-conductometric MeOH biosensor created by grafting alcohol oxidase (AOX) onto electrospun polystyrene-poly(amidoamine) dendritic polymer blend nanofibers (PS-PAMAM-ESNFs) on interdigitated electrodes (IDEs). The analytical performance of the MeOH microsensor was evaluated using gaseous MeOH, ethanol, and acetone samples collected from the headspace above aqueous solution with known concentration. The sensor's response time (tRes) fluctuates from 13 s to 35 s from lower to higher concentrations. The conductometric sensor has a sensitivity of 150.53 µS.cm-1 (v/v) for MeOH and a detection limit of 100 ppm in the gas phase. The MeOH sensor is 7.3 times less sensitive to ethanol and 136.8 times less sensitive to acetone. The sensor was verified for detecting MeOH in commercial rubbing alcohol samples.

Fabrication and investigating in vivo wound healing property of coconut oil loaded nanofiber/hydrogel hybrid scaffold.

Obtaining a sustainable drug delivery system is a challenging issue in biomedical science. This became even more important in the wound regeneration process due to its long treatment process. In this study, the calcium alginate (CaAlg) hydrogel is coated on the surface of polycaprolactone (PCL)/gelatin (Gel) nanofibers containing coconut oil (CO) using the impregnation method. The physical, chemical, and morphological properties of produced samples are investigated using different characterization techniques to verify the influence of hydrogel. Water contact angle, swelling ratio, and water vapor permeability measurements are used to evaluate the effect of hydrogel on the hydrophilicity of the proposed system. The cell viability test showed that the nanocomposite hydrogel is biocompatible and could improve wound healing. According to drug release studies, hydrogel addition to the nanofiber system plays an essential role in controlling CO release rate in the first 250 h. In vivo studies also indicated faster skin regeneration.

Fabrication of multifunctional mucoadhesive buccal patch for drug delivery applications.

Mucoadhesive buccal patch is a promising dosage form for a successful oral drug delivery, which provides unique advantages for various applications such as treatment of periodontal disease and postdental surgery disorders. The aim of this study is to synthesize a novel multifunctional mucoadhesive buccal patch in a multilayer reservoir design for therapeutic applications. The patches were fabricated through simultaneous electrospinning of chitosan/poly(vinylalcohol) (PVA)/ibuprofen and electrospraying of phenylalanine amino acid nanotubes (PhNTs) containing metronidazole into the electrospun mats through a layer-by-layer process. An electrospun poly(caprolactone) (PCL) was used as an impermeable backing layer to protect the mucoadhesive component from tongue movement and drug loss. Buccal patches were characterized using scanning electron microscopy (SEM) and field emission scanning electron microscopy (FESEM) and also evaluated in terms of physicomechanical parameters such as pH, weight, thickness, tensile strength, folding endurance, and mucoadhesive properties. The swelling index of the patches was examined with respect to the PVA/chitosan ratio. The effect of genipin addition to the electrospinning solution was also studied on mucoadhesive and swelling properties. The cell viability of buccal patches was assessed by methylthiazolydiphenyl-tetrazolium bromide test on L929 fibroblast cell line. The patch with an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g) indicated an ideal hemostatic activity along with antibacterial properties against Streptococcus mutans bacteria. The synthesized multifunctional mucoadhesive patch with a novel composition and design has a great potential for oral therapeutic applications.

Synthesis and Characterization of Exopolysaccharide Encapsulated PCL/Gelatin Skin Substitute for Full-Thickness Wound Regeneration.

Loss of skin integrity can lead to serious problems and even death. In this study, for the first time, the effect of exopolysaccharide (EPS) produced by cold-adapted yeast R. mucilaginosa sp. GUMS16 on a full-thickness wound in rats was evaluated. The GUMS16 strain's EPS was precipitated by adding cold ethanol and then lyophilized. Afterward, the EPS with polycaprolactone (PCL) and gelatin was fabricated into nanofibers with two single-needle and double-needle procedures. The rats' full-thickness wounds were treated with nanofibers and Hematoxylin and eosin (H&E) and Masson's Trichrome staining was done for studying the wound healing in rats. Obtained results from SEM, DLS, FTIR, and TGA showed that EPS has a carbohydrate chemical structure with an average diameter of 40 nm. Cell viability assessments showed that the 2% EPS loaded sample exhibits the highest cell activity. Moreover, in vivo implantation of nanofiber webs on the full-thickness wound on rat models displayed a faster healing rate when EPS was loaded into a nanofiber. These results suggest that the produced EPS can be used for skin tissue engineering applications.

Cellulose nanocrystal effect on crystallization kinetics and biological properties of electrospun polycaprolactone.

Mechanical properties of tissue engineering nanofibrous scaffolds are of importance because they not only determine their ease of application, but also influence the environment for cell growth and proliferation. Cellulose nanocrystals (CNCs) are natural renewable nanoparticles that have been widely used for manipulating nanofibers' mechanical properties. In this article, cellulose nanoparticles were incorporated into poly(caprolactone) (PCL) solution, and composite nanofibers were produced. Ozawa-Flynn-Wall (OFW) methodology and X-ray diffraction were used to investigate the effect of CNC incorporation on PCL crystalline structure and its biological properties. Results showed that CNC incorporation up to 1% increases the crystallization activation energy and reduces the crystal volume, while these factors remain constant above this critical concentration. MTT assay and microscopic images of seeded cells on the nanofiber scaffolds indicated increased cell growth on the samples containing CNC. This behavior could be attributed to their greater hydrophilicity, which was confirmed using parallel exponential kinetics (PEK) model fitting to results obtained from dynamic vapor sorption (DVS) studies. Superior performance of CNC containing samples was also confirmed by in vivo implantation on full-thickness wounds. The wound area faded away more rapidly in these samples. H&E and Masson's trichrome staining showed better regeneration and more developed tissues in wounds treated with PCL-CNC1% nanofibers.

Cinnamon extract loaded electrospun chitosan/gelatin membrane with antibacterial activity.

This study develops chitosan/gelatin nanofiber membranes with sustained release capacity to prevent infection by delivering cinnamon extract (CE) in the implanted site. The effects of the incorporation of CE content (2-6%) on the properties of the nanofibers were evaluated. Morphological studies using SEM indicated that loading the extract did not affect the average diameter of nanofiber mats, which remained around 140-170 nm. TGA and FTIR spectroscopy results confirmed successful CE loading. Furthermore, the results showed that incorporating extract into the nanofibers enhanced their degradation behavior, antibacterial activity, and biocompatibility. Cultured cells attached to and proliferate on the nanofiber membrane with high cell viability capacity until the CE content reached 4%. The extract release profile consisted of a burst release in the first 6 h, followed by a controlled release in the next 138 h. Therefore, CE loaded chitosan/gelatin nanofiber is an excellent construct for biomedical applications.

Biomimetic double-sided polypropylene mesh modified by DOPA and ofloxacin loaded carboxyethyl chitosan/polyvinyl alcohol-polycaprolactone nanofibers for potential hernia repair applications.

Polypropylene (PP) meshes are the most widely used as hernioplasty prostheses. As far as hernia repair is concerned, bacterial contamination and tissue adhesion would be the clinical issues. Moreover, an optimal mesh should assist the healing process of hernia defect and avoid undesired prosthesis displacements. In this present study, the commercial hernia mesh was modified to solve the mentioned problems. Accordingly, a new bi-functional PP mesh with anti-adhesion and antibacterial properties on the front and adhesion properties (reduce undesired displacements) on the backside was prepared. The backside of PP mesh was coated with polycaprolactone (PCL) nanofibers modified by mussel-inspired L-3,4-dihydroxyphenylalanine (L-DOPA) bioadhesive. The front side was composed of two different nanofibrous mats, including hybrid and two-layered mats with different antibacterial properties, drug release, and biodegradation behavior, which were based on PCL nanofibers and biomacromolecule carboxyethyl-chitosan (CECS)/polyvinyl alcohol (PVA) nanofibers containing different ofloxacin amounts. The anti-adhesion, antibacterial, and biocompatibility studies were done through in-vitro experiments. The results revealed that DOPA coated PCL/PP/hybrid meshes containing ofloxacin below 20 wt% possessed proper cell viability, AdMSCs adhesion prevention, and excellent antibacterial efficiency. Moreover, DOPA modifications not only enhanced the surface properties of the PP mesh but also improved cell adhesion, spreading, and proliferation.

Modeling and optimization of Photocatalytic Decolorization of binary dye solution using graphite electrode modified with Graphene oxide and TiO2.

In this paper, the experimental design methodology was employed for modeling and optimizing the operational parameters of the photocatalytic degradation of a binary dye solution using a fixed photocatalytic compound. The compound used was modified graphite electrode (GE) with graphene oxide (GO) on which TiO2 nanoparticles were immobilized. GO nanoparticle was deposited on graphite electrode (GO-GE) using electrochemical approach. TiO2 nanoparticles were immobilized on GO-GE by solvent evaporation method. A binary solution containing mixture of methylene blue (MB) and acid red 14 (AR14) was chosen as dye model. The degradation intermediates were detected and analyzed using gas chromatography. Effect of different factors on the photocatalytic decolorization efficiency was investigated and optimized using response surface methodology (RSM). The obtained results indicated that the prepared TiO2-GO-CE can decolorize MB with high efficiency (93.43%) at pH 11, dye concentration of 10 mg/L and 0.04 g of immobilized TiO2 on the GO fabricated plates after 120 min of photocatalytic process. It was demonstrated that by modifying GE with GO the stability of the electrode was remarkably enhanced. The ANOVA results (R2 = 0.97 and P value <0.0001 for MB, R2 = 0.96 and P value <0.0001 for AR14) and numerical optimization showed that it is possible to make good prediction on decoloration behavior and save time and energy with less number of experiments using design of experiments (DoE) like the RSM. Graphical abstract Wastewater treatment processWastewater treatment process.

Fabricating alginate/poly(caprolactone) nanofibers with enhanced bio-mechanical properties via cellulose nanocrystal incorporation.

In this research, cellulose nanocrystal (CNC) was synthesized from cotton waste using controlled hydrolysis against 64 % (w/w) sulfuric acid solution. The produced nanoparticles were then characterized using FTIR, XRD, TGA, and DLS analyses. Biaxial electrospinning technique was used to produce CNC incorporated PCL-PVA/NaAlg nanofibers. The sodium alginate portion was then crosslinked via submerging the samples in calcium chloride aqueous solution. The CNC incorporated and crosslinked sample was characterized using SEM, FTIR, and TGA techniques. Results confirmed the presence of CNC nanoparticles and alginate crosslinking reaction. Mechanical studies showed that CNC incorporation increases the tensile modulus by 65 %. Also, the crosslinked samples exhibited an increase in elongation at break. Water contact angle studies suggested that CNC incorporation and crosslinking improves nanofiber hydrophilicity. Cell viability of more than 90 % was observed in CNC incorporated PCL-CaAlg nanofibers. Also, SEM images of cells on nanofiber scaffolds showed better cell growth and attachment in PCL-CaAlg-CNC samples.

Multilayer nanofibrous patch comprising chamomile loaded carboxyethyl chitosan/poly(vinyl alcohol) and polycaprolactone as a potential wound dressing.

Electrospun multilayer nanofibrous patches with a new design were developed using poly(ε-caprolactone) (PCL) and chamomile loaded carboxyethyl chitosan (CECS) and polyvinyl alcohol (PVA) in which chamomile extract was used as an antioxidant/antibacterial agent. To prepare an aqueous solution (water as solvent) from chitosan and PVA along with the herbal extract, chitosan was modified to CECS by Michael reaction and proved by 1H NMR and FTIR. Multilayer patches composed of a hydrophilic chamomile loaded CECS/PVA nanofibrous layer to be in contact with the wound and a hydrophobic PCL nanofibrous layer to provide the strength were electrospun. Hybrid nanofibers made of PCL and chamomile/CECS/PVA were electrospun as cohesion promoter between the hydrophilic and hydrophobic layers due to their different chemical nature and weak cohesion. SEM showed continuous, smooth, and bead-free nanofibers with excellent compatibility between polymers and chamomile. The mats exhibited satisfactory tensile strength (8.2-16.03 MPa), and antioxidant characteristics (6.60-38.01%). Furthermore, 15, 20, and 30 wt% chamomile loaded mats possessed high antibacterial efficiency, which enhanced with increasing chamomile content. The results demonstrated that chamomile sustained-release significantly controlled by Fickian-Diffusion mechanism. MTT assay revealed proper cell viability for all mats except one contained 30 wt% chamomile.

Smart electrospun nanofibers containing PCL/gelatin/graphene oxide for application in nerve tissue engineering.

Currently graphene-doped electrospun scaffolds have been a matter of great interest to be exploited in biomedical fields such as tissue engineering and drug delivery applications. The main objective of this paper is to evaluate the effect of graphene on biological properties of PCL/gelatin nanofibrous mats. SEM analysis was conducted to investigate the morphology of the electrospun nanofibers. The in-vitro cellular proliferation of PC12 cells on nanofibrous web was also investigated. Electrospun PCL/gelatin/graphene nanofibrous mats exhibited 99% antibacterial properties against gram-positive and gram-negative bacteria. Drug release studies indicated that the π-π stacking interaction between TCH and graphene has led to the far better controlled release of TCH from electrospun PCL/gelatin/graphene compared to PCL/gelatin nanofibrous scaffolds. These superior properties along with an improvement in hydrophilicity and biodegradation features has made the nanofibers a promising candidate to be used as electrically conductive scaffolds in neural tissue engineering as well as controlled drug delivery.

Drug release and biodegradability of electrospun cellulose nanocrystal reinforced polycaprolactone.

In this paper, high molecular weight cellulose was used as the starting material for the synthesis of cellulose nanocrystal (CNC). Different analysis techniques such as FTIR, XRD, TGA, DLS, and AFM were used to characterize CNC synthesis. The synthesized CNC was incorporated in polycaprolactone solution and nanofibers were prepared under different conditions. Production conditions were optimized based on the diameter of nanofibers using response surface methodology (RSM). Based on our results, the optimal condition is electrospinning of 16% PCL polymer solution at 17 kV and a 0.9 ml/h feed rate, which yields nanofibers with a diameter of 233 nm. The effects of CNC content on morphological, mechanical and thermal properties were investigated. Results also showed that CNC incorporation in PCL nanofibers enhances biodegradability. SEM, DSC, tensile, and biodegradability results showed that the nanofibers prepared from PCL solution containing 1% CNC have optimal mechanical and degradation behaviors. We also studied and modeled release of tetracycline from nanofiber mats, based on the assumption of rate limiting diffusion from the nanofibers, with a fraction of release delayed by drug sequestration. Results showed that the final drug release is decreased in CNC-incorporated nanofibers.

Electrical stimulation of somatic human stem cells mediated by composite containing conductive nanofibers for ligament regeneration.

One of the advances in the field of biomedical nanotechnology, is conductive nanofiber fabrication and the discovery of its applications. Biocompatible flexible nanofibers that have a good biocompatibility, mechanical properties and morphology. Poly (3, 4-ethylene dioxythiophene) (PEDOT) is a conductive polymer that has recently been used in medical applications. In this study, the electrospinning technique and vapor phase polymerization combination method with freeze drying was used to produce Silk fibroin/PEDOT/Chitosan nanocomposite scaffold. The aim of our study was to develop a ligament construct of PEDOT/Silk bilayer nanofibrous scaffold, to mimic the aligned collagen fiber bundles and Chitosan sponge coating was done on these fibrous scaffolds, to mimic the glycosaminoglycans of ECM sheath. The developed constructs were characterized. The unrestricted somatic human stem cells (USSC), were cultured on the scaffold. Then, the effect of applying DC electric pulses to cells cultured on polymer was assessed. Cellular function was actively exhibited in scaffold with electrical induction, as evident by the high expression of collagen I, collagen III, decorin, biglycan and aggrecan genes. Novel scaffold plus electrical stimulation shows facilitating cell seeding and promoting cell proliferation, differentiation. This composites can be used in this new field for stem cells differentiation to target tissues.

Fabrication and characterization of PVA/Gum tragacanth/PCL hybrid nanofibrous scaffolds for skin substitutes.

In this work three dimensional biodegradable nanofiberous scaffolds containing poly(ε-caprolactone) (PCL), poly(vinyl alcohol) (PVA) and gum tragacanth (GT) were successfully fabricated through two nozzles electrospinning process. For this purpose, PVA/GT blend (Blend: B) solution (60:40wt%) was injected from one syringe and poly(ε-caprolactone) solution from the other one. Presence of PVA and PCL in the formulation improved the electrospinning process of GT solution and mechanical properties of the fabricated nanofibers. Scanning electron microscopy (SEM) results showed uniform PVA/GT-PCL blend-hybrid (Blend-Hybrid: B-H) nanofibers with the diameter ranging about 132±27nm. Hybrid nanofibers were evaluated by Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC) tests. The antibacterial activities of the PVA/GT-PCL (B-H) nanofibers were conducted against Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus and results indicated that the hybrid nanofibers were 95.19% antibacterial against S. aureus bacterium. NIH 3T3 fibroblast cells growth and MTT assay were carried out on the scaffolds. Hydrophilicity nature, favorable mechanical properties of the fabricated hybrid nanofibers, along with their structure in biological media, biocompatibility, as well as antibacterial property indicate scaffolds prepared are suitable for tissue engineering.

Antibacterial performance and in vivo diabetic wound healing of curcumin loaded gum tragacanth/poly(ε-caprolactone) electrospun nanofibers.

In this study we describe the potential of electrospun curcumin-loaded poly(ε-caprolactone) (PCL)/gum tragacanth (GT) (PCL/GT/Cur) nanofibers for wound healing in diabetic rats. These scaffolds with antibacterial property against methicillin resistant Staphylococcus aureus as gram positive bacteria and extended spectrum ß lactamase as gram negative bacteria were applied in two forms of acellular and cell-seeded for assessing their capability in healing full thickness wound on the dorsum of rats. After 15days, pathological study showed that the application of GT/PCL/Cur nanofibers caused markedly fast wound closure with well-formed granulation tissue dominated by fibroblast proliferation, collagen deposition, complete early regenerated epithelial layer and formation of sweat glands and hair follicles. No such appendage formation was observed in the untreated controls during this duration. Masson's trichrome staining confirmed the increased presence of collagen in the dermis of the nanofiber treated wounds on day 5 and 15, while the control wounds were largely devoid of collagen on day 5 and exhibited less collagen amount on day 15. Quantification analysis of scaffolds on day 5 confirmed that, tissue engineered scaffolds with increased amount of angiogenesis number, granulation tissue area (µ(2)), fibroblast number, and decreased epithelial gap (µ) can be more effective compared to GT/PCL/Cur nanofibers.