Synergistic adsorption of methylene blue with carrageenan/hydrochar-derived activated carbon hydrogel composites: Insights and optimization strategies.

The study explores the use of hydrochar-derived activated carbon (AC) to improve the adsorption capacity and mechanical properties of carrageenan (CAR) hydrogel beads. Four distinct samples, with carrageenan to activated carbon ratios of 1:0 (CAR), 2:1 (CAC2), 4:1 (CAC4), and 10:1 (CAC10), were prepared. These polymeric beads underwent comprehensive evaluation for their methylene blue (MB) adsorption capacity, gel content (GC), and swelling ratio (SR). Increasing activated carbon content up to 50 % of carrageenan mass significantly enhanced GC and SR by 20.57 % and 429.24 %, respectively. Various analytical techniques were employed to characterize the composites, including FTIR, XRD, Raman Spectroscopy, BET, SEM, and EDS-Mapping. Batch adsorption tests investigated the effects of pH, contact time, dye concentration, and temperature on MB adsorption. Maximum adsorption capacities for CAR, CAC10, CAC4, and CAC2 were 475.48, 558.54, 635.93, and 552.35 mg/g, respectively, under optimal conditions. Kinetic models (Elovich and pseudo-second-order) and isotherm models (Temkin for CAR and Freundlich for CAC10, CAC4, and CAC2) fitted well with the experimental data. Thermodynamic analysis showed spontaneous, exothermic MB adsorption. Primary mechanisms include electrostatic attraction, hydrogen bonding, n-π, and π-π stacking. The study highlights enhanced adsorption capacity of carrageenan hydrogel via carrageenan/activated carbon composites, providing cost-effective wastewater treatment.

Innovations in hydrogel-based manufacturing: A comprehensive review of direct ink writing technique for biomedical applications.

Direct ink writing (DIW) stands as a pioneering additive manufacturing technique that holds transformative potential in the field of hydrogel fabrication. This innovative approach allows for the precise deposition of hydrogel inks layer by layer, creating complex three-dimensional structures with tailored shapes, sizes, and functionalities. By harnessing the versatility of hydrogels, DIW opens up possibilities for applications spanning from tissue engineering to soft robotics and wearable devices. This comprehensive review investigates DIW as applied to hydrogels and its multifaceted applications. The paper introduces a diverse range of printing techniques while providing a thorough exploration of DIW for hydrogel-based printing. The investigation aims to explain the progress made, challenges faced, and potential trajectories that lie ahead for DIW in hydrogel-based manufacturing. The fundamental principles underlying DIW are carefully examined, specifically focusing on rheological attributes and printing parameters, prompting a comprehensive survey of the wide variety of hydrogel materials. These encompass both natural and synthetic variations, all of which can be effectively harnessed for this purpose. Furthermore, the review explores the latest applications of DIW for hydrogels in biomedical areas, with a primary focus on tissue engineering, wound dressing, and drug delivery systems. The document not only consolidates the existing state of DIW within the context of hydrogel-based manufacturing but also charts potential avenues for further research and innovative breakthroughs.

Lignin beyond the status quo: recent and emerging composite applications.

The demand for biodegradable materials across various industries has recently surged due to environmental concerns and the need for the adoption of renewable materials. In this context, lignin has emerged as a promising alternative, garnering significant attention as a biogenic resource that endows functional properties. This is primarily ascribed to its remarkable origin and structure that explains lignin's capacity to bind other molecules, reinforce composites, act as an antioxidant, and endow antimicrobial effects. This review summarizes recent advances in lignin-based composites, with particular emphasis on innovative methods for modifying lignin into micro and nanostructures and evaluating their functional contribution. Indeed, lignin-based composites can be tailored to have superior physicomechanical characteristics, biodegradability, and surface properties, thereby making them suitable for applications beyond the typical, for instance, in ecofriendly adhesives and advanced barrier technologies. Herein, we provide a comprehensive overview of the latest progress in the field of lignin utilization in emerging composite materials.

Heat-Induced Actuator Fibers: Starch-Containing Biopolyamide Composites for Functional Textiles.

This study introduces the development of a thermally responsive shape-morphing fabric using low-melting-point polyamide shape memory actuators. To facilitate the blending of biomaterials, we report the synthesis and characterization of a biopolyamide with a relatively low melting point. Additionally, we present a straightforward and solvent-free method for the compatibilization of starch particles with the synthesized biopolyamide, aiming to enhance the sustainability of polyamide and customize the actuation temperature. Subsequently, homogeneous dispersion of up to 70 wt % compatibilized starch particles into the matrix is achieved. The resulting composites exhibit excellent mechanical properties comparable to those reported for soft and tough materials, making them well suited for textile integration. Furthermore, cyclic thermomechanical tests were conducted to evaluate the shape memory and shape recovery of both plain polyamide and composites. The results confirmed their remarkable shape recovery properties. To demonstrate the potential application of biocomposites in textiles, a heat-responsive fabric was created using thermoresponsive shape memory polymer actuators composed of a biocomposite containing 50 wt % compatibilized starch. This fabric demonstrates the ability to repeatedly undergo significant heat-induced deformations by opening and closing pores, thereby exposing hidden functionalities through heat stimulation. This innovative approach provides a convenient pathway for designing heat-responsive textiles, adding value to state-of-the-art smart textiles.

Strontium-Substituted Nanohydroxyapatite-Incorporated Poly(lactic acid) Composites for Orthopedic Applications: Bioactive, Machinable, and High-Strength Properties.

Traditional metal-alloy bone fixation devices provide structural support for bone repair but have limitations in actively promoting bone healing and often require additional surgeries for implant removal. In this study, we focused on addressing these challenges by fabricating biodegradable composites using poly(lactic acid) (PLA) and strontium-substituted nanohydroxyapatite (SrHAP) via melt compounding and injection molding. Various percentages of SrHAP (5, 10, 20, and 30% w/w) were incorporated into the PLA matrix. We systematically investigated the structural, morphological, thermal, mechanical, rheological, and dynamic mechanical properties of the prepared composites. Notably, the tensile modulus, a critical parameter for orthopedic implants, significantly improved from 2.77 GPa in pristine PLA to 3.73 GPa in the composite containing 10% w/w SrHAP. The incorporation of SrHAP (10% w/w) into the PLA matrix led to an increased storage modulus, indicating a uniform dispersion of SrHAP within the PLA and good compatibility between the polymer and nanoparticles. Moreover, we successfully fabricated screws using PLA composites with 10% (w/w) SrHAP, demonstrating their formability at room temperature and radiopacity when observed under X-ray microtomography (micro-CT). Furthermore, the water contact angle decreased from 93 ± 2° for pristine PLA to 75 ± 3° for the composite containing SrHAP, indicating better surface wettability. To assess the biological behavior of the composites, we conducted in vitro cell-material tests, which confirmed their osteoconductive and osteoinductive properties. These findings highlight the potential of our developed PLA/SrHAP10 (10% w/w) composites as machinable implant materials for orthopedic applications. In conclusion, our study presents the fabrication and comprehensive characterization of biodegradable composites comprising PLA and strontium-substituted nanohydroxyapatite (SrHAP). These composites exhibit improved mechanical properties, formability, and radiopacity while also demonstrating desirable biological behavior. Our results suggest that these PLA/SrHAP10 composites hold promise as machinable implant materials for orthopedic applications.

High-resolution 3D printing of xanthan gum/nanocellulose bio-inks.

The current study provides a comprehensive rheology study and a survey on direct ink writing of xanthan gum/cellulose nanocrystal (XG/CNC) bio-inks for developing 3D geometries that mimic soft tissue engineering scaffolds' physical and mechanical properties. The presence of CNC was found to be a critical prerequisite for the printability of XG bio-inks; accordingly, the hybrid XG/CNC bio-inks revealed the excellent viscoelastic properties that enabled precise control of hydrogel shaping and printing of lattice structures composed of up to eleven layers with high fidelity and fair resolution without any deformation after printing. The lyophilized 3D scaffolds presented a porous structure with open and interconnected pores and a porosity higher than 70%, vital features for tissue engineering scaffolds. Moreover, they showed a relatively high swelling of approximately 11 g/g, facilitating oxygen and nutrient exchange. Furthermore, the elastic and compressive moduli of the scaffolds that enhanced significantly upon increasing CNC content were in the range of a few kPa, similar to soft tissues. Finally, no significant cell cytotoxicity was observed against human liver cancer cells (HepG2), highlighting the potential of these developed 3D printed scaffolds for soft tissue engineering applications.

3D printing and properties of cellulose nanofibrils-reinforced quince seed mucilage bio-inks.

Plant-based hydrogels have attracted great attention in biomedical fields since they are biocompatible and based on natural, sustainable, cost-effective, and widely accessible sources. Here, we introduced new viscoelastic bio-inks composed of quince seed mucilage and cellulose nanofibrils (QSM/CNF) easily extruded into 3D lattice structures through direct ink writing in ambient conditions. The QSM/CNF inks enabled precise control on printing fidelity where CNF endowed objects with shape stability after freeze-drying and with suitable porosity, water uptake capacity, and mechanical strength. The compressive and elastic moduli of samples produced at the highest CNF content were both increased by ~100% (from 5.1 ± 0.2 kPa and 32 ± 1 kPa to 10.7 ± 0.5 and 64 ± 2 kPa, respectively). These values ideally matched those reported for soft tissues; accordingly, the cell compatibility of the printed samples was evaluated against HepG2 cells (human liver cancer). The results confirmed the 3D hydrogels as being non-cytotoxic and suitable to support attachment, survival, and proliferation of the cells. All in all, the newly developed inks allowed sustainable 3D bio-hydrogels fitting the requirements as scaffolds for soft tissue engineering.

Ascorbic acid-loaded polyvinyl alcohol/cellulose nanofibril hydrogels as precursors for 3D printed materials.

We proposed a simple method to process hydrogels containing polyvinyl alcohol and cellulose nanofibrils (PVA/CNF) to prepare volumetric architectures by direct ink writing (DIW). The presence of CNF in the aqueous PVA suspensions conferred rheology profiles that were suitable for extrusion and solidification in pre-designed shapes. The viscoelastic behavior of the hybrid inks enabled precise control on processability and shape retention, for instance, as demonstrated in multilayered lattice structures of high fidelity. After lyophilization, the obtained 3D-printed hydrogels presented a very high porosity, with open and interconnected pores, allowing a high-water uptake capacity (up to 1600%). The mechanical strength of the composite 3D-printed materials matched those of soft tissues, opening opportunities for skin applications. As such, drug-loaded samples revealed a controlled and efficient delivery of an antioxidant (ascorbic acid) in PBS buffer media at 23 °C (~80% for 8 h). Altogether, PVA/CNF hydrogels were introduced as suitable precursors of 3D-lattice geometries with excellent physical and mechanical characteristics.

Fabrication and characterisation of a wound dressing composed of polyvinyl alcohol and quince seed mucilage.

OBJECTIVE: Providing a suitable environment to improve the healing process is the main target of wound dressing that also protects the wound from additional harms. In the present study, fabrication and characterisation of a new kind of electrospun wound dressing composed of polyvinyl alcohol (PVA) and quince seed mucilage (QSM) is reported. METHOD: QSM was extracted from quince seeds, purified, freeze-dried and used to produce aqueous solutions containing different amounts of PVA and QSM. The wound dressings were fabricated via the electrospinning method and their characteristics were investigated with scanning electron microscope (SEM) images, Fourier transform infrared (FTIR) spectra, tensile and swelling test, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) cytotoxicity assay against fibroblast cells. RESULTS: SEM images confirmed that proper, uniform, non-oriented nanofibres with an average diameter in the range of 60-240nm, depending on the QSM content had been fabricated. The tensile test showed that with increasing QSM content, the tensile strength of fibre increased while elongation at break was decreased, which was consistent with SEM images where the diameter of samples decreased by increasing QSM content. MTT assay showed significant biocompatibility against fibroblast cells; however, it was increased by increased QSM proportion. In addition, SEM images supported the proper adhesion of fibroblast cells on the sample one day after culturing. CONCLUSION: Overall, the findings of the current study support the potential of PVA/QSM nanofibres as a proper candidate for biomedical applications, especially as a wound dressing.

Alginate/cartilage extracellular matrix-based injectable interpenetrating polymer network hydrogel for cartilage tissue engineering.

In the present study, alginate/cartilage extracellular matrix (ECM)-based injectable hydrogel was developed incorporated with silk fibroin nanofibers (SFN) for cartilage tissue engineering. The in situ forming hydrogels were composed of different ionic crosslinked alginate concentrations with 1% w/v enzymatically crosslinked phenolized cartilage ECM, resulting in an interpenetrating polymer network (IPN). The response surface methodology (RSM) approach was applied to optimize IPN hydrogel's mechanical properties by varying alginate and SFN concentrations. The results demonstrated that upon increasing the alginate concentration, the compression modulus improved. The SFN concentration was optimized to reach a desired mechanical stiffness. Accordingly, the concentrations of alginate and SFN to have an optimum compression modulus in the hydrogel were found to be 1.685 and 1.724% w/v, respectively. The gelation time was found to be about 10 s for all the samples. Scanning electron microscope (SEM) images showed homogeneous dispersion of the SFN in the hydrogel, mimicking the natural cartilage environment. Furthermore, water uptake capacity, degradation rate, cell cytotoxicity, and glycosaminoglycan and collagen II secretions were determined for the optimum hydrogel to support its potential as an injectable scaffold for articular cartilage defects.

Direct ink writing of aloe vera/cellulose nanofibrils bio-hydrogels.

Direct-ink-writing (DIW) of hydrogels has become an attractive research area due to its capability to fabricate intricate, complex, and highly customizable structures at ambient conditions for various applications, including biomedical purposes. In the current study, cellulose nanofibrils reinforced aloe vera bio-hydrogels were utilized to develop 3D geometries through the DIW technique. The hydrogels revealed excellent viscoelastic properties enabled extruding thin filaments through a nozzle with a diameter of 630 µm. Accordingly, the lattice structures were printed precisely with a suitable resolution. The 3D-printed structures demonstrated significant wet stability due to the high aspect ratio of the nano- and microfibrils cellulose, reinforced the hydrogels, and protected the shape from extensive shrinkage upon drying. Furthermore, all printed samples had a porosity higher than 80% and a high-water uptake capacity of up to 46 g/g. Altogether, these fully bio-based, porous, and wet stable 3D structures might have an opportunity in biomedical fields.

Design and fabrication of conductive nanofibrous scaffolds for neural tissue engineering: Process modeling via response surface methodology.

Peripheral nervous system in contrary to central one has the potential for regeneration, but its regrowth requires proper environmental conditions and supporting growth factors. The aim of this study is to design and fabricate a conductive polyaniline/graphene nanoparticles incorporated gelatin nanofibrous scaffolds suitable for peripheral nervous system regeneration. The scaffolds were fabricated with electrospinning and the fabrication process was designed with Design-Expert software via response surface methodology. The effect of process parameters including applied voltage (kV), syringe pump flow rate (cm3/h), and PAG concentration (wt%), on the scaffold conductivity, nanofibers diameter, and cell viability were investigated. The obtained results showed that the scaffold conductivity and cell viability are affected by polyaniline/graphene concentration while nanofiber diameter is more affected by the applied voltage and syringe pump flow rate. Optimum scaffold with maximum conductivity (0.031 ± 0.0013 S/cm) and cell compatibility and suitable diameter were electrospun according to the software introduced values for the process parameters (voltage of 13 kV, flow rate of 0.1 cm3/h, and PAG wt.% of 1.3) and its morphology, cell compatibility, and biodegradability were further investigated, which showed its potential for applying in peripheral nervous system injury regeneration.

Investigation the effect of graphene oxide and gelatin/starch weight ratio on the properties of starch/gelatin/GO nanocomposite films: The RSM study.

In this study, we reported the design and fabrication of starch/gelatin/graphene oxide biodegradable nanocomposite films with proper potential for food packaging applications. The experiments were designed using Design Expert software via RSM by defining gelatin/starch weight ratio (G/S wt. ratio) and graphene oxide (GO) wt.% as process parameters affecting on the properties of the prepared films. The optimization was done and various properties of the optimum film were investigated. The XRD and SEM images confirmed that GO layers could intercalate in the starch/gelatin matrix and proper dispersion of nanofillers in the matrix obtained via solution casting method. The mechanical strength, thermal stability, surface hydrophilicity, and water vapor permeability were improved in the present of GO which could be related to reinforcing effect and proper barrier property of GO. Degradation of the prepared films under the soil was investigated during 6 weeks and the results indicated at least 30% weight loss happened throughout the test duration. Overall, the obtained results corroborate that the starch/gelatin/GO compounding can be a good candidate for fabricating of biodegradable nanocomposite films for food packaging applications.

Fabrication of porous gelatin-chitosan microcarriers and modeling of process parameters via the RSM method.

Porous gelatin-chitosan microcarriers (MCs) with the size of 350±50µm were fabricated with blends of different gelatin/chitosan (G/C) weight ratio using an electrospraying technique. Response surface methodology (RSM) was used to study the quantitative influence of process parameters, including blend ratio, voltage, and syringe pump flow rate, on MCs diameter and density. In the following, MCs of the same diameter and different G/C weight ratio (1, 2, and 3) were fabricated and their porosity and biocompatibility were investigated via SEM images and MTT assay, respectively. The results showed that mesenchymal stem cells (MSCs) could attach, proliferate, and spread on fabricated porous MCs during 7 days of culturing especially on those prepared with a G/C weight ratio of 1. Such porous gelatin-chitosan MCs with a G/C weight ratio of 1 may be considered as a promising candidate for injectable carriers supporting attachment and proliferation of MSCs.

Design, fabrication and characterization of oxidized alginate-gelatin hydrogels for muscle tissue engineering applications.

In this study, we reported the preparation of self cross-linked oxidized alginate-gelatin hydrogels for muscle tissue engineering. The effect of oxidation degree (OD) and oxidized alginate/gelatin (OA/GEL) weight ratio were examined and the results showed that in the constant OA/GEL weight ratio, both cross-linking density and Young's modulus enhanced by increasing OD due to increment of aldehyde groups. Furthermore, the degradation rate was increased with increasing OD probably due to decrement in alginate molecular weight during oxidation reaction facilitated degradation of alginate chains. MTT cytotoxicity assays performed on Wharton's Jelly-derived umbilical cord mesenchymal stem cells cultured on hydrogels with OD of 30% showed that the highest rate of cell proliferation belong to hydrogel with OA/GEL weight ratio of 30/70. Overall, it can be concluded from all obtained results that the prepared hydrogel with OA/GEL weight ratio and OD of 30/70 and 30%, respectively, could be proper candidate for use in muscle tissue engineering.

Fabrication and characterization of conductive chitosan/gelatin-based scaffolds for nerve tissue engineering.

This paper reports on the development of conductive porous scaffolds by incorporating conductive polyaniline/graphene (PAG) nanoparticles into a chitosan/gelatin matrix for its potential application in peripheral nerve regeneration. The effect of PAG content on the various properties of the scaffold is investigated and the results showed that the electrical conductivity and mechanical properties increased proportional to the increase in the PAG loading, while the porosity, swelling ratio and in vitro biodegradability decreased. In addition, the biocompatibility was evaluated by assessing the adhesion and proliferation of Schwann cells on the prepared scaffolds using SEM and MTT assay, respectively. In summary, this work supports the use of a porous conductive chitosan/gelatin/PAG scaffold with a low amount of PAG (2.5 wt.%) as a suitable material having proper conductivity, mechanical properties and biocompatibility that may be appropriate for different biomedical applications such as scaffold material in tissue engineering for neural repair or other biomedical devices that require electroactivity.