Microbiome modulation in inflammatory diseases: Progress to microbiome genetic engineering.

Recent developments in sequencing technology and analytical approaches have allowed researchers to show that the healthy gut microbiome is very varied and capable of performing a wide range of tasks. The importance of gut microbiota in controlling immunological, neurological, and endocrine function is becoming well-recognized. Thereby, numerous inflammatory diseases, including those that impact the gastrointestinal system, as well as less obvious ones, including Rheumatoid arthritis (RA), cancer, gestational diabetes (GD), type 1 diabetes (T1D), and type 2 diabetes (T2D), have been linked to dysbiotic gut microbiota. Microbiome engineering is a rapidly evolving frontier for solutions to improve human health. Microbiome engineering seeks to improve the function of an ecosystem by manipulating the composition of microbes. Thereby, generating potential therapies against metabolic, inflammatory, and immunological diseases will be possible through microbiome engineering. This essay first provides an overview of the traditional technological instruments that might be used for microbiome engineering, such as Fecal Microbiota Transplantation (FMT), prebiotics, and probiotics. Moreover, we will also discuss experimental genetic methods such as Metagenomic Alteration of Gut microbiome by In situ Conjugation (MAGIC), Bacteriophage, and Conjugative plasmids in manipulating intestinal microbiota.

Non-coding RNAs: A new frontier in benzene-mediated toxicity.

One of the most frequent environmental contaminants, benzene is still widely used as an industrial solvent around the world, especially in developing nations, posing a serious occupational risk. While the processes behind the toxicity of benzene grounds are not fully understood, it is generally accepted that its metabolism, which involves one or more reactive metabolites, is crucial to its toxicity. In order to evaluate the many ways that benzene could influence gene regulation and thus have an impact on human health, new methodologies have been created. The pathophysiology of the disorder may result from epigenetic reprogramming caused by exposure to benzene, including changes in non-coding RNA (ncRNA) markers, according to recent studies. We are interested in the identification of hazardous regulatory ncRNAs, the identification of these ncRNAs' targets, and the comprehension of the significance of these interactions in the mechanisms behind benzene toxicity. Hence, the focus of recent research is on long non-coding RNAs (lncRNAs), circular RNAs (circRNAs) and microRNAs (miRNAs), and some of the more pertinent articles are also discussed.

Bam Earthquake Survivors' Mental Health Status 12 Years after the Earthquake: A Population-Based Study.

BACKGROUND: Mental health is one of the characteristic benchmarks of public health. This research aimed to evaluate mental health of survivors of the Bam earthquake which occurred in 2003 twelve years after the earthquake in fall of 2015. METHODS: The statistical population of this cross-sectional study were people over 16 years of age who were present at the time of the earthquake in the city of Bam. The sample of the study consisted of 1500 people selected from the survivors by multistage sampling. The general health questionnaire-28 was used as a screening tool for mental disorders. The independent sample test, ANOVA test, Welch test, one-sample t test and the multiple logistic regression test were used to analyze the data. The analysis of data in the current study was carried out using the SPSS-18 software. RESULTS: Using the GHQ Likert scoring method, the results showed 52% of the people surveyed were suspected of having mental disorders (49.93% of men and 54.27% of women). Somatization and anxiety is more frequent than severe depression in respondents. The obtained statistics revealed that the prevalence of mental disorders amplified with loss of close relatives and age (odds ratio [OR] =1.457 and 1.828 respectively). The results also indicated that widows and divorcees (66.66%), illiterates and drop-outs (57.48%) and the elderly (77.12%) had the highest average in terms of having mental disorders. CONCLUSION: The results of this study showed more than half of the Bam earthquake survivors are suspected to be suffering from mental disorders. Since natural disaster-related mental illness effects can last for many years, comprehensive screening programs at regular intervals are required. Mental health services should be available to the elderly and people at risk in order to ensure restoration of mental health after a catastrophe.

Food Security and its Related Factors in the Rural Households of Bam City in Iran: A Logistic Regression Model Approach.

Awareness of the food security status of rural population and its influencing factors is essential for policy makers, public health institutions and the development of rural programs. This descriptive cross-sectional study was conducted in 2017 on 384 rural households in Bam city. The data was collected via the 6-item USDA questionnaire. Logistic regression was used to determine the relationship between social, economic and health factors with food security. The results indicate that 35.67% of households had full food security status, 24.47% had medium food security status and 7.04% had food insecurity status. Despite the high level of food insecurity in Bam rural households, creating employment opportunities for increasing household income, reforming inappropriate food habits, and increasing households' nutritional awareness might be effective in promoting food security.

Post-treatment Clinical Outcomes of Cutaneous Leishmaniosis in the Bam Area, South Eastern Iran: Analysis of over 9,000 Cases.

BACKGROUND: Knowledge about risk or protective factors for post-treatment outcomes in Cutaneous Lishmaniosis are rare, especially in endemic areas such as Iran. The present study aimed to evaluate the association between the outcome of infection, clinical manifestation, and treatment with adverse post-treatment outcomes in Cutaneous Lishmaniosis patients. METHODS: This was a cross sectional study based on recently collected data of 9077 Cutaneous Lishmaniosis patients (4585 female and 4492 male) from March 2003 to March 2011 in the Bam area, Iran. Multivariable multinomial logistic regression was applied to assess the effect of outcome of infection, clinical manifestation and treatment on relapse, treatment after interruption, treatment failure and clinical resistance. RESULTS: Head lesions were strongest risk factor for relapse (Odds Ratio, OR = 4.21; CI 95%: 3.56-4.98), treatment after interruption (2.00; 1.70-2.35), treatment failure (6.61; 5.17-8.45) and clinical resistance (2.62; 2.00-3.44). Family occurrence (yes vs. no), intra lesion therapy method, treatment duration (>3 v. ≤ 3 week) and source of detection by Surveillance (active vs. passive), were the most protective factors for relapse (OR = 0.58; CI 95%: 0.46-0.74), treatment after interruption (0.36; 0.31-0.42) treatment failure (0.24; 0.20-0.29) and clinical resistance (0.24; 0.09-0.67). CONCLUSION: Head lesions and treatment variables (e.g. therapy method and duration) could predict the occurrence of adverse post-term outcomes of Cutaneous Lishmaniosis. Further longitudinal studies have to clarify cause and effect relationships.