RESUMO
A bacterial strain PJ23T was isolated from the rhizosphere soil of Elymus dahuricus Turcz. sampled from a temperate semi-arid steppe in the northern of Inner Mongolia Autonomous Region, China. The strain is Gram-stain-negative, aerobic, light-pink, short rod-shaped, and non-spore-forming. Cell growth could be observed at 4-29â (optimal at 24â), pH 6.0-8.6 (optimal at 8.0) and in the presence of 0-5.0% (w/v) NaCl (optimal at 2.5%). The major cellular fatty acids of strain PJ23T were Summed feature 8 (C18:1 ω6c and/or C18:1 ω7c) (39.42%) and C16:0 (9.60%). The polar lipids were phosphatidylcholine, two unidentified glycolipids, one unidentified aminophospholipid, and two other unidentified polar lipids. The major respiratory quinone was ubiquinone-10. Phylogeny analysis based on 16S rRNA gene sequences retrieved from the genomes showed that, the strain was closely related to the species Terrihabitans soli IZ6T and Flaviflagellibacter deserti SYSU D60017T, with the sequence similarities of 96.79% and 96.15%, respectively. The G + C content was 65.23 mol% calculated on draft genome sequencing. Between the strains PJ23T and Terrihabitans soli IZ6T, the average nucleotide identity (ANI), amino acid identity (AAI) and digital DNA-DNA hybridization (dDDH) was 73.39%,71.12% and 15.7%, these values were lower than the proposed and generally accepted species boundaries of ANI, AAI and dDDH, respectively. Based on phenotypic, chemotaxonomic, and phylogenetic characteristics, strain PJ23T represents a novel species of Terrihabitans, for which the name Terrihabitans rhizophilus sp. nov. is proposed. The type strain is PJ23T (= KCTC 92977 T = CGMCC 1.61577 T).
Assuntos
Alphaproteobacteria , Rizosfera , Filogenia , RNA Ribossômico 16S/genética , Aminoácidos , Ácidos Graxos , DNARESUMO
A spherical, pink, aerobic, Gram-stain-positive bacterial strain (MIMF12T) was isolated from rhizosphere soil collected in the Inner Mongolia Autonomous Region, PR China. Cellular growth of the strain was observed at pH 6.0-8.0 (optimum, pH 7.0), at 20-37â°C (optimum, 28â°C) and with 0-1â% (w/v) NaCl (optimum, 0â%). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain MIMF12T was most closely related to Deinococcus terrestris SDU3-2T with a similarity value of 96.0â%. The respiratory quinone was menaquinone 8, the major fatty acids were C15â:â1 ω6c and C17â:â1 ω8c, and the major polar lipids were composed of two aminophospholipids, one phospholipid and four unidentified lipids. The G+C content of the genomic DNA was 70.1âmol%. The average nucleotide identity and digital DNA-DNA hybridization values between strain MIMF12T and the closest related type strain SDU3-2T were 88.1 and 52.1â%, respectively. The discovery that MIMF12T differs not only from validly named species in the genus Deinococcus, but also from currently unnamed species in the GDTB, gives us new insights into the genus. Based on phenotypic, chemotaxonomic and phylogenetic characteristics, strain MIMF12T represents a novel species of the genus Deinococcus, for which the name Deinococcus rhizophilus sp. nov. is proposed. The type strain is MIMF12T (=CGMCC 1.61579T=KCTC 43572T).