Towards more equitable cooling services of urban parks: Linking cooling effect, accessibility and attractiveness.

Global warming and rapid urbanization have caused frequent occurrences of heat waves and urban heat island effect, presenting a significant threat to health of urban residents. Researches have indicated that cooling services provided by parks are essential in alleviating impact of heat wave events and urban heat island effect. However, previous researches on park cooling services center around cooling effect, with a lack of exploration regarding the fairness of such services. To fill this gap, this study quantifies the level of equity in cooling services in 18 parks in the core area of Hangzhou. Through this study, we hope to clarify the current situation of fairness of cooling services in urban parks and provide fairer park cooling services through scientific and reasonable park layouts. This will alleviate the threat of rapid urbanization and climate change to urban residents, and make the urban environment develop in a more livable direction. We assessed the cooling effect using remote sensing and the ArcGIS platform to screen parks with cooling effect and to quantify their cooling service efficiency. We utilized spatial network analysis to quantify the accessibility and origin-destination matrix data to quantify the attractiveness to reflect the level of park cooling services. The results reveal that 18 parks exhibit a noticeable cooling effect, albeit with variations observed among parks. The percentage of urban parks with low accessibility is 77.80%, indicating that the distribution of accessible space presents an uneven status quo. In addition, 72.20% of parks have low attractiveness of cooling services, indicating that some parks have insufficient attractiveness of cooling services. Based on each indicator of cooling services, we categorize urban parks into four types based on supply and demand, and propose adaptive planning measures and intervention strategies to provide a reference for equitable distribution of cooling services in urban parks.

What Was George Forrest's Plant Collection Journey like in China?

Since the 16th century, Western countries have conducted extensive plant collections in Asia, particularly in China, driven by the need to collect botanical resources and foster academic development. These activities have not only significantly enriched the Western botanical specimen collections but have also had a profound impact on the development of related disciplines such as botany, ecology, and horticulture. During this process, a large number of renowned plant hunters emerged, whose discoveries and contributions are still remembered today. George Forrest (1873-1932) was one of these distinguished plant hunters. From 1904 to 1932, he visited China seven times to collect plants and became famous for the regional distinctiveness of the species he collected. However, due to the lack of systematic collection, organization, and analysis of specimens collected by Forrest, only a few species, such as the species Rhododendron, are well-known among the many species he introduced to the West. Furthermore, the personal collecting characteristics and the characteristic species collected by Forrest are also not clear. This limits a comprehensive understanding of the specimen collection history and impact of Forrest in China. Therefore, systematic organization and analysis of Forrest's plant specimens collected in China are crucial to understanding his impact on botanical classification, Rhododendrons introduction, global horticulture, and plant propagation. This study aims to systematically organize and analyze the plant specimens collected by George Forrest in China to investigate the family, genus, and species composition of the collected specimens and the seven collection expeditions of Forrest in China, as well as the time and altitude of these collections. Furthermore, it seeks to discuss Forrest's scientific contributions to the global spread of plants, the widespread application of the Rhododendron, and his impact on the development of modern gardens, providing a theoretical basis and data reference for related research and professional development. To this end, we extensively consulted important historical literature related to Forrest and systematically collected data from online specimen databases. The conclusions drawn from the available data include 38,603 specimens, with 26,079 collection numbers, belonging to 233 families, 1395 genera, and 5426 species, which account for 48.24%, 32.63%, and 14.17% of the plant families, genera, and species in China, respectively. Rhododendron specimens made up 17.20% of the specimens collected in this study. The collection locations cover three provinces or autonomous regions, 11 prefecture-level cities, and 25 counties. Furthermore, we found that Forrest's collections were concentrated in spring and summer, mainly in high-altitude areas, with 135 species found below 1500 m and 3754 species at 1500 m and above. Rhododendron specimens were mostly found above 3000 m.

Shall we promote natural history collection today?-Answered by reviewing Ernest Henry Wilson's plant collection process in China.

Plant diversity exploration needs to be accelerated because many species will go extinct before their discovery and description, and many species-rich regions remain poorly studied. However, most contemporary plant collections prefer to focus on a specific group, which hinders the exploration and conservation of plant diversity. Therefore, we need an alternative approach to the dilemma at hand. The comprehensive Natural History Collection (NHC), which existed throughout the pinnacle of biodiversity exploration in the 20th century could be considered. We explore Ernest Henry Wilson's (one of the most successful naturalists in the 20th) plant collections in China as a case to illustrate the advantages of NHC and discuss whether NHC deserves to be promoted again today. From multiple sources, we gathered 19,218 available specimen records of 11,884 collecting numbers assigned and analyzed the collected species, the collection's nature, and restored four routes of his explorations. Results reveal that Wilson's specimens were collected from 28 prefecture-level cities and 38 county-level regions of 7 provinces or municipalities, they belong to 200 families, 1046 genera, 3794 species, and 342 infraspecific taxa, approximately 41 %, 22 %, 10 %, 5 % of Chinese plant families, genera, species, and infraspecific taxa respectively. The Wilson case study shows that NHC is particularly effective in emphasizing species discovery and conservation, recording ecological information, understanding a region's flora, and developing landscape applications. Therefore, we strongly advocate for the expansion of natural history collections in species-rich regions. Furthermore, we recommend the employment of specialized collectors, the enlistment of international cooperation, and the standardization of guidelines for future NHCs.

Alternative scenarios for urban tree surveys: Investigating the species, structures, and diversities of street trees using street view imagery.

Grasping information about street trees can assist urban environmental managers in quantifying and evaluating their costs and ecological benefits. Street view imagery has the potential for urban street tree surveys. However, few studies have been conducted on the inventory of street tree species, size structures and diversity based on street view imagery at the urban scale. In this study, we tried to conduct a survey of street trees in urban areas of Hangzhou using street view images. First, we constructed a size reference items system and determined that using it for street view measurements of street trees was comparable to field measurements results (R2 = 0.913-0.987). On this basis, we investigated the distribution characteristics and differences of street trees in Hangzhou using Baidu Street View and found that Cinnamomum camphora was the dominant tree species in Hangzhou (46.58 %), and the high proportion made urban street trees susceptible to ecological hazards. In addition, surveys conducted separately in various urban districts revealed that the diversity of street trees in new urban areas was smaller and less uniform. Additionally, as the gradient got further away from the city center, the street trees are smaller, the diversity first increased and then decreased, and the evenness gradually decreased. This study analyzes the use of Street View to investigate the distribution of species, size structure, and diversity of urban street trees. The use of street view imagery will simplify the collection of data on urban street trees and provide urban environmental managers with a foundation for strategy development.

Simulating future land use by coupling ecological security patterns and multiple scenarios.

A land use simulation model with coupling constraints of ecological security patterns (ESPs) and multiple scenarios (MSs) was developed using the PLUS model. The research scale was zoned with environmental functional regions, where land management policies were formulated. A case study in Anji County successfully demonstrated the application of the ESP-MS-PLUS model. First, we constructed three different levels of ESPs as ecological constraints by utilizing ecosystem services evaluation and circuit theory. Second, four scenarios of land use and land cover changes (LUCCs) in 2034 were assumed, namely business as usual (BAU), priority given to urban development (PUD), priority given to ecological protection (PEP), and balanced urban development and ecological protection (BUE). Then, the basic ecological constraints (ecological red line areas and waters) and three types of ESPs were coupled with the four scenarios. The results of the simulation and analysis of landscape metrics under each scenario showed that the PEP and BUE scenarios would effectively reduce the degree and speed of ecological destruction. In addition, there were three environmental functional areas that could be used as priority areas for urban construction to ensure economic development. This study provides a new mechanism for land use optimization in the context of ecological protection at scales conducive to practice.

Influence of view factors on intra-urban air temperature and thermal comfort variability in a temperate city.

Urban geometry is known to be one of the major factors in explaining the intra-urban temperature variations. A commonly used indicator to describe the urban geometry is the sky view factor. However, the existing studies have shown that the relationship between SVF and urban temperature is quite contradictory. This suggests that a single SVF cannot accurately quantify the urban geometry. For comparison, we here propose to use view factors, including sky, building and tree view factors (SVF, BVF, and TVF, respectively), to accurately quantify the three-dimensional urban geometry. Based on microclimate measurements conducted in Beijing Olympic Park and its surrounding urban environment in Beijing, China, the impact of view factors on intra-urban air temperature and thermal comfort was evaluated. Measurements were conducted along a selected path during hot summer days with clear skies and light winds by mobile traverses. The obtained results showed that SVF was positively correlated with air temperature during the day but negatively correlated with air temperature at night. BVF mainly played a warming role in both daytime and nighttime. Especially at night, BVF was the main geometric warming factor. TVF had a significant cooling effect during the day but did not have a negative effect at night due to reduced SVF. There was a strong point-to-point correlation between SVF and outdoor thermal comfort in the daytime. The mean differences in Mean Radiant Temperature and Physiologically Equivalent Temperature between shaded and unshaded sites were 12.0 °C and 6.8 °C, respectively, which suggested that providing effective shading is extremely important for improving outdoor daytime thermal comfort.

A water-soluble molecular probe with aggregation-induced emission for discriminative detection of Al3+ and Pb2+ and imaging in seedling root of Arabidopsis.

Luminogens with aggregation-induced emission (AIE) have been used to develop a new type of molecular probes based on analyte-triggered aggregation, but it still remains a challenge to design water-soluble AIE-active probe for specific detection of metal ions. Herein, we designed and synthesized a water-soluble molecular probe with AIE property for discriminative detection of aluminum ion and lead ion. Four carboxylic acid groups were incorporated into a tetraphenylethylene unit to enhance the coordination affinity and increase water-solubility in aqueous solution. The designed probe can be selectively lighted up by aluminum ion and lead ion via coordination-triggered AIE process. Discrimination of aluminum ion and lead ions based on the probe can be achieved in quantitative manner with the assistance of suitable masking reagents. This probe was further used to image aluminum ions in living cells of seedling roots of Arabidopsis, and the results showed that this probe is capable of imaging aluminum ions in living cells avoiding the interference of lead ions, and is suited for long-term imaging due to its excellent photostability. This work expands the application scope of AIE-active probes in discriminative detection of metal ions, and provides a design direction for water-soluble AIE probes to avoid the false signals from self-precipitation under physiological conditions.

Study on different particulate matter retention capacities of the leaf surfaces of eight common garden plants in Hangzhou, China.

The severity of inhalable particulate matter (PM) pollution in the atmosphere is increasing; however, plants can effectively reduce the concentration of atmospheric PM by retaining it on their leaves. In this paper, eight common garden plants in Hangzhou, China, were selected as the study objects to observe the morphological features of the leaf surfaces and the retained particles and to analyze the elemental composition of the particles. Confocal laser scanning microscopy (CLSM) was performed to detect the morphological features of the leaf surfaces, and the relationship between the roughness of the leaf surface and the number of the retained particulates was quantitatively analyzed. In addition, the elements in the soil were measured via inductively coupled plasma-optical emission spectrometry (ICP-OES) to locate the possible particulate sources. The results revealed that leaves are able to retain particulates via the synergy of multiple microstructures on the leaf surface, such as grooves, folds, small chambers, flocculus projections, long villi, pubescent hairs and waxes. Moreover, the leaf surface roughness is closely related to the number of retained particulates, with rougher surfaces corresponding to more rugged folds and grooves and a stronger retention ability. The retained particulates are primarily composed of C, O, Si, Al, Ca, K, Mg, Nb, Fe, Na and Ti, and a comparison with the elements in the soil samples indicated that these elements originated from soil dust. Among the different particle sizes, PM with a diameter <2.5 µm (PM2.5) presented the greatest retention on the surfaces of the different plant leaves, while a much smaller amount of PM with a diameter larger than 10 µm was retained. The research results provide an important theoretical scientific basis for the mechanism underlying PM adsorption by plants and strategies for the reasonable selection of garden dust-retaining tree species.

The Emission of the Floral Scent of Four Osmanthus fragrans Cultivars in Response to Different Temperatures.

Floral scent is an important part of volatile organic compounds (VOCs) emitted from plants, and is influenced by many environmental and endogenous factors. To investigate the influence of temperature on the emission of the floral scent of Osmanthus fragrans, the number of chemical compounds and their relative release amounts from four cultivars of O. fragrans under different temperature treatments, were identified using the solid-phase microextraction (SPME) technique and gas chromatography-mass spectrometry (GC-MS) in this study. Results revealed that the numbers and release amounts of floral scent components were significantly influenced by different temperatures, and depend on different cultivars and different types of compounds. Overall, most cultivars had the largest number of chemical compounds in 19 °C and the numbers of chemical compounds decreased with the increase or decrease in the temperature. Alcohols and ketones were the two main kinds of compounds responding to temperature change. The response of a specific chemical compound to temperature change was different in four cultivars. Generally, linalool, α-ionone, ß-ionone, and γ-decalactone accounted for the highest proportion in the nine main compounds, and changes of these four chemical compounds to different temperatures had obvious contributions to the floral scent of O. fragrans. The results obtained provide evidence that temperatures can greatly influence the emission of floral scent.

Identification of Suitable Reference Genes for Gene Expression Normalization in the Quantitative Real-Time PCR Analysis of Sweet Osmanthus (Osmanthus fragrans Lour.).

Quantitative real-time PCR (RT-qPCR), a sensitive technique for quantifying gene expression, depends on the stability of the reference gene(s) used for data normalization. Several studies examining the selection of reference genes have been performed in ornamental plants but none in sweet osmanthus (Osmanthus fragrans Lour.). Based on transcriptomic sequencing data from O. fragrans buds at four developmental stages, six reference genes (OfACT, OfEF1α, OfIDH, OfRAN1, OfTUB, and OfUBC2) with stable expression (0.5 to 2 fold change in expression levels between any two developmental stages), as well as the commonly used reference gene Of18S, were selected as candidates for gene expression normalization in the RT-qPCR analysis of O. fragrans. For the normalization of RT-qPCR with two dyes, SYBR Green and EvaGreen, the expressional stability of seven candidate reference genes in 43 O. fragrans samples was analyzed using geNorm, NormFinder and BestKeeper. For RT-qPCR using SYBR Green, OfRAN1 and OfUBC2 were the optimal reference genes for all samples and different cultivars, OfACT and OfEF1α were suitable for different floral developmental stages, and OfACT was the optimal reference gene for different temperature treatments. The geometric mean values of the optimal reference gene pairs for the normalization of RT-qPCR are recommended to be used for all samples, different cultivars and different floral developmental stages in O. fragrans. For RT-qPCR using EvaGreen, OfUBC2 was the optimal reference gene for all samples and different cultivars, and OfACT was the optimal reference gene for different floral developmental stages and different temperature treatments. As the worst reference gene, Of18S should not be used as a reference gene in O. fragrans in the future. Our results provide a reference gene application guideline for O. fragrans gene expression characterization using RT-qPCR.

Effects of osmotic stress on antioxidant enzymes activities in leaf discs of PSAG12-IPT modified Gerbera.

Leaf senescence is often caused by water deficit and the chimeric gene P(SAG12)-IPT is an auto-regulated gene delaying leaf senescence. Using in vitro leaf discs culture system, the changes of contents of chlorophylls, carotenoids, soluble protein and thiobarbituric acid reactive substance (TBARS) and antioxidant enzymes activities were investigated during leaf senescence of P(SAGl2)-IPT modified gerbera induced by osmotic stress compared with the control plant (wild type). Leaf discs were incubated in 20%, 40% (w/v) polyethylene glycol (PEG) 6000 nutrient solution for 20 h under continuous light [130 micromol/(m(2) x s)]. The results showed that the contents of chlorophylls, carotenoids and soluble protein were decreased by osmotic stress with the decrease being more pronounced at 40% PEG, but that, at the same PEG concentration the decrease in the transgenic plants was significantly lower than that in the control plant. The activities of superoxide dismutase (SOD), catalases (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (GPX) and dehydroascorbate reductase (DHAR) were stimulated by PEG treatment. However, the increases were higher in P(SAG12)-IPT transgenic plants than in the control plants, particularly at 40% PEG treatment. Lipid peroxidation (TBARS content) was increased by PEG treatment with the increase being much lower in transgenic plant than in the control plant. It could be concluded that the increases in the activities of antioxidant enzymes including SOD, CAT, APX, GPX and DHAR were responsible for the delay of leaf senescence induced by osmotic stress.

Assessing the willingness of the public to pay to conserve urban green space: the Hangzhou City, China, case.

The authors assessed the willingness of residents to pay for urban green-space conservation in Hangzhou, China, using the contingent-valuation method. The aim of the study was to provide policy makers with information that would be useful for making informed decisions in urban-development planning. The findings of the study are as follows: 1) The willingness of residents to pay for urban green-space conservation was positively correlated with their perceptions of the benefits of green spaces and negatively correlated with perceptions of the annoyances. 2) The willingness to pay a higher premium for green-space conservation is directly related to gender, income level, and residential-ownership status. Age and education level are not significantly correlated with willingness to pay. 3) A majority of respondents view the conservation of urban green spaces as a very important function of the city, and most of them are willing to pay additional taxes for this conservation. 4) The total value per year to the public of the conservation program in Hangzhou is about $15.4 million. These qualitative and quantitative findings can be used in the policy-making process for urban-development plans.

Rapid growth of polymer brushes from immobilized initiators.

This report describes the remarkably rapid synthesis of polymer brushes under mild conditions (50 degrees C) using surface-initiated polymerization. The highly active atom transfer radical polymerization catalyst Cu(I)-1,4,8,11-tetramethyl-1,4,8,11-tetraazacyclotetradecane allows synthesis of 100 nm thick poly(tert-butyl acrylate) brushes from initiator-modified Au surfaces in just 5 min. Using the same catalyst, polymerization of 2-hydroxyethyl methacrylate and methyl methacrylate yielded 100 nm thick films in 10 and 60 min, respectively. Such growth rates are an order of magnitude greater than those for traditional free-radical polymerizations initiated from surfaces. These polymerizations also retain some features of controlled radical polymerizations, such as the ability to form block copolymer brushes.

High-capacity binding of proteins by poly(acrylic acid) brushes and their derivatives.

Polymeric coatings with high protein-binding capacities are important for increasing the output of affinity-based protein purification and decreasing the detection limits of antibody microarrays. This report describes the use of thick poly(acrylic acid) (PAA) brushes to immobilize as much as 80 monolayers of protein. The brushes were prepared using a recently developed procedure that allows polymerization of 100-nm-thick poly(tert-butyl acrylate) films from a surface in just 5 min along with hydrolysis of these films to PAA in 15 min. Covalent binding of bovine serum albumin (BSA) to PAA brushes that were activated using standard coupling agents, however, resulted in immobilization of less than two monolayers of BSA because of competitive hydrolysis of the esters in the activated film. In contrast, derivatization of PAA with nitrilotriacetate (NTA)-Cu2+ complexes yielded films capable of binding many monolayers of protein via metal-ion affinity interactions. For example, derivatization of 55-nm-thick PAA films with NTA-Cu2+ allowed immobilization of about 15 monolayers (5.8 microg/cm2 or 58 nm) of BSA. The binding capacity was even higher for myoglobin (7.7 microg/cm2) and anti-IgG (9.6 microg/cm2). Remarkably, electrostatic adsorption of lysozyme in 55-nm-thick, underivatized PAA resulted in as much as 80 monolayers (16.2 microg/cm2 or 162 nm) of adsorbed protein. Polymer synthesis, derivatization, and swelling, as well as BSA immobilization kinetics and thermodynamics were characterized using reflectance FT-IR spectroscopy, ellipsometry, and protein assays.

Silicon-mediated alleviation of Mn toxicity in Cucumis sativus in relation to activities of superoxide dismutase and ascorbate peroxidase.

The effects of exogenous silicon (Si) on plant growth, activities of superoxide dismutase (SOD), guaiacol peroxidase (GPX), ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), glutathione reductase (GR) and catalase, and concentrations of ascorbate and glutathione were investigated in cucumber (Cucumis sativus L.) plants treated with excess manganese (Mn) (600 microM). Compared with the treatment of normal Mn (10 microM), excess Mn significantly increased H2O2 concentration and lipid peroxidation indicated by accumulation of thiobarbituric acid reactive substances. The leaves showed apparent symptoms of Mn toxicity and the plant growth was significantly inhibited by excess Mn. The addition of Si significantly decreased lipid peroxidation caused by excess Mn, inhibited the appearance of Mn toxicity symptoms, and improved plant growth. This alleviation of Mn toxicity by Si was related to a significant increase in the activities of SOD, APX, DHAR and GR and the concentrations of ascorbate and glutathione.