Morphological analysis of colon goblet cells and submucosa in type I diabetic rats submitted to physical training.

Colon layers, especially the submucosa, as well as the secretion of goblet cells are extremely important for the functioning and transit of substances in this organ. However, the damages arising from type I diabetes and the effects of physical training, which plays crucial role in the treatment of this disease, are not yet known in these regions. To analyze the changes in colon submucosa and goblet cells of diabetic rats, as well as the effects of physical training, Wistar rats were divided into four groups: sedentary control, trained control, sedentary diabetic (SD), and trained diabetic (TD). The training protocol consisted of swimming for 60 min a day, 5 days per week, during 8 weeks. Colon samples were collected, processed, and evaluated by histochemical and ultrastructural techniques. Goblet and submucosa cells did not show alterations in shape, size, protein and carbohydrate content, in all treatment groups. Decreased amount of collagen fibers, however, was observed in the submucosa and lamina propria of SD rats, but this alteration was recovered in TDs. The ultrastructural analysis, in turn, revealed greater quantity of Golgi apparatus cisterns in SDs, distinctly than TDs, which showed improvement in this diabetic condition. Thus, physical training was responsible for the recovery of some important diabetic alterations, possibly improving the motility of substances in the large intestine. Nevertheless, it cannot be considered alone in the treatment of this disease, requiring the combined practice of other methods.

Efeitos em curto prazo da aplicação de aloxana para indução de diabetes em ratos Wistar / Effects in short-term of alloxan application to diabetes induction in Wistar rats

O presente estudo teve como objetivo verificar a ação da aloxana em parâmetros metabólicos e do sistema imune 24 e 192 horas após sua aplicação em ratos Wistar. Para isso, oito ratos foram mantidos em jejum de 12 horas e receberam aloxana monoidratada, Sigma (32 mg/kg de peso corporal) via endovenosa. Foram registrados os valores de massa corporal, ingestão hídrica e alimentar e realizada a contagem de leucócitos totais previamente, 24 e 192 horas após a aplicação da aloxana. As análises de glicemia e trigliceridemia foram realizadas previamente e 192 horas após a aplicação da aloxana. Após 24 horas, a aplicação da aloxana causou aumento da ingestão hídrica e redução da massa corporal, ingestão alimentar e contagem de leucócitos. Na avaliação realizada 192 horas após a aplicação da aloxana houve recuperação da ingestão alimentar e contagem de leucócitos. Por outro lado, neste período houve aumento da glicemia e ingestão hídrica além de redução da massa corporal. Isso indica que parte dos sinais de diabetes causados pela aloxanamanifesta-se em curto prazo da administração da droga.

The present study aimed to verify action of alloxan in metabolic and immune parameters after 24 and 192 hours of the injection in Wistar rats. Thus, eight rats were fasted and received monohidrated alloxan Sigma (32 mg/kg body weight) via endovenous. Glycemia and trglyceridemia analyzes were performed before and 192 hours after alloxan application. After 24 hours, alloxan application increased water intake and decreased body mass, food intake and leucocytes counting. 192 hours after alloxan application, there was a recuperation in food intake and leucocytes counting. On the other hand, in this period there was an increase of glycemia and water intake and reduction of body mass. These results indicate that some of diabetic signs caused by alloxan occur in short-term after drug administration.