RESUMO
Neospora caninum is an obligatory intracellular protozoan parasite, phylum Apicomplexa. Canids are definitive hosts and different animals can be intermediate hosts. Neospora DNA has been also detected in humans, recently. This study aimed to understand the infection rate of N. caninum in chicken meat because consumption of raw and undercooked meat can be the main risk factor for canine neosporosis. Investigation of Neospora vertical transmission to the eggs is also important. One hundred chicken legs, and fifty eggs from free-range chickens, and fifty eggs from industrial chickens were collected from different stores in Semnan city, Iran. Deoxyribonucleic acid (DNA) of samples was extracted, and the Nested-PCR (polymerase chain reaction) on Neospora internal transcribed spacer (ITS-1) gene was performed. Neospora caninum DNA was detected in eight out of one hundred (8%) chicken legs, and no eggs were infected. These results revealed that N. caninum infection in chicken meat for the first time in Iran. For the investigation of Neospora vertical transmission to eggs, more studies will be necessary. Indoor carnivores should be fed, and humans should be consumed well-cooked chicken meat to prevent infection.