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1.
Insect Biochem Mol Biol ; 32(11): 1525-32, 2002 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-12530220

RESUMO

The male Jeffrey pine beetle, Dendroctonus jeffreyi Hopkins (Coleoptera: Scolytidae), produces the bicyclic ketal frontalin as part of a complex semiochemical blend. A key regulated enzyme in the mevalonate pathway, 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-R), showed high transcript levels in the anterior midgut of male Jeffrey pine beetles by in situ hybridization. HMG-R expression in this area of the alimentary canal was related to male emergence, where emerged males demonstrated significant up-regulation of HMG-R transcript and pre-emerged males showed only basal levels. Pre-emerged males were induced to express high levels of HMG-R transcript by treatment with juvenile hormone (JH) III. Additionally, isolated anterior midgut tissue from JH III-treated males converted radiolabeled acetate to frontalin, as assayed by radio-HPLC, providing strong evidence that this is the site of frontalin production in male beetles.


Assuntos
Compostos Bicíclicos Heterocíclicos com Pontes/metabolismo , Besouros/fisiologia , Fenômenos Fisiológicos do Sistema Digestório , Feromônios/biossíntese , Pinus/parasitologia , Acetatos/metabolismo , Animais , Besouros/anatomia & histologia , Besouros/genética , Especificidade de Órgãos
2.
Insect Biochem Mol Biol ; 30(12): 1203-11, 2000 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-11044666

RESUMO

We have isolated a full length 3-hydroxy-3-methylglutaryl coenzyme A synthase (HMG-S) cDNA from the male Jeffrey pine beetle, Dendroctonus jeffreyi Hopkins, and studied the effects of topical applications of juvenile hormone III (JH III) on its expression. The predicted translation product of this apparently single copy gene has 63% and 58% identity with HMG-S1 and HMG-S2 from Blattella germanica (L.), and 61% identity with Drosophila melanogaster Hmgs. HMG-S transcript levels remain uniformly low in JH III-treated and control D. jeffreyi females, but are induced approximately 2.5- to 5-fold in JH III-treated males. JH III causes a dose- and time-dependent increase in HMG-S transcripts in the male metathoracic-abdominal region. Since monoterpenoid pheromone precursor synthesis and HMG-CoA reductase expression are under the control of JH III in the metathorax of Ips bark beetles, the observed HMG-S expression pattern suggests that the isoprenoid pathway is similarly important for semiochemical production in D. jeffreyi.


Assuntos
Besouros/enzimologia , Regulação Enzimológica da Expressão Gênica , Hidroximetilglutaril-CoA Sintase/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Besouros/genética , DNA Complementar , Sistema Endócrino/metabolismo , Dosagem de Genes , Humanos , Hidroximetilglutaril-CoA Sintase/isolamento & purificação , Hidroximetilglutaril-CoA Sintase/metabolismo , Masculino , Dados de Sequência Molecular , Homologia de Sequência de Aminoácidos , Distribuição Tecidual , Árvores
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