Mangaba (hancornia speciosa): exploring potent antifungal and antioxidant properties in lyophilised fruit pulp extract through in vitro analysis.

Mangaba is a fruit native to Brazil, rich in bioactive compounds. To evaluate physicochemical composition, bioactive compounds, antioxidant and antifungal activity of mangaba fruit pulp. Moisture, ash, protein, lipid, energy values and phenolic compounds were determined. Antioxidant activity was determined by capture of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. Evaluation of antifungal activity was performed by Minimum Inhibitory Concentration, according to protocols M07-A9 and M27-S3, and minimum fungicidal concentration. Freeze-dried mangaba pulp presented high levels of carbohydrates, low levels of lipids, and high energy density. Phenolic analysis demonstrated that chlorogenic acid was found in the highest concentration, followed by p-coumaric acid and ferulic acid. Mangaba extract showed antioxidant activity like BHT. Mangaba extract inhibited the growth of Candida albicans (ATCC 90028), Cryptococcus gattii (AFLP4), Candida guilliermondii (ATCC 6260) and Candida albicans (MYA 2876). Freeze-dried mangaba inhibited fungal activity associated with antioxidant effect due to presence of phenolic compounds.

Liver transplant in patients with primary sclerosing cholangitis: A retrospective cohort from Northeastern Brazil.

BACKGROUND: Primary sclerosing cholangitis (PSC) manifests within a broad ethnic and racial spectrum, reflecting different levels of access to health care. AIM: To evaluate the clinical profile, complications and survival rates of patients with PSC undergoing liver transplantation (LTx) at a Brazilian reference center. METHODS: All patients diagnosed with PSC before or after LTx were included. The medical records were reviewed for demographic and clinical variables, including outcomes and survival. The level of statistical significance was set at P < 0.05. RESULTS: Our cohort represented 1.6% (n = 34) of the 2113 patients receiving liver grafts at our service over the past two decades. Most were male (n = 19; 56%). The average age (40 ± 14 years) was similar for men and women (P = 0.347). The mean follow-up time from diagnosis to LTx was 68 mo. Most patients had the classic form of PSC. Three women had PSC/autoimmune hepatitis overlap syndrome, and one patient had small-duct PSC. Alkaline phosphatase levels at diagnosis and pre-LTx model for end-stage liver disease. scores were significantly higher in males. Inflammatory bowel research (IBD) was investigated by colonoscopy in 26/34 (76%) and was present in most cases (18/26; 69%). IBD was less common in women than in men (44.4% vs. 55.6%) (P = 0.692). Cholangiocarcinoma (CCA) was diagnosed in 2/34 (5.9%) patients by histopathology of the explant (survival: 3 years 6 mo, and 4 years 11 mo). Two patients had complications requiring a second LTx (one after 7 d due to hepatic artery thrombosis and one after 17 d due to primary graft dysfunction). Five patients (14.7%) developed biliary stricture. The overall median post-LTx survival was 66 mo. Most deaths occurred in the first year (infection n = 2, primary liver graft dysfunction n = 3, unknown cause n = 1). The 1-year and 5-year survival rates of this cohort were 82.3% and 70.6%, respectively, matching the mean overall survival rates of LTx patients at our center (87.1% and 69.43%, respectively) (P = 0.83). CONCLUSION: Survival after 1 and 5 years was similar to that of other LTx indications. The observed CCA survival rate suggests CCA may be an indication for LTx in selected cases.

A prática do kitesurf e as lesões relacionadas: uma revisão narrativa da literatura / Kitesurfing practice and related injuries: a narrative review of the literature

Objetivo: realizar uma revisão na literatura para caracterizar o perfil epidemiológico das lesões traumáticas provocadas pela prática dessa modalidade. Metodologia: realizou-se uma revisão narrativa da literatura acerca das lesões decorrentes da prática esportiva do kitesurf na base de dados da Scielo, Ovid MEDLINE e Google Scholar de artigos de janeiro de 2000 a dezembro de 2022. Resultados: foram eleitos 14 artigos que se ajustaram aos objetivos dessa revisão. Mediante análise dos artigos, foram encontradas discussões que direcionam os principais mecanismos de trauma, riscos de lesões, estruturas mais frequentemente acometidas e propostas para minimizar os danos e a recuperação. Conclusão: as lesões que podem acometer os praticantes deste esporte aquático, em sua maioria, são leves e principalmente nos membros inferiores; entretanto, podem ser letais, o que desperta a necessidade de maior investigação epidemiológica e clínica dos casos atendidos, bem como a adoção de medidas de prevenção.

Objective: the objective of this study is to conduct a literature review to characterize the epidemiological profile of traumatic injuries caused by the practice of this modality. Methods: a narrative review of the literature was carried out on injuries resulting from the practice of kitesurfing in the database of Scielo, Ovid MEDLINE, and Google Scholar articles from January 2000 to December 2022. Results: 14 articles were chosen that suited the objectives of this review. Through the analysis of the articles, discussions were found that guide the main mechanisms of trauma, risk of injuries, structures most frequently affected, and proposals to minimize damage and recovery. Conclusion: the injuries that can affect practitioners of this aquatic sport are mostly mild and mainly in the lower members; however, they can be lethal, which raises the need for further epidemiological and clinical investigation of the cases treated, as well as the adoption of measures of prevention.

Marcadores de alimentação e excesso de peso nas macrorregiões de saúde de Mato Grosso do Sul: 2015 -2022 / Marqueurs d'alimentation et de surpoids dans les macrorégions de santé du Mato Grosso do Sul : 2015 -2022

Em uma primeira análise de tendências nos estado de Mato Grosso do Sul é possível observar em relação aos marcadores do SISVAN para obesidade/excesso de peso em suas formas mais graves segundo a faixa etária, que existem tendências ao aumento em todas as situações apresentadas com exceção dos menores de 5 anos, que apresentam indícios de queda. Aliado às tendências de aumento da obesidade/excesso de peso em suas formas mais grave nas diferentes faixas etárias no estado, podemos ver nos marcadores de consumo que existe uma tendência na estabilização do consumo de feijão, que marcaria alimentação in natura e tradicional do brasileiro, e uma tendência de aumento do consumo de alimentos ultraprocessados, que são amplamente associados à obesidade e doenças crônicas na literatura (LOUZADA et al, 2021). Os dois fatores anteriormente descritos, associados aos dados de alta prevalência de inatividade física, evidentes nos dados do COVITEL (2022) de que apenas 1/3 da população adulta/idosa do Centro-Oeste é fisicamente ativa (com tendência de queda entre 2021 e 2022) vem nos trazer uma situação alarmante em relação aos principais fatores de controle para cuidado da obesidade em nosso estado (Vital Strategies Brasil, 2023). A Linha de Cuidado de Sobrepeso e Obesidade deve responder à esses desafios com pactuações coletivas para melhor resolubilidade do SUS. Consumo de Feijão faz parte do grupo "Alimentos in natura", poisnão há adição de outros aditivos ou nutrientes para seu consumo. Além disso, o feijão é marco cultural da alimentação brasileira denorte a sul no país. No esquema abaixo, ele entra na barra verde da recomendação, que deve ser consumido em maior quantidade. O consumo de alimentos ultraprocessados é um marcador que engloba todos os alimentos ultraprocessados, que tiveram consumo referido no dia anterior pelo usuário. O consumo desses alimentos está relacionado com Obesidade e DCNT. No esquema abaixo, essa classe de alimentos entra na barra marrom da recomendação, e deve ser evitado ou consumido deve ser reduzido ao mínimo possível. A seguir será descrita a evolução temporal dos marcadores de consumo feijão e ultraprocessados e do IMC para faixa etária coletados na APS nas interfaces de ações da Vigilância Alimentar e Nutricional (VAN). Todos os dados foram obtidos pelo SISVAN-Web para o território do estado do Mato Grosso do Sul segundo macrorregiões de saúde, por meio de relatórios públicos oficiais. Os relatórios públicos para marcadores de consumo e de antropometria podem ser acessados oficialmente pelo link: https://sisaps.saude.gov.br/sisvan/relatoriopublico/index. Esses relatórios foram coletados no período de maio a julho de 2023, referentes aos anos de 2015 a 2022, segundo pactuado em GT da LCSO para apresentação da situação em Comitê Intergestores Regional (CIR), Comitê Intergestores Bipartite (CIB) e para o Conselhos de Secretarias Municipais de Saúde de Mato Grosso do Sul (COSEMS - MS). As análises de tendência temporal foram realizadas por meio de regressão de Linear, com auxílio do programa Power BI (versão gratuita), dentro da sua função DAX (Data Analysis Expressions). Em suma, as prevalências crescentes de doenças crônicas e obesidade no Brasil refletem mudanças nos padrões de vida e nos hábitos alimentares, bem como desafios socioeconômicos. Lidar com esses problemas requer uma ação coordenada de governos, profissionais de saúde, indústria alimentícia e a sociedade em geral, com foco na promoção da saúde, prevenção e acesso equitativo a serviços de saúde de qualidade.

Responsabilidade, consentimento e cuidado. Ética e moral nos limites da sexualidade / Responsibility, consent and care. Ethics and morals at the limits of sexuality / Responsabilidad, consentimiento y cuidado. Ética y moral en los límites de la sexualidad

Resumo Neste artigo pretendo apresentar alguns dos resultados de uma pesquisa de pós- -doutoramento, na qual acompanhei grupos virtuais e encontros presenciais de interlocutores adeptos de práticas sexuais tidas como "de risco", tais como o sexo bareback, em eventos exclusivos para homens cisgêneros. O objetivo aqui é analisar um certo regime de regulação ético-moral percebido em campo relativo à preocupação de se afirmar que essas práticas sexuais partem de valores como a "responsabilidade", o "consentimento" e o "cuidado". Nas dinâmicas observadas, esses valores se (re)constroem e fissuram a partir do atravessamento de elementos diversos como drogas e medicamentos, além de diversos tensores libidinais. É sobre a produção contextual desses conceitos e a sua relação frente às atuais políticas pública de prevenção que pretendo me debruçar no artigo.

Abstract This article presents some results of a postdoctoral research, in which I followed virtual groups and face-to-face meetings of interlocutors engaging at sexual practices considered "risky", such as bareback sex, in events exclusive for cisgender men. The objective here is to analyze a certain regime of ethical-moral regulation perceived in the fieldwork regarding a concern to assign to these sexual practices values such as "responsibility", "consent" and "care". In the observed dynamics, these values are (re)constructed and fissured at the intersection of diverse elements such as drugs and medications, in addition to several libidinal tensors. This article addresses these concepts contextual production and their relation to current public prevention policies.

Resumen En este artículo tengo la intención de presentar algunos de los resultados de una investigación posdoctoral, en la que seguí grupos virtuales y reuniones cara a cara de interlocutores que eran expertos en prácticas sexuales consideradas "en riesgo", como el sexo a pelo, en eventos exclusivos para hombres cisgénero. El objetivo aquí es analizar un cierto régimen de regulación ético-moral percibido en el campo con respecto a la preocupación de afirmar que estas prácticas sexuales parten de valores tales como "responsabilidad", "consentimiento" y "cuidado". En la dinámica observada, estos valores se (re) construyen y descifran a través del cruce de diferentes elementos, como drogas y medicamentos, además de varios tensores libidinales. Se trata de la producción contextual de estos conceptos y su relación con las políticas actuales de prevención pública que pretendo abordar en el artículo.

Dual Electrolytic Eluent Generation for Oligosaccharides Analysis Using High-Performance Anion-Exchange Chromatography.

The research on oligosaccharides is growing and gaining in importance at a rapid pace. The efforts to understand their bioactivity and to develop new products based on oligosaccharides in biotherapeutics and food industry require effective and reliable tools for analysis of oligosaccharides. Here we present a dual electrolytic eluent generation platform for the analysis of oligosaccharides by high-performance anion-exchange liquid chromatography (HPAE) in both analytical and capillary column formats. The system consists of one eluent generator producing methanesulfonic acid (MSA) connected in series with a second eluent generator producing potassium hydroxide (KOH). Through manipulating the concentration output of both eluent generators, chromatographic performance comparable to that obtained using the conventional sodium acetate/sodium hydroxide (NaOAc/NaOH) eluents is achieved using the electrolytically generated potassium methanesulfonate/potassium hydroxide (KMSA/KOH) eluent. This platform utilizes deionized water as the only carrier stream through a single isocratic pump, overcomes the various drawbacks associated with manually prepared NaOAc/NaOH eluents, and offers an easy to use, simplified operation solution for oligosaccharides profiling with increased precision and accuracy.

"Venha se você for homem": O princípio da masculinidade em orgias entre homens / "Come if you are a man": The principle of masculinity in orgies between men / "Llegue si eres hombre": El principio de la masculinidad en orgías entre hombres

Resumo: Neste artigo apresento uma reflexão sobre a masculinidade pensada e gestada entre os participantes de festas de orgia para homens na cidade do Rio de Janeiro. Com base em dados de campo de uma pesquisa etnográfica que desenvolvi ao acompanhar a organização e realização desses eventos foi possível perceber a elaboração de determinados princípios que dão conteúdo e expressão a essas festas. O primeiro deles é o da masculinidade. Apenas homens, e um determinado tipo de homem, podem participar desses eventos. Nesse artigo abordo essa figura do "macho", suas práticas, sua construção nesse contexto, seus paradoxos e tensões com outros elementos. Procuro tanto apresentar a produção daquilo que surge denominado como "caráter espartano", quanto problematizar diferentes formas de "habitar a norma" apresentadas nesses contextos.

Abstract: In this article, I present a reflection on masculinity thought and gestated among the participants of orgy parties for men in the city of Rio de Janeiro. Based on field data from an ethnographic research that I developed by accompanying the organization and realization of these events, it was possible to perceive the elaboration of certain principles that give content and expression to these parties. The first one is masculinity. Only men, and a certain type of man, can participate in these events. It is about this "macho" figure, its practices, its construction in this context, its paradoxes and tensions with other elements, which I intend to address in this article. I try both to present the production of what they call the "Spartan character" and to problematize different forms of "inhabit the norm" presented in these contexts.

Resumen: En este artículo presento una reflexión sobre la masculinidad pensada y gestada entre los participantes de fiestas de orgía para hombres en la ciudad de Río de Janeiro. Con base en datos de campo de una investigación etnográfica que desarrollé al acompañar la organización y realización de esos eventos, fue posible percibir la elaboración de determinados "principios" que dan contenido y marca pública a esas fiestas. El primero de ellos es el de la masculinidad. Sólo hombres, y un determinado tipo de hombre, pueden participar en estos eventos. Es sobre esa figura del "macho", sus prácticas, su construcción en ese contexto, sus paradojas y tensiones con otros elementos, que pretendo abordar en ese artículo. Busco tanto presentar la producción de lo que ellos llaman "carácter espartano", como problematizar diferentes formas de "habitar la norma" presentada en esos contextos.

Oligosaccharide analysis by capillary-scale high-pH anion-exchange chromatography with on-line ion-trap mass spectrometry.

A capillary-scale high-pH anion-exchange chromatography (HPAEC) system for the analysis of carbohydrates was developed, in combination with two parallel on-line detection methods of sub-picomolar sensitivity: (1) pulsed amperometric detection (PAD); (2) capillary-scale desalting followed by electrospray ion-trap (IT) mass spectrometry (MS). The capillary chromatographic system combined the superb selectivity of HPAEC that allows routine separation of isomeric oligosaccharides with the information on monosaccharide sequence and linkage positions obtained by MS/MS fragmentation using the IT-MS. The applicability of the system in biomedical research was demonstrated by its use for the analysis of a urine sample of a GM1-gangliosidosis patient. Isomeric glycans in the sample could be resolved by HPAEC and assigned on the basis of the monosaccharide linkage information revealed by on-line IT-MS/MS.

Control of oligonucleotide retention on a pH-stabilized strong anion exchange column.

Strong anion exchange columns are preferred for oligonucleotide analyses due to their ability to effectively control secondary structure and poly(G) interactions. Methacrylate-based anion exchange phases minimize hydrophobic interactions with oligonucleotides, but they also tend to hydrolyze under alkaline conditions. In this article, we report the use of an anion exchange column prepared from a new class of methacrylate monomers designed to improve hydrolytic stability. This column is used to show predictable adjustment of oligonucleotide retention by eluent pH and composition. Features of the new column include (i) large, predictable, pH-dependent retention shifts (varying with specific changes in 5' or 3' terminal bases with NaCl-based eluents); (ii) reduced retention when solvent is added to NaCl-based eluents; and (iii) suppression of much of the column's hydrophobic interactions when CH3CN is used with NaClO4-based eluents at a neutral pH (i.e., this eluent system separates oligonucleotides primarily in order of their length). These observations will aid the development of elution conditions for both size-dependent and base sequence-dependent (or base composition-dependent) separations.

CarboPac PA20: a new monosaccharide separator column with electrochemical detection with disposable gold electrodes.

This report documents the development of a new monosaccharide separator column (CarboPac PA20, 3x150 mm) that allows fast, efficient monosaccharide separations with good spacing. It is based on a new chemistry with a reduced resin particle size (from 10 to 6.5 microm). Faster, more efficient separations of glycoprotein monosaccharides with better spacing were achieved across a range of isocratic NaOH concentrations at lower flow rates. Detection sensitivity was improved, enabling routine low to sub pmol monosaccharide determinations. Glycoprotein monosaccharides eluted in less than 10 min at a flow rate of 0.5 ml/min. Furthermore, when used with an AminoTrap guard column, the protein matrix consisting of amino acids and peptides (released by acid hydrolysis of glycoprotein) did not interfere with monosaccharide analysis. Compared to previous CarboPac columns (CarboPac PA1 and CarboPac PA10), the CarboPac PA20 has improved selectivity with respect to glycoprotein monosaccharides. The improved selectivity results in better separation of glucosamine and galactose, enabling the accurate determination of monosaccharide ratios for undergalactosylated glycoproteins. Finally, disposable gold working electrodes that eliminate the possibility of working electrode recession affecting peak area response were used.

Determination of anions at trace levels in power plant water samples by ion chromatography with electrolytic eluent generation and suppression.

An ion chromatographic method was developed for the determination of nine inorganic and organic acid anions at sub- to low-microg/l levels in power plant water samples. In this method, samples were injected using a large-volume direct injection technique, the analyte anions were separated on a hydroxide-selective anion-exchange column using high-purity hydroxide eluents generated by an on-line electrolytic eluent generator and detected using the suppressed conductivity detection method. The method performance was evaluated by analyzing synthetic water samples containing additives encountered in the power plant water samples and four water samples from a fossil fuel power plant. The relative standard deviations of retention times of analyte ions separated on the hydroxide-selective anion-exchange column were less than 0.4%. The recoveries of analyte ions spiked into the synthetic water samples at concentrations of 0.13-1.0 microg/l were in the range of 70-120%. The method detection limits for analyte ions in deionized water were 0.0099, 0.0056, 0.019, 0.057, 0.0084, 0.023, 0.067, 0.037, and 0.079 microg/l for fluoride, acetate, formate, chloride, nitrite, sulfate, bromide, nitrate, and phosphate, respectively.

Exposición percutánea y de membranas mucosas en alumnos de odontología / Percutaneous injuries and mucous menbrane exposures among dental students

El propósito de este estudio fue determinar la frecuencia de lesiones percutáneas y salpicaduras y sus factores asociados en 205 estudiantes del 3er, 4to y 5to de estudios de odontología de la Universidad Peruana Cayetano Heredia. La información se obtuvo a través de fichas de auto-reporte de lesiones percutáneas y salpicaduras (eventos de riesgo de contaminación paciente-operador) ocurridos en la Clínica Estomatológica Central entre los meses de julio y octubre de 1995. Se reportaron 39 eventos (20 lesiones percutáneas y 19 salpicaduras), sobre un total de 4,516 procedimientos clínicos lo que arrojó un 1.2 por ciento de probabilidad que ocurra un evento durante un procedimiento clínico. El 14 por ciento de los alumnos reportaron por los menos un evento en los últimos cuatro meses. El número de eventos fue mayor en los alumnos de tercero y cuarto año que en aquellos del quinto año. La operatoria dental y la exodoncia fueron las actividades clínicas más relacionadas con lesiones percutáneas, la operatoria dental y la profilaxis fueron las actividades clínicas más relacionadas con exposición a salpicaduras. Se recomienda intensificar las medidas de control de infecciones para minimizar el riesgo de trasmisión ocupacional.