Transmission of haemotropic mycoplasma in the absence of arthropod vectors within a closed population of dogs on ectoparasiticides.

Dog-infecting haemotropic mycoplasmas (haemoplasmas), such as Mycoplasma haemocanis and Candidatus Mycoplasma haematoparvum are common blood-borne pathogens of canines that can potentially inflict a substantial burden of disease, particularly in immunosuppressed individuals. Nonetheless, the transmission of these pathogens remains debated as more evidence emerges that they may not be transmitted by vectors, but instead use alternative methods such as aggressive interactions and vertical transmission. Here, we treated forty dogs with two different topically-acting ectoparasiticide products able to prevent vector-borne pathogen infections during an 8-month community trial in Cambodia. A total absence of ectoparasites were observed at all time points, and no new infections caused by pathogens confirmed as being vectorially-transmitted were detected, i.e., Babesia vogeli, Ehrlichia canis, Anaplasma platys, and Hepatozoon canis. Conversely, the number of haemoplasma infections in dogs on both ectoparasiticides rose significantly, with an incidence of 26 infections per 100 dogs at risk per year, providing strong evidence of non-vectorial transmission. Over the study period, dog aggression and fighting were frequently observed, highlighting a different potential mode of transmission. This study presents the first robust evidence that canine haemoplasmas may be transmitted without arthropod vectors drawing attention to the need for new methods to prevent their transmission.

Field trial investigating the efficacy of a long-acting imidacloprid 10%/flumethrin 4.5% polymer matrix collar (Seresto®, Elanco) compared to monthly topical fipronil for the chemoprevention of canine tick-borne pathogens in Cambodia.

The tropical brown dog tick, Rhipicephalus linnaei, commonly infests canines in the tropics and is an important vector for disease-causing and sometimes lethal pathogens including Babesia spp., Ehrlichia canis, Hepatozoon canis and Anaplasma platys. In tropical climates ticks and their pathogens exert an extremely high infection pressure on unprotected dogs. To protect canines in such regions, effective acaricidal products possessing a speed of kill that blocks pathogen transmission is paramount. We conducted a 12-month community trial to compare the chemoprophylactic efficacy of two topical commercial acaricidal formulations: an imidacloprid 10% and flumethrin 4.5%, 8-month acting collar (Seresto®) against a monthly spot-on containing 12% w/v fipronil (Detick, Thailand). In a separate analysis, we used baseline data collected at the start of the trial to quantify tick-borne pathogen (TBP) infection status in dogs with a prior history of being administered a systemically-acting (isoxazoline) versus a topically-acting ectoparasiticide. We found that both topical products in the community trial demonstrated high efficacy at protecting dogs from ticks and TBP, with Seresto® demonstrating a moderate increase in protection at 3 (95% confidence interval (CI): 1-5) TBP-positive dogs per 100 dog-years at risk compared to 11 (95% CI: 4-26) TBP-positive dogs per 100 dog-years at risk for those on fipronil. Additionally, at baseline dogs treated with commercial systemic isoxazoline acaricides prior to the trial's commencement were 2.7 (95% CI: 0.5-15.0) times more likely to be TBP-positive compared to dogs that had been topically treated, highlighting such isoxazoline products as being less efficacious than topical products at preventing canine TBP acquisition in a tropical setting.