RESUMO
Curcumin-based novel colloidal nanocapsules were prepared from amphiphilic poly(ethylene oxide)-block-poly(propylene oxide)-block-poly(ethylene oxide) (F108). These colloidal nanocapsules appeared as spherical particles with size ranging between 270 and 310 nm. Curcumin fluorescence spectra exhibited an aggregation-induced 23 nm red-shift of the emission maximum in addition to the enhancement of the fluorescence quantum yield in these nanocapsules. The cytotoxicity of curcumin and colloidal nanocapsules was assessed using human derived immortalized cell lines (A549 and A375 cells) in the presence and absence of light irradiation. The nanocapsules exhibited a >30-fold decrease in IC50, suggesting enhanced anticancer activity associated with curcumin encapsulation. Higher toxicity was also reported in the presence of light irradiation (as shown by the IC50 data), indicating their potential for future application in photodynamic therapy. Finally, A375 cells treated with curcumin and the nanocapsules showed a significant increase in single- and/or double-strand DNA breaks upon exposure to light, indicating promising biological effects.
Assuntos
Antineoplásicos/farmacologia , Curcumina/farmacologia , Nanocápsulas/química , Fotoquimioterapia , Fármacos Fotossensibilizantes/farmacologia , Polietilenoglicóis/farmacologia , Propilenoglicóis/farmacologia , Tensoativos/farmacologia , Células A549 , Antineoplásicos/síntese química , Antineoplásicos/química , Proliferação de Células/efeitos dos fármacos , Coloides/síntese química , Coloides/química , Coloides/farmacologia , Curcumina/química , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Estrutura Molecular , Fármacos Fotossensibilizantes/síntese química , Fármacos Fotossensibilizantes/química , Polietilenoglicóis/química , Propilenoglicóis/química , Relação Estrutura-Atividade , Tensoativos/químicaRESUMO
In a novel approach, curcumin has been encapsulated inside Poly(Ethylene Oxide)-Block-Poly(Propylene Oxide)-Block-Poly(Ethylene Oxide) (F108) nanocapsules. FTIR spectra have indicated a type of hydrogen bonding and dipole interaction between curcumin and F108. Fluorescence and UV-visible absorption profiles of curcumin in nanocapsules have indicated location of curcumin in more hydrophobic microenvironment. The relative fluorescence yield has increased by 6 times in the nanocapsules, which renders them as more sensitive probes to be used later on in sensing study. Therefore, based on the functionality of curcumin as a fluorescent transducer, encapsulated curcumin is used in biomedical application as DNA and RNA sensing. Detection limits are detected as 50 µM and 60 µM for DNA and RNA respectively. Linear dynamic concentration range obtained in this proposed method is much higher than reported in literature. The interaction between the nanocapsules and targeted DNA/RNA molecules is further approved by zeta potential studies. Furthermore, the real interaction of DNA with the encapsulated curcumin is confirmed by the interaction of the adenine and cytosine nucleotides. This has been verified through zeta potential measurements. Moreover, our prepared nanocapsules has presented a high percentage recovery of DNA and RNA (96-101%). Finally, stability results have illustrated a high photostability of encapsulated curcumin, indicating that proposed nanocapsules can be considered as a stable sensor during measurement time.
Assuntos
Curcumina/química , DNA/análise , Corantes Fluorescentes/química , Nanocápsulas/química , Polietilenoglicóis/química , Propilenoglicóis/química , RNA/análise , Fluorescência , Interações Hidrofóbicas e Hidrofílicas , Tamanho da Partícula , Propriedades de SuperfícieRESUMO
New dual electrochemical and fluorescence sensitive curcumin-graphene quantum dots sensing platform coated on the transparent Indium-Tin-Oxide electrode was developed to sense APOe4 DNA, responsible of Alzheimer's disease. Curcumin molecule with its dual fluorescence and electrochemical properties was electropolymerized on GQDs-ITO surface. EDC/NHS chemistry was used to covalently immobilize an amino-substituted DNA probe via a malonic acid spacer. Quenching of curcumin signals following hybridized DNA complex was employed to quantify APOe4 DNA. Amperometric studies revealed an ultrasensitive behavior toward the formation of DNA complex with a sensitivity of 4.74â¯nA.mL.pg -1 and a limit of detection as low as 0.48â¯pgâ¯mL -1. The platform exhibits very good performances such as repeatability, reproducibility, selectivity and long storage stability. Fluorescence results were established for the support and the complementarity of electrochemical results. Founded results confirmed the ultrasensitivity of platform with comparable performances. Recorded results in human blood plasma demonstrated the high efficacy of curcumin system sensing even in the clinical matrix.