GC-MS analysis, phytochemical composition of Hertia cheirifolia L. essential oil with pharmacological assessments: antioxidant, antibacterial, and antifungal activities.

The genus Hertia, which belongs to the Asteraceae family, is a flowering genus with 12 species found in Africa, North and South. Among the species present in Algeria, Hertia cheirifolia L. is distributed in the eastern regions of Algeria. The aim of this study is to evaluate its phytochemical composition with following pharmacological assessments: the antioxidant, antibacterial, and antifungal activities of Hertia cheirifolia L. essential oil (EO). GC-MS analysis was used to analyze the chemical constituents of H. cheirifolia essential oil. The antioxidant capacity was assessed using DPPH, FRAP, and H2O2 tests. The EO was also tested for its ability to inhibit six strains of microorganisms, including two Gram (+) and four Gram (-) strains. The antifungal activity was tested by analyzing the effect of the EO on the mycelial growth of Fusarium oxysporum f.sp. lycopersici (FOL) fungi. Results showed that primary volatile components were α-pinene (32.59%), 2-(1-cyclopent-1-enyl-1-methylethyl) cyclopentanone (14.62%), (-)-germacrene D (11.37%), and bakkenolide A (9.57%). H. cheirifolia EO showed inhibitory effects against DPPH, H2O2, and FRAP (IC50 = 0.34 ± 0.1, 0.053 ± 0.1, and 0.047 ± 0.01 mg mL-1, respectively). The EO also exhibited moderate antibacterial effects against Staphylococcus aureus ATCC 25923 (S. aureus), Streptococcus pneumoniae ATCC 49619 (S. pneumoniae), and Enterobacter aerogenes ATCC 13048 (E. aerogenes), as well as significant antioxidant potential and varied antifungal activity based on dosage and fungal strain. To our knowledge, no previous research has examined the antifungal capacity of H. cheirifolia oil and oil-mycelial development of the FOL relationship. To fully explore the benefits of H. cheirifolia EO, more in vivo research is necessary, along with more testing on other bacterial and fungal strains.

Polyphenolic content and bioactivities of Crataegus oxyacantha L. (Rosaceae).

Solid-Liquid Extraction (SLE) using solvent of different polarities (CHCl3, EtOAc, and n-BuOH) has been applied to leaves and fruits from (Crataegus oxyacantha L.), a deciduous shrub with an expected rich phytochemical profile. The total polyphenols content and the radical scavenging activity of each extract were evaluated. These extracts were analyzed by HPLC-DAD and rutin, quercetin-3-glucoside, caftaric and caffeic acid had been positively identified. The phytochemical study of the ethyl acetate extract of C. oxyacantha, led to the isolation and structural elucidation of quercetin (1); quercetin-3-O-ß-glucoside (2); epicatechin (3); naringenin (4), reported for the first time from this species except caffeic acid and epicatechin. These compounds were identified by 1D and 2D NMR combined analysis as well as by MS and UV.The antimicrobial activity of these extracts has also been tested, showing strong antibacterial activity-solvent dependent-against Gram positive bacteria. Additionally, bactericidal power was demonstrated in fruit extracts.

Chemical constituents, in vitro antioxidant and antimicrobial properties of ethyl acetate extract obtained from Cytisus triflorus l'Her.

The present study investigates the phenolic composition, antioxidant and antimicrobial activities of an ethyl acetate extract from C. triflorus L'Her. The phytochemical study on the aerial parts of C. triflorus belonging to the Fabaceae family led to the isolation and structural elucidation of 5-Hydroxy-7-O-glucosylflavone (P1), 5-Hydroxy-7-O-galactosylflavone (P2), 5,7-Dihydroxy-flavone (P3), 5,7,3'-Trihydroxy,4'-methoxy-flavone (P4). These compounds were identified by 1D and 2D NMR combined analysis as well as by UV.Ethyl acetate extract of C. triflorus showed a significant and dose-dependent antioxidant activity in vitro, related to the presence of phenolics (180.33 ± 12.22 µg GAE/mg) and flavonoids (16.78 ± 1.54 µg QE/mg). The antimicrobial activity was evaluated in vitro against Staphylococcus aureus CECT 240 and Escherichia coli CECT 4099, by agar-diffusion method. The most active antibacterial activity was expressed by ethyl acetate extract of C. triflorus against Gram-positive bacteria S. aureus. The gathered results suggest that C. triflorus polyphenols and flavonoids are closely associated to its antioxidant and antimicrobial properties.

Hepatoprotective Effects of Algerian Crataegus oxyacantha Leaves.

BACKGROUND: Hawthorn (C. oxyacantha), a common edible plant, is widely used for the preparation of a different foodstuff and is also used in traditional medicine to treat heart problems and gastrointestinal ailments. Recently, a few patents of Crataegus preparation for protective effects (prevention of cardiovascular and hepatic diseases) have been developed. OBJECTIVE: The current study aimed to explore the antioxidant and hepatoprotective effects of nbutanol extract of Crataegus oxyacantha leaves in acute liver damage induced by Doxorubicin (DOX). METHODS: Crataegus oxyacantha (100 mg/kg body weight) or vitamin E as a standard antioxidant (100 mg/kg body weight) were administered orally to female rats for 10 days, in the presence or absence of hepatotoxicity induced by a single intraperitoneal (i.p.) injection of DOX (15 mg/kg on the 8th day). On day 11, blood and liver samples were analyzed for biomarker levels and histopathological changes. Liver homogenates were used for determination of oxidative stress parameters that include Malondialdehyde (MDA), Glutathione (GSH) level and Glutathione Peroxidase (GPx) activity. RESULTS: Treatment with n-butanol extract of C. oxyacantha leaves significantly improved the altered liver enzyme activities and oxidative stress markers. The histopathological observations confirm the results of biochemical parameters. CONCLUSION: The obtained results support the traditional use of C. oxyacantha to cure gastrointestinal ailments and highlighted its possible use in the food and pharmaceutical industries as a source of natural antioxidant.

Matrix solid-phase dispersion as a tool for phytochemical and bioactivities characterisation: Crataegus oxyacantha L._A case study.

The use of a matrix solid-phase dispersion (MSPD) process to extract polyphenols from hawthorn (Crataegus oxyacantha L.) a deciduous shrub with an expected rich phytochemical profile, has been evaluated. MSPD extracts of fruits and leaves have an outstanding content of polyphenols, although the particular phenolic profile is solvent dependent. The extracts were analysed by HPLC-DAD for the accurate identification of the major bioactive polyphenols, some of which have never been described for this species. MSPD has proven to be a good alternative to the classic methods of obtaining natural extracts, fast and with low consumption of organic solvents, therefore, environmentally friendly. The bioactivities can be considered also very remarkable, revealing extracts with high levels of antioxidant activity.