RESUMO
The article presents data concerning analysis of bioassays under suspicion of influenza A(H1N1)pdm09. The technique of back transcription polymerase chain reaction and sequencing was applied. The separation of native influenza virus A was implemented using passaging through developing chick embryos.
Assuntos
Hemaglutininas Virais/genética , Vírus da Influenza A Subtipo H1N1/genética , Influenza Humana/virologia , RNA Viral/isolamento & purificação , Animais , Embrião de Galinha , Hemaglutininas Virais/isolamento & purificação , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Vírus da Influenza A Subtipo H1N1/isolamento & purificação , Vírus da Influenza A Subtipo H1N1/patogenicidade , Vírus da Influenza A Subtipo H1N1/ultraestrutura , Influenza Humana/genética , Influenza Humana/patologia , Microscopia Eletrônica , RNA Viral/genéticaRESUMO
The virological, morphological, molecular biological and immunochemical study of the infective agent isolated from the patient with the symptoms of atypical pneumonia, hospitalized in the infectious department of the clinical hospital in Blagoveshchensk, was carried out. Thus the fact of the appearance of SARS virus on the territory of Russia was proved. The isolated infective agent, identified as coronavirus strain CoD, was partly characterized and deposited to the virus collection of the Center of Special Laboratory Diagnostics and Treatment of Quarantine and Exotic Infectious Diseases.
Assuntos
Pneumonia Viral , Síndrome Respiratória Aguda Grave/virologia , Coronavírus Relacionado à Síndrome Respiratória Aguda Grave/isolamento & purificação , Animais , Chlorocebus aethiops , Efeito Citopatogênico Viral , Humanos , Masculino , RNA Viral/análise , RNA Viral/sangue , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Coronavírus Relacionado à Síndrome Respiratória Aguda Grave/genética , Coronavírus Relacionado à Síndrome Respiratória Aguda Grave/imunologia , Coronavírus Relacionado à Síndrome Respiratória Aguda Grave/ultraestrutura , Síndrome Respiratória Aguda Grave/diagnóstico , Síndrome Respiratória Aguda Grave/patologia , Células VeroRESUMO
The comparative study of the specimens of the morphological elements of exanthema obtained from 8 children with the clinical diagnosis of secondary exogenic vaccinia, dried smallpox vaccine and the cultures of other orthopoxviruses (rabbit pox, monkey pox and buffalo pox viruses) was made. The isolation and identification of the causative agents from the specimens was carried out with the use of modern virological, electron microscopic and molecular methods. The study proved the fact that 8 children had orthopoxvirus infection with its causative agent identified as vaccinia virus.
Assuntos
Vacínia/virologia , Vírus da Varíola/isolamento & purificação , Criança , Feminino , Humanos , Doença Iatrogênica , Masculino , Microscopia Eletrônica , Reação em Cadeia da Polimerase , Vacina Antivariólica , Vacínia/diagnóstico , Vírus da Varíola/genéticaRESUMO
The results of the isolation and identification of the causative agent of a haemorrhagic fever outbreak in the Stavropol Territory are presented. The virus isolated from blood of haemorrhagic fever patients by virological methods was identified in serological and molecular tests as Crimean haemorrhagic fever virus. This epidemiological analysis testify to increased activity of the natural focus of Crimean-Congo haemorrhagic fever in this area due to a number of natural and other factors leading to intensification of its epidemic realization.
Assuntos
Surtos de Doenças , Vírus da Febre Hemorrágica da Crimeia-Congo/classificação , Vírus da Febre Hemorrágica da Crimeia-Congo/isolamento & purificação , Febre Hemorrágica da Crimeia/epidemiologia , Animais , Animais Lactentes , Chlorocebus aethiops , Vetores de Doenças , Genótipo , Vírus da Febre Hemorrágica da Crimeia-Congo/genética , Febre Hemorrágica da Crimeia/virologia , Humanos , Camundongos , Nucleoproteínas/genética , Reação em Cadeia da Polimerase , Federação Russa/epidemiologia , Sorotipagem , Carrapatos/virologia , Células Vero , Proteínas Virais/genéticaRESUMO
Experiments were conducted in vitro. A study was made of the action of the enzymes and of the secretions of the gastrointestinal tract on the viability of the EB vaccine strain. Sensitivity of bacterial cells to the action of saliva, gastric juice, intestinal contents, trypsin, and bile proved to differ. Gastric juice inactivated the vaccine strain which expressed a satisfactory resistance towards the rest of the factors under study practically immediately. There were revealed no significant morphological changes in the cells subjected to the two-hour action of saliva, bile, trypsin and the intestinal content, by means of the electron microscopic examination. These studies permitted to substantiate the rational form of the preparation for oral administration.