RESUMO
Due to unique surface chemistries and the ability to easily functionalize their surface, amorphous silica nanoparticles are being assimilated into medicinal and consumer products at an increasing rate. Subsequently, there is an emergent need to understand the interactions of these particulates with biological systems in an attempt to mitigate toxicity. The identification of susceptible or resistant cell types of the pulmonary system remains a critical step in the development of toxicity assessments for nanoparticle-based platforms. Specific to this study, the cellular responses of A549 lung epithelial and MeT-5A pleural mesothelial cell lines as a means of detecting nanoparticle-induced oxidative stress were examined. Basal expression and cellular antioxidant activity, including SOD, CAT, and GSH, were examined prior to H(2)O(2) and ~30 nm SiO(2) (0.01-100mg/L) exposures. Dose-response observations were made regarding oxidant production, cytotoxicity, GSH depletion and NRF2 transcription factor activation. Results indicated that, while both cell types exhibited susceptibility to H(2)O(2) and SiO(2)-induced oxidative stress and damage, the A549 cell line was relatively more resilient.
Assuntos
Células Epiteliais/efeitos dos fármacos , Nanopartículas/toxicidade , Dióxido de Silício/toxicidade , Catalase/metabolismo , Linhagem Celular , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Células Epiteliais/metabolismo , Epitélio , Glutationa/metabolismo , Humanos , Pulmão/citologia , Fator 2 Relacionado a NF-E2/metabolismo , Superóxido Dismutase/metabolismoRESUMO
The immunological effects of quantum dots are dependent on a variety of factors including, but not limited to, exposure time and dosing concentrations. In this study, we investigated the influence of 15 nm CdSe/ZnS-COOH quantum dot nanocrystals (QDs) on cell density, viability, and morphology in human epidermal keratinocytes (HEK) and human dermal fibroblasts (HDF). Furthermore, inflammatory and non-inflammatory immune responses were measured using protein and real time PCR array analysis from HDF cells exposed to predetermined sub-lethal concentrations of QDs. CdSe/ZnS-COOH QDs caused concentration-dependent (1-120 nM exposure concentrations) and time-dependent (8 h or 48 h) cell death, as evidenced by metabolic activity and morphological changes. QD exposure induced upregulation of apoptotic, inflammatory and immunoregulatory proteins such as TNF-α, IL-1B and IL-10. HMOX1, an indicator of stress due to reactive oxygen intermediates (ROIs) and/or metals, was upregulated at the later time point as well. QDs also caused modulation of genes known to be associated with inflammatory (IL1-ß, CCL2, IRAK-2), immune (IL-1, IL-6, PGLYRP1, SERPINA1, IL-10), stress due to ROIs and/or heavy metals (HMOX1), and apoptotic (CASP1, ADORA2A) responses. Cellular effects from QD exposure were found to primarily follow the NFκB pathway. In addition, QDs induced a differential cytotoxicity in keratinocytes and fibroblasts at different exposure concentrations and time points, even at physiologically relevant dosing concentrations, thus emphasizing the need to investigate potential mechanisms of action among different cell types within the same target organ.
Assuntos
Fibroblastos/imunologia , Queratinócitos/imunologia , NF-kappa B/metabolismo , Pontos Quânticos , Apoptose , Compostos de Cádmio/imunologia , Morte Celular , Citocinas/biossíntese , Fibroblastos/citologia , Fibroblastos/metabolismo , Expressão Gênica , Heme Oxigenase-1/biossíntese , Humanos , Imunomodulação , Inflamação/imunologia , Queratinócitos/citologia , Queratinócitos/metabolismo , Metais/imunologia , Reação em Cadeia da Polimerase , Espécies Reativas de Oxigênio/imunologia , Espécies Reativas de Oxigênio/metabolismo , Compostos de Selênio/imunologia , Pele , Sulfetos/imunologia , Compostos de Zinco/imunologiaRESUMO
The risk of potential human exposure to mixed nanomaterials in consumer, occupational, and medicinal settings is increasing as nanomaterials enter both the workplace and the marketplace. In this study, we investigated the toxicity of mixed engineered carbon black (ECB) and maghemite iron oxide (Fe(2)O(3)) nanoparticles in a cellular system to understand the mechanism of toxicity and potential methods of toxicity mitigation. Lung epithelial cells (A549) were exposed to mixed Fe(2)O(3) and ECB nanoparticles, mixed Fe(2)O(3) and ECB nanoparticles with the addition of L-ascorbic acid, and mixed Fe(2)O(3) and surface-oxidized engineered carbon black (ox-ECB) nanoparticles. The nanoparticles were characterized using transmission electron microscopy, nitrogen adsorption surface area measurement (BET), X-ray diffraction, and surface charge measurement. The carbon black nanoparticles were also characterized with a reductive capacity assay and by X-ray photoelectron spectroscopy (XPS). The cellular uptake of nanoparticles was analyzed via transmission electron microscopy and fluorescence microscopy; the cellular uptake of iron was quantified using inductively coupled plasma mass spectrometry (ICP-MS). Both the MTT assay and the ethidium homodimer and calcein AM live/dead assay were used to measure cellular proliferation and cytotoxicity, respectively. The dichlorofluorescein diacetate (DCFH-DA) assay was used to measure the intracellular generation of reactive oxygen species. Results show that both Fe(2)O(3) and ECB (or Fe(2)O(3) and ox-ECB) were co-internalized in intracellular vesicles. Additionally, after exposure to the mixture of nanoparticles, the amount of acidified lysosomes increased over time. The cellular uptake of Fe(2)O(3) nanoparticles was unaffected by mixing with ECB. Significant oxidant production occurred in cells exposed to mixed Fe(2)O(3) and ECB, but not in cells exposed to mixed Fe(2)O(3) and ox-ECB or in cells exposed to Fe(2)O(3) and ECB with the addition of ascorbic acid. Furthermore, exposure to mixed Fe2O3 and ECB nanoparticles yielded a dose-dependent decrease in the level of cellular proliferation (MTT assay) and a decrease in cellular viability (ethidium homodimer and calcein AM live/dead assay) that were not seen in the Fe(2)O(3) and ox-ECB scenario. The results support the hypothesis that exposure to mixed Fe(2)O(3) and ECB nanoparticles produces oxidants that are mediated by the surface reductive capability of ECB when both particle types are colocalized in acidic cellular compartments. This oxidant production mechanism may lead to oxidative stress, but it can be mitigated by an antioxidant such as ascorbic acid or by surface treatment of the ECB to decrease its surface reductive capacity.