Exploring the Potential Therapeutic Approach Using Ginsenosides for the Management of Neurodegenerative Disorders.

There is a need for an efficient and long-lasting treatment due to the population's increasing prevalence of neurodegenerative disorders. In an effort to generate fresh ideas and create novel therapeutic medications, scientists have recently started to investigate the biological functions of compounds derived from plants and herbs. Ginseng, famous Chinese herbal medicine, has therapeutic value by virtue of its compounds ginsenosides or panaxosides, which are triterpene saponins and steroid glycosides. Research revealed positive impacts on ameliorating various disease conditions and found it as a possible drug candidate. Several neuroprotection mechanisms followed by this compound are inhibition of cell apoptosis, oxidative stress, inflammatory, and tumor activity. It has been demonstrated that controlling these mechanisms enhances cognitive performance and safeguards the brain against neurodegenerative disorders. The main objective of this review is to give a description of the most recent studies on ginsenoside's possible therapeutic application in the treatment of neurodegenerative diseases. Using organic compounds like ginseng and its various components may create new avenues for innovative treatment approaches development for neurological diseases. However, further research is necessary to confirm the stability and effectiveness of ginsenosides for neurodegenerative disease.

A comprehensive review on algal nutraceuticals as prospective therapeutic agent for different diseases.

Ongoing research in the food supplement sector provides insightful information regarding algae as a new-generation nutritional supplement and is also referred to as a superfood. Due to the diverse nutritional components, algae have documented numerous health benefits like fighting microbial diseases, hypertension, obesity, and diabetes. Therefore, algae-derived nutraceuticals account for a rapidly expanding market in the food supplements sector. The concept of algal prebiotics and their role in modulating gut microbiota have also been a chief contributor to this. This review evaluates the use of possible algal species and their specific bioactive compounds for the management of several chronic diseases. Proteins, peptides, polysaccharides, phenolics, and vitamins give an insight into the significance of algae in boosting the immune system and improving the body's nutritional makeup. In addition, phyco-compounds such as polysaccharides and polyphenols are also receiving a lot more interest in cosmeceutical applications for protecting skin from photodamage. The incorporation of algae in the diet for the management and prevention of chronic diseases like cancer, lung, and heart disease has been discussed in this review along with their action mechanism. This review provides a brief overview of several bioactive compounds present in micro and macroalgae and their therapeutic effect on lifestyle diseases, gastrointestinal diseases as well as neurodegenerative diseases.

Nano-inspired smart medicines targeting brain cancer: diagnosis and treatment.

Cancer, despite being the bull's eye for the research community, accounts for a large number of morbidity and mortality. Cancer of the brain is considered the most intractable, with the least diagnosis rates, hence treatment and survival. Despite the extensive development of therapeutic molecules, their targeting to the diseased site is a challenge. Specially tailored nanoparticles can efficiently deliver drugs and genes to the brain to treat tumours and diseases. These nanotechnology-based strategies target the blood-brain barrier, the local space, or a specific cell type. These nanoparticles are preferred over other forms of targeted drug delivery due to the chances for controlled delivery of therapeutic cargo to the intended receptor. Targeted cancer therapy involves using specific receptor-blocking compounds that block the spreading or growth of cancerous cells. This review presents an account of the recent applications of nano-based cancer theragnostic, which deal in conjunct functionalities of nanoparticles for effective diagnosis and treatment of cancer. It commences with an introduction to tumours of the brain and their grades, followed by hurdles in its conventional diagnosis and treatment. The characteristic mechanism of nanoparticles for efficiently tracing brain tumour grade and delivery of therapeutic genes or drugs has been summarised. Nanocarriers like liposomes have been widely used and commercialized for human brain cancer treatment. However, nano-inspired structures await their translational recognition. The green synthesis of nanomaterials and their advantages have been discussed. The article highlights the challenges in the nano-modulation of brain cancer and its future outlook.

Exploring different computational approaches for effective diagnosis of breast cancer.

Breast cancer has been identified as one among the top causes of female death worldwide. According to recent research, earlier detection plays an important role toward fortunate medicaments and thus, decreasing the mortality rate due to breast cancer among females. This review provides a fleeting summary involving traditional diagnostic procedures from the past and today, and also modern computational tools that have greatly aided in the identification of breast cancer. Computational techniques involving different algorithms such as Support vector machines, deep learning techniques and robotics are popular among the academicians for detection of breast cancer. They discovered that Convolutional neural network was a common option for categorization among such approaches. Deep learning techniques are evaluated using performance indicators such as accuracy, sensitivity, specificity, or measure. Furthermore, molecular docking, homology modeling and Molecular dynamics Simulation gives a road map for future discussions about developing improved early detection approaches that holds greater potential in increasing the survival rate of cancer patients. The different computational techniques can be a new dominion among researchers and combating the challenges associated with breast cancer.

Natural polyphenols: a promising bioactive compounds for skin care and cosmetics.

The physiological and morphological aspects of skin suffer from frequent change. Numerous internal and external factors have direct impact on inducing various skin problems like inflammation, aging, cancer, oxidative stress, hyperpigmentation etc. The use of plant polyphenols as a photo-ecting agent is gaining popularity nowadays. Polyphenols are known to enhance endogenic antioxidant system of skin thereby preventing various skin diseases. The biological activity of plant polyphenols is dependent on their physicochemical properties for overcoming the epidermal barriers to reach the specific receptor. Several evidences have reported the vital role polyphenols in mitigating adverse skin problems and reverting back the healthy skin condition. The interest in plant derived skin care products is emerging due to the changing notion of people to shift their focus towards use of plant-based products. The present review draws an attention to uncover the protective role of polyphenols in prevention of various skin problems. Several in vitro and in vivo studies have been summarized that claims the efficacious nature of plant extract having dermatological significance.

Chlorella minutissima-assisted silver nanoparticles synthesis and evaluation of its antibacterial activity.

The conventional methods of nanoparticles synthesis led to the production of highly toxic by-products and the use of toxic chemicals that are highly expensive in nature. Thus, the recent past has witnessed a surge in green synthesis of nanoparticles as a sustainable alternative. The present study outlines the biogenic silver nanoparticles (Ag-NPs) synthesis from an aqueous extract of Chlorella minutissima. The effect of certain parameters such as the reaction mixture's pH and precursor metal solution to algal extract ratios were explored and optimized. The UV spectrophotometric analysis of Ag-NPs gave surface plasmon response maximally at 426 nm. The developed Ag-NPs were characterized using zeta potential, indicating their high stability (-21.2 mV) with a mean diameter of 73.13 nm. Results from field emission-scanning electron microscopy (FE-SEM) showed that the particles were spherical in shape. Ag-NPs synthesized using Chlorella minutissima extract could significantly inhibit the growth of both Gram-positive and Gram-negative bacterial species. The study highlights that using C. minutissima extract for Ag-NPs synthesis is a convenient and fast process for controlling the growth of Gram-positive as well as Gram-negative bacteria.

Algal bioplastics: current market trends and technical aspects.

Abstract: Plastics are undebatably a hot topic of discussion across international forums due to their huge ecological footprint. The onset of COVID-19 pandemic has exacerbated the issue in an irreversible manner. Bioplastics produced from renewable sources are a result of lookout for sustainable alternatives. Replacing a ton of synthetic plastics with biobased ones reduces 1.8 tons CO2 emissions. Here, we begin with highlighting the problem statement-Plastic accumulation and its associated negative impacts. Microalgae outperforms plants and microbes, when used to produce bioplastic due to superior growth rate, non-competitive nature to food, and simultaneous wastewater remediation. They have minimal nutrient requirements and less dependency on climatic conditions for cultivation. These are the reasons for current boom in the algal bioplastic market. However, it is still not at par in price with the petroleum-based plastics. A brief market research has been done to better evaluate the current global status and future scope of algal bioplastics. The objective of this review is to propose possible solutions to resolve the challenges in scale up of bioplastic industry. Various bioplastic production technologies have been comprehensively discussed along with their optimization strategies. Overall studies discussed show that in order to make it cost competitive adopting a multi-dimensional approach like algal biorefinery is the best way out. A holistic comparison of any bio-based alternative with its conventional counterpart is imperative to assess its impact upon commercialization. Therefore, the review concludes with the life cycle assessment of bioplastics and measures to improve their inclusivity in a circular economy.

Disease Prevention and Treatment Using ß-Carotene: the Ultimate Provitamin A.

Humans being unable to synthesize beta-carotene, the provitamin A, depend on external sources as its supplement. Health benefits and dietary requirements of beta-carotene are interrelated. This orange-red coloured pigment has been enormously examined for its capacity to alleviate several chronic diseases including various types of cancer, cystic fibrosis, as well as COVID-19. However, this class of phytoconstituents has witnessed a broad research gap due to several twin conclusions that have been reported. Natural sources for these compounds along with their extraction methods have been mentioned. The current communication aims at contributing to the global scientific literature on beta-carotene's application in prevention and treatment of lifestyle diseases.

Potential Benefits of Nutraceuticals for Oxidative Stress Management.

Oxidative stress is an unbalanced redox state caused due to high concentration of reactive species and comparatively a very lower concentration of endogenous antioxidants in the body. When established, oxidative stress can disrupt cell structure and protein conformation and even damage genetic materials. Considering the degree of damage that oxidative stress can cause and the lack of promising treatment, preventing its onset is the best possible solution. Nutraceuticals, food or their extract that provides an added health benefit along with nutrition, can be used to prevent oxidative stress. An entire class of nutraceuticals are known for their inherent antioxidant properties, which can be included in our daily diet to prevent the occurrence of oxidative stress. Regulatory consumption of nutraceuticals can maintain a stable redox state, and thus prevents oxidative stress. In addition to managing oxidative stress, nutraceuticals can also help to control conditions like diabetes, neurodegeneration, cancer, organ inflammation, cardiovascular diseases, and other such conditions which are caused due to cellular oxidation. This review highlights the potential nutraceutical effect of several food and supplements which with further research along with genetic analysis can pave the road for nutrigenomics. Supplementary Information: The online version contains supplementary material available at 10.1007/s43450-022-00246-w.

Medicinal Plants for Glioblastoma Treatment.

Glioblastoma, an aggressive brain cancer, demonstrates the least life expectancy among all brain cancers. Because of the regulation of diverse signaling pathways in cancers, the chemotherapeutic approaches used to suppress their multiplication and spread are restricted. Sensitivity towards chemotherapeutic agents has been developed because of the pathological and drug-evading abilities of these diverse mechanisms. As a result, the identification and exploration of strategies or treatments, which can overcome such refractory obstacles to improve glioblastoma response to treatment as well as recovery, is essential. Medicinal herbs contain a wide variety of bioactive compounds, which could trigger aggressive brain cancers, regulate their anti-cancer mechanisms and immune responses to assist in cancer elimination, and cause cell death. Numerous tumor-causing proteins, which facilitate invasion as well as metastasis of cancer, tolerance of chemotherapies, and angiogenesis, are also inhibited by these phytochemicals. Such herbs remain valuable for glioblastoma prevention and its incidence by effectively being used as anti-glioma therapies. This review thus presents the latest findings on medicinal plants using which the extracts or bioactive components are being used against glioblastoma, their mechanism of functioning, pharmacological description, and recent clinical studies conducted on them.

Treasuring the computational approach in medicinal plant research.

Medicinal plants serve as a valuable source of secondary metabolites since time immemorial. Computational Research in 21st century is giving more attention to medicinal plants for new drug design as pharmacological screening of bioactive compound was time consuming and expensive. Computational methods such as Molecular Docking, Molecular Dynamic Simulation and Artificial intelligence are significant Insilico tools in medicinal plant research. Molecular docking approach exploits the mechanism of potential phytochemicals into the target active site to elucidate its interactions and biological therapeutic properties. MD simulation illuminates the dynamic behavior of biomolecules at atomic level with fine quality representation of biomolecules. Dramatical advancement in computer science is illustrating the biological mechanism via these tools in different diseases treatment. The advancement comprises speed, the system configuration, and other software upgradation to insights into the structural explanation and optimization of biomolecules. A probable shift from simulation to artificial intelligence has in fact accelerated the art of scientific study to a sky high. The most upgraded algorithm in artificial intelligence such as Artificial Neural Networks, Deep Neural Networks, Neuro-fuzzy Logic has provided a wide opportunity in easing the time required in classical experimental strategy. The notable progress in computer science technology has paved a pathway for understanding the pharmacological functions and creating a roadmap for drug design and development and other achievement in the field of medicinal plants research. This review focus on the development and overview in computational research moving from static molecular docking method to a range of dynamic simulation and an advanced artificial intelligence such as machine learning.

Current Prospects of Nutraceuticals: A Review.

Nutraceuticals are dietary supplements, utilized to ameliorate health, delay senescence, prevent diseases, and support the proper functioning of the human body. Currently, nutraceuticals are gaining substantial attention due to nutrition and therapeutic potentials. Based on their sources, they are categorized as dietary supplements and herbal bioactive compounds. The global market for nutraceutical is huge i.e. approximately USD 117 billion. Herbal nutraceutical helps in maintaining health and promoting optimal health, longevity, and quality of life. Studies have shown promising results of nutraceuticals to treat several diseases, such as cancer, neurodegenerative diseases, cardiovascular diseases, etc. In the present review, an overview of various bioactive ingredients that act as nutraceuticals (carbohydrates, lipids, edible flowers, alkaloids, medicinal plants, etc.) and their role in health benefits, has been discussed. Further application of nutraceuticals in the prevention of various diseases has also been discussed.

Market Analysis of Medicinal Plants in India.

There has been significant growth in the herbal drugs market around the world, over the last few decades due to growing awareness among people about the rising cost as well as side effects related to the use of synthetic drugs. Herbal medicine has been used in traditional medicinal systems around the world, especially India where the oldest systems of medicine namely Ayurveda, Siddha, and Unani make use of more than 90% plant-based formulations. India is rich in medicinal and herbal plants resources that provide for both the health care needs of rural India as well as the source of raw material for therapeutic agents required in the production of traditional and modern medicine. This review article presents an overview of the knowledge of Indian herbal plants based medicine in the national and international market and the trends in its production, sustainability, and promotion.

Molecular characterization, modeling, and docking analysis of late phytic acid biosynthesis pathway gene, inositol polyphosphate 6-/3-/5-kinase, a potential candidate for developing low phytate crops.

The coding sequence of inositol polyphosphate 6-/3-/5-kinase (GmIPK2) gene was identified and cloned from popular Indian soybean cultivar Pusa-16. The clone was predicted to encode 279 amino acids long, 30.97 kDa protein. Multiple sequence alignment revealed an inositol phosphate-binding motif, PxxxDxKxG throughout the IPK2 sequences along with other motifs unique to inositol phosphate kinase superfamily. Eight α-helices and eight ß-strands in antiparallel ß-sheets arrangement were predicted in the secondary structure of GmIPK2. The temporal analysis of GmIPK2 revealed maximum expression in the seed tissues during later stages of development while spatially the transcript levels were lowest in leaf and stem tissues. Endosperm-specific cis-regulatory motifs (GCN4 and Skn_1) which support high levels of expression, as observed in the developing seeds, were detected in its promoter region. The protein structure of GmIPK2 was modeled based on the crystal structure of inositol polyphosphate multikinase from Arabidopsis thaliana (PDB:4FRF) and subsequently docked with inositol phosphate ligands (PDB: 5GUG-I3P and PDB: 4A69-I0P). Molecular dynamics (MD) simulation established the structural stability of both, modeled enzyme and ligand-bound complexes. Docking in combination with trajectory analysis for 50 ns MD run confirmed the participation of Lys105, Lys126 and Arg153 residues in the formation of a network of hydrogen bonds to stabilize the ligand-receptor interaction. Results of the present study thus provide valuable information on structural and functional aspects of GmIPK2 which shall assist in strategizing our long-term goal of achieving phytic acid reduction in soybean by genetic modification of its biosynthetic pathway to develop a nutritionally enhanced crop in the future.

Development and Evaluation of Low Phytic Acid Soybean by siRNA Triggered Seed Specific Silencing of Inositol Polyphosphate 6-/3-/5-Kinase Gene.

Soybean is one of the leading oilseed crop in the world and is showing a remarkable surge in its utilization in formulating animal feeds and supplements. Its dietary consumption, however, is incongruent with its existing industrial demand due to the presence of anti-nutritional factors in sufficiently large amounts. Phytic acid in particular raises concern as it causes a concomitant loss of indigestible complexed minerals and charged proteins in the waste and results in reduced mineral bioavailability in both livestock and humans. Reducing the seed phytate level thus seems indispensable to overcome the nutritional menace associated with soy grain consumption. In order to conceive our objective we designed and expressed a inositol polyphosphate 6-/3-/5-kinase gene-specific RNAi construct in the seeds of Pusa-16 soybean cultivar. We subsequently conducted a genotypic, phenotypic and biochemical analysis of the developed putative transgenic populations and found very low phytic acid levels, moderate accumulation of inorganic phosphate and elevated mineral content in some lines. These low phytic acid lines did not show any reduction in seedling emergence and displayed an overall good agronomic performance.

Biotechnological Approaches for the Production of Pharmaceutically Important Compound: Plumbagin.

BACKGROUND: Increased demand for compounds that are derived from natural source are gaining more and more importance. Plumbagin is a plant naphthoquinone which is present in several families, including Iridaceae, Plumbaginaceae, Ebenceae, Drosophyllaceae, Nepenthaceae and Droseraceae. Plumbagin possesses high therapeutic efficacy and minimal side effects. It has various pharmaceutical activities which include anticancer, antibacterial, anti-inflammatory, antioxidant, antifungal, neuroprotective and hypolipidemic activities. In natural habitat, production of plumbagin is low due to species variations and environmental changes, considering importance of this bioactive compound, alternative techniques for its enhanced production needs to be devised. In the present review, various production techniques and scale-up strategies for plumbagin production are discussed. OBJECTIVES: Aim of this review is to provide an insight into the chemistry of plumbagin, its pharmaceutical activities, perspective of cell suspension culture, root culture, hairy root culture and scale up strategies for its production. METHODOLOGY: All the data compiled and presented here were obtained from various E-resources like Pubmed, Science Direct, and Google Scholar up to February 2018. RESULT: This review comprises isolation, extraction and quantification method for plumbagin, its pharmaceutical activities, various tissue culture production techniques and scale-up strategies for enhanced production. CONCLUSION: Plumbagin is an important phytocompound which shows potential towards treatments of various diseases. Demand for the production of plumbagin continuously increasing worldwide due to its pharmacological properties. To fulfil commercial demand of plumbagin alternative technologies need to be investigated. Biotechnological approaches like cell suspension culture, root suspension culture and hairy root culture are alternative techniques for plumbagin production. These techniques provide continuous supply of bioactive compounds. However, research on various aspects of tissue culture production techniques is in preparatory stage and requires culture and process optimization for development of a commercially practical process.

Host-mediated RNAi of a Notch-like receptor gene in Meloidogyne incognita induces nematode resistance.

GLP-1 (abnormal germline proliferation) is a Notch-like receptor protein that plays an essential role in pharyngeal development. In this study, an orthologue of Caenorhabditis elegans glp-1 was identified in Meloidogyne incognita. A computational analysis revealed that the orthologue contained almost all the domains present in the C. elegans gene: specifically, the LIN-12/Notch repeat, the ankyrin repeat, a transmembrane domain and different ligand-binding motifs were present in orthologue, but the epidermal growth factor-like motif was not observed. An expression analysis showed differential expression of glp-1 throughout the life cycle of M. incognita, with relatively higher expression in the egg stage. To evaluate the silencing efficacy of Mi-glp-1, transgenic Arabidopsis plants carrying double-stranded RNA constructs of glp-1 were generated, and infection of these plants with M. incognita resulted in a 47-50% reduction in the numbers of galls, females and egg masses. Females obtained from the transgenic RNAi lines exhibited 40-60% reductions in the transcript levels of the targeted glp-1 gene compared with females isolated from the control plants. Second-generation juveniles (J2s), which were descendants of the infected females from the transgenic lines, showed aberrant phenotypes. These J2s exhibited a significant decrease in the overall distance from the stylet to the metacorpus region, and this effect was accompanied by disruption around the metacorporeal bulb of the pharynx. The present study suggests a role for this gene in organ (pharynx) development during embryogenesis in M. incognita and its potential use as a target in the management of nematode infestations in plants.

Swertia chirata: A Comprehensive Review with Recent Advances.

BACKGROUND: Swertia chirata, an ethnomedicinal plant, is renowned for its diverse medicinal properties. The plant produces large number of therapeutic phytochemicals responsible for remedial effect against various ailments mainly chronic fever, malaria, bronchial asthma, liver disorders, certain type of mental disorder, blood purification and diabetes. Recently reported anticancer effect of S. chirata has amplified its importance in the scientific community. OBJECTIVE: The aim of this review is to deliver the pharmaceutical importance of S. chirata as diverse therapeutic agent and attract the scientific community working in the field of pharmacology to produce new drug to treat various ailments. METHODOLOGY: Data compiled and presented here were obtained from E-resources like Science Direct, Pubmed, Google, through books and web of science up to Oct 2017. RESULT: This review comprises the common aspects of S. chirata like pharmacologically important phytochemicals with in vitro and in vivo studies, toxicology, adulteration with similar species, dosage, conservation via in vitro studies and genetic stability with molecular markers. A bioinformatics approach to explore therapeutic targets that might suppress many diseases is also compiled to make it complete. CONCLUSION: The medicinal plant S. chirata is continuously being used as a traditional herb. The data indicated its safe and positive effect in the treatment of various diseases. It presents many promising prospects for modern medicine, which may be validated after the process of successful in vivo research, clinical studies, and human trials.

Uptake of Heavy Metals from Industrial Wastewater Using In Vitro Plant Cultures.

The plant species Bacopa monnieri has been observed to reduce the heavy metal concentrations in its vicinity. The present study is a comparison of in vitro culture and soil-grown plants of B. monnieri to remove Cr and Cd, from synthetic solution and effluent obtained from industrial area. Results were obtained at every half hour interval upto 180 min. Samples were observed for light absorption using UV-Visible spectrophotometer. Statistically, both systems reclaimed Cr and Cd from polluted water. In vitro cultures showed 67% and 93% removal of Cr and Cd from industrial wastewater whereas soil-grown plants showed 64% and 83% Cr and Cd removal. However, reduction rate was significantly higher for in vitro culture as compared to soil-grown plants. Besides other advantages, in vitro plant cultures proved to be more potent to detoxify pollutants in less time. This approach can be used for the removal of heavy metals at large scale.

Identification of chebulinic acid as potent natural inhibitor of M. tuberculosis DNA gyrase and molecular insights into its binding mode of action.

Drug resistant tuberculosis has threatened all the advances that have been made in TB control at the global stage in the last few decades. DNA gyrase enzymes are an excellent target for antibacterial drug discovery as they are involved in essential functions like DNA replication. Here we report, a successful application of high throughput virtual screening (HTVS) to identify an inhibitor of Mycobacterium DNA gyrase targeting the wild type and the most prevalent three double mutants of quinolone resistant DNA gyrase namely A90V+D94G, A74S+D94G and A90V+S91P. HTVS of 179.299 compounds gave five compounds with significant binding affinity. Extra presicion (XP) docking and MD simulations gave a clear view of their interaction pattern. Among them, chebulinic acid (CA), a phytocompound obtained from Terminalia chebula was the most potent inhibitor with significantly high XP docking score, -14.63, -16.46, -15.94 and -15.11 against wild type and three variants respectively. Simulation studies for a period of 16 ns indicated stable DNA gyrA-CA complex formation. This stable binding would result in inhibition of the enzyme by two mechanisms. Firstly, binding of CA causes displacement of catalytic Tyr129 away from its target DNA-phosphate molecule from 1.6 Å to 3.8-7.3 Å and secondly, by causing steric hindrance to the binding of DNA strand at DNA binding site of enzyme. The combined effect would result in loss of cleavage and religation activity of enzyme leading to bactericidal effect on tuberculosis. This phytocompound displays desirable quality for carrying forward as a lead compound for anti-tuberculosis drug development. The results presented here are solely based on computations and need to be validated experimentally in order to assert the proposed mechanism of action.