[Urinary N-acetyl-beta-D-glucosaminidase (NAG) excretion in women with pregnancy complicated with hypertension]. / Aktywnosc N-acetylo-beta-D-glukozaminidazy (NAG) w moczu u kobiet w ciazy powiklanej nadcisnieniem tetniczym.

OBJECTIVES: Hypertensive disorders in pregnancy are one of the major mortality risk factors for mother and fetus. Although pathomechanisms of hypertension are extreme complex, the involvement of kidneys usually occurs. N-acetyl-beta-D-glucosaminidase (NAG) is a lysosomal enzyme which is located in renal tubular cells. Therefore an elevation of urinary NAG activity serves as a marker of tubular cell damage. AIM: Evaluation of renal tubular damage in pregnant women with different types of hypertension by determination of urinary NAG activity. MATERIAL AND METHODS: The study comprised 84 pregnant women in third trimester, divided according to type of hypertension into 3 subgroups: pregnancy induced hypertension (n = 58), preeclampsia (n = 13) and chronic hypertension (n = 13). The control group comprised 36 healthy pregnant women. Urinary NAG activity was measured in the second morning urine samples by colorimetric method and the results were expressed as NAG/creatinine ratios (NAG/Cr). RESULTS: The highest NAG/Cr ratios were found in women with preeclampsia (median-1.520 U/mmol) and in women with pregnancy induced hypertension (median-0.874 U/mmol) and both results differed significantly from those in controls (median-0.782 U/mmol). There was slight positive correlation between NAG/Cr ratios and systolic blood pressure (r = 0.225, p < 0.05). CONCLUSIONS: Hypertension in pregnancy may lead to renal tubular damage, however clinical significance of this phenomenon requires further studies.

Electrophoresis patterns of proteinuria in various renal diseases of childhood.

The purpose of the study was to assess electrophoresis patterns of proteinuria in children with different renal diseases. The study comprised 52 children (30 boys and 22 girls) aged 2-18 years hospitalized in the Department of Nephrology, University Children's Hospital, Lublin. Nephrotic syndrome, glomerulonephritis and isolated proteinuria were diagnosed in 26, 11 and 15 children, respectively. Electrophoresis of urinary proteins was performed using the system SEBIA-Hydragel Proteinuria. Steroid-responsive, steroid-resistant and steroid-dependent nephrotic syndromes were characterized by glomerular selective proteinuria in 76%, 40% and 12.5% of children, respectively. In other nephrotic children glomerular nonselective proteinuria was identified. Selective glomerular proteinuria was found in 45.5% of children with glomerulonephritis. In this group glomerular nonselective proteinuria and mixed proteinuria were diagnosed in 36.4% and 18.1% of children, respectively. In the majority of children with isolated proteinuria (66,7%) nonselective pattern was revealed. In this group glomerular selective proteinuria and mixed proteinuria were observed in 13.3% and 20% of children, respectively.