In situ three-dimensional spider web construction and mechanics.

Spiders are nature's engineers that build lightweight and high-performance web architectures often several times their size and with very few supports; however, little is known about web mechanics and geometries throughout construction, especially for three-dimensional (3D) spider webs. In this work, we investigate the structure and mechanics for a Tidarren sisyphoides spider web at varying stages of construction. This is accomplished by imaging, modeling, and simulations throughout the web-building process to capture changes in the natural web geometry and the mechanical properties. We show that the foundation of the web geometry, strength, and functionality is created during the first 2 d of construction, after which the spider reinforces the existing network with limited expansion of the structure within the frame. A better understanding of the biological and mechanical performance of the 3D spider web under construction could inspire sustainable robust and resilient fiber networks, complex materials, structures, scaffolding, and self-assembly strategies for hierarchical structures and inspire additive manufacturing methods such as 3D printing as well as inspire artistic and architectural and engineering applications.

Imaging and analysis of a three-dimensional spider web architecture.

Spiders are abundantly found in nature and most ecosystems, making up more than 47 000 species. This ecological success is in part due to the exceptional mechanics of the spider web, with its strength, toughness, elasticity and robustness, which originate from its hierarchical structures all the way from sequence design to web architecture. It is a unique example in nature of high-performance material design. In particular, to survive in different environments, spiders have optimized and adapted their web architecture by providing housing, protection, and an efficient tool for catching prey. The most studied web in literature is the two-dimensional (2D) orb web, which is composed of radial and spiral threads. However, only 10% of spider species are orb-web weavers, and three-dimensional (3D) webs, such as funnel, sheet or cobwebs, are much more abundant in nature. The complex spatial network and microscale size of silk fibres are significant challenges towards determining the topology of 3D webs, and only a limited number of previous studies have attempted to quantify their structure and properties. Here, we focus on developing an innovative experimental method to directly capture the complete digital 3D spider web architecture with micron scale resolution. We built an automatic segmentation and scanning platform to obtain high-resolution 2D images of individual cross-sections of the web that were illuminated by a sheet laser. We then developed image processing algorithms to reconstruct the digital 3D fibrous network by analysing the 2D images. This digital network provides a model that contains all of the structural and topological features of the porous regions of a 3D web with high fidelity, and when combined with a mechanical model of silk materials, will allow us to directly simulate and predict the mechanical response of a realistic 3D web under mechanical loads. Our work provides a practical tool to capture the architecture of sophisticated 3D webs, and could lead to studies of the relation between architecture, material and biological functions for numerous 3D spider web applications.