Analysis of micro-relief of biofilm of yeast Candida albicans of basic plastics by the method of laser modulation interference microscopy.

The development of mycotic colonization of the base surface with further biodegradation of acrylic plastics is currently of undoubted interest. The oral cavity is a favorable ecological niche for colonization by fungi and their subsequent possible invasion into the epithelium of the oral mucosa. The method of modulation interference laser microscopy is of considerable interest to researchers in medicine in the context of obtaining the necessary information about the morphological characteristics of microbial cells and the microbiome community as a whole during the colonization of a certain ecological niche in the human body. Purpose of the study: to analyze the microrelief of the biofilm of yeast-like fungi of the species Candida albicans of base plastics of the hot type of polymerization using the method of laser modulation interference microscopy. An experimental study was carried out in order to study biofilms of yeast-like fungi of the genus Candida on samples of basic plastics, an image of a biofilm of yeast-like fungi of the species Candida albicans was obtained on the surface of a plastic of a hot type of polymerization (polymethyl methacrylate) in the visualization of the phase portrait, a description of its horizontal and vertical bioprofile. As a result of the research, the heterogeneous structure of the biofilm was determined, due to the different density and accumulation of cells along the surface, the characteristics of the surface were established in accordance with the roughness criteria. The microrelief parameters on a separately arbitrarily selected section line allow one to determine the characteristics of the biofilm in the required area and make it possible to judge the nature of its formation in a certain biological niche.

[The impact of ureteral stent surface on encrustation and biofilm formation].

INTRODUCTION: Encrustation and biofilm formation is a clinical problem occurring with indwelling urinary drainage devices routinely used in urological practice. AIM: To investigate the impact of ureteral stent surface on encrustation and biofilm formation. MATERIALS AND METHODS: Polyurethane stents of two manufacturers were examined using the scanning electron microscopy and scanning probe microscopy before use and three weeks after their initial ureteral placement in patients with no evidence of a urinary tract infection and urolithiasis. RESULTS: In one case, there were irregularities in the form of pyramids with a height of 0.15+/-0.02 m with a tendency to occur at regular intervals, forming ordered rows, with a density of 2.67 per 10 m2. The cross-sectional area of the irregularities in the probe displacement direction was 1.015 m, after use - 1.271 m (25.2% increase). In another case, pyramids with a height of 0.39+/-0.03 m (p<0.0001) were detected without the pattern of repetition in the form of a network, the density was 3.31+/-10 m2. The cross-sectional area of the irregularities was 1.158 and 2.29 m2, respectively (an increase of 97.8%). In the first case, after three weeks of stent placement, the pyramids increased twofold, were amenable to counting, the biofilms had the appearance of scattered "loose" formations. In the second case, there were polygonal conglomerates of salts dozens of times larger than the primary elements; biofilms had the appearance of well-formed massive layers. CONCLUSION: The nature of the ureteral stent surface exerts a direct independent effect on the degree of encrustation and the formation of biofilms.