RESUMO
We introduce and summarize reviews and research papers by speakers at a discussion meeting on 'Long-term potentiation: 50 years on' held at the Royal Society, London, on 20-21 November 2023. The meeting followed earlier discussion meetings marking the 30th and 40th anniversaries of the discovery of long-term potentiation. These new contributions give an overview of current research and controversies in a vibrant branch of neuroscience with important implications for our understanding of the neurobiological basis of many forms of learning and memory and a wide spectrum of neurological and cognitive disorders.This article is part of a discussion meeting issue 'Long-term potentiation: 50 years on'.
Assuntos
Potenciação de Longa Duração , Potenciação de Longa Duração/fisiologia , Humanos , Animais , História do Século XX , Aprendizagem , Memória/fisiologia , História do Século XXIRESUMO
Magnetic particle imaging is an emerging tomographic technique with the potential for simultaneous high-resolution, high-sensitivity, and real-time imaging. Magnetic particle imaging is based on the unique behavior of superparamagnetic iron oxide nanoparticles modeled by the Langevin theory, with the ability to track and quantify nanoparticle concentrations without tissue background noise. It is a promising new imaging technique for multiple applications, including vascular and perfusion imaging, oncology imaging, cell tracking, inflammation imaging, and trauma imaging. In particular, many neuroimaging applications may be enabled and enhanced with magnetic particle imaging. In this review, we will provide an overview of magnetic particle imaging principles and implementation, current applications, promising neuroimaging applications, and practical considerations.
Assuntos
Fenômenos Magnéticos , Neuroimagem/métodos , Humanos , Processamento de Imagem Assistida por Computador/métodos , NanopartículasRESUMO
We summarize the reviews and research papers submitted by speakers at a discussion meeting on Synaptic Plasticity in Health and Disease held at the Royal Society, London on 2-3 December 2013, and a subsequent satellite meeting convened at the Royal Society/Kavli Centre at Chicheley Hall on 4-5 December 2013. Together, these contributions give an overview of current research and controversies in a vibrant branch of neuroscience with important implications for the understanding of many forms of learning and memory, and a wide spectrum of neurological and cognitive disorders.
Assuntos
Transtornos Mentais/fisiopatologia , Doenças do Sistema Nervoso/fisiopatologia , Plasticidade Neuronal/fisiologia , Pesquisa/tendências , Sinapses/fisiologia , Cognição/fisiologia , Humanos , Aprendizagem/fisiologia , Memória/fisiologiaRESUMO
AIMS: To elucidate the mechanism of action of a nonchemical microbial control technology employing coupled hydrodynamic and ultrasound-induced stress. METHODS & RESULTS: The effects of a laboratory model system using a commercial nonchemical device on Pseudomonas putida revealed growth and respiration were inhibited without a loss of viability from the treated population. Damage to cell membranes was evident using fluorescent microscopy and a reporter strain containing lux genes fused with a membrane damage stress-response promoter. Other reporter strains also indicated the possible involvement of DNA and protein repair systems. A consequence of treatment was a reduced ability to form biofilms. CONCLUSIONS: The nonchemical device caused a biostatic effect on treated cells induced by sublethal damage to several cellular systems, including cell membranes. SIGNIFICANCE AND IMPACT OF THE STUDY: The study demonstrates that biostasis can be an effective mechanism for microbial control in some industrial systems and provides insight into understanding and applying this device and other nonchemical microbial control technologies to real-world problems of microbial contamination.
Assuntos
Membrana Celular/patologia , Pseudomonas putida/crescimento & desenvolvimento , Som , Ultrassom , Biofilmes/crescimento & desenvolvimento , Contagem de Colônia Microbiana , Escherichia coli/genética , Escherichia coli/crescimento & desenvolvimento , Escherichia coli/efeitos da radiação , Escherichia coli/ultraestrutura , Genes Reporter , Hidrodinâmica , Regiões Promotoras Genéticas , Pseudomonas putida/genética , Pseudomonas putida/efeitos da radiação , Pseudomonas putida/ultraestrutura , Estresse FisiológicoRESUMO
Synaptic efficacy following long-term potentiation (LTP) and memory consolidation is associated with changes in the expression of immediate early genes (IEGs). These changes are often accompanied by increased expression of glial fibrillary acidic protein (GFAP). While the protein products of the majority of IEGs are mainly restricted to the cell body, Arg3.1/Arc product is rapidly delivered to dendrites, where it accumulates close to synaptic sites. Arg3.1/Arc protein was originally considered neurone specific; however, we have recently found Arg3.1/Arc immunoreactivity (Arg3.1/Arc-IR) within glial cells and demonstrated its increased expression after LTP in the hippocampal dentate gyrus (DG). Here, we have further investigated this novel finding, using electron microscopic immunocytochemistry to determine the localization and sub-cellular distribution of Arg3.1/Arc protein in GFAP positive glia (GFAP-IR) in the DG. Arg3.1/Arc labelling was seen prominently in GFAP-IR glial cell bodies and in large- and medium-sized glial filamentous processes. GFAP-labelled medium-small peri-synaptic glial profiles also displayed Arg3.1/Arc-IR; however, the very thin and distal glial filaments only displayed Arc-IR. Arc-IR was distributed throughout the cytoplasm, often associated with GFAP filaments, and along the plasma membrane of glial processes. Peri-synaptic glial Arg3.1/Arc-IR processes were apposed to pre- and/or post-synaptic profiles at asymmetric axospinous synapses. These data, taken with our earlier study which provided evidence for an increase in astrocytic Arg3.1/Arc-IR after the induction of LTP, suggest a role for glial Arg3.1/Arc in structural and synaptic plasticity which may be critical for the maintenance of cognitive functions.
Assuntos
Astrócitos/metabolismo , Proteínas do Citoesqueleto/imunologia , Giro Denteado/metabolismo , Proteína Glial Fibrilar Ácida/metabolismo , Hipocampo/metabolismo , Proteínas do Tecido Nervoso/imunologia , Animais , Astrócitos/ultraestrutura , Proteínas do Citoesqueleto/genética , Proteínas do Citoesqueleto/metabolismo , Proteínas do Citoesqueleto/ultraestrutura , Giro Denteado/ultraestrutura , Hipocampo/ultraestrutura , Masculino , Camundongos , Camundongos Knockout , Proteínas do Tecido Nervoso/genética , Proteínas do Tecido Nervoso/metabolismo , Proteínas do Tecido Nervoso/ultraestrutura , Ratos , Ratos Sprague-Dawley , Proteínas Recombinantes/imunologia , Proteínas Recombinantes/metabolismo , Proteínas Recombinantes/ultraestruturaRESUMO
A second-generation 4,959 element cDNA microarray has been created and evaluated for its potential use in examining the avian innate immune response. The elements in this array were obtained from EST libraries of stimulated avian PMNC-derived monocytes/macrophages and supplemented by genes of interest from several specific innate immune pathways. The elements are spotted in triplicate resulting in 14,877 total spots per slide. The avian innate immunity microarray (AIIM) contains 25 avian interleukin, chemokine, and cytokine elements. The array also contains elements for several innate immune pathways, including genes involved in the Toll-like receptor (TLR) pathway (including six of the currently known avian TLR receptors), avian interferon/antiviral response pathway genes, and genes involved in apoptosis, antigen presentation and the oxidative burst. The AIIM can be used to evaluate global gene expression patterns in a number of immunologically relevant tissues and in chickens, turkeys and ducks. The array has also been evaluated for its ability to monitor the avian immune response to both bacterial (avian pathogenic Escherichia coli) and viral (avian influenza) avian pathogens.
Assuntos
Aves/genética , Aves/imunologia , Genômica , Imunidade Inata/genética , Imunidade Inata/imunologia , Animais , Aves/microbiologia , Aves/virologia , Células Cultivadas , Citocinas/genética , Citocinas/imunologia , Infecções por Escherichia coli/genética , Infecções por Escherichia coli/imunologia , Infecções por Escherichia coli/microbiologia , Regulação da Expressão Gênica , Vírus da Influenza A/fisiologia , Influenza Aviária/genética , Influenza Aviária/imunologia , Influenza Aviária/virologia , Análise de Sequência com Séries de Oligonucleotídeos , Transdução de Sinais , Receptores Toll-Like/genéticaRESUMO
Long-term potentiation (LTP) is a well-characterized form of synaptic plasticity that fulfils many of the criteria for a neural correlate of memory. LTP has been studied in a variety of animal models and, in rodents in particular, there is now a strong body of evidence demonstrating common underlying molecular mechanisms in LTP and memory. Results are beginning to emerge from studies of neural plasticity in humans. This review will summarize findings demonstrating that synaptic LTP can be induced in human CNS tissue and that rodent and human LTP probably share similar molecular mechanisms. We will also discuss the application of non-invasive stimulation techniques to awake human subjects to induce LTP-like long-lasting changes in localized neural activity. These techniques have potential therapeutic application in manipulating neural plasticity to treat a variety of conditions, including depression, Parkinson's disease, epilepsy and neuropathic pain.
Assuntos
Encéfalo/fisiologia , Potenciação de Longa Duração/fisiologia , Animais , Encefalopatias/terapia , Transtorno Depressivo/terapia , Humanos , Hiperalgesia/terapia , Memória/fisiologia , Camundongos , Plasticidade Neuronal/fisiologia , Estimulação Física/métodos , Ratos , Receptores de N-Metil-D-Aspartato/fisiologia , Estimulação Magnética TranscranianaRESUMO
Cell transplantation offers a potential new treatment for stroke. Animal studies using models that produce ischemic damage in both the striatum and the frontal cortex have shown beneficial effects when hNT cells (postmitotic immature neurons) were transplanted into the ischemic striatum. In this study, we investigated the effect of hNT cells in a model of stroke in which the striatum remains intact and damage is restricted to the cortex. hNT cells were transplanted into the ischemic cortex 1 week after stroke induced by distal middle cerebral artery occlusion (dMCAo). The cells exhibited robust survival at 4 weeks posttransplant even at the lesion border. hNT cells did not migrate, but they did extend long neurites into the surrounding parenchyma mainly through the white matter. Neurite extension was predominantly toward the lesion in ischemic animals but was bidirectional in uninjured animals. Extension of neurites through the cortex toward the lesion was also seen when there was some surviving cortical tissue between the graft and the infarct. Prolonged deficits were obtained in four tests of sensory-motor function. hNT-transplanted animals showed a significant improvement in functional recovery on one motor test, but there was no effect on the other three tests relative to control animals. Thus, despite clear evidence of graft survival and neurite extension, the functional benefit of hNT cells after ischemia is not guaranteed. Functional benefit could depend on other variables, such as infarct location, whether the cells mature, the behavioral tests employed, rehabilitation training, or as yet unidentified factors.
Assuntos
Isquemia Encefálica/fisiopatologia , Isquemia Encefálica/cirurgia , Transplante de Células/métodos , Neurônios/fisiologia , Recuperação de Função Fisiológica/fisiologia , Células-Tronco/fisiologia , Análise de Variância , Animais , Comportamento Animal/fisiologia , Isquemia Encefálica/metabolismo , Isquemia Encefálica/patologia , Linhagem Celular , Sobrevivência Celular/fisiologia , Modelos Animais de Doenças , Humanos , Imuno-Histoquímica/métodos , Masculino , Atividade Motora/fisiologia , Postura/fisiologia , Desempenho Psicomotor/fisiologia , Ratos , Ratos Sprague-Dawley , Fatores de TempoRESUMO
p25, a degradation product of p35, has been reported to accumulate in the forebrain of patients with Alzheimer's disease. p25 as well as p35 are activators of cyclin-dependent kinase 5 (Cdk5) although p25/Cdk5 and p35/Cdk5 complexes have distinct properties. Several mouse models with high levels of p25 expression exhibit signs of neurodegeneration. On the contrary, we have shown that low levels of p25 expression do not cause neurodegeneration and are even beneficial for particular types of learning and memory [Angelo et al., (2003) Eur J. Neurosci., 18, 423-431]. Here, we have studied the influence of low-level p25 expression in hippocampal synaptic plasticity and in learning and memory for each sex separately in two different genetic backgrounds (129B6F1 and C57BL/6). Surprisingly, we found that low-level p25 expression had different consequences in male and female mutants. In the two genetic backgrounds LTP induced by a strong stimulation of the Schaffer's collaterals (four trains, 1-s duration, 5-min interval) was severely impaired in male, but not in female, p25 mutants. Furthermore, in the two genetic backgrounds spatial learning in the Morris water maze was faster in female p25 mutants than in male transgenic mice. These results suggest that, in women, the production of p25 in Alzheimer's disease could be a compensation for some early learning and memory deficits.
Assuntos
Predisposição Genética para Doença/genética , Deficiências da Aprendizagem/genética , Transtornos da Memória/genética , Proteínas do Tecido Nervoso/genética , Plasticidade Neuronal/genética , Caracteres Sexuais , Animais , Quinase 5 Dependente de Ciclina , Quinases Ciclina-Dependentes/metabolismo , Modelos Animais de Doenças , Feminino , Regulação da Expressão Gênica no Desenvolvimento/genética , Hipocampo/crescimento & desenvolvimento , Hipocampo/metabolismo , Hipocampo/fisiopatologia , Potenciação de Longa Duração/genética , Masculino , Aprendizagem em Labirinto/fisiologia , Transtornos da Memória/metabolismo , Transtornos da Memória/fisiopatologia , Camundongos , Camundongos Endogâmicos C57BL , Camundongos TransgênicosRESUMO
Electron microscopic immunocytochemical methods were used to determine the localization, subcellular distribution and expression of activity-regulated cytoskeletal protein (Arc/Arg3.1) in dentate gyrus after unilateral induction of long-term potentiation (LTP) in the perforant pathway of anaesthetized rats. At 2 h post-induction, immunoreaction product was visible in the dentate gyrus in both the granule cell and molecular layers. Arc expression was higher in the potentiated than the unstimulated contralateral hemisphere. Single-section electron microscopy analysis in unstimulated tissue and in tissue prepared 2 and 4 h after LTP induction showed Arc immunoreactivity (Arc-IR) in dendrites, dendritic spines and glia. Arc-IR was associated with synaptic and non-synaptic plasma membrane apposed to axon terminals and with cytoplasmic organelles, including the cytoskeleton. Arc-IR was also present in neuronal perikarya and there was occasional labelling of nuclei and axons. At 2 h post-LTP induction, there were significant increases in Arc-IR within the granule cell and molecular layers of the dentate gyrus and particularly within the middle molecular layer relative to the inner and outer molecular layers. This increase in Arc expression 2 h after LTP induction was blocked by the N-methyl-D-aspartate receptor antagonist (RS)-3-2-carboxypiperazin-4-yl-propyl-1-phosphonic acid. In animals killed 4 h after LTP induction, Arc expression had declined and differences between the potentiated and unpotentiated hemispheres were no longer significant. Our data provide ultrastructural evidence for a transient LTP-associated increase in the expression of Arc protein in the middle molecular layer of the dentate gyrus, with preferential targeting to dendrites, dendritic spines and glia.
Assuntos
Espinhas Dendríticas/metabolismo , Giro Denteado/fisiologia , Proteínas Imediatamente Precoces/metabolismo , Potenciação de Longa Duração/fisiologia , Proteínas do Tecido Nervoso/metabolismo , Neuroglia/metabolismo , Animais , Axônios/metabolismo , Axônios/ultraestrutura , Proteínas do Citoesqueleto , Dendritos/metabolismo , Dendritos/ultraestrutura , Espinhas Dendríticas/ultraestrutura , Antagonistas de Aminoácidos Excitatórios/farmacologia , Potenciação de Longa Duração/efeitos dos fármacos , Masculino , Microscopia Eletrônica , Neuroglia/ultraestrutura , Plasticidade Neuronal/efeitos dos fármacos , Plasticidade Neuronal/fisiologia , Piperazinas/farmacologia , Ratos , Ratos Sprague-DawleyRESUMO
A major role has been postulated for a maintained increase in the autonomous activity of CaMKII in the expression of long-term potentiation (LTP). However, attempts to inhibit the expression of LTP with CaMKII inhibitors have yielded inconsistent results. Here we compare the changes in CaMKII autonomous activity and phosphorylation at Thr286 of alphaCaMKII in rat hippocampal slices using chemical or tetanic stimulation to produce either LTP or short-term potentiation (STP). Tetanus-induced LTP in area CA1 requires CaMKII activation and Thr286 phosphorylation of alphaCaMKII, but we did not observe an increase in autonomous activity. Next we induced LTP by 10 min exposure to 25 mM tetraethyl-ammonium (TEA) or 5 min exposure to 41 mM potassium (K) after pretreatment with calyculin A. Exposure to K alone produced STP. These protocols allowed us to monitor temporal changes in autonomous activity during and after exposure to the potentiating chemical stimulus. In chemically induced LTP, autonomous activity was maximally increased within 30 s whereas this increase was significantly delayed in STP. However, in both LTP and STP the two-fold increase in autonomous activity measured immediately after stimulation was short-lived, returning to baseline within 2-5 min after re-exposure to normal ACSF. In LTP, but not in STP, the phosphorylation of alphaCaMKII at Thr286 persisted for at least 60 min after stimulation. These results confirm that LTP is associated with a maintained increase in autophosphorylation at Thr286 but indicate that a persistent increase in the autonomous activity of CaMKII is not required for the expression of LTP.
Assuntos
Proteínas Quinases Dependentes de Cálcio-Calmodulina/metabolismo , Ativação Enzimática , Hipocampo/fisiologia , Potenciação de Longa Duração/fisiologia , Animais , Western Blotting/métodos , Proteína Quinase Tipo 2 Dependente de Cálcio-Calmodulina , Estimulação Elétrica , Potenciais Pós-Sinápticos Excitadores/efeitos dos fármacos , Potenciais Pós-Sinápticos Excitadores/efeitos da radiação , Hipocampo/anatomia & histologia , Hipocampo/efeitos dos fármacos , Hipocampo/efeitos da radiação , Técnicas In Vitro , Potenciação de Longa Duração/efeitos dos fármacos , Potenciação de Longa Duração/efeitos da radiação , Masculino , Fosforilação/efeitos dos fármacos , Fosforilação/efeitos da radiação , Potássio/farmacologia , Bloqueadores dos Canais de Potássio/farmacologia , Ratos , Ratos Sprague-Dawley , Tetraetilamônio/farmacologia , Treonina/metabolismo , Fatores de TempoRESUMO
In anaesthetised rats, long-term potentiation (LTP) was induced unilaterally in the dentate gyrus by tetanic stimulation of the perforant path. Animals were killed 6 h after LTP induction and dendritic spines and synapses in tetanised and untetanised (contralateral) hippocampal tissue from the middle molecular layer (MML) were examined in the electron microscope using stereological analysis. Three-dimensional reconstructions were also used for the first time in LTP studies in vivo, with up to 130 ultrathin serial sections analysed per MML dendritic segment. A volume sampling procedure revealed no significant changes in hippocampal volume after LTP and an unbiased counting method demonstrated no significant changes in synapse density in potentiated compared with control tissue. In the potentiated hemisphere, there were changes in the proportion of different spine types and their synaptic contacts. We found an increase in the percentage of synapses on thin dendritic spines, a decrease in synapses on both stubby spines and dendritic shafts, but no change in the proportion of synapses on mushroom spines. Analysis of three-dimensional reconstructions of thin and mushroom spines following LTP induction revealed a significant increase in their volume and area. We also found an increase in volume and area of unperforated (macular) and perforated (segmented) postsynaptic densities. Our data demonstrate that whilst there is no change in synapse density 6 h after the induction of LTP in vivo, there is a considerable restructuring of pre-existing synapses, with shaft and stubby spines transforming to thin dendritic spines, and mushroom spines changing only in shape and volume.
Assuntos
Giro Denteado/fisiologia , Giro Denteado/ultraestrutura , Potenciação de Longa Duração/fisiologia , Plasticidade Neuronal , Sinapses/fisiologia , Sinapses/ultraestrutura , Animais , Espinhas Dendríticas/ultraestrutura , Processamento de Imagem Assistida por Computador , Imageamento Tridimensional , Masculino , Microscopia Eletrônica , Ratos , Ratos Sprague-DawleyRESUMO
We have isolated a novel transcript with homology to the major microtubule-associated protein in dividing sea urchin embryos, EMAP. The protein has a predicted MW of approximately 180 kDa and we have named it Eml5 (EMAP-like protein 5). Eml5 contains 11 putative WD40 domains and 3 hydrophobic stretches of 43 aa, HELP domains, which have been suggested to be involved in microtubule binding. Eml5 appears to consist of two tandem repeats of the complete EMAP protein separated by a putative dimerization domain. Eml5 mRNA and protein is expressed at high levels in the hippocampus, cerebellum and olfactory bulb, as determined by in situ hybridization and immunocytochemistry. Eml5 transcripts can be detected in fore- and hindbrain structures from embryonic day 13 onwards. Because other EMAP-like proteins are involved in regulating microtubule dynamics, it is likely that Eml5 plays a role in the regulation of cytoskeletal rearrangements during neuronal development and in adult brain
Assuntos
Encéfalo/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Proteínas Associadas aos Microtúbulos/genética , Proteínas do Tecido Nervoso/genética , Processamento Alternativo , Sequência de Aminoácidos , Animais , Sítios de Ligação/genética , Northern Blotting , Encéfalo/embriologia , Encéfalo/crescimento & desenvolvimento , Células COS , Chlorocebus aethiops , Clonagem Molecular , DNA Complementar/química , DNA Complementar/genética , Proteínas de Fluorescência Verde , Imuno-Histoquímica , Hibridização In Situ , Proteínas Luminescentes/genética , Proteínas Luminescentes/metabolismo , Proteínas Associadas aos Microtúbulos/metabolismo , Dados de Sequência Molecular , Proteínas do Tecido Nervoso/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Ratos , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismo , Alinhamento de Sequência , Análise de Sequência de DNA , Homologia de Sequência de AminoácidosRESUMO
We characterize the survival, migration, and differentiation of human neurospheres derived from CNS stem cells transplanted into the ischemic cortex of rats 7 days after distal middle cerebral artery occlusion. Transplanted neurospheres survived robustly in naive and ischemic brains 4 wk posttransplant. Survival was influenced by proximity of the graft to the stroke lesion and was negatively correlated with the number of IB4-positive inflammatory cells. Targeted migration of the human cells was seen in ischemic animals, with many human cells migrating long distances ( approximately 1.2 mm) predominantly toward the lesion; in naive rats, cells migrated radially from the injection site in smaller number and over shorter distances (0.2 mm). The majority of migrating cells in ischemic rats had a neuronal phenotype. Migrating cells between the graft and the lesion expressed the neuroblast marker doublecortin, whereas human cells at the lesion border expressed the immature neuronal marker beta-tubulin, although a small percentage of cells at the lesion border also expressed glial fibrillary acid protein (GFAP). Thus, transplanted human CNS (hCNS)-derived neurospheres survived robustly in naive and ischemic brains, and the microenvironment influenced their migration and fate.
Assuntos
Isquemia Encefálica/terapia , Movimento Celular , Neurônios/citologia , Transplante de Células-Tronco , Animais , Arteriopatias Oclusivas/terapia , Biomarcadores/análise , Isquemia Encefálica/patologia , Diferenciação Celular , Sobrevivência Celular , Córtex Cerebral/patologia , Proteína Duplacortina , Feto/citologia , Humanos , Ratos , Transplante HeterólogoRESUMO
We employed differential display of expressed mRNAs (Liang, P., and Pardee, A. B. (1992) Science 257, 967-971) to identify genes up-regulated after long term potentiation (LTP) induction in the hippocampus of awake adult rats. In situ hybridization confirmed the differential expression of five independently amplified clones representing two distinct transcripts, cl13/19/90 and cl95/96. Neither cl13/19/90 nor cl95/96 showed significant sequence homology to known transcripts (mRNA or expressed sequence tag) or to the mouse or human genome. However, comparison with the rat genome revealed that they are localized to a predicted intron of the phosphodiesterase Pde10A gene. cl13/19/90 and cl95/96 are likely to be part of the Pde10A primary transcript as, using reverse transcriptase-PCR, we could specifically amplify distinct introns of the Pde10A primary transcript, and in situ hybridization demonstrated that a subset of Pde10A splice variants are also up-regulated after LTP induction. These results indicate that amplification of a primary transcript can faithfully report gene activity and that differential display can be used to identify differential expression of RNA species other than mRNA. In transiently transfected Cos7 cells, Pde10A3 reduces the atrial natriuretic peptide-induced elevation in cGMP levels without affecting basal cGMP levels. This cellular function of LTP-associated Pde10A transcripts argues for a role of the cGMP/cGMP-dependent kinase pathway in long term synaptic plasticity.
Assuntos
Potenciação de Longa Duração/genética , Diester Fosfórico Hidrolases/genética , Animais , Humanos , Íntrons , Isoenzimas/genética , Masculino , Camundongos , Dados de Sequência Molecular , Plasticidade Neuronal/genética , Splicing de RNA , Ratos , Ratos Sprague-Dawley , Regulação para Cima/genéticaRESUMO
The late temporal component of long-term potentiation (LTP), a putative neural mechanism for information storage in the brain, is protein synthesis-dependent, but the site of obligatory protein synthesis is not known. Here we show that when the protein synthesis inhibitor emetine is applied locally to the apical dendritic field of CA1 pyramidal cells in the murine hippocampus, late LTP is impaired at apical but not at basal dendrites, and conversely when emetine is applied locally to basal dendrites, late LTP is impaired only at basal dendrites. Thus, local protein synthesis modulates the expression of tetanically induced late LTP at Schaffer-commissural synapses on CA1 pyramidal cells.
Assuntos
Dendritos/fisiologia , Hipocampo/fisiologia , Potenciação de Longa Duração/fisiologia , Biossíntese de Proteínas , Animais , Isótopos de Carbono/metabolismo , Dendritos/metabolismo , Estimulação Elétrica , Eletrofisiologia , Emetina/farmacologia , Hipocampo/citologia , Técnicas In Vitro , Masculino , Camundongos , Inibidores da Síntese de Proteínas , Fatores de Tempo , Valina/metabolismoRESUMO
We have examined the effect of arachidonic acid on the transient increases in intracellular Ca2+ evoked by NMDA and AMPA in cultured hippocampal pyramidal cells loaded with Fura-2 AM. Repeated brief pulses of NMDA elicited Ca2+ transients that showed a modest run down. This run down was enhanced if the preparation was shielded from UV light and was reduced by conducting the experiments in the presence of the nitric oxide synthase inhibitor l-nitroarginine (100 micro m). Arachidonic acid (2 micro m) enhanced the Ca2+ transients evoked by NMDA but not those evoked by AMPA. Other C20 unsaturated fatty acids did not alter the time course of the response to NMDA. These experiments suggest that elevated intracellular Ca2+ activates nitric oxide synthase and the resulting synthesis of nitric oxide depresses the Ca2+ response to NMDA while arachidonic acid augments these responses. Therefore two substances implicated in synaptic plasticity (arachidonic acid and nitric oxide) differentially modulate NMDA-mediated Ca2+ entry into hippocampal neurons.
Assuntos
Ácido Araquidônico/farmacologia , Cálcio/metabolismo , Agonistas de Aminoácidos Excitatórios/farmacologia , Fura-2/análogos & derivados , Hipocampo/citologia , N-Metilaspartato/farmacologia , Óxido Nítrico/farmacologia , Células Piramidais/efeitos dos fármacos , Animais , Animais Recém-Nascidos , Cádmio/farmacologia , Células Cultivadas , Interações Medicamentosas , Ácidos Eicosanoicos/farmacologia , Ácido Eicosapentaenoico , Ácidos Graxos Insaturados/farmacologia , Sequestradores de Radicais Livres/farmacologia , Fura-2/metabolismo , Células Piramidais/metabolismo , Ratos , Fatores de Tempo , Ácido alfa-Amino-3-hidroxi-5-metil-4-isoxazol Propiônico/farmacologiaRESUMO
In the mid-1960s, it was generally agreed that the engram, the neural trace of previously experienced events, must be encoded by Hebb-like neurons in which synaptic efficacy could be modified by activity. Here, I describe my attempts as a PhD student at McGill University, Montreal, to find rules governing cortical plasticity in the neocortex, and having failed, why the hippocampus seemed to offer a far better prospect.
Assuntos
Plasticidade Neuronal/fisiologia , Animais , Canadá , Hipocampo/fisiologia , História do Século XX , Potenciação de Longa Duração/fisiologia , Memória/fisiologia , Neocórtex/fisiologia , Neurofisiologia/históriaRESUMO
We have used a glutamate-specific dialysis electrode to obtain real-time measurements of changes in the concentration of glutamate in the extracellular space of the hippocampus during low-frequency stimulation and following the induction of long-term potentiation (LTP). In the dentate gyrus, stimulation of the perforant path at 2 Hz for 2 min produced a transient increase in glutamate current relative to the basal value at control rates of stimulation (0.033 Hz). This activity-dependent glutamate current was significantly enhanced 35 and 90 min after the induction of LTP. The maximal 2 Hz signal was obtained during post-tetanic potentiation (PTP). There was also a more gradual increase in the basal level of extracellular glutamate following the induction of LTP. Both the basal and activity-dependent increases in glutamate current induced by tetanic stimulation were blocked by local infusion of the N-methyl-D-aspartate receptor antagonist D-APV. In areas CA1 and CA3 we were unable to detect a 2 Hz glutamate signal either before or after the induction of LTP, possibly owing to a more avid uptake of glutamate in the pyramidal cell fields. These results demonstrate that LTP in the dentate gyrus is associated with a greater concentration of extracellular glutamate following activation of potentiated synapses, either because potentiated synapses release more transmitter per impulse, or because of reduced uptake by glutamate transporters. We present arguments favouring increased release rather than decreased uptake.