RESUMO
This report describes a family outbreak of verocytotoxigenic Escherichia coli O157 (VTEC) infection, involving nine persons from one extended family, which occurred in eastern Slovakia. Three children suffered from haemolytic uraemic syndrome, two children had bloody diarrhoea, and four adults were asymptomatic carriers. Fourteen sorbitol-non-fermenting E. coli O157 isolates harbouring the vtx2, eae and ehxA genes were obtained. Verocytotoxin 2 activity was demonstrated in all 14 isolates. After epidemiological surveillance, the source of infection was identified as unpasteurised cow's milk.
Assuntos
Colite/epidemiologia , Surtos de Doenças , Escherichia coli O157/isolamento & purificação , Síndrome Hemolítico-Urêmica/epidemiologia , Leite/microbiologia , Toxinas Shiga/metabolismo , Adulto , Animais , Bovinos , Criança , Pré-Escolar , Colite/microbiologia , Infecções por Escherichia coli/epidemiologia , Infecções por Escherichia coli/microbiologia , Escherichia coli O157/genética , Escherichia coli O157/metabolismo , Escherichia coli O157/patogenicidade , Saúde da Família , Feminino , Síndrome Hemolítico-Urêmica/microbiologia , Hemorragia/epidemiologia , Hemorragia/microbiologia , Humanos , Lactente , Masculino , Toxinas Shiga/genética , Eslováquia/epidemiologiaRESUMO
The polymorphisms of the tumor suppressor gene p53 in exon 4 (p53 BstUI) and in intron 6 (p53 MspI) have been suggested to be associated with the genetically determined susceptibility in diverse types of human cancer. In our hospital-based case-control study, we examined the allele and genotype incidence of these polymorphisms as well as their haplotype combinations in 60 brain tumor patients (27 males and 33 females) and 183 controls without malignancies. The genotype characteristics were determined by the PCR-based RFLP method using DNA extracted from peripheral blood. In this study we show that the p53 BstUI and the p53 MspI polymorphisms are not associated with increased risk of brain tumors. Thus, we conclude that the p53 BstUI and the p53 MspI polymorphic sites within the tumor suppressor gene p53 do not represent genetic determinants of susceptibility to brain tumors.
Assuntos
Neoplasias Encefálicas/genética , Genes p53/genética , Adulto , Idoso , Alelos , Eletroforese em Gel de Poliacrilamida , Feminino , Frequência do Gene , Genótipo , Haplótipos , Humanos , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Razão de Chances , Polimorfismo Genético/genéticaRESUMO
Fifty Escherichia coli strains isolated from stool samples of 51 healthy children, 143 strains isolated from stool samples of 327 children with diarrhea and 24 strains isolated from stool samples of 21 children with suspected hemolytic uremic syndrome were examined for the presence of Shiga toxin-producing E. coli virulence factors (shiga toxin 1 and 2, intimin and enterohemolysin) and their genes. Vero-cell assay and latex agglutination were used for detection of Shiga toxin 1 and 2, TSB agar with washed erythrocytes was used for detection of enterohemolysin; genes encoding shiga toxin 1 and 2, intimin and enterohemolysin were detected using multiplex PCR. The presence of E. coli strains harboring genes encoding shiga toxin 1 and 2 (12 strains), intimin (34 strains) and enterohemolysin (12 strains) was demonstrated.
Assuntos
Adesinas Bacterianas/análise , Proteínas de Transporte/análise , Infecções por Escherichia coli/microbiologia , Proteínas de Escherichia coli , Escherichia coli/química , Proteínas Hemolisinas/análise , Toxinas Shiga/análise , Criança , Pré-Escolar , Escherichia coli/isolamento & purificação , Fezes/microbiologia , Humanos , Reação em Cadeia da Polimerase , EslováquiaRESUMO
Occurrence of cnf1+ E. coli pathogenic strains among extraintestinal E. coli isolates was evaluated to explain an impact of cytotoxic necrotizing factor type 1 (CNF1) in human infections. A total of 120 E. coli isolates were characterized for presence of virulence factors cnf1- and pap--specific sequences by PCR, and the production of alpha-hemolysin using blood agar-plate test. Different association patterns among the detected virulence factors were obtained by comparison of various groups of clinical E. coli isolates. These differences probably reflect a potential impact of CNF1 in the colonization of vaginal environment.
Assuntos
Toxinas Bacterianas/genética , Citotoxinas/genética , Infecções por Escherichia coli/microbiologia , Proteínas de Escherichia coli/genética , Escherichia coli/patogenicidade , Proteínas Hemolisinas/genética , Adulto , Toxinas Bacterianas/metabolismo , Criança , Pré-Escolar , Citotoxinas/metabolismo , Escherichia coli/genética , Proteínas de Escherichia coli/metabolismo , Feminino , Fímbrias Bacterianas/genética , Proteínas Hemolisinas/metabolismo , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Análise de Sequência de DNA , Virulência/genéticaRESUMO
P-fimbriae, S-fimbriae and AFA-adhesins are virulence factors responsible for adherence of Escherichia coli strains to extraintestinal host-cell surface. Detection of pap-, sfa- and afa-specific sequences performed by PCR revealed 74% pap+, 65% sfa+, and 8.3% afa+ strains in a group of 84 extraintestinal E. coli isolates. Detection in a group of fecal strains showed 29% pap+, 21% sfa+ and 4% afa+ strains. pap together with sfa were found as the most frequent combination (56%) among extraintestinal isolates probably due to localization of pap- and sfa-operons on a common pathogenicity island. The occurrence of afa-specific sequence among 56 urine strains was 11%, although no afa+ strain was detected among 28 gynecological isolates. No strains with detected adhesin operons were found among twenty (24%) extraintestinal E. coli strains.
Assuntos
Adesinas de Escherichia coli/genética , Aderência Bacteriana/fisiologia , Escherichia coli/genética , Fímbrias Bacterianas/fisiologia , Adesinas de Escherichia coli/fisiologia , Adulto , Criança , Pré-Escolar , Cistite/microbiologia , DNA Bacteriano/química , DNA Bacteriano/genética , Escherichia coli/isolamento & purificação , Escherichia coli/patogenicidade , Infecções por Escherichia coli/microbiologia , Feminino , Doenças dos Genitais Femininos/microbiologia , Humanos , Lactente , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Pielonefrite/microbiologia , VirulênciaRESUMO
We tested the codon 72 single nucleotide polymorphism (SNP) of the tumor suppressor gene p53 for association with lung cancer. In our hospital-based case-control study, 168 lung cancer patients (134 males and 34 females) and 148 controls without malignant diseases were recruited. The genotype characteristics were determined by PCR-based RFLP method using DNA extracted from peripheral blood. Only in lung cancer patients but not in the controls we found both significant decrease of A1 allele of the p53 codon 72 (p=0.024, OR 0.56, 95% CI 0.43-0.72) and A1/A1 homozygous genotype (p=0.006, OR 0.27,95% CI 0.15-0.51). The results of this study suggest a protective effect of A1 allele against lung cancer.