[Glycodelin as a potential marker of female genital system tumors (a review of literature)].

The authors present the currently available data on the expression of glycodelin, a unique secretory protein of the human reproductive system, which has gender-dependent glycosylation, immunosuppressive and morphogenetic activities, in gynecological cancers. They also discuss the theoretical and practical aspects of studying glycodelin as an effector and a marker molecule of oncogenesis.

[Non-developing pregnancy: histological and immunohistochemical markers of endocrine disorders in endometrial scrapes].

Histological and immunohistological (monoclonal antibodies against desmin, vimentin, glycodelin, IGFBP-1, estrogen receptors and progesterone receptors) studies were carried out to examine uterine scrapes from 47 healthy women - medical abortions from 6 to 10 weeks of conception and from 44 patients after non-developing pregnancy in the first trimester (recurrent miscarriage), which had been induced by verified endocrine disorders. Among them, three isolated pathogenetic types were identified: 1) uterine gland retardation as preserved islets of cylindrical glands with a narrow lumen or, on the contrary, areas with enlarged glands and atrophic epitheliocytes; immunohistochemically, they exhibited a drastic reduction in the expression of glycodelin, the major local immunosuppressor; 2) incomplete decidualization when it in the presence of progesterone deficiency was delayed at the state of predecidual and decidual cells retaining the smooth muscle cell marker - desmin; 3) verified progesterone receptor insufficiency. Each of the given clinical and morphological types of endocrine causes of non-developing pregnancy requires that an individual approach be applied to examining and treating these patients.

[Expression of endometrial proteins in the uterine glands in physiological and non-developing pregnancy]. / Ekspressiia éndometrial'nykh belkov v matochnykh zhelezakh pri fiziologicheskoi i nerazvivaiushcheisia beremennosti.

It is established that at the 1st trimester of the progressing pregnancy uterine glands produce both endometrial proteins (placental al-microglobulin--PAMG) and alpha 2-fertility microglobulin (AFMB) but with different intensity: maximum level of PAMG is at early periods (4-5 weeks) but 8-9 weeks of gestation for AFMB. Production deficiency of PAMG and AFMB in the glands epithelium is found in 90% cases of non-developing pregnancy (4-7 weeks) and this is obviously one of the causes of early pregnancy interruption.

[Level of placental alpha1-microglobulin in maternal serum as a marker of intrauterine fetal pathology]. / Uroven' platsentarnogo alpha1-mikroglobulina v materinskoi syvorotke kak pokazatel' vnutriutrobnoi patologii ploda.

Serum levels of placental alpha 1-microglobulin (PAMG) were measured during the third trimester in 21 women with normal pregnancy, 24 with extragenital diseases, and 57 with gestosis. Abnormally high levels of PAMG were detected in 23 women, 4 of these with clinically uneventful pregnancy. All pregnancies in the group with high concentrations of PAMG had unfavorable outcomes with the following diagnoses at birth: intrauterine hypoxia (14 cases), low-weight babies (5 cases), small-for-date fetuses (2 cases), preterm delivery (1 case), and asphyxia (1 case). Therefore, a high level of PAMG is an indicator of intrauterine disease even in cases when there are no clinical symptoms of pregnancy abnormalities.

[The content of the PAMG-1 protein that binds insulin-like growth factor I (somatomedin C) in the blood serum of diabetic patients]. / Soderzhanie belka PAMG-1, sviazyvaiushchego insulinopodobnyi faktor rosta 1 (somatomedin C), v syvorotke krovi bol'nykh sakharnym diabetom.

The study was undertaken to measure PAMG-1 (PP 12, IGEBP-1) that is insulin-like growth factor binding protein in blood sera of diabetic patients. We could show a stable significant (10-fold or more in 54% of patients) increase of PAMG-1 concentrations in patients suffering with insulin-dependent diabetes and a moderate (1.5-2 times) increase of PAMG-1 levels in 80% of patients suffering with non-insulin-dependent diabetes. Significant individual differences of DAMG-1 serum concentrations were also demonstrated (10-230 mg/ml in insulin-dependent and 0-360 ng/ml in non-insulin-dependent diabetes).

[Histochemical and clinical-diagnostic study of placental alpha 1-microglobulin using monoclonal antibodies]. / Gistokhimicheskoe i kliniko-diagnosticheskoe izuchenie platsentarnogo alpha 1-mikroglobulina s pomoshch'iu monoklonal'nykh antitel.

Five stable hybridomas against placental protein PAMG-1 were obtained. Monoclonal antibodies were not cross reactive with other placental proteins and human serum albumin. The content of PAMG-1 during a physiological pregnancy increased to 40 ng/ml. 23 women with repeated abortions had an increased content of PAMG-1.

[Isolation and analysis of monoclonal antibodies against various gangliosides]. / Poluchenie i issledovanie monoklonal'nykh antitel protiv nekotorykh gangliozidov.

Female BALB/c mice were immunized with human melanoma (Mewo) cells containing ganglioside GD3 as a surface antigen. Immune splenocytes were fused with syngeneic P3-X63.Ag 8 myeloma cells. Antibodies produced by hybrid clones were analyzed by solid phase immunoassay. B, C, D and Q clones producing antibodies against Raja clavata brain gangliosides were obtained. Monoclonal B and C antibodies bound monosialogangliosides. Monoclonal D antibody bound a number of gangliosides but reacted predominantly with GD1a. Monoclonal Q antibody reacted selectively with GQ1c. It is assumed that ganglioside GQ1c is expressed on the melanoma cell surface and may be found only in the early stage of ontogenesis of high vertebrates.

[Pathology of mitosis after cellular recovery from a metaphase block]. / Patologiia mitoza posle vykhoda kletok iz metafaznogo bloka.

The experiments in Chinese hamster fibroblast-like cells have shown that the c-mitosis inducing agents (colchicine, colcemid and low temperature) produce evident stathmokinetic and radiomimetic effects. By the reversibility moment of the former the latter becomes apparent, manifesting in bridge accumulation. This form of pathology is likely to be caused by the disturbance of the cellular nucleoprotein metabolism in c-mitosis.

[Mechanisms of the reversibility of the stathmokinetic response induced by suboptimal temperature]. / Mekhanizmy obratimosti statmokineticheskoi reaktsii, vyzvannoi suboptimal'noi temperaturoi.

Autoradiographic research with leucine-3H showed that the reversibility of the statmokinetic reaction induced by the suboptimal temperature (21 degrees C) did not require any additional protein synthesis and therefore was not connected with the neoformation of the microtubules. Normalization of the mitotic rate was delayed in the presence of copper ions preventing polymerization of microtubules. These data suggest that repolymerization of microtubules subunits was the principal way of mitosis recovery after the action of suboptimal temperature.