Incidence of microplastic contamination in fishes of the Ramsar Wetland, Loktak - The world's only floating lake from the Indian Himalayan region.

Microplastics are ubiquitous, and their widespread prevalence in the ecosphere has generated concerns about their potential effects on terrestrial and aquatic organisms. However, studies pertaining to ecologically sensitive freshwater ecosystems, such as Ramsar wetlands, is scarce. Therefore, the study was conducted in Loktak, the world's only floating lake, and one of the largest wetland in the Indian Himalayan region. The wetland's degradation and pollution have resulted its inclusion in the Montreux Record, underscoring the need for studying this eco-sensitive freshwater system. This work investigated the (i) abundance, morphotype and size of microplastics in fish, and (ii) chemical composition of the microplastics consumed and accumulated in the fish of Loktak lake. Fish samples representing eight species were collected and analyzed for microplastics. Results revealed that ∼91% of the sampled fish ingested microplastics. Fragment was identified as the predominant morphotype (∼82%). Plastic polymers including polyamide (PA), polystyrene (PS), polycarbonate (PC) and carboxymethyl cellulose (CMC) were detected. The occurrence of heavy metals - chlorine (Cl), palladium (Pd), sodium (Na), zinc (Zn), lead (Pb) and copper (Cu) suggests their adhesion on the microplastics. The occurrence of microplastics in fish indicates pollution in the lake and poses a potential health risks to humans through consumption. Therefore, implementing comprehensive management approaches is imperative to mitigate this emerging pollution and uphold the ecological integrity of the Ramsar site. Substantial information on microplastics and their potential human exposure through fish consumption, particularly in the Indian Himalayan region, remains to be assessed, underscoring the need for extensive study.

Geochemical fractionation of iron in paper industry and municipal landfill soils: Ecological and health risks insights.

Industrial processes and municipal wastes largely contribute to the fluctuations in iron (Fe) content in soils. Fe, when present in unfavorable amount, causes harmful effects on human, flora, and fauna. The present study is an attempt to evaluate the composition of Fe in surface soils from paper mill and municipal landfill sites and assess their potential ecological and human health risks. Geochemical fractionation was conducted to explore the chemical bonding of Fe across different fractions, i.e., water-soluble (F1) to residual (F6). Different contamination factors and pollution indices were evaluated to comprehend Fe contamination extent across the study area. Results indicated the preference for less mobile forms in the paper mill and landfill, with 26.66% and 43.46% of Fe associated with the Fe-Mn oxide bound fraction (F4), and 57.22% and 24.78% in the residual fraction (F6). Maximum mobility factor (MF) of 30.65% was observed in the paper mill, and 80.37% in the landfill. The enrichment factor (EF) varied within the range of 20 < EF < 40, signifying a high level of enrichment in the soil. The individual contamination factor (ICF) ranged from 0 to >6, highlighting low to high contamination. Adults were found to be more vulnerable towards Fe associated health risks compared to children. The Hazard Quotient (HQ) index showed the highest risk potential pathways as dermal contact > ingestion > inhalation. The study offers insights into potential Fe contamination risks in comparable environments, underscoring the crucial role of thorough soil assessments in shaping land use and waste management policies.

Phytoremediation of nickel and zinc using Jatropha curcas and Pongamia pinnata from the soils contaminated by municipal solid wastes and paper mill wastes.

The primary source of soil pollution is a complex mixture of numerous inorganic and organic compounds (including chlorinated compounds, nutrients, and heavy metals, etc.). The presence of all of these compounds makes remediation and cleanup difficult. In this study, the phytoremediation ability of Jatropha curcas and Pongamia pinnata was tested to remove nickel (Ni) and Zinc (Zn) from paper mill and municipal landfill contaminated soils, to understand the uptake potential and to estimate the accumulation pattern of Ni and Zn in the vegetative parts of the plant. The experiments were carried out in pots (3 kg capacity) and the different combinations of soil were made by mixing the contaminated soil with a reference soil (forest soil) as T0, T25, T50, T75 and T100. The plant biomass, chlorophyll content, proline, nitrate reductase activity and metal removal efficiency (%)were determined after 120 DAS (i.e., the days after sowing). The results of the study showed that with increasing metal stress, there is a reduction in the above-ground biomass content in both the plant species with a slightly less impact on the root biomass. Over a period of 4 months, J. curcas and P. pinnata removed 82-86% and 93-90% Ni, respectively. The removal of Zn was significantly less as compared to Ni as most of the Zn remained in the belowground part (roots) and in the soil. Besides, the phytostabilization capacities of the plants were calculated on the basis of their tolerance index (TI), bioaccumulation factor (BAF) and translocation factor (TF). The low BAF and TF values with increasing heavy metals (HMs) content indicates its higher phytostabilization capacity in the root and rhizospheric region as compared to phytoaccumulation.

Assessment of mobility and environmental risks associated with copper, manganese and zinc in soils of a dumping site around a Ramsar site.

This study reports the environmental fate and ecological hazard of the three heavy metals (HMs), viz. copper (Cu), manganese (Mn) and zinc (Zn) in soil influenced by municipal solid waste (MSW) dumping. The experimental site is situated in the vicinity of Deepor Beel, a Ramsar site located in Guwahati, India. This study assessed (i) the distribution pattern of Cu, Mn and Zn in six geochemical fractionations; (ii) the mobility, bioavailability and toxicity of Cu, Mn and Zn based on eight contamination and ecological indices, and (iii) the impact of Cu, Mn and Zn on soil quality. Altogether, 18 soil samples were collected and analysed from the study site using stratified random sampling. Pollution indices and multivariate statistics were applied on the data to identify the level and source of analysed HMs. Sequential extraction has revealed that the binding strength of Cu, Mn and Zn had a uniform trend. Mobility and potential bioavailability of studied HMs were in the order Mn > Cu > Zn. Analysed HMs were dominantly associated with non-bioavailable fractions. The observed low values of various contamination factors indicated the lesser contamination load posed by these metals. Conversely, their high enrichment factor and geo-accumulation index values indicated the sources of these metals were anthropogenic. Overall, the pollution and ecological indices registered lower contamination. Yet, it would be prudent to adopt efficient MSW management strategies for eliminating any future risk emanating out of this dumping site and posing threat to nearby Deepor Beel and its associated flora and fauna.

Microwave-assisted one-pot multi-component reaction: synthesis of novel and highly functionalized 3-(pyranyl)- and 3-(dihydropyridinyl)indole derivatives.

One-pot multi-component reaction of 3-cyanoacetyl indole, aromatic aldehydes and ethyl acetoacetate in the presence of InCl(3)/NH(4)OAc under microwave irradiation for 2-7 min afforded novel and highly functionalized 3-(pyranyl)- and 3-(dihydropyridinyl)indole derivatives, respectively, in good yield.

Intramolecular 1,3-dipolar cycloaddition reactions in the synthesis of complex annelated quinolines, α-carbolines and coumarins.

In this study, we report the synthesis of several novel dihydroisoxazole-, tetrahydroisoxazole- and dihydropyrazole-fused pyrido[2,3-b]quinolines, α-carbolines, and pyrido[2,3-c]coumarins, respectively, from simple precursors and by exploring intramolecular 1,3-dipolar cycloaddition reactions involving nitrile oxides, nitrones, and nitrile imines as 1,3-dipoles.

Synthesis of 5-alkylated barbituric acids and 3-alkylated indoles via microwave-assisted three-component reactions in solvent-free conditions using Hantzsch 1,4-dihydropyridines as reducing agents.

Reaction of barbituric acids with aldehydes and dihydropyridines in one pot under microwave (MW) irradiation in the absence of solvent, affords 55­82% of the 5-benzylated barbituric acids. Use of alkyl nitriles or barbituric acids with indole-3-aldehyde and dihydropyridine (DHP) afforded 3-alkylated indoles in 57­76 % yield. In each case DHPs are converted to pyridines.