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OBJECTIVE: This study aimed to evaluate the effect of ultrasonic activation of etch-and-rinse and self-etch adhesive systems on the bond strength of resin cement to irradiated root dentin. MATERIALS AND METHODS: Eighty human maxillary anterior teeth were distributed into 8 groups (n = 10), according to the type of adhesive system used (etch-and-rinse and self-etch), the ultrasonic activation of the adhesive systems, and the dentin condition (irradiated or non-irradiated - 70 Gy). Endodontic treatment was performed followed by fiberglass post-space preparation. After fiberglass posts' luting, the roots were transversely sectioned on dentin discs and submitted to the push-out bond strength test (0.5 mm/min). The fractured specimens were analyzed under a stereomicroscope and Scanning Electron Microscope (SEM) for failure mode classification. One of the dentin discs was analyzed under SEM to evaluate the characteristics of the adhesive interface. RESULTS: Irradiated specimens had lower bond strength than non-irradiated specimens (P < 0.0001). Ultrasonic activation of both adhesive systems increased the bond strength of the resin cement to irradiated dentin (P < 0.0001). Radiotherapy significantly affected the failure mode in the middle (P = 0.024) and apical thirds (P = 0.032) (adhesive failure). CONCLUSION: Non-irradiated specimens had a more homogeneous adhesive interface. When ultrasonically activated, both adhesive systems showed a greater number of resinous tags, regardless of the dentin condition. CLINICAL RELEVANCE: Ultrasonic activation of adhesive systems is a feasible strategy to enhance fiberglass posts retention in oncological patients.
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Colagem Dentária , Dentina , Teste de Materiais , Microscopia Eletrônica de Varredura , Técnica para Retentor Intrarradicular , Cimentos de Resina , Humanos , Cimentos de Resina/química , Colagem Dentária/métodos , Dentina/efeitos da radiação , Técnicas In Vitro , Adesivos Dentinários/química , Análise do Estresse Dentário , Propriedades de Superfície , Raiz Dentária/efeitos da radiação , Ultrassom , Condicionamento Ácido do Dente , Incisivo , Vidro/químicaRESUMO
This study evaluated the hardness of a composite resin used for root reinforcement, considering the light-curing time, root canal region and ageing due to long-term storage. Twenty incisor roots were reinforced using composite resin, varying the photopolymerisation time (40 or 120 s). Following fibre post cementation, the roots were transversely sectioned into coronal, middle and apical regions. Composite hardness was measured initially and after 18 months of water storage. Data underwent repeated measures analysis of variance and Tukey's post hoc tests. The factors 'light-curing time', 'root region' and 'ageing' affected the hardness. Significant interactions were observed between 'light-curing time × root region' and 'ageing × light-curing time'. Regardless of time, resin hardness in the apical region was lower. After ageing, hardness in the coronal and middle regions decreased when the light-curing time was 40 s, while no significant effect on hardness was noted with a light-curing time of 120 s.
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OBJECTIVES: To assess the growth of a multispecies biofilm on root canal dentin under different radiotherapy regimens. MATERIALS AND METHODS: Sixty-three human root dentin cylinders were distributed into six groups. In three groups, no biofilm was formed (n = 3): NoRT) non-irradiated dentin; RT55) 55 Gy; and RT70) 70 Gy. In the other three groups (n = 18), a 21-day multispecies biofilm (Enterococcus faecalis, Streptococcus mutans, and Candida albicans) was formed in the canal: NoRT + Bio) non-irradiated + biofilm; RT55 + Bio) 55 Gy + biofilm; and RT70 + Bio) 70 Gy + biofilm. The biofilm was quantified (CFUs/mL). Biofilm microstructure was assessed under SEM. Microbial penetration into dentinal tubules was assessed under CLSM. For the biofilm biomass and dentin microhardness pre- and after biofilm growth assessments, 45 bovine dentin specimens were distributed into three groups (n = 15): NoRT) non-irradiated + biofilm; RT55 + Bio) 55 Gy + biofilm; and RT70 + Bio) 70 Gy + biofilm. RESULTS: Irradiated specimens (70 Gy) had higher quantity of microorganisms than non-irradiated (p = .010). There was gradual increase in biofilm biomass from non-irradiated to 55 Gy and 70 Gy (p < .001). Irradiated specimens had greater reduction in microhardness after biofilm growth. Irradiated dentin led to the growth of a more complex and irregular biofilm. There was microbial penetration into the dentinal tubules, regardless of the radiation regimen. CONCLUSION: Radiotherapy increased the number of microorganisms and biofilm biomass and reduced dentin microhardness. Microbial penetration into dentinal tubules was noticeable. CLINICAL RELEVANCE: Cumulative and potentially irreversible side effects of radiotherapy affect biofilm growth on root dentin. These changes could compromise the success of endodontic treatment in oncological patients undergoing head and neck radiotherapy.
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Biofilmes , Candida albicans , Cavidade Pulpar , Dentina , Enterococcus faecalis , Streptococcus mutans , Biofilmes/efeitos da radiação , Dentina/microbiologia , Dentina/efeitos da radiação , Humanos , Cavidade Pulpar/microbiologia , Cavidade Pulpar/efeitos da radiação , Candida albicans/efeitos da radiação , Animais , Enterococcus faecalis/efeitos da radiação , Streptococcus mutans/efeitos da radiação , Bovinos , Microscopia Eletrônica de Varredura , Dureza , Microscopia Confocal , Dosagem RadioterapêuticaRESUMO
This study investigated the effect of the timing of primary endodontic treatment and dosage of radiotherapy on the remaining filling material (RFM) during endodontic reintervention. 60 single-rooted human mandibular premolars were distributed into five groups (n = 12), according to the timing and dosage of radiation (55Gy or 70Gy): NegativeCG-non-irradiated teeth; Endo-pre-RT55/70-obturation before irradiation (55Gy or 70Gy); Endo-post-RT55/70-obturation and reintervention after irradiation (55Gy or 70Gy). Roots were cleaved and analysed under stereomicroscope and Scanning Electron Microscope to quantify (%) the RFM. Experimental groups had a significantly greater amount (p < 0.05) of RFM in the middle and apical thirds than the control group, except for Endo-pre-RT55 in the middle third (p < 0.0001). The apical third had greater amount of RFM (p < 0.05). Radiation therapy, before and after primary endodontic treatment, increased the amount of RFM, regardless of the dose delivered. When necessary, reintervention preferably must be performed before radiation therapy.
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Materiais Restauradores do Canal Radicular , Humanos , Materiais Restauradores do Canal Radicular/uso terapêutico , Fatores de Tempo , Tratamento do Canal Radicular/métodos , Dente Pré-Molar , Obturação do Canal Radicular/métodos , Microscopia Eletrônica de Varredura , Dosagem Radioterapêutica , Retratamento/métodosRESUMO
INTRODUCTION: To evaluate the push-out bond strength (POBS) of AH Plus sealer to root dentin and the adhesive interface quality after calcium hydroxide (Ca(OH)2) intracanal dressing removal with different final irrigation protocols. METHOD: After root canal instrumentation and irrigation, 40 root canals were filled with Ca(OH)2 and sealed. After 14 days, the specimens were randomly distributed according to the irrigation protocols for Ca(OH)2 removal (n = 10): GH2O (control) - distilled water; GNaOCl - 1% NaOCl; GEDTA - 17% EDTA; GEDTA + NaOCl - 17% EDTA + 1% NaOCl. The root canals were filled with AH Plus sealer and gutta-percha. After 7 days, the roots were sectioned into dentin slices and submitted to POBS test and analysis of the adhesive interface under scanning electron microscope. The POBS data were statistically evaluated (analysis of variance and Tukey test). The Kruskal-Wallis and Mann-Whitney tests were used to analyze the adhesive interface (α = 0.05). RESULTS: GH2O, GNaOCl, and GEDTA + NaOCl had similar POBS values, with higher values on the apical third, in comparison with other thirds (P < .05). A homogeneous and free-of-gaps adhesive interface was observed for GH2O, GNaOCl, and GEDTA + NaOCl, with difference between GH2O and GEDTA (P < .05). GH2O and GEDTA + NaOCl presented higher sealer tags formation (P < .05). CONCLUSION: The final rinse with EDTA for Ca(OH)2 dressing removal had a negative effect on the POBS of the filling material to root dentin. The use of EDTA followed by NaOCl had results similar to the distilled water, providing uniform and free-of-gaps adhesive interface, and a higher number of sealer tags.
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Hidróxido de Cálcio , Colagem Dentária , Dentina , Resinas Epóxi , Materiais Restauradores do Canal Radicular , Irrigantes do Canal Radicular , Irrigação Terapêutica , Hidróxido de Cálcio/química , Humanos , Dentina/efeitos dos fármacos , Materiais Restauradores do Canal Radicular/química , Resinas Epóxi/química , Colagem Dentária/métodos , Irrigação Terapêutica/métodos , Cavidade Pulpar/efeitos dos fármacos , Microscopia Eletrônica de Varredura , Hipoclorito de Sódio/química , Ácido Edético , Preparo de Canal Radicular/métodos , Análise do Estresse Dentário , Teste de MateriaisRESUMO
INTRODUCTION: This study assessed the effect of intentional foraminal enlargement on the foramen and the apical root canal morphology. METHOD: Sixty mesial roots of mandibular molars were scanned by micro-computed tomography. Their apical foramina were photographed with a stereomicroscope before and after preparation. Three groups were formed (n = 20) according to the working length (WL). G-1: foramen - 1 mm; G0: foramen; and G+1: foramen + 1 mm. Each group originated 2 subgroups (n = 10): G-1: Buchanan's patency (size 10 K-type file) and foraminal debridement (sizes 20, 25 and 30 K-type files); G0 and G+1: rotary foraminal enlargement (ProDesign S size 25/.08) or reciprocating foraminal enlargement (R25). The area, perimeter, transportation, and noninstrumented walls of the foramen were evaluated. The root canal transportation and the centering index of preparation at 1, 3 and 5 mm from the foramen were also assessed. Data were compared statistically (α = 5%). RESULTS: The instruments used at the foramen and 1 mm beyond promoted foraminal enlargement and transportation. Regarding NIW, there was no difference between mechanized foraminal enlargements performed at the foramen or 1 mm beyond, similar to the manual foraminal debridement group (P > .05). There was no difference in transportation and centralization at 1-, 3-, and 5-mm apical levels, regardless of the instrumentation systems. CONCLUSION: Mechanical preparation at the foramen, or 1 mm beyond, resulted in foraminal enlargement, transportation and were not able to touch all root canal walls that delimit the foramen.
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Cavidade Pulpar , Dente Molar , Preparo de Canal Radicular , Ápice Dentário , Microtomografia por Raio-X , Humanos , Cavidade Pulpar/diagnóstico por imagem , Cavidade Pulpar/anatomia & histologia , Preparo de Canal Radicular/instrumentação , Preparo de Canal Radicular/métodos , Dente Molar/diagnóstico por imagem , Ápice Dentário/diagnóstico por imagem , Ápice Dentário/anatomia & histologia , Mandíbula/diagnóstico por imagem , Mandíbula/anatomia & histologiaRESUMO
To evaluate the effect of ultrasonic activation of the endodontic sealer on its intratubular penetration and bond strength to irradiated root dentin. Forty human teeth were distributed into 4 groups (n = 10), according to the radiation therapy (RT) exposure-70 Gy-and ultrasonic activation (UA) of the endodontic sealer: RT/UA-irradiated teeth and sealer UA; RT/no-UA-irradiated teeth and no sealer UA; no-RT/UA-non-irradiated teeth and sealer UA and no-RT/no-UA-non-irradiated teeth and no sealer UA. Push-out bond strength test was performed in a Universal Testing Machine. Failure modes and adhesive interface were analyzed under Scanning Electron Microscopy. The data were statistically compared (two-way-ANOVA and posthoc Games-Howell test; Fisher's exact test - α = 5%). The different experimental conditions (radiation and UA) and the root third had a significant effect on push-out bond strength, and the interaction of these factors was significant (p < 0.05). UA of the sealer significantly increased its bond strength to both irradiated and non-irradiated dentin (p < 0.05). The irradiated groups mostly presented adhesive-type failure of the sealer (p < 0.01). Regardless of the irradiation, the ultrasonically activated groups showed a more homogeneous adhesive interface, with the presence of sealer tags in greater density and depth. Ultrasonic activation enhanced the intratubular penetration and the bond strength of the endodontic sealer to irradiated dentin. The impact of ultrasonic activation of the endodontic sealer on teeth undergoing radiotherapy is a gap in the scientific literature that needs to be bridged.
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Colagem Dentária , Dentina , Teste de Materiais , Microscopia Eletrônica de Varredura , Materiais Restauradores do Canal Radicular , Humanos , Materiais Restauradores do Canal Radicular/química , Colagem Dentária/métodos , Dentina/efeitos da radiação , Técnicas In Vitro , Análise do Estresse Dentário , Propriedades de Superfície , Ultrassom , Raiz Dentária/efeitos da radiaçãoRESUMO
This study evaluated changes in the root canal length (RCL) and the accuracy of the electronic apex locator (EAL) during the different stages of endodontic treatment and retreatment. Fifty-six mesial root canals of mandibular molars were selected. The actual root canal length (AL) of the canals was obtained by inserting a size 15 hand file up to the apical foramen, under magnification. The electronic lengths were obtained at the "APEX" mark of Root ZX II, using an alginate model. Both measurements were performed at three different stages of the initial root canal treatment-unflared, flared, and concluded-and at two stages of retreatment, after achieving patency and repreparation. Data were statistically analyzed and the significance level established was 5%. All stages produced a significant reduction in the AL (p < 0.05). The greatest variation was observed between the unflared-flared stages (0.2 mm) and between concluded-patency stages (0.09 mm), with no difference between them (p > 0.05). The accuracy of Root ZX II was negatively affected after achieving patency, presenting statistically significant difference compared to the other stages (p < 0.05). A significant reduction in the RCL was observed along the different stages of endodontic treatment and retreatment. The EAL was accurate to measure the root canals in most stages, except after achieving patency for endodontic retreatment. Determining and monitoring the RCL is an essential step towards a favorable prognosis, since it reduced along the different stages evaluated. Root ZX II was not accurate for endodontic retreatment.
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Cavidade Pulpar , Preparo de Canal Radicular , Humanos , Odontometria , Eletrônica , RetratamentoRESUMO
The interaction of sodium hypochlorite (NaOCl) and chlorhexidine gluconate (CHX) produces an orange-brown precipitate. The present study evaluated the influence of distilled water (H2 O) in different irrigation protocols designed to prevent the formation of precipitate with NaOCl and CHX. Fifty canine teeth were instrumented and split longitudinally. The canal was examined with a stereomicroscope and photographed by canal-thirds. The tooth halves were repositioned and distributed randomly into five groups, according to the final irrigation protocol (n = 10): G1 (control)-Ethylenediaminetetraacetic acid (EDTA) + NaOCl + CHX, conventional irrigation (CI); G2-EDTA + NaOCl + CHX, activated with passive ultrasonic irrigation (PUI); G3-EDTA (PUI) + NaOCl (PUI) + H2 O (CI) + CHX (PUI); G4-EDTA + NaOCl + H2 O + CHX (PUI); G5-EDTA (PUI) + NaOCl (PUI) + H2 O (continuous ultrasonic irrigation [CUI]) + CHX (PUI). The specimens were evaluated with a stereomicroscope and scanning electron microscope (SEM). Energy dispersive x-ray spectroscopy analysis was performed to identify the elemental profile of the irrigated canal walls. The images were scored according to the extensiveness of precipitate. Data were analyzed (Kruskal-Wallis test, α = 5%). Under the stereomicroscope, G1 had significantly higher scores than all the other groups in all canal-thirds (p < .05). All four experimental groups showed similar scores (p > .05). There were no significant differences in precipitate formation among root-thirds in intragroup analysis (p > .05). Upon SEM examination, overall, only G5 had lower scores than G1 (p < .05). Analysis by canal-thirds showed no significant difference among groups and among canal-thirds in the intragroup analysis (p > .05). G1 showed high Cl peaks. In-between irrigation with H2 O activated by CUI is effective in preventing precipitate formation during canal debridement with NaOCl and CHX. RESEARCH HIGHLIGHTS: Continuous ultrasonic irrigation with distilled water was capable to prevent the precipitate formation. The precipitate can be classified as a chemical smear layer.
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Clorexidina , Hipoclorito de Sódio , Hipoclorito de Sódio/farmacologia , Hipoclorito de Sódio/química , Clorexidina/farmacologia , Clorexidina/química , Ácido Edético , Microscopia Eletrônica de Varredura , Dente Canino , Irrigantes do Canal Radicular/farmacologia , Irrigantes do Canal Radicular/química , Água , Cavidade Pulpar , Preparo de Canal Radicular/métodosRESUMO
The aim of this study was to perform an integrative review to identify the most effective supplementary protocols used after filling material (FM) removal and root canal reinstrumentation, during endodontic reintervention. The literature search was performed on the electronic databases PubMed and Latin American and Caribbean Health Sciences Literature (LILACS), using a combination of specific scientific descriptors. Selection criteria accepted articles published in English, Spanish and Portuguese languages, up to August 2021, involving in vitro and ex vivo studies. After applying the eligibility criteria, 46 articles were included for qualitative analysis. The tool for qualitative analysis of quasi-experimental studies of the Joanna Briggs Institute was used to determine the risk of bias of the included articles. The selected articles provided important data regarding the following supplementary protocols: sonic and ultrasonic activation of the irrigating solution; the use of ultrasonic inserts, XP-endo system instruments, and photon-induced photoacoustic streaming for mechanical debridement; and new devices as GentleWave system, and the self-adjusting file. Overall, all supplementary protocols demonstrated efficacy in removing the remaining FM. The implementation of various supplementary protocols can effectively remove the remaining FM from the root canal walls, although complete removal is not always achieved. However, it was not possible to determine the most effective protocol. Conversely, their association can enhance remaining FM removal. Remaining FM attached to root canal walls hinders proper chemical-mechanical preparation during endodontic reintervention. Supplementary protocols optimize the remaining FM removal, enhancing cleaning and disinfection of root canal.
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Materiais Restauradores do Canal Radicular , Obturação do Canal Radicular , Preparo de Canal Radicular/métodos , Microtomografia por Raio-X , Guta-Percha , Cavidade PulparRESUMO
BACKGROUND: The literature is scanty regarding the effect of radiation therapy (RT) on the mechanical properties of immature permanent teeth. AIM: To evaluate the effect of RT on the fracture resistance of simulated immature teeth submitted to different types of root reinforcement. DESIGN: Sixty-four human teeth simulating the Cvek stage 3 of root development were distributed into eight groups (n = 8), according to exposure or not to RT (70 Gy) and the root reinforcement method: Group NR (control)-no reinforcement/no RT; Group NR + RT (control)-no reinforcement/RT; Group PO-tricalcium silicate-based cement (TS) apical plug/canal obturation/no RT; Group PO + RT-TS apical plug/canal obturation/RT; Group TS-canal filling with TS/no RT; Group TS + RT-canal filling with TS/RT; Group FP-TS apical plug/fibreglass post/no RT; and Group FP + RT-TS apical plug/fibreglass post/RT. Fracture resistance was determined using a universal testing machine (0.5 mm/min). RESULTS: In the intergroup comparison, nonirradiated teeth had higher fracture resistance (p < .05). Groups FP and FP + RT had higher fracture resistance (p < .001). CONCLUSION: Radiotherapy affected the fracture resistance of simulated immature teeth. Reinforcement with fibreglass posts increased the fracture resistance, regardless of the radiation.
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Materiais Restauradores do Canal Radicular , Fraturas dos Dentes , Humanos , Compostos de Cálcio , Raiz Dentária , SilicatosRESUMO
This study evaluated the effect of heating on the physicochemical properties and surface changes of tricalcium silicate sealers. Three tricalcium silicate root canal sealers (Bio-C Sealer, BioRoot-RCS, EndoSequence BC Sealer), and one epoxy resin-based sealer (AH Plus; control) were tested. The effect of heating on setting time (ST) and flowability were assessed according to ANSI/ADA 57 and ISO 6876 standards. Solubility and dimensional change (DC) of the set sealers were evaluated at 24 hours and after 30 days; the pH of the water used in the DC testing was also measured. Tests were repeated with heated sealers in an oven at 100 °C for 1 min. SEM and EDS analysis were performed. Data were analyzed using One-Way ANOVA and Tukey post-hoc tests (α=5%). Heating decreased the ST for AH Plus and EndoSequence (p<0.05). Heating reduced flowability (p<0.05) and increased pH for AH Plus (p<0.05). The solubility of Bio-C (dried specimens) was not in accordance with the ANSI/ADA standard. The solubility of EndoSequence was significantly higher (p<0.05) when it was heated and dried after 30 days. DC of Bio-C (24 h and 30 days), BioRoot-RCS (30 days) and AH Plus (24 h and 30 days) were not in accordance with the standards. SEM and EDS analysis showed significant changes in sealer microstructure after heating. In conclusion, heating decreased the ST and increased the solubility of EndoSequence BC sealer. No significant changes in flowability, DC, and pH were identified for all three tricalcium silicate sealers after heat application. However, all sealers had significant surface changes.
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Materiais Restauradores do Canal Radicular , Materiais Restauradores do Canal Radicular/química , Calefação , Cavidade Pulpar , Compostos de Cálcio/química , Resinas Epóxi/química , Silicatos/química , Teste de MateriaisRESUMO
Objectives: This study was conducted to evaluate and compare the tip and taper compatibility of accessory gutta-percha points (AGPs) with various rotary and reciprocating instruments. Materials and Methods: Using a profile analyzer, tip and taper measurements were taken of 10 AGPs of each of the 14 models available from Odous de Deus and the 4 models available from Dentsply-Maillefer. Diameter measurements were taken at 1-mm intervals, from 3 mm from the tip (D3) to 16 mm. Results: Based on the mean values obtained, 3-dimensional (3D) models of the AGPs were drawn in Autodesk Fusion 360 and superimposed on 3D models of each instrument selected (Mtwo, Reciproc, RaCe, K3, and ProDesign Logic) to determine the compatibility between the instrument and the AGP. Data corresponding to the tips and tapers of the various AGPs, as well as the tip and taper differences between the AGPs and the instruments, were analyzed using descriptive statistics. The tapers of the AGPs were subject to the American National Standards Institute/American Dental Association No. 57 standard. The Odous de Deus extra-long medium and extra-long extra-medium AGPs were shown to be compatible with Mtwo, K3, and ProDesign Logic instruments with taper 0.06 and tip sizes 25 and 30, while the Dentsply fine and fine medium cones were compatible with Mtwo, RaCe, and K3 instruments with conicity of 0.04 and tip sizes 35 and 40. Conclusions: Both the Odous de Deus and Dentsply commercial brands included 2 AGP models with tip (D3) and taper compatibility with Mtwo, RaCe, K3, and/or Prodesign Logic instruments.
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Abstract This study evaluated the effect of heating on the physicochemical properties and surface changes of tricalcium silicate sealers. Three tricalcium silicate root canal sealers (Bio-C Sealer, BioRoot-RCS, EndoSequence BC Sealer), and one epoxy resin-based sealer (AH Plus; control) were tested. The effect of heating on setting time (ST) and flowability were assessed according to ANSI/ADA 57 and ISO 6876 standards. Solubility and dimensional change (DC) of the set sealers were evaluated at 24 hours and after 30 days; the pH of the water used in the DC testing was also measured. Tests were repeated with heated sealers in an oven at 100 °C for 1 min. SEM and EDS analysis were performed. Data were analyzed using One-Way ANOVA and Tukey post-hoc tests (α=5%). Heating decreased the ST for AH Plus and EndoSequence (p<0.05). Heating reduced flowability (p<0.05) and increased pH for AH Plus (p<0.05). The solubility of Bio-C (dried specimens) was not in accordance with the ANSI/ADA standard. The solubility of EndoSequence was significantly higher (p<0.05) when it was heated and dried after 30 days. DC of Bio-C (24 h and 30 days), BioRoot-RCS (30 days) and AH Plus (24 h and 30 days) were not in accordance with the standards. SEM and EDS analysis showed significant changes in sealer microstructure after heating. In conclusion, heating decreased the ST and increased the solubility of EndoSequence BC sealer. No significant changes in flowability, DC, and pH were identified for all three tricalcium silicate sealers after heat application. However, all sealers had significant surface changes.
Resumo Este estudo avaliou o efeito do aquecimento nas propriedades físico-químicas e nas alterações superficiais de cimentos de silicato tricálcico. Três cimentos endodônticos de silicato tricálcico (Bio-C Sealer, BioRoot-RCS, EndoSequence BC Sealer) e um cimento à base de resina epóxi (AH Plus) foram testados para avaliar o efeito do aquecimento no tempo de presa (TP) e escoamento de acordo com as normas ANSI/ADA 57 e ISO 6876. A solubilidade e alteração dimensional (AD) dos cimentos foram avaliadas após 24 horas e 30 dias. O pH da água usada no teste de AD também foi medido. Os testes foram repetidos com cimentos aquecidos em um forno a 100 °C por 1 min. Análises em MEV e EDS foram realizadas. Os dados foram analisados pelos testes de ANOVA-1 via e post hoc de Tukey (α=5%). O aquecimento diminuiu o TP para AH Plus e EndoSequence (p<0,05). O aquecimento reduziu o escoamento (p<0,05) e aumentou o pH do AH Plus (p<0,05). A solubilidade do Bio-C (amostras desidratadas) não estava de acordo com o padrão ANSI/ADA. A solubilidade da EndoSequence foi significativamente maior (p<0,05) quando aquecida e desidratada após 30 dias. As AD de Bio-C (24 h e 30 dias), BioRoot-RCS (30 dias) e AH Plus (24 h e 30 dias) não estavam de acordo com as normas ADA e ISO. A análise em MEV e EDS mostrou alterações significativas na estrutura dos cimentos após aquecimento. Em conclusão, o aquecimento diminuiu o TP e aumentou a solubilidade do cimento EndoSequence BC. Não foram identificadas mudanças significativas no escoamento, AD e pH para os cimentos de silicato tricálcico após aquecimento. Porém, todos os cimentos tiveram alterações significativas na superfície.
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OBJECTIVES: To determine the prevalence of postoperative pain after endodontic treatment using low (LC) and high (HC) concentrations of sodium hypochlorite (NaOCl). MATERIALS AND METHODS: Six databases and the grey literature were searched to identify randomized clinical trials that evaluated postoperative pain after endodontic treatment using NaOCl. NaOCl concentrations were dichotomized into 'LC' (0.5% to 3%) and 'HC' (≥ 5%) and a proportion meta-analysis was applied to determine the postoperative pain prevalence: overall and according to pain intensity and postoperative time. The prevalence of patients using pain control medication was also determined. A significance level of 5% and a random effect model were applied for data analysis. Between-study heterogeneity was assessed by I2 index. Risk of bias (RoB) was assessed using the Cochrane Risk-of-Bias 2.0 tool. The certainty of evidence was assessed using the GRADE approach. RESULTS: Ten studies were included in the review and eight in the meta-analysis. The overall prevalence of postoperative pain was 45% in LC and 39% in HC. The prevalence of pain in LC and HC after 24 h was 25% and 40%, respectively. After 48 h, the prevalence decreased to 10% in LC and 25% in HC. 'Absent pain' was the most prevalent score. The prevalence of patients who used medication was 9% in LC and 15% in HC. Three studies were classified as 'high RoB', five as 'low RoB', and two as 'some concerns'. The certainty of evidence was very low. CONCLUSIONS: The overall prevalence of postoperative pain after endodontic treatment using LC and HC of NaOCl was 45% and 39%, respectively. CLINICAL RELEVANCE: Postoperative pain is common after endodontic treatment using NaOCl, but tends to decrease over time.
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Irrigantes do Canal Radicular , Hipoclorito de Sódio , Humanos , Hipoclorito de Sódio/uso terapêutico , Prevalência , Irrigantes do Canal Radicular/uso terapêutico , Manejo da Dor , Dor Pós-Operatória/tratamento farmacológico , Dor Pós-Operatória/epidemiologiaRESUMO
Few long-term studies assess the discoloration induced by hydraulic calcium silicate-based cement on dental structures. In addition, as far as we know, no long-term study has assessed the discoloration induced by these cement on composite resin. This in vitro study aimed to assess, during a period of two years, the discoloration potential of different hydraulic calcium silicate-based cements (hCSCs) on the enamel/dentin structure and composite resin restoration. A total of 40 enamel/dentin discs were obtained from bovine incisors, and 40 composite resin discs (10 mm in diameter × 2 mm thick) were fabricated. A 0.8 mm-deep cavity was made in the center of each disc and filled with the following hCSCs (n=10): Original MTA (Angelus); MTA Repair HP (Angelus); NeoMTA Plus (Avalon); and Biodentine (Septodont). An initial color measurement was performed (T0 - baseline). After 7, 15, 30, 45, 90, 300 days, and two years, new color measurements were performed to determine the color (ΔE00), lightness (ΔL'), chroma (ΔC'), hue differences (ΔH'), and whiteness index (WID). For enamel/dentin, the ΔE00 was significant among groups and periods (p<0.05). NeoMTA Plus had the greatest ΔE00. The NeoMTA Plus group had the greatest ΔE00 after two years for composite resin. Significant reduction in lightness was observed for all groups after two years (p<0.05). The most significant WID values were observed after 30 days for Biodentine (enamel/dentin) and MTA Repair HP groups (composite resin) (p<0.05). The hCSCs changed the colorimetric behavior of both substrates, leading to greater darkening over time. The Bi2O3 in the Original MTA seems relevant in the short periods of color change assessment.
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Compostos de Cálcio , Resinas Compostas , Animais , Bovinos , Resinas Compostas/efeitos adversos , Resinas Compostas/química , Compostos de Cálcio/efeitos adversos , Silicatos/efeitos adversos , Cimentos Dentários/efeitos adversos , Óxidos , Combinação de Medicamentos , Teste de Materiais , Compostos de Alumínio/efeitos adversos , Cimentos de Resina/efeitos adversosRESUMO
Objectives: This study evaluated the effect of different nanoparticulated zinc oxide (nano-ZnO) and conventional-ZnO ratios on the physicochemical properties of calcium aluminate cement (CAC). Materials and Methods: The conventional-ZnO and nano-ZnO were added to the cement powder in the following proportions: G1 (20% conventional-ZnO), G2 (15% conventional-ZnO + 5% nano-ZnO), G3 (12% conventional-ZnO + 3% nano-ZnO) and G4 (10% conventional-ZnO + 5% nano-ZnO). The radiopacity (Rad), setting time (Set), dimensional change (Dc), solubility (Sol), compressive strength (Cst), and pH were evaluated. The nano-ZnO and CAC containing conventional-ZnO were also assessed using scanning electron microscopy, transmission electron microscopy, and energy-dispersive X-ray spectroscopy. Radiopacity data were analyzed by the 1-way analysis of variance (ANOVA) and Bonferroni tests (p < 0.05). The data of the other properties were analyzed by the ANOVA, Tukey, and Fisher tests (p < 0.05). Results: The nano-ZnO and CAC containing conventional-ZnO powders presented particles with few impurities and nanometric and micrometric sizes, respectively. G1 had the highest Rad mean value (p < 0.05). When compared to G1, groups containing nano-ZnO had a significant reduction in the Set (p < 0.05) and lower values of Dc at 24 hours (p < 0.05). The Cst was higher for G4, with a significant difference for the other groups (p < 0.05). The Sol did not present significant differences among groups (p > 0.05). Conclusions: The addition of nano-ZnO to CAC improved its dimensional change, setting time, and compressive strength, which may be promising for the clinical performance of this cement.
RESUMO
Background: This study aimed to evaluate the pH and calcium ions (Ca2+) release from two tricalcium silicate-based sealers (Sealer Plus BC and Bio-C Pulpecto) through roots of primary teeth. Methods: Forty root canals of primary incisors were prepared and distributed into four groups according to the filling material: GPlusBC (Sealer Plus BC); GBioC (Bio-C Pulpecto); GUltra (Ultracal); and GC (no filled). pH measurement was performed with a digital pH meter, and the Ca2+ release was measured in an atomic absorption spectrophotometer at baseline, 24 h and 1, 2, 3, and 4 weeks later. The data were analyzed using two-way ANOVA and Tukey's post hoc tests. Results: The results did not reveal significant differences between the groups in terms of pH in 24 h, 1-, and 2-weeks' periods. After 4 weeks, there was a significant difference, with the highest mean pH values in GUltra, followed, respectively, by GPlusBC and GBioC. Regarding the Ca2+ release, the GUltra showed greatest mean values at all evaluated times, and the other groups showed no difference between them. Conclusion: Both sealers were able to promote the elevation of the pH and Ca2+ release through roots of primary teeth, which brings favorable properties for their use as a filling material.
RESUMO
The use of fluorescent dyes in microscopy studies is frequent. Therefore, it is important to investigate whether these compounds may alter the physicochemical properties of materials in which they are incorporated to avoid methodological biases. This study evaluated the physicochemical properties of two endodontic sealers containing dry or diluted rhodamine B. Six groups were evaluated: AH Plus and MTA Fillapex in their original composition and mixed with 0.1% dry or diluted rhodamine. Push-out test was applied to assess the bond strength to root dentin. ANSI-ADA No. 57 and ISO No. 6876:2012 specifications were followed to evaluate flowability, setting time, and solubility of the sealers. pH changes were assessed after 24 h, 7, and 30 days. Intergroup comparisons were analyzed by ANOVA complemented by Tukey's post-hoc test; comparisons among periods were analyzed by Wilcoxon and Friedman tests. The incorporation of dry rhodamine decreased the bond strength and prolonged the setting time of AH Plus. The incorporation of diluted rhodamine decreased the setting time and increased the flowability of MTA Fillapex. Diluted rhodamine promoted pH reduction of AH Plus after 24 h and dry rhodamine after 7 days. Rhodamine incorporation promoted pH reduction after 30 days for MTA Fillapex. Solubility was not affected. In conclusion, the incorporation of diluted rhodamine changed the properties of MTA Fillapex, and the incorporation of dry rhodamine changed the properties of AH Plus. Previous dilution of rhodamine did not severely compromise the physicochemical properties of AH Plus and may be suggested to assess its penetrability.
Assuntos
Materiais Restauradores do Canal Radicular , Materiais Restauradores do Canal Radicular/química , Resinas Epóxi/química , Compostos de Cálcio/química , Óxidos/química , Rodaminas , Solubilidade , Combinação de Medicamentos , Silicatos/química , Teste de MateriaisRESUMO
The present study reported a method for inducing incomplete root fracture in human extracted teeth for the purpose of evaluating the merits of different diagnostic imaging techniques. Thirty-five single-rooted teeth were inspected under magnification and transillumination to exclude previously fractured teeth. Tooth crowns were removed, and the root canals were prepared up to the ProTaper Next X4 (40.06) file. Each root was lined with wax and embedded in a polystyrene resin block. The setup was attached to a universal testing machine for pressing a customized conical wedge (diameter at tip: 0.6 mm; taper: 0.2 mm/mm) into the instrumented canal with a 2 kN load at 5 mm/min. The machine was programmed to stop after a sudden 10% drop in loading force. Each specimen was removed from the resin block and inspected under × 20 magnification and transillumination to identify the fracture characteristics (pattern, surfaces and root-third affected). The gap width of each specimen was measured at different locations along the fracture line. The protocol induced incomplete vertical root fractures in all specimens. Fracture widths were < 100 µm in all specimens (mean gap width: 34.9 µm). The proposed methodology was successful in inducing incomplete vertical root fractures with characteristics that resemble the clinical presentation of these conditions. The method is easy to execute, highly reproducible and helps to minimize bias in laboratory studies that aims to mimic vertical root fractures.