Extrato de alho no controle in vitro e in vivo da antracnose da videira / Garlic extract in the in vitro and in vivo control of anthracnose of grapevine

O objetivo deste trabalho foi avaliar a eficiência in vitro e in vivo do extrato bruto de alho no controle da antracnose da videira (Elsinoe ampelina). No primeiro experimento in vitro, adicionaram-se doses de 0, 5, 10, 15, 20, 25 ou 30 mL L-1 de extrato bruto de alho em meio batata-dextrose-ágar (BDA) antes da esterilização em autoclave e em meio fundente. Após 3, 5, 7 e 9 dias de incubação a 24 ± 2ºC e fotoperíodo de 16 horas, mensurou-se o crescimento micelial de E. ampelina. No segundo experimento in vitro, repetiu-se a metodologia de adição de extrato em meio fundente acrescentando 2,5 mL L-1 de óleo vegetal e uma testemunha absoluta somente com BDA. Avaliação da germinação de E. ampelina foi realizada após duas horas e após quatro horas de incubação a 24ºC e luz constante. O delineamento experimental utilizado para os experimentos in vitro foi inteiramente casualizado, com quatro repetições e parcela experimental constituída por uma placa de Petri. No experimento a campo, logo após a poda da videira cv. Isabel pulverizou-se semanalmente, sobre as folhas da planta, as doses de extrato bruto de alho acrescida do óleo, exceto na testemunha absoluta (sem tratamento). A partir dos primeiros sintomas da antracnose da videira, avaliou-se a severidade que foi expressa em área abaixo da curva do progresso da doença (AACPD). O delineamento foi em blocos ao acaso com cinco repetições. Constatou-se que o extrato bruto de alho reduziu o crescimento micelial do patógeno, principalmente ao adicioná-lo em meio de cultura, antes da esterilização, quando expressou o máximo potencial antifúngico. Ao adicionar o óleo vegetal às doses de extrato, constatou-se inibição total nas doses de 25 ou 30 mL L-1. Este efeito aditivo entre esses compostos também foi constato no teste de germinação de E. ampelina. Em condições de campo, o extrato bruto de alho reduziu a AACPD em 83,59% na dose de 25 mL L-1.

The aim of this study was to evaluate the in vitro and in vivo efficacy of the crude extract of garlic in controlling anthracnose of grapevine (Elsinoe ampelina). In the first experiment in vitro, 0, 5, 10, 15, 20, 25 or 30 mL L-1 crude extract of garlic were added to potato-dextrose-agar medium (PDA) before autoclaving for sterilization and to melting media. After 3, 5, 7 and 9 days of incubation at 24 ± 2ºC and photoperiod of 16 hours, the mycelial growth of E. ampelina was measured. In the second experiment in vitro, we repeated the methodology of adding the extract to the melting medium plus 2.5 mL-1 of vegetable oil and an absolute control with PDA only. After two and four hours of incubation at 24ºC and constant light, germination of E. ampelina was assessed. The experimental design for in vitro experiments was completely randomized with four replicates and the experimental plot consisted of a Petri dish. In the field experiment, after pruning of the grapevine cv. Isabel, the levels of crude extract of garlic plus oil were weekly sprayed on the leaves of the plant, except on the absolute control (no treatment). From the first symptoms of anthracnose of grapevine, the severity that was expressed as area under the disease progress curve (AUDPC) was evaluated. The design was in randomized blocks with five replicates. The crude extract of garlic reduced the mycelial growth of the pathogen, especially when added to the culture medium prior to sterilization, when it expressed its maximal antifungal potential. When the vegetable oil was added to the extract levels, there was complete inhibition at the levels 25 or 30 mL L-1. This additive effect of these compounds was also noted for the germination test of E. ampelina. Under field conditions, the crude extract of garlic reduced the AUDPC by 83.59% at 25 mL L-1.

Existe uma correlação entre a melhora clínica e retorno ao trabalho nos pacientes, funcionários públicos, submetidos à cirurgia de hérnia de disco lombar / Is there a correlation between clinical improvement and return to work in patients, civil servants, who underwent surgery for lumbar disc herniation

Objetivo. Analisar a taxa de retorno ao trabalho em pacientes, funcionários públicos, submetidos à cirurgia para hérnia discal lombar.Métodos. A amostra foi formada por 30 pacientes. O nível de dor foi quantificado através da Escala Visual Analógica no pré-operatório e no 3º mês pós-operatório, quando também foi avaliada a situação do trabalho através da Escala de Trabalho de Denis. Foi comparada a dor do pré e do pósoperatório, a melhora clínica e o retorno ao trabalho.Resultados. A média das idades foi de 47,42 anos, o gênero feminino prevaleceu em 54%. A média da dor no pré-operatório foi de 9,11 e no pós-operatório foi de 3,11 e 77% dos pacientes retornaram ao trabalho prévio. Houve correlação moderada entre a dor no pós-operatório e a condição de trabalho.

Polysomnographic respiratory findings in patients with Arnold-Chiari type I malformation and basilar invagination, with or without syringomyelia: preliminary report of a series of cases.

Patients with craniocervical disorders (CCD) show a wide variety of symptoms and signs suggesting cerebellar and/or high cervical lesion. The anatomic localization of respiratory centers and their possible injury may explain the presence of respiratory disturbances in these diseases. The aim of this preliminary study was to evaluate the polysomnographic findings in a group of patients with CCD, most of them with Arnold-Chiari malformation type I, since sleep apnea has been referred to in isolated cases in the literature. Eleven patients (seven females and four males) with CCD diagnosed by magnetic resonance imaging referred from the neurosurgery unit were submitted to clinical history, physical examination with sleep questionnaires, and scored on the Epworth Sleepiness Scale. Full night polysomnography was performed in an Oxford SAC system where EEG, electro-oculography, electrocardiography, chin and leg electromyography, chest and abdominal efforts, airflow, and oximetry were recorded continuously. Nine patients presented with Arnold-Chiari type I malformation, of whom six showed associated syringomyelia. The other two had basilar invagination. Ninety percent of these patients complained of sleep problems (snoring, choking, and witnessed apneas) and 72% presented hypersomnolence (ESS >9). The polysomnographic findings showed sleep fragmentation in 81% of the patients and a reduction of rapid eye movement sleep in 63%. The apnea/hypopnea index was above 5 in 72%, with a predominance of central apnea. Patients with craniocervical disorders present a higher probability of displaying sleep respiratory disturbances. Their sleep complaints should be assessed and patients should be submitted to an overnight sleep recording in order to identify sleep apnea.

Traumatic vertical atlantoaxial instability: the risk associated with skull traction. Case report and literature review.

Traumatic overdistraction between C1 and C2 may occur when all the ligaments connecting C2 to the skull are ruptured, and may be manifested when an attempt to reduce C1-C2 subluxation is made by means of traction. We describe here the case of a patient with traumatic anterior atlantoaxial dislocation, who developed atlantoaxial vertical dissociation after skull traction using a Gardner-Halo with lb 4.02 (1.5 kg) of weight. The identification of patients who are susceptible to this complication is difficult. In this case, it might have been prevented by avoiding spinal traction. The aim of this report was to show that vertical dissociation may occur in C -C2 anterior dislocation submitted to spinal traction, and that other forms of reduction must be considered to treat these pathologies and avoid this potentially fatal complication.

Carotid artery-sygmoid sinus fistula: a rare complication of gunshot wound on the base of the cranium.

Vascular lesions without clinical manifestation may occur in cranial-facial wounds produced by bullets that course the base of the cranium. This work describes a rare kind of vascular complication in cranial-facial gunshot wound. The authors present the case of a patient, the victim of a cranium-maxillary gunshot wound. Carotid angiography revealed a carotid-sygmoid sinus fistula that filled the sygmoid and transverse sinuses, concomitant to the arterial angiographic phase. A direct communication between the external carotid artery and the sygmoid sinus was disclosed. We are not aware of any other description of this vascular complication in cranial gunshot wound. It is important to recognize this kind of complication in cases of cranial-facial gunshot wound, because new factors harmful to the brain perfusion systems are introduced, in addition to the alterations to venous return and intracranial pressure, caused by the primary trauma. The new non-invasive vascular diagnostic methods are proving useful in filling the gap left by arteriography, which is no longer used in these cases.