RESUMO
Additive manufacturing currently facilitates new avenues for materials discovery that have not been fully explored. In this study we reveal how additive manufacturing can be leveraged to produce dispersion strengthened (DS), multi-principal element alloys (MPEA) without the use of traditional mechanical alloying or chemical reactions. This new processing technique employed resonant acoustic mixing to coat an equiatomic NiCoCr powder with nano-scale yttrium oxides. Then, through laser powder bed fusion (L-PBF), the coated powder was successfully consolidated into 99.9% dense parts. Microstructural analysis confirmed the successful incorporation and dispersion of nano-scale oxides throughout the build volume. Furthermore, high temperature mechanical testing of the DS alloys showed significant improvements in strength and ductility over the baseline NiCoCr. As a result, this recently discovered processing route opens a new alloy design and production path that is synergistic between additive manufacturing and dispersion strengthening, possibly enabling a new generation of high-performance alloys.
RESUMO
We examined the effects of dissolved nitric oxide (NO) gas on cytoplasmic calcium levels ([Ca(2+)](i)) in C6 glioma cells under anoxic conditions. The maximum elevation (27 +/- 3 nM) of [Ca(2+)](i) was reached at 10 microM NO. A second application of NO was ineffective if the first was >0.5 microM. The NO donor diethylamine/NO mimicked the effects of NO. Acute exposure of the cells to low calcium levels was without effect on the NO-evoked response. Thapsigargin (TG) increased [Ca(2+)](i) and was less effective if cells were pretreated with NO. Hemoglobin inhibited the effects of NO at a molar ratio of 10:1. 8-Bromo-cGMP was without effect on the NO-evoked response. If cells were pretreated with TG or exposed chronically to nominal amounts of calcium, NO decreased [Ca(2+)](i). The results suggest that C6 glioma cells have two receptors for NO. One receptor (NO(A)) elevates [Ca(2+)](i) and resides on the endoplasmic reticulum (ER). The other receptor (NO(B)) decreases [Ca(2+)](i) and resides on the plasmalemma or the ER. The latter receptor dominates when the level of calcium within intracellular stores is diminished.
Assuntos
Cálcio/metabolismo , Óxido Nítrico/farmacologia , Aerobiose , Animais , Hipóxia Celular/fisiologia , Citoplasma/metabolismo , Citosol/metabolismo , Digitonina/farmacologia , Ácido Egtázico/farmacologia , Glioma , Hemoglobinas/farmacologia , Homeostase , Hidrazinas/farmacologia , Cinética , Óxido Nítrico/fisiologia , Doadores de Óxido Nítrico/farmacologia , Óxidos de Nitrogênio , Tapsigargina/farmacologia , Células Tumorais CultivadasRESUMO
The genome of the flowering plant Arabidopsis thaliana has five chromosomes. Here we report the sequence of the largest, chromosome 1, in two contigs of around 14.2 and 14.6 megabases. The contigs extend from the telomeres to the centromeric borders, regions rich in transposons, retrotransposons and repetitive elements such as the 180-base-pair repeat. The chromosome represents 25% of the genome and contains about 6,850 open reading frames, 236 transfer RNAs (tRNAs) and 12 small nuclear RNAs. There are two clusters of tRNA genes at different places on the chromosome. One consists of 27 tRNA(Pro) genes and the other contains 27 tandem repeats of tRNA(Tyr)-tRNA(Tyr)-tRNA(Ser) genes. Chromosome 1 contains about 300 gene families with clustered duplications. There are also many repeat elements, representing 8% of the sequence.
Assuntos
Arabidopsis/genética , Genoma de Planta , Mapeamento Cromossômico , DNA de Plantas , Duplicação Gênica , Dados de Sequência Molecular , Família Multigênica , Proteínas de Plantas/genética , RNA de Transferência/genéticaRESUMO
Accurate knowledge of the internal diameter (id) of micropipet tips is important, because the ability to study many different aspects of biological membranes is a very sensitive function of tip size. The authors examined two methods used to characterize pipet tips: the digital manometric method (DMM) and bubble number method (BNM). For DMM, the authors compared the ability of Laplace's equation (model I) and a modified form of his equation (model II), which accounts for adhesion between the test fluid and glass. Pressure measurements were made with a digital manometer, and ids at the tip were measured using scanning electron microscopy (SEM). The micropipet tips showed a slight asymmetry in id, with a approx 5% difference between maximum and minimum id. On average, model I overestimates the largest id by 2%. Model II overestimates the smaller id by 2%. For micropipet tips ranging from 1.00 to 5.00 microm, the corresponding uncertainties range from 20 to 100 nm. Making the normally hydrophilic glass surface hydrophobic strongly reduced threshold pressures when tested in water, but not 100% methanol. Compared to BNM, DMM was insensitive to changes in atmospheric pressure: BNM can be corrected for changes in atmospheric pressure. Convergence angle(s) can be determined from measurements of the pressure and the axial distance of the meniscus from the tip. The accuracy and precision of digital manometry approaches that of SEM. DMM should be particularly useful in selecting micropipets for patch clamp studies of small vesicles (< 10 microm), and may enable systematic selection of micropipets for many other experiments.
Assuntos
Microeletrodos , Técnicas de Patch-Clamp/instrumentação , Pressão Atmosférica , Desenho de Equipamento , Canais Iônicos , Manometria , Microscopia Eletrônica de Varredura , Microscopia de Vídeo , Modelos Teóricos , Tensão SuperficialRESUMO
We monitored the volume of C6 glioma cells in suspension using a Coulter Counter and exposed the cells to micromolar or nanomolar levels of collagenase or clostripain. In 13 experiments, type IV collagenase (310 units ml-1; approximately 3 microM L-1) decreased the volume by 8-12%, 8 min after addition. In 13 of 21 experiments, the volume decrease was followed by a volume regulatory increase (VRI) back to control levels in the continued presence of collagenase. The shrinkage evoked by type IV collagenase was eliminated by heat-inactivation of the enzyme preparation. A highly purified collagenase (type VII) at the same concentration evoked a relatively minor decrease in volume. A well-known contaminating protease present in type IV collagenase, clostripain, which specifically cleaves arginyl peptide bonds, evoked a 7 +/- 2% shrinkage (100 nM L-1, 7 experiments). Clostripain did not evoke a volume regulatory increase. The initial velocity of shrinkage evoked by clostripain (0.0012 pL min-1, 0.0034 pL min-1, 0.0132 pL min-1; 1 pL = 10(-12) liters) scaled with its concentration (1 nM L-1, 10 nM L-1, 100 nM L-1). The effect of clostripain was inhibited by heat-inactivation of the enzyme. Leupeptin, an inhibitor of clostripain, prevented the decrease in volume evoked by clostripain. The activity of stretch-activated ion channels was unaffected by type IV collagenase. Barium, cesium, amiloride, DIDS, or bumetanide failed to block the shrinkage evoked by type IV collagenase. These results demonstrate that clostripain, present in crude collagenase enzyme preparations, causes the shrinkage, and that C6 glioma cells can undergo a volume regulatory increase at virtually constant osmotic pressure. In addition, cleavage of a cell surface moiety, which contains arginine, and possibly proline, causes shrinkage. This moiety may be part of the extracellular or intracellular matrix providing mechanical support to the cells. VRI reflect actions of another substance in the type IV crude collagenase preparations, on a receptor independent of the arg-pro moiety. The enzymatic modulation of glioma cell volume by these two receptors may reflect a new mechanism by which such cells, and possibly other glia, regulate their contact area and interactions with other cells in the central nervous system.
Assuntos
Colagenases/farmacologia , Cisteína Endopeptidases/farmacologia , Glioma/patologia , Peptídeo Hidrolases/farmacologia , Neoplasias Encefálicas/enzimologia , Tamanho Celular/efeitos dos fármacos , Inibidores de Cisteína Proteinase/farmacologia , Glioma/química , Glioma/enzimologia , Humanos , Canais Iônicos/efeitos dos fármacos , Leupeptinas/farmacologia , Metaloproteinase 9 da Matriz , Mecanorreceptores/efeitos dos fármacos , Células Tumorais Cultivadas/efeitos dos fármacosRESUMO
Arabidopsis thaliana (Arabidopsis) is unique among plant model organisms in having a small genome (130-140 Mb), excellent physical and genetic maps, and little repetitive DNA. Here we report the sequence of chromosome 2 from the Columbia ecotype in two gap-free assemblies (contigs) of 3.6 and 16 megabases (Mb). The latter represents the longest published stretch of uninterrupted DNA sequence assembled from any organism to date. Chromosome 2 represents 15% of the genome and encodes 4,037 genes, 49% of which have no predicted function. Roughly 250 tandem gene duplications were found in addition to large-scale duplications of about 0.5 and 4.5 Mb between chromosomes 2 and 1 and between chromosomes 2 and 4, respectively. Sequencing of nearly 2 Mb within the genetically defined centromere revealed a low density of recognizable genes, and a high density and diverse range of vestigial and presumably inactive mobile elements. More unexpected is what appears to be a recent insertion of a continuous stretch of 75% of the mitochondrial genome into chromosome 2.
Assuntos
Arabidopsis/genética , Mapeamento Cromossômico , DNA de Plantas , Genes de Plantas , Núcleo Celular/genética , Centrômero , Evolução Molecular , Duplicação Gênica , Genes de Plantas/fisiologia , Mitocôndrias/genética , Dados de Sequência Molecular , Proteínas de Plantas/genética , Proteínas de Plantas/fisiologia , Análise de Sequência de DNARESUMO
I used capillary rise methods to investigate the lumenal surface properties of quartz (fused silica, Amersil T-08), borosilicate (Corning 7800), and high-lead glass (Corning 0010) capillaries commonly used to make patch pipets. I calculated the capillary rise and contact angle for water and methanol from weight measurements. The capillary rise was compared with the theoretical maximum value calculated by assuming each fluid perfectly wetted the lumenal surface of the glass (i.e., zero contact angle, which reflects the absence of surface contamination). For borosilicate, high-lead, and quartz capillaries, the rise for water was substantially less than the theoretical maximum rise. Exposure of the borosilicate, lead, and quartz capillaries to several cleaning methods resulted in substantially better--but not perfect--agreement between the theoretical maximum rise and calculated capillary rise. By contrast, the capillary rise for methanol was almost identical in untreated and cleaned capillaries, but less than its theoretical maximum rise. The residual discrepancy between the observed and theoretical rise for water could not be improved on by trying a variety of cleaning procedures, but some cleaning methods were superior to others. The water solubility of the surface contaminants, deduced from the effectiveness of repeated rinsing, was different for each of the three types of capillaries examined: Corning 7800 > quartz > Corning 0010. A surface film was also detected in quatz tubing with an internal filament. I conclude that these borosilicate, quartz, and high-lead glass capillaries have a film on the lumenal surface, which can be removed using appropriate cleaning methods. The surface contaminants may be unique to each type of capillary and may also be hydrophobic. Two simple methods are presented to quantitate the cleanliness of glass capillary tubing commonly used to make pipets for studies of biological membranes. It is not known if the surface film is of importance in electrophysiological studies of biological membranes.
Assuntos
Ação Capilar , Vidro , Técnicas de Patch-Clamp/instrumentação , Detergentes , Canais Iônicos , Matemática , Metanol , Controle de Qualidade , Quartzo , Propriedades de Superfície , Tensão Superficial , Água , MolhabilidadeRESUMO
Mechanotransducing (MS) ion channels and images of the patch membrane were studied in cell-attached patches in C6 glioma cells. MS channel density was approximately 0.08 to 0.5 channels/microns2, channel conductance was approximately 40 pS (at -40 mV), and the reversal potential was +15 mV. Replacement of NaCl with KCl, CsCl, or Na gluconate in the pipette solution was without substantial effect on the current-voltage relationship. Replacement of NaCl with NMDG (N-Methyl-D-Glucamine) Cl or reducing NaCl decreased the amplitude of inward currents at negative membrane potentials and caused the reversal potential to shift in the negative direction. Rapid application of suction to the back of the pipette usually elicited a fast (< 0.1 s) appearance of channel activity. The peak (phasic) in channel activity was followed by a decrease to a constant (tonic) level of activity. The reduction in channel activity--called adaptation--was reduced at depolarizing membrane potentials and disappeared if too much pressure was applied. Positive pressure caused the patch membrane to curve toward the pipette tip, move in the direction of the tip, and evoke MS channel activity. Removal of the positive pressure caused the patch to move back to the original position. Conversely, negative pressure caused the patch membrane to curve away from the pipette tip, move away from the tip, and elicit MS channel activity. Gigohm seal resistances were always maintained during translational movement of the patch membrane. Tonic MS channel activity was not associated with translational movements of the patch membrane. Phasic and tonic channel activity were independent of the sign of curvature of the patch membrane. C6 glioma cells have rapidly adapting voltage-dependent MS ion channels, which are non-selective for monovalent cations, and belong to the stretch-activating class of mechanosensory ion channels. Adaptation in MS channels may allow the cell to limit the influx of cations in response to mechanical input. The selective loss of adaptation suggests that the MS channel's gate receives input from two sources. A minimal viscoelastic mechanical model of adaptation and two alternative models for translational movement of the patch are presented.
Assuntos
Neoplasias Encefálicas/fisiopatologia , Glioma/fisiopatologia , Canais Iônicos/fisiologia , Transdução de Sinais/fisiologia , Adaptação Fisiológica , Animais , Eletrofisiologia , Ativação do Canal Iônico/fisiologia , Potenciais da Membrana/fisiologia , Modelos Biológicos , Técnicas de Patch-Clamp , Perfusão , Pressão , Ratos , Células Tumorais CultivadasRESUMO
Peptides corresponding to the loop regions of the fourth, fifth, and sixth epidermal growth factor (EGF)-like domains of thrombomodulin (TM) have been synthesized and assayed for thrombin inhibition, as indicated by both inhibition of thrombin-mediated fibrinogen clotting and inhibition of the association of thrombin with TM that results in protein C activation. Peptides from the fifth EGF-like domain showed significant inhibition of fibrinogen clotting and protein C activation, whereas peptides from the fourth and sixth EGF-like domains were weak inhibitors in both assays. Two structural features were important for inhibitory potency of the peptides from the fifth EGF-like domain: cyclization by a disulfide bond and attachment of the "tail" amino acids C-terminal to the disulfide loop. Linear control peptides did not significantly inhibit clotting or protein C activation. The C-terminal loop alone, the "tail" peptide, or a mixture of the two were at least 10-fold less potent inhibitors of clotting or protein C activation. A more constrained peptide analog was designed by deletion of an isoleucine within the C5-C6 disulfide loop, TM52-1 + 5C. This analog was a better inhibitor in both assay systems, having a Ki for protein C activation of 26 microM.
Assuntos
Peptídeos Cíclicos/química , Peptídeos Cíclicos/farmacologia , Trombina/antagonistas & inibidores , Trombomodulina/química , Sequência de Aminoácidos , Sítios de Ligação , Coagulação Sanguínea/efeitos dos fármacos , Fator de Crescimento Epidérmico/química , Humanos , Cinética , Espectrometria de Massas , Dados de Sequência Molecular , Peso Molecular , Peptídeos Cíclicos/síntese química , Ligação Proteica , Proteína C/antagonistas & inibidores , Proteína C/metabolismo , Trombina/metabolismo , Trombomodulina/metabolismoRESUMO
Ion channels present on the soma of neonatal rat astrocytes in primary cell culture were studied using the single channel recording technique. Ion channels were activated by changing the pressure in the back of the pipette. The morphological structure of the patch membrane was examined while recording channel activity. One class of channel was activated by increasing the pipette pressure (curvature-sensitive or CS channels). CS channels were observed in 150 mM KCl, 150 mM NaCl, or 150 mM sodium gluconate. At constant pressure the closed times decreased with depolarization. CS channels had a conductance of 50 pS in 150 mM NaCl, and displayed an inwardly rectifying current-voltage relationship. CS channel activity was found only in cell-attached patches, and were active only when the patch membrane curved towards the soma. The other class of channel was found to be activated by both suction and pressure (stretch-activated or SA channels). Four SA conductance levels were found: 360, 230, 144, and 70 pS in 150 mM KCl. Each conductance displayed a linear current-voltage relationship. At negative membrane potentials SA channels were inhibited by Cs+, Ba2+ or Na+. The relationship between average mechanosensory current and pressure was biphasic for SA channels and monophasic for CS channels. Combinations of SA and CS channels could be observed in the same patch. We propose that CS channels are non-specific cation channels which sense membrane tension only when the patch membrane is in a specific, permissive curvature. SA channels appear to be K(+)-selective channels that sense membrane tension independent of the direction of curvature.
Assuntos
Astrócitos/metabolismo , Canais Iônicos/fisiologia , Transdução de Sinais/fisiologia , Animais , Células Cultivadas , Córtex Cerebral/fisiologia , Césio/farmacologia , Eletrofisiologia , Ativação do Canal Iônico , Potenciais da Membrana/fisiologia , Canais de Potássio/fisiologia , Cloreto de Potássio/farmacologia , Pressão , Ratos , Reflexo de Estiramento/fisiologiaRESUMO
The contractile protein myosin is thought to subserve motility-related functions in a wide range of eukaryotic non-muscle cells including both neurons and glia. To determine if the Ca2+/calmodulin-dependent enzyme, myosin light chain kinase (MLCK) is involved in the regulation of neural myosin we investigated the presence and localization of MLCK in a variety of neural tissues by immunoblotting and immunocytochemistry. A specific immunoreactive protein (M(r) = 146,000) was detected in blotted homogenates from many regions of rat brain and from primary cultures of either astrocytes or cerebellar granule cells grown in the absence of other cell types. At the light microscopic level, MLCK-immunoreactivity was evident in many regions of rat brain, as well as in the cultured astrocytes and cerebellar granule cells. MLCK-immunoreactivity was observed to be largely cytosolic in astrocytes but with a proportion associated with the cytoskeleton. In the cerebellar granule cells immunoreactivity was present in neuronal processes as well as somata. The detection of MLCK in neural cells suggests that MLCK-catalyzed myosin phosphorylation may couple changes in intracellular calcium concentrations to motility-related functions of neurons and glia.
Assuntos
Astrócitos/enzimologia , Quinase de Cadeia Leve de Miosina/biossíntese , Proteínas do Tecido Nervoso/biossíntese , Neurônios/enzimologia , Animais , Células Cultivadas , Imunofluorescência , Expressão Gênica , Immunoblotting , RatosRESUMO
The long-standing question concerning the direct actions of glutamate on the membrane potential of astroglial cells in the central nervous system was addressed using the in vitro kainic acid-lesioned hippocampal slice preparation and primary cell co-cultures of astrocytes and microglia derived from such lesions. The ultrastructure of the lesioned hippocampus was examined to aid in the identification of the cells appearing in culture. In culture, microglia appeared as flat cells, less than 1 micron in thickness at the edge of the cell, but thicker (about 5 microns) near the nucleus. The cytoplasm was packed with granular inclusions. Microglia appeared in two morphological forms, amoeboid and ramified. The amoeboid form was characterized by a cell body with a single process, and was always observed 1 day after starting the cell culture. Such cells became less frequent after 1 week in culture. The ramified form appeared as a rounded cell, devoid of processes, and were frequently observed in older cultures (greater than 1 week). Microglia did not round up after exposure to dibutyrylcyclic adenosine monophosphate (cAMP), and did not stain for glial fibrillary acidic protein (GFAP). An ultrastructural examination of the lesion demonstrated that microglia were present and that they contained many cytoplasmic granules similar to lipofuscin-containing granules. No filaments were observed in the cytoplasm of microglia. By contrast, the cytoplasm of astrocytes in culture had far fewer granules, rounded up to dibutyryl-cAMP, exhibited multiple processes, and stained for GFAP. In slices, astrocytes had no lipofuscin-containing granules, but numerous cytoplasmic filaments were present.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Astrócitos/fisiologia , Glutamatos/farmacologia , Hipocampo/fisiologia , Junções Intercelulares/fisiologia , Ácido Caínico/farmacologia , Neuroglia/fisiologia , Animais , Astrócitos/citologia , Astrócitos/efeitos dos fármacos , Bucladesina/farmacologia , Núcleo Celular/ultraestrutura , Células Cultivadas , Eletrofisiologia , Feminino , Ácido Glutâmico , Hipocampo/efeitos dos fármacos , Técnicas In Vitro , Junções Intercelulares/efeitos dos fármacos , Junções Intercelulares/ultraestrutura , Potenciais da Membrana/efeitos dos fármacos , Mesoderma , Neuroglia/citologia , Neuroglia/efeitos dos fármacos , Tratos Piramidais/efeitos dos fármacos , Tratos Piramidais/fisiologia , Ratos , Ratos EndogâmicosRESUMO
The membrane potentials of astrocytes in primary cultures prepared from neonatal rat cerebral cortices were depolarized by (-)-norepinephrine. The average first response to 10(-5) M (-)-norepinephrine was 24 mV from an average resting potential of -68 mV, and the average for the second response was 14 mV. Thus this process showed marked desensitization. The response was attributed to an activation of an alpha 1-receptor since it was about 1000 times more sensitive to inhibition by prazosin than to yohimbine or idazoxan. In addition, depolarization was seen to the application of 10(-5) M phenylephrine.
Assuntos
Astrócitos/fisiologia , Norepinefrina/farmacologia , Receptores Adrenérgicos alfa/fisiologia , Animais , Animais Recém-Nascidos , Astrócitos/efeitos dos fármacos , Células Cultivadas , Dioxanos/farmacologia , Idazoxano , Cinética , Potenciais da Membrana/efeitos dos fármacos , Prazosina/farmacologia , Ratos , Receptores Adrenérgicos alfa/efeitos dos fármacos , Ioimbina/farmacologiaRESUMO
Intact giant mitochondria isolated from the liver of mice fed a diet containing cuprizone were studied using patch microelectrodes. The current-voltage curves were nonlinear, suggesting the presence of voltage-sensitive channels. In the negative range of voltage, the channels appear to close with increasing magnitude of the voltage. The dependence of the conductance on voltage is similar to that of the outer membrane channels (VDAC) studied in planar bilayers. Occasionally, over a narrow range of positive potentials, the conductance also decreases as in the bilayer studies. However, more frequently the conductance increases sharply in a completely reversible manner at potentials greater than 10 to 20 mV. The increase in conductance with voltage may be interpreted as a major rearrangement of membrane components. Qualitatively comparable results were obtained using fused outer membranes isolated from Neurospora mitochondria. The behavior of VDAC is affected by treatment with succinic anhydride or the polyanion, polymethacrylate, maleate, styrene (1:2:3). We have found similar effects in the negative range of potentials in patches from giant mitochondria treated in the same fashion.
Assuntos
Membranas Intracelulares/fisiologia , Mitocôndrias Hepáticas/fisiologia , Animais , Condutividade Elétrica , Cinética , Bicamadas Lipídicas , Potenciais da Membrana , Camundongos , Camundongos EndogâmicosRESUMO
We have examined the question of anion-transport systems in glia using primary astrocyte cultures prepared from neonatal rat brains. These studies show that these cells have exchange or cotransport systems for Cl- that appear to be electrically neutral, that is, SITS-sensitive Cl-/Cl- or Cl-/HCO3- anion exchange, and furosemide- and bumetanide-sensitive Na+ + K+ + 2Cl- cotransport. These inhibitors inhibit a major component of the total 36Cl- flux and the remaining Cl- flux may be conductive; however, this conductive flux makes a small contribution to Em relative to K+, since large changes in [Cl-]o do not usually affect Em, which is predominantly a K+ diffusion potential. We have also found an alpha-receptor-mediated depolarization that is affected by imposed changes in Ecl. The alpha-receptor-mediated depolarization seen at normal [Cl-]o could be partially due to increased Cl- conductance because [Cl-]i appears to be several-fold higher than it would be if it were in equilibrium with the membrane potential.
Assuntos
Ânions/metabolismo , Astrócitos/metabolismo , Proteínas de Transporte/metabolismo , Animais , Bicarbonatos/metabolismo , Encéfalo/metabolismo , Dióxido de Carbono/metabolismo , Células Cultivadas , Cloretos/metabolismo , Concentração de Íons de Hidrogênio , Potenciais da Membrana , Proteínas de Membrana , Ratos , Simportadores de Cloreto de Sódio-PotássioRESUMO
L-glutamic acid (L-Glu) and L-aspartic acid (L-Asp) are considered to be major excitatory amino acid transmitters, causing depolarization and excitation of neurones in the mammalian central nervous system (CNS). These responses have been thought to be an exclusively neuronal property as the excitatory amino acids either did not affect the potential of electrophysiologically unresponsive glial cells, or when an effect was seen, it was attributed to changes in external [K+] (refs 5, 6). Here we report that L-Glu directly depolarizes immunocytochemically-identified astrocytes in primary culture. L- or D-Asp and kainic acid (KA) also depolarized these cells while none or minimal changes in the resting membrane potentials were found in response to N-methyl-D-aspartate, D-glutamate, taurine, L-glutamine or to the inhibitory amino acids gamma-aminobutyric acid (GABA) and glycine. We conclude that the membrane potential of astrocytes can no longer be thought of as being responsive only to K+ and that the electrophysiological effects of excitatory amino acids in situ may not be exclusively a neuronal property.
Assuntos
Aminoácidos/farmacologia , Astrócitos/fisiologia , Aminas Biogênicas/farmacologia , Encéfalo/fisiologia , Animais , Ácido Aspártico/farmacologia , Astrócitos/efeitos dos fármacos , Células Cultivadas , Proteína Glial Fibrilar Ácida/análise , Glutamatos/farmacologia , Ácido Glutâmico , Glicina/farmacologia , Ácido Caínico/farmacologia , Potenciais da Membrana/efeitos dos fármacos , Ratos , Ácido gama-Aminobutírico/farmacologiaRESUMO
The effects of two neurotoxins, veratridine and alpha-scorpion toxin II, on the resting potential (RMP) of identified astrocytes in primary cell culture were studied using standard electophysiological techniques. Veratridine caused either a series of transient depolarizations or a single sustained depolarization. alpha-Scorpion toxin (II), which alone had no effect on the RMP, increased the duration of the veratridine-induced transient depolarizations. The depolarization caused by veratridine, or veratridine plus alpha-scorpion toxin II, was reversibly inhibited by tetrodotoxin and was reversibly reduced in magnitude by reductions of the external sodium concentration. Cells that were depolarized by veratridine were shown to contain glial fibrillary acidic protein, a marker considered specific for astrocytes. The effect of long-term (10 sec) hyperpolarizing and depolarizing current pulses on the RMP were also studied using two microelectrodes, i.e., dual impalement. In the absence of veratridine, the RMP returned to its original value after termination of the hyperpolarizing current, and the current-voltage relationship was found to be linear over a wide range of membrane potentials. In the presence of veratridine, termination of the hyperpolarizing current produced a transient depolarization that was sensitive to tetrodotoxin. Depolarizing current pulses were without effect. These results show that astrocytes in primary culture have a chemically inducible Na+ channel that appears to be voltage-dependent.
Assuntos
Astrócitos/efeitos dos fármacos , Canais Iônicos/efeitos dos fármacos , Neurotoxinas/farmacologia , Venenos de Escorpião/farmacologia , Veratridina/farmacologia , Veratrina/análogos & derivados , Eletrofisiologia , Canais Iônicos/metabolismo , Potenciais da Membrana/efeitos dos fármacos , Tetrodotoxina/farmacologiaRESUMO
A new approach for the analysis of current-voltage (IV) data is presented and applied to a variety of published data collected from various systems. Our analysis of published results shows that our method of analysis can account for the observed IV data. The calculated permeability coefficients are in reasonable agreement with those calculated from ion fluxes. In those cases where two ions are assumed to carry the current, the ratios of the calculated permeability coefficients are in agreement with those ratios determined from the Goldman-Hodgkin-Katz voltage equation. In most cases, the entire IV curve can be accounted for by using our method of analysis. In several examples, only a portion of the IV curve is in agreement with the predictions. We attribute the failure to account for the IV data to reflect the failure of one or more of the assumptions used by the GHK current equation. In other cases, assuming that an additional ion carries the current, the treatment can account for the IV data. However, the identity of the extra ion cannot be established from these published data without additional studies (e.g., ionic replacement studies).
Assuntos
Permeabilidade da Membrana Celular , Potenciais da Membrana , Animais , Axônios/fisiologia , Decapodiformes , Drosophila , Eritrócitos/fisiologia , Bicamadas Lipídicas , Matemática , Fibras Nervosas Mielinizadas/fisiologia , Junção Neuromuscular/fisiologia , Membrana Nuclear/fisiologia , Plantas , UrodelosRESUMO
Two assays of the capacity of single giant mitochondria or mitoplasts to phosphorylate ATP from Pi and ADP have been developed. One depends on the placement of a single mitochondrion next to a glycerinated myofibril, by micromanipulation. With the appropriate controls, contraction of the myofibril serves as an indication of ATP synthesis. The other assay similarly requires the isolation of one single mitochondrion but the assay of ATP synthesized uses the luciferin-luciferase reaction with a conventional photometric system. With the latter, we found that either impalement with microelectrodes or electrophoretic microinjection of two dyes, Lucifer Yellow CH or pyranine , into the inner space have no effect on the phosphorylative capacity of mitochondria or mitoplasts. The electric potential across the mitochondrial membrane was monitored during the assay and found to be small and generally positive inside.