Assuntos
Proteínas da Membrana Plasmática de Transporte de Dopamina/metabolismo , Hidrocefalia/complicações , Síndrome Maligna Neuroléptica/etiologia , Adulto , Agonistas de Dopamina/efeitos adversos , Agonistas de Dopamina/uso terapêutico , Evolução Fatal , Feminino , Escala de Coma de Glasgow , Humanos , Hidrocefalia/diagnóstico por imagem , Hidrocefalia/metabolismo , Pessoa de Meia-Idade , Síndrome Maligna Neuroléptica/diagnóstico por imagem , Síndrome Maligna Neuroléptica/metabolismo , Recuperação de Função Fisiológica , Tomografia Computadorizada de Emissão de Fóton Único , Derivação VentriculoperitonealRESUMO
Adenosine is known to exert multiple functions within the eye. The aim of this report was to find out if adenosine can be produced locally in the choroid and ciliary body. Therefore, I investigated the distribution of ecto-5'-nucleotidase (5'-NT), the key enzyme for the production of extracellular adenosine. This report provides evidence that 5'-NT is expressed in the choroid and in the ciliary body (and its processes) of the rat eye, predominantly in endothelial cells. These locations of 5'-NT indicate strategically important production sites of adenosine regulating choroid and ciliary body functions (e.g., blood flow, aqueous fluid production, and immune response).
Assuntos
5'-Nucleotidase/metabolismo , Corioide/enzimologia , Corpo Ciliar/enzimologia , Adenosina/metabolismo , Animais , Corioide/citologia , Corpo Ciliar/citologia , Técnicas Imunoenzimáticas , Masculino , Ratos , Ratos WistarAssuntos
Ataxia/diagnóstico , Hemorragia Cerebral/diagnóstico , Demência/diagnóstico , Perda Auditiva/diagnóstico , Hemossiderose/diagnóstico , Acidentes por Quedas , Biomarcadores/análise , Angiografia Cerebral , Diagnóstico Diferencial , Evolução Fatal , Humanos , Imageamento por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Tomografia Computadorizada por Raios XAssuntos
Fator de Transcrição E2F1/metabolismo , Meningite Pneumocócica/metabolismo , Animais , Caspase 3/metabolismo , Fator de Transcrição E2F1/genética , Marcação In Situ das Extremidades Cortadas , Meningite Pneumocócica/genética , Camundongos , Camundongos Endogâmicos C57BL , Camundongos KnockoutRESUMO
Infarction size and infections are important determinants of stroke outcome in humans. Bacterial infections are promoted by stroke-induced immunodeficiency which in experimental stroke is mainly characterized by extensive lymphocyte apoptosis and dysfunction. Pharmacological inhibition of caspases may improve stroke outcome not only by reducing apoptotic brain damage but also by attenuating stroke-induced immunodeficiency. We investigated the effects of systemic administration of the novel, non-toxic caspase-inhibitor quinolyl-valyl-O-methylaspartyl-[-2,6-difluorophenoxy]-methyl ketone (Q-VD-OPH) on stroke-induced neuronal and lymphocyte apoptosis, susceptibility to infections, and mortality in a murine model of stroke induced by middle cerebral artery occlusion (MCAO). Q-VD-OPH reduced ischemic brain damage and stroke-induced programmed cell death in thymus and spleen, decreased susceptibility to post-stroke bacteremia, and improved survival. Therefore, Q-VD-OPH may be a promising therapeutic agent in stroke.