Negligible Effects of ß-Hydroxy-ß-Methylbutyrate Free Acid and Calcium Salt on Strength and Hypertrophic Responses to Resistance Training: A Randomized, Placebo-Controlled Study.

This study evaluated the effects of ß-hydroxy-ß-methylbutyrate free acid (HMB-FA) and calcium salt (HMB-Ca) on strength, hypertrophy, and markers of muscle damage. In this randomized, double-blind, placebo-controlled study, 44 resistance-trained men (age: 26 ± 4 years; body mass: 84.9 ± 12.0 kg) consuming ≥1.7 g·kg-1·day-1 of protein received HMB-FA (3 g/day; n = 14), HMB-Ca (3 g/day; n = 15), or placebo (PL; cornstarch, 3 g/day; n = 15) for 12 weeks, while performing a periodized resistance training program. Before and after intervention, lean body mass (measured with dual X-ray absorptiometry), maximal dynamic strength (one-repetition maximum), knee extension maximal isometric strength (maximal voluntary isometric contraction [MVIC]), cross-sectional area (measured with ultrasound), and muscle soreness were assessed. MVIC was also measured 48 hr after the first and the last training sessions. All groups increased lean body mass (main time effect: p < .0001; HMB-FA: 1.8 ± 1.8 kg; HMB-Ca: 0.8 ± 1.4 kg; PL: 0.9 ± 1.4 kg), cross-sectional area (main time effect: p < .0001; HMB-FA: 6.6 ± 3.8%; HMB-Ca: 4.7 ± 4.4%; PL: 6.9 ± 3.8%), one-repetition maximum bench press (main time effect: p < .0001; HMB-FA: 14.8 ± 8.4 kg; HMB-Ca: 11.8 ± 7.4 kg; PL: 11.2 ± 6.6 kg), MVIC (main time effect: p < .0001; HMB-FA: 34.4 ± 39.3%; HMB-Ca: 32.3 ± 27.4%; PL: 17.7 ± 20.9%) after the intervention, but no differences between groups were shown. HMB-FA group showed greater leg press strength after the intervention than HMB-Ca and PL groups (Group × Time interaction: p < .05; HMB-FA: 47.7 ± 31.2 kg; HMB-Ca: 43.8 ± 31.7 kg; PL: 30.2 ± 20.9 kg). MVIC measured 48 hr after the first and the last sessions showed no attenuation of force decline with supplementation. Muscle soreness following the first and last sessions was not different between groups. The authors concluded that neither HMB-Ca nor HMB-FA improved hypertrophy or reduced muscle damage in resistance-trained men undergoing resistance training ingesting optimal amounts of protein. HMB-FA but not HMB-Ca resulted in a statistically significant yet minor improvement on leg press one-repetition maximum.

Determinant factors of peak treadmill speed in physically active men.

BACKGROUND: The aim of the present study was to determine whether physiological factors and maximal dynamic strength are able to determine the peak treadmill speed (PTS) in physically active individuals. METHODS: One hundred and fifty physically active healthy males voluntarily visit the laboratory on three separate occasions and underwent the following activities: first visit - IPAQ (short version), anthropometric measurements, and a maximal incremental test performed for physiological variables (maximal oxygen uptake [V̇O2] and respiratory compensation point [RCP]); second visit - constant speed test for running economy (RE) measurement, and familiarization with the Maximum Dynamic Strength (1RM) Test in the leg press exercise; third visit - 1RM test. RESULTS: The stepwise multiple regression model selected four independent variables to predict PTS (RCP, V̇O2, RE, and 1RM). RCP explained 59% (P<0.001) of variance in PTS, whereas V̇O2, RE and 1RM accounted for additional 8% (P<0.001), 4% (P<0.001), and 1.4% (P=0.038), respectively. CONCLUSIONS: In conclusion, the results of the present study demonstrate that PTS, an important predictor of endurance performance, is determined by both physiological (i.e., RCP, V̇O2, and RE) and muscular (1RM) parameters in healthy active individuals. These results demonstrate that, during a physical evaluation, PTS is able to represent physiological and muscular parameters of physically active individuals. This has the advantage during aerobic fitness evaluations of not requiring expensive equipment and specialized software.

The Association of ACE Genotypes on Cardiorespiratory Variables Related to Physical Fitness in Healthy Men.

Aerobic power (VO2max), aerobic capacity (RCP), and running efficiency (RE) are important markers of aerobic fitness. However, the influence of the angiotensin converting enzyme (ACE) polymorphism on these markers has not been investigated in healthy individuals. One hundred and fifty physically active young men (age 25 ± 3 years; height 1.77 ± 0.06 m; body mass 76.6 ± 0.9 kg; VO2max 47.7 ± 5.5 ml·kg-1·min-1) visited the laboratory on two separate occasions, and performed the following tests: a) a maximal incremental treadmill test to determine VO2max and RCP, and b) two constant-speed running tests (10 km·h-1 and 12 km·h-1) to determine RE. The genotype frequency was II = 21%; ID = 52%; and DD = 27%. There was a tendency for higher VO2max with the ACE II genotype (p = 0.08) compared to DD and ID genotypes. Magnitude based inferences suggested a likely beneficial effect on VO2max with the ACE II genotype. There was no association between genotypes for other variable. These findings suggest that individuals with the ACE II genotype have a tendency towards better values in aerobic power, but not with aerobic capacity or running economy.

GEDAE-LaB: A Free Software to Calculate the Energy System Contributions during Exercise.

PURPOSE: The aim of the current study is to describe the functionality of free software developed for energy system contributions and energy expenditure calculation during exercise, namely GEDAE-LaB. METHODS: Eleven participants performed the following tests: 1) a maximal cycling incremental test to measure the ventilatory threshold and maximal oxygen uptake (V̇O2max); 2) a cycling workload constant test at moderate domain (90% ventilatory threshold); 3) a cycling workload constant test at severe domain (110% V̇O2max). Oxygen uptake and plasma lactate were measured during the tests. The contributions of the aerobic (AMET), anaerobic lactic (LAMET), and anaerobic alactic (ALMET) systems were calculated based on the oxygen uptake during exercise, the oxygen energy equivalents provided by lactate accumulation, and the fast component of excess post-exercise oxygen consumption, respectively. In order to assess the intra-investigator variation, four different investigators performed the analyses independently using GEDAE-LaB. A direct comparison with commercial software was also provided. RESULTS: All subjects completed 10 min of exercise at moderate domain, while the time to exhaustion at severe domain was 144 ± 65 s. The AMET, LAMET, and ALMET contributions during moderate domain were about 93, 2, and 5%, respectively. The AMET, LAMET, and ALMET contributions during severe domain were about 66, 21, and 13%, respectively. No statistical differences were found between the energy system contributions and energy expenditure obtained by GEDAE-LaB and commercial software for both moderate and severe domains (P > 0.05). The ICC revealed that these estimates were highly reliable among the four investigators for both moderate and severe domains (all ICC ≥ 0.94). CONCLUSION: These findings suggest that GEDAE-LaB is a free software easily comprehended by users minimally familiarized with adopted procedures for calculations of energetic profile using oxygen uptake and lactate accumulation during exercise. By providing availability of the software and its source code we hope to facilitate future related research.

The genetics of human running: ACTN3 polymorphism as an evolutionary tool improving the energy economy during locomotion.

BACKGROUND: Covering long distances was an important trait to human evolution and continues to be highlighted for health and athletic status. This ability is benefitted by a low cost of locomotion (CoL), meaning that the individuals who are able to expend less energy would be able to cover longer distances. The CoL has been shown to be influenced by distinct and even 'opposite' factors, such as physiological and muscular characteristics, which are genetically inherited. In this way, DNA alterations could be important determinants of the characteristics associated with the CoL. A polymorphism in the ACTN3 gene (R577X) has been related to physical performance, associating the X allele with endurance and the R allele with strength/power abilities. AIM: To investigate the influence of ACTN3 genotypes on the CoL. SUBJECTS AND METHODS: One hundred and fifty healthy male individuals performed two constant speed tests (at 10 and 12 km/h) to determine the CoL. RESULTS: Interestingly, the results showed that heterozygous individuals (RX genotype) presented significantly lower CoL compared to RR and XX individuals. CONCLUSIONS: It is argued that RX genotype might generate an intermediate strength-to-endurance phenotype, leading to a better phenotypic profile for energy economy during running and, consequently, for long-term locomotion.

Influence of ACTN3 R577X polymorphism on ventilatory thresholds related to endurance performance.

The purpose of this study was to verify the association between ACTN3 polymorphism and physiological parameters related to endurance performance. A total of 150 healthy male volunteers performed a maximal incremental running test to determine the speeds corresponding to ventilatory threshold (VT) and respiratory compensation point (RCP). Participants were genotyped and divided into terciles based on the analysed variables. Genotype frequencies were compared through χ(2) test between lower and higher terciles, with the lowest or highest values of each analysed variable. ACTN3 XX genotype was over-represented in higher tercile for VT and RCP. Odds ratio also showed significantly higher chances of XX individuals to be in higher tercile compared to RR (7.3) and RR + RX (3.5) for VT and compared to RR genotype (8.1) and RR + RX (3.4) for RCP. Thus, XX individuals could attain the VT and RCP at higher speeds, suggesting that they are able to sustain higher running speeds in lower exercise intensity domains. It could result in higher lipid acids oxidation, saving muscle glycogen and delaying the fatigue during prolonged exercises, which could be the advantage mechanism of this genotype to endurance performance.

Risco de fadiga prematura, percepção subjetiva de esforço e estratégia de prova durante uma corrida de 10 km / Hazard score, rating of perceived exertion and pacing strategy during a 10 km running

O objetivo do estudo foi verificar as modificações na estratégia de prova frente às alterações do risco de fadiga prematura e da percepção subjetiva de esforço (PSE) em corredores durante uma corrida de 10 km. Participaram do estudo 55 corredores com tempo nos 10 km de 41:39 ± 3:52 min:s. A estratégia de prova e a PSE foram avaliadas a cada quilômetro. O risco de fadiga prematura foi determinado pelo produto entre a PSE e a distância restante de prova e a estratégia de prova foi determinada pela curva da velocidade e distância. A ANOVA de um caminho para medidas repetidas foi utilizada para determinar as diferenças na velocidade, PSE e risco de fadiga a cada quilômetro e entre a velocidade a cada quilômetro e a velocidade média da prova. O coeficiente de correlação de Pearson foi calculado entre a PSE e o risco de fadiga prematura com a velocidade. A velocidade do primeiro quilômetro foi 8,1% maior do que a média (p ≤ 0,001). A velocidade diminuiu gradualmente ao longo da prova, ocorrendo um novo aumento no décimo quilômetro. A PSE aumentou linearmente ao longo da prova e o risco de fadiga diminuiu significantemente após o terceiro quilômetro. Houve forte correlação negativa entre a PSE e a velocidade desenvolvida durante a prova (r = -0,80; p = 0,006). Foi observada uma correlação moderada negativa entre o risco de fadiga prematura e a velocidade (r = -0,57; p = 0,04). Com isso, os achados do presente estudo sugerem que a PSE parece ter importante papel sobre os ajustes da velocidade ao longo da prova, sendo que o aumento da velocidade observado no último quilômetro pode estar associado ao baixo risco de fadiga prematura

The aim of the study was to verify the modifications on pacing strategy induced by the hazard score of premature fatigue and rated perceived exertion (RPE) in amateur runners during a simulated 10-km running. Fifty five amateur runners with the 10-km run time of 41:39 ± 3:52 min:s participated in the study. The pacing strategy and the RPE were analyzed each kilometer. The hazard score of premature fatigue was expressed as the product of RPE by the remaining distance of the running. An ANOVA one-way for repeated measures was applied to determine possible statistical differences in speed, RPE and hazard score at each kilometer or the running speed differences at each kilometer and the average running speed. Pearson's product moment correlations were calculated between RPE and hazard of premature fatigue and running speed. The speed in the first kilometer was 8.1% higher than the average speed (p ≤ 0.001). There was a progressive decrease in speed during the running with an increment near the last kilometer. The RPE increased linearly until the end of the running and the hazard score of premature fatigue significantly decreased after the third kilometer. During the running there was a strong negative correlation between speed and RPE (r = -0.80; p = 0.006). It was also observed a moderate negative correlation between speed and hazard score of premature fatigue (r = -0.57; p = 0.04). Thus, our results suggested the important role of RPE on speed adjustment during a 10-km running and the increase of running speed near the end of the race seems to be associated to the lower score of hazard of premature fatigue

Is the COL5A1 rs12722 gene polymorphism associated with running economy?

The COL5A1 rs12722 polymorphism is considered to be a novel genetic marker for endurance running performance. It has been postulated that COL5A1 rs12722 may influence the elasticity of tendons and the energetic cost of running. To date, there are no experimental data in the literature supporting the relationship between range of motion, running economy, and the COL5A1 rs12722 gene polymorphism. Therefore, the main purpose of the current study was to analyze the influence of the COL5A1rs12722 polymorphism on running economy and range of motion. One hundred and fifty (n = 150) physically active young men performed the following tests: a) a maximal incremental treadmill test, b) two constant-speed running tests (10 km · h(-1)) and 12 km · h(-1)) to determine the running economy, and c) a sit-and-reach test to determine the range of motion. All of the subjects were genotyped for the COL5A1 rs12722 single-nucleotide polymorphism. The genotype frequencies were TT = 27.9%, CT = 55.8%, and CC = 16.3%. There were no significant differences between COL5A1 genotypes for running economy measured at 10 km · h(-1) (p = 0.232) and 12 km · h(-1) (p = 0.259). Similarly, there were no significant differences between COL5A1 genotypes for range of motion (p = 0.337). These findings suggest that the previous relationship reported between COL5A1 rs12722 genotypes and running endurance performance might not be mediated by the energetic cost of running.

Pacing strategy determinants during a 10-km running time trial: contributions of perceived effort, physiological, and muscular parameters.

The purpose of this study was to identify the main determinants of the self-selected pacing strategy during a 10-km running time trial. Twenty eight male long-distance runners performed the following tests: (a) maximal incremental treadmill test, (b) economy running test, (c) maximum dynamic strength test, and (d) 10-km running time trial on an outdoor track. A stepwise multiple regression model was used to identify the contribution of rating of perceived exertion (RPE), physiological, and muscular parameters on the pacing strategy adopted by athletes. In the start phase (first 400 m), RPE accounted for 72% (p = 0.001) of the pacing variance. Peak treadmill speed (PTS) measured during a maximal incremental test explained 52% (p = 0.001) of the pacing variance during the middle phase (400-9,600 m), whereas maximal oxygen uptake and maximum dynamic strength accounted for additional 23% (p = 0.002) and 5% (p = 0.003), respectively. In the end phase (last 400 m), PTS accounted alone for 66% (p = 0.003) of the pacing variance. These data suggest that predictors of the pacing strategy during a 10-km running time trial have a transitional behavior from perceptive (start phase) to muscular and physiological factors (middle and end phases).

Determinação dos metabolismos lático e alático da capacidade anaeróbia por meio do consumo de oxigênio / Determination of lactic and alactic metabolisms of the anaerobic capacity using oxygen uptake

Tem sido sugerido que a participação do metabolismo anaeróbio alático (MAA) pode ser estimada a partir do cálculo da fase rápida do excesso do consumo de oxigênio após o exercício (EPOC RÁPIDO). Considerando que o Déficit Máximo Acumulado de Oxigênio (MAOD) representa a quantidade total de energia transferida pelos metabolismos anaeróbios, o objetivo desse estudo foi analisar se o método de subtrair o EPOC RÁPIDO do MAOD (MAOD LA-1) proporciona uma estimativa satisfatória do metabolismo anaeróbio lático (MAL). Para esse fim, o MAOD LA-1 foi comparado ao método capaz de expressar em equivalente de oxigênio a energia oriunda do acúmulo de lactato no sangue (MAOD LA-2). Nove homens adultos ativos foram submetidos a quatro sessões experimentais: 1) um teste progressivo até a exaustão em um cicloergômetro para a mensuração do consumo máximo de oxigênio (VO2max) e da potência externa correspondente ao VO2max (WVO2max); 2 e 3) seis testes de cargas constantes (3 testes por sessão) com intensidades abaixo da WVO2max; 4) um teste de carga constante com a intensidade equivalente a 110% da WVO2max. O principal achado foi que os dois métodos (MAOD LA-1 e MAOD LA-2) empregados na estimativa da contribuição do MAL no MAOD geraram valores estatisticamente similares (p > 0,05). Além disso, os valores percentuais do MAOD LA-1 (representando o MAL) e do EPOC RÁPIDO (representando o MAA) foram de aproximadamente 78% e 22%, respectivamente. Logo, os procedimentos propostos na presente investigação podem auxiliar futuros trabalhos que porventura objetivem fragmentar as contribuições dos componentes anaeróbio do MAOD.

It has been suggested that the participation of alactic anaerobic metabolism (AAM) in physical activity can be estimated by calculating the fast component of excess post-exercise oxygen consumption (EPOC FAST). Considering that maximal accumulated oxygen deficit (MAOD) represents the total amount of energy transferred by anaerobic metabolisms, this study aimed to analyze whether subtracting EPOC FAST from MAOD (MAOD LA-1) provides a satisfactory estimate of lactic anaerobic metabolism (LAM). For this purpose, MAOD LA-1 was compared with the method by which the energy derived from blood lactate accumulation (MAOD LA-2) is expressed as oxygen equivalent. Nine active adult men were submitted to four experimental sessions: 1) a maximal incremental exercise test until exhaustion on a cycle ergometer to measure the maximal oxygen consumption (VO2max) and the external power corresponding to VO2max (WVO2max); 2) and 3) six constant-workload tests (3 per session) at intensities below WVO2max; 4) one constant-workload test at an intensity corresponding to 110% of WVO2max. The main finding was that both methods (MAOD LA-1 e MAOD LA-2) that were used to estimate the contribution of LAM in MAOD resulted in similar statistical values (p > 0.05). In addition, percentage values of MAOD LA-1 (representing MAL) and EPOC FAST (representing AAM) were approximately 78 and 22%, respectively. Thus, the procedure proposed in this paper may be of interest to future studies on the.

Estratégia adotada em provas de natação estilo crawl: uma análise das distâncias de 800 e 1500m / Pacing strategy adopted in crawl swimming competitions: an analysis of the 800-m and 1500-m distances

Em competições de natação de longa distância não está bem estabelecida qual a estratégia de prova (EP) ótima. Desta forma, o objetivo desse estudo foi caracterizar as EP adotadas por atletas de nível internacional em provas de natação de 800m (feminino) e 1500m (masculino) crawl. Foram analisadas as parciais de tempo (a cada 50m) dos atletas (n = 60) participantes das semifinais e finais dos últimos três campeonatos mundiais de natação, ocorridos no período de 2006 a 2010. A análise dos dados foi realizada por meio do teste estatístico CHAID (Chi-Squared Automatic Interaction Detection), que é uma estatística χ2 (qui-quadrado) para detectar comportamento de homogeneidade entre as variáveis. Os principais resultados encontrados foram: para as duas provas analisadas, o comportamento da velocidade em função do tempo mostrou-se semelhante à estratégia parabólica (formato em U), caracterizada por um início significativamente mais rápido (P < 0,05), seguido por uma diminuição do ritmo no trecho médio da prova, quando ocorre um novo aumento significativo (P < 0,05) na velocidade no trecho final. Desta forma, tais achados indicam que este perfil de EP pode ser classificado como ótimo para as provas de natação consideradas de longa duração, visto que foi o perfil preferencialmente adotado por atletas de nível mundial.

In long distance swimming competitions, the optimal pacing strategy is not well established. The objective of this study was to characterize the pacing strategy adopted by international level athletes in 800-m (female) and 1500-m (male) crawl swimming events. We analyzed the time of each 50 m splits for semi-finalists and finalists athletes of the last three world swimming championships from 2006 to 2010. The data analysis was performed through the statistic method named CHAID (Chi-Squared Automatic Interaction Detection), which is a statistic χ2 to detect a homogeneity behavior among the variables. The main results found were: for the two events analyzed, the velocity-versus-distance curve was similar to the parabolic pacing strategy (U-shaped), characterized by a significantly faster start (p<0.05) than mean velocity for the race, followed by a gradual decline in velocity in the middle splits, when athletes increase the velocity again (p<0.05) to produce an end spurt. In conclusion, these findings indicate that this pacing strategy profile can be classified as optimal for the long distance swimming events since it was preferentially adopted by international level athletes.

Bioenergetics and neuromuscular determinants of the time to exhaustion at velocity corresponding to VO2max in recreational long-distance runners.

The purpose of this study was to investigate the main bioenergetics and neuromuscular determinants of the time to exhaustion (T(lim)) at the velocity corresponding to maximal oxygen uptake in recreational long-distance runners. Twenty runners performed the following tests on 5 different days: (a) maximal incremental treadmill test, (b) 2 submaximal tests to determine running economy and vertical stiffness, (c) exhaustive test to measured the T(lim), (d) maximum dynamic strength test, and (e) muscle power production test. Aerobic and anaerobic energy contributions during the T(lim) test were also estimated. The stepwise multiple regression method selected 3 independent variables to explain T(lim) variance. Total energy production explained 84.1% of the shared variance (p = 0.001), whereas peak oxygen uptake (V(O2)peak) measured during T(lim)and lower limb muscle power ability accounted for the additional 10% of the shared variance (p = 0.014). These data suggest that the total energy production, V(O2)peak, and lower limb muscle power ability are the main physiological and neuromuscular determinants of T(lim)in recreational long-distance runners.

É possível determinar a economia de corrida através do teste progressivo até a exaustão? / Is possible to predict running economy using maximal incremental exercise test?

O objetivo do presente estudo foi verificar a possibilidade de se estimar a economia de corrida (EC) a partir do coeficiente angular gerado pela relação VO2 vs. intensidade de testes progressivos até a exaustão (EC INCLINA). Para tanto, 16 corredores de provas de longa duração (idade 32 ± 7 anos, massa corporal 70,0 ± 6,7 kg, estatura 173,3 ± 5,0 cm, O2máx 57,9 ± 5,8 ml·kg-1·min-1) foram submetidos a um teste incremental e a dois testes de cargas constantes (12 km·h-1 e a intensidade de 90 por cento do segundo limiar ventilatório) para a mensuração da EC. Foram detectadas correlações fracas entre o EC INCLINA e a EC estabelecida a 12 km·h-1 (r = 0,49; p = 0,054) e na intensidade de 90 por cento do segundo limiar ventilatório (r = 0,55; p = 0,027). Além disso, o EC INCLINA também estava negativamente correlacionado com a concentração sanguínea de lactato (r = -0,75; p = 0,001) e a razão de troca respiratória (r = -0,80; p < 0,001) mensuradas ao final no teste progressivo. Portanto, esses achados sugerem que, embora a sua aplicação para determinar a EC seja limitada, o EC INCLINA pode ser um parâmetro alternativo empregado para o diagnóstico da aptidão de corredores de provas de longa duração devido a sua relação com o metabolismo anaeróbio.

The purpose of the present study was to investigate the association between running economy (RE) and the slope of the regression line obtained as the individual relationship between oxygen uptake and the corresponding intensity in the incremental test (EC INCLINA). Sixteen recreational long-distance runners (age 32 ± 7 years, body mass 70,0 ± 6.7 kg, height 173.3 ± 5.0 cm, VO2max 57.9 ± 5.8 ml·kg-1·min-1) performed a progressive incremental test and two submaximal workload tests (at 12 km·h-1 and 90 percent second ventilatory threshold) to determine the RE. There was significant correlation between EC INCLINA and RE measured at 12 km·h-1 (r = 0.49; p = 0.054) and at 90 percent second ventilatory threshold (r = 0.55; p = 0.027). In addition, EC INCLINA also was negatively correlated with peak blood lactate (r = -0.75; p = 0.001) and peak respiratory exchange rate (r = -0.80; p < 0.001). These findings suggest that EC INCLINA would be an alternative parameter employed to determine the endurance performance in recreational long-distance runners.