RESUMO
The authors treated 54 cases of hyperlipidemia with the antihyperlipidemic capsules and the total short-term effective rate was 77.8%. The capsules also showed obvious effects in improving hemorheology, reducing aggregation of thrombocytes, and lowering the blood pressure. The authors believe they would be useful in the prevention and treatment of ischemic apoplexy and coronary disease.
Assuntos
Medicamentos de Ervas Chinesas/uso terapêutico , Hiperlipidemias/tratamento farmacológico , Agregação Plaquetária/efeitos dos fármacos , Idoso , Viscosidade Sanguínea/efeitos dos fármacos , Cápsulas , Medicamentos de Ervas Chinesas/farmacologia , Feminino , Humanos , Hiperlipidemias/sangue , Masculino , Pessoa de Meia-Idade , Inibidores da Agregação Plaquetária , ReologiaRESUMO
Fragments (larger than 4 kb) of partially Sau 3AI-digested plasmid DNA from Bacillus thuringiensis subsp. kenyae 7404 and kurstaki HD-1 were cloned into the BamH I site of pBR322. Four transformants, containing the corresponding delta-endotoxin gene and producing proteins that reacted with antiserum against crystalline protein, were selected on the basis of results from in situ colony hybridization, radioimmunologic screening, and Western blot analysis. Three of those tested, i.e., the lysates of one transformant (TK89) carrying the delta-endotoxin gene of B.t. 7404 and two transformants (TH12 and TH48) carrying the delta-endotoxin gene of HD-1, were toxic for tobacco budworm (Heliothis assulta) and armyworm (Leucania separata Walker) caterpillars. This is the first report on cloning of the delta-endotoxin gene from B.t. subsp. kenyae, which is different in serotype from the well-studied subsp. kurstaki.
Assuntos
Bacillus thuringiensis/genética , Proteínas de Bactérias , Toxinas Bacterianas , Endotoxinas/genética , Escherichia coli/genética , Plasmídeos , Animais , Toxinas de Bacillus thuringiensis , Western Blotting , Clonagem Molecular , DNA Bacteriano/isolamento & purificação , Endotoxinas/farmacologia , Expressão Gênica , Proteínas Hemolisinas , Mariposas , Hibridização de Ácido Nucleico , Radioimunoensaio , Transformação BacterianaRESUMO
The genomic DNA of CaMV (Xinjiang isolate) was mapped on the virion DNA with a number of restriction endonucleases by double digestions and partial digestions of one-end 32P-labelled fragments. It contained the unique sites of BglI, SalI and XhoI. BamHI and HhaI each cut it at two sites, EcoRI at five sites (three of which were located), HpaII at seven sites, BglII at eight sites, and HaeIII at ten sites. The sites of all the enzymes used above, as well as HindIII which cut at ten sites were also mapped on the cloned CaMV DNA. Virion DNA and cloned viral DNA gave the same results in the number and location of cleavage sites of the restriction enzymes tested on both DNAs. Like most of the other isolates, CaMV-Xinjiang isolate has three single-stranded discontinuities. From the digestion patterns of SalI, XhoI, EcoRI and HhaI, it is concluded that the restriction map of CaMV-XJ genome is closely related to that of BKMV isolate from East Germany.