[Behavioral and physiological responses to hypoxia stress in male and female Macrobrachium rosenbergii]. / 雌雄罗氏沼虾应对低氧胁迫的行为生理响应.

To explore the physiological and behavioral responses of male and female Macrobrachium rosenbergii under hypoxia stress, an experiment with three dissolved oxygen (DO) levels (6.46, 4.48 and 3.27 mg·L-1, 6.46 mg·L-1 as control) was conducted. The enzyme activities of energy metabolism in hepatopancreas and muscles of male and female M. rosenbergii were measured after six days of hypoxia stress. The results showed that the enzyme activities of aerobic metabolism in muscles and swimming abilities were significantly decreased as DO decreased from 6.46 mg·L-1 to 4.48 mg·L-1, with the decreases being less in males than females. There was no significant difference in enzyme activities of anaerobic metabolism. When DO was further decreased to 3.27 mg·L-1, the enzyme activities of aerobic metabolism and anaerobic metabolism in muscles significantly decreased. The activity of lactate dehydrogenase (LDH) in anaerobic metabolism of hepatopancreas and tail-flipping speeds significantly decreased in males and females, with less decrease in females than that in males for LDH activity of hepatopancreas. The swimming ability was positively correlated with the enzyme activities of aerobic metabolism in pleopods muscles. There was significant correlation between tail-flipping abilities and enzyme activities of anaerobic metabolism. M. rosenbergii could reduce its reliance on energy metabolism during hypoxia stress, but with negative consequences on locomotor abilities. The muscles were preferentially powered to meet energy requirements of locomotion in males, while females gave priority to energy supply for hepatopancreas under insufficient oxygen conditions.

Simultaneous in situ visualization and quantitation of dual antigens adsorbed on adjuvants using high content analysis.

Aim: Traditional antigenicity assay requires antigen recovery from the particulate adjuvants prior to analysis. An in situ method was developed for interrogating vaccine antigens with monoclonal antibodies while being adsorbed on adjuvants. Materials & methods: The fluorescence imaging-based high content analysis was used to visualize the antigen distribution on adjuvant agglomerates and to analyze the antigenicity for adsorbed antigens. Results: Simultaneous visualization and quantitation were achieved for dual antigens in a bivalent human papillomavirus vaccine with uniquely labeled antibodies. Good agreement was observed between the in situ multiplexed assays with well-established sandwich enzyme-linked immunosorbent assays. Conclusion: The streamlined procedures and the amenability for multiplexing make the in situ antigenicity analysis a favorable choice for in vitro functional assessment of bionanoparticles as vaccine antigens.