Data on statistical experimental design to formulate amphotericin B-loaded Eudragit RL100 nanoparticles coated with hyaluronic acid for the treatment of vulvovaginal candidiasis.

Data described in this article are related to the research article entitled "Amphotericin B-loaded Eudragit RL100 nanoparticles coated with hyaluronic acid (AMP EUD nanoparticles/HA) for the treatment of vulvovaginal candidiasis" [1]. In this work, we report original data on the statistical experimental design to formulate uncoated AMP EUD nanoparticles, data on the validation of spectrophotometric method to quantify the AMP released from uncoated EUD nanoparticles and coated with HA to obtain the in vitro drug release profiles as well as the drug encapsulation efficiency. In addition, we describe original data on characterization, including diameter size, polydispersity index, zeta potential, FTIR, DSC/TG, and XRD; data on diameter of in vitro inhibition halos of Candida albicans; and on the vaginal burden of infected animals treated with uncoated AMP EUD nanoparticles and AMP EUD nanoparticles/HA. Finally, different histological sections of endocervix collected from treated and untreated animals were inserted into this manuscript.

Evaluation of cytokines in type 1 diabetes patients with and without retinopathy / Avaliação de citocinas em pacientes diabéticos tipo 1 com e sem retinopatia

ABSTRACT Introduction: Diabetes mellitus is one of the greatest public health problems in the world; the numerous complications of this disease are the focus of great concern, as they are related to high morbidity and mortality. Among the complications of diabetes, diabetic retinopathy is the most common microvascular complication. Objectives: Evaluate the association of plasma levels of inflammatory cytokines interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and interferon-gamma (IFN-γ), and anti-inflammatory cytokine interleukin-10 (IL-10) with diabetic retinopathy and the correlation of plasma levels of these cytokines together. Material and method: 125 patients with type 1 diabetes mellitus were included in this study - 55 with retinopathy and 70 without retinopathy. Cytokines IL-6, TNF-α, IFN-γ and IL-10 were determined by flow cytometry. Results and discussion: A significant association of the levels of cytokines IL-6, TNF-α, IFN-γ and IL-10 with retinopathy was not observed, possibly because measurements were performed on plasma samples, but not on vitreous humor. However, a positive correlation of cytokines among themselves (p < 0.001) was observed. Conclusion: Cytokines IL-6, TNF-α, IFN-γ and IL-10 presented a positive correlation among themselves, what suggests that they may act together in the development and progression of vascular complications in diabetes patients.

RESUMO Introdução: O diabetes mellitus é considerado um dos maiores problemas de saúde pública no mundo; as numerosas complicações decorrentes dessa patologia são foco de grande preocupação, pois estão relacionadas com elevadas morbidade e mortalidade. Entre as complicações decorrentes do diabetes, a retinopatia diabética é considerada a complicação microvascular mais comum. Objetivos: Avaliar a associação dos níveis plasmáticos das citocinas inflamatórias interleucina 6 (IL-6), fator de necrose tumoral alfa (TNF-α) e interferon gama (IFN-γ) e da citocina anti-inflamatória interleucina 10 (IL-10) com a retinopatia diabética, bem como a correlação dos níveis plasmáticos dessas citocinas entre si. Material e método: Foram incluídos no estudo 125 pacientes diabéticos tipo 1, sendo 55 portadores de retinopatia e 70 sem retinopatia. As citocinas IL-6, TNF-α, IFN-γ e IL-10 foram determinadas pelo método de citometria de fluxo. Resultados e discussão: Não foi observada associação significativa dos níveis das citocinas IL-6, TNF-α, IFN-γ e IL-10 com a retinopatia, possivelmente devido às dosagens terem sido realizadas em amostras de plasma e não de humor vítreo/aquoso. Porém, foi observada correlação positiva das citocinas entre si (p < 0,001). Conclusão: As citocinas IL-6, TNF-α, IFN-γ e IL-10 apresentaram correlação positiva entre si, o que sugere que elas podem atuar em conjunto no desenvolvimento e na progressão das complicações vasculares nos pacientes diabéticos.