Physical characterization of biphasic bioceramic materials with different granulation sizes and their influence on bone repair and inflammation in rat calvaria.

Biphasic calcium phosphate bioceramics (BCP) consist of a mixture of hydroxyapatite (HA) and beta-tricalcium phosphate (ß-TCP) within the same particle. Due to their osteoconductive properties, biocompatibility and resemblance to natural bone, these materials have become a promising and suitable alternative to autologous bone grafting. First, the topography characteristics, specific surface area, and total pore volume of BCP were evaluated using scanning electron microscopy and the BET and BJH methods. Next, this study aimed to evaluate the intensity of the inflammatory process and the bone neoformation capacity of various particle sizes of BCP in the repair of critical defects in the calvaria of rats. A xenogeneic biomaterial was used in the control group. After 30, 60, and 90 days, the animals were euthanized, followed by the processing of the samples to measure the intensity of inflammatory infiltrates and the areas of bone neoformation. Our results indicate that no considerable differences were observed in the inflammatory scores in sites treated with distinct BCP grain sizes. A greater area of bone neoformation was measured in the xenogeneic group at all analysis times, with no substantial differences in bone formation between the BCP particle size in the range of 250-500 µm and 500-1000 µm.

Nova técnica L-PRF segmentada para procedimentos regenerativos e implantares / A new L-PRF segmented technique for regenerative and dental implant procedures

Este trabalho teve o objetivo de relatar o caso clínico de paciente com rebordo em lâmina de faca na região anterior (incisivo lateral), tratado com a combinação de enxerto autógeno particulado associado a enxerto alógeno derivado de osso bovino inorgânico. Membranas de L-PRF e malha de titânio foram utilizadas para proteger e estabilizar o enxerto. O paciente teve o implante instalado na região seis meses após a realização do enxerto ósseo. No caso clínico apresentado, foram associadas as vantagens da L-PRF como matriz de suporte para as proteínas morfogenéticas ósseas (BMPs) na região em contato com o rebordo reabsorvido, combinadas ao osso particulado autógeno e ao osso bovino inorgânico. Também foram utilizadas membranas de L-PRF sobre a malha de titânio, no intuito de servirem como curativos de fibrina, contribuindo para acelerar e melhorar o processo de cicatrização da ferida cirúrgica. O uso da L-PRF para a resolução deste caso clínico indicou melhora e aceleração da cicatrização da ferida cirúrgica, maior espessura gengival e auxílio na maturação do enxerto ósseo, o que contribuiu para o resultado estético final dos tecidos peri-implantares.

This study aims to report a case of a patient with a “knife-edged” ridge in the anterior region (lateral incisor) that was treated with a combined graft of particulated autogenous bone and anorganic bovine mineral bone. L-PRF membranes and a titanium mesh were used to protect and stabilize the graft. The patient had a threaded implant installed six months after the grafting procedures. This clinical report associated the advantages of L-PRF as a matrix scaffold for bone morphogenetic proteins (BMP) in the graft region in contact with the bone ridge and combined with particulated autogenous bone and anorganic bovine bone-derived mineral grafts. L-PRF membranes were also used as fibrin bandages, aiming to improve the healing process. The use of L-PRF in the present clinical report suggested an improved graft healing, increased gingival width and aided the bone maturing process, which finally contributed to the esthetic outcome of the peri-implant tissues.