In vitro study on antioxidant and lipid-lowering activities of tobacco polysaccharides.

Tobacco polysaccharides were extracted by hot water extraction, and purified and separated using DEAE-52 cellulose chromatography columns, and three purified polysaccharide fractions, YCT-1, YCT-2, and YCT-3, were finally obtained. The physicochemical properties of the three fractions were analyzed by ultraviolet spectroscopy, high-performance liquid chromatography and high-performance gel chromatography. The in vitro antioxidant activity of tobacco polysaccharides was compared among different fractions by using DPPH radical, hydroxyl radical scavenging assay and potassium ferricyanide method. The in vitro hypoglycemic activity was compared using α-amylase and α-glucosidase activity inhibition assay. And the in vitro hypolipidemic activity were investigated by using pancreatic lipase activity inhibition assay and HepG-2 intracellular lipid accumulation assay. All the results showed that the constituent monosaccharides of the three tobacco polysaccharide fractions were similar, but the molar percentages of each monosaccharide were different. The average molecular weights of the three components were 27,727 Da, 27,587 Da, and 66,517 Da, respectively, and the scavenging activities on DPPH radicals and hydroxyl radicals were at a high level with good quantitative-effect relationships. The reducing power were much lower than that of the positive control VC, and the three polysaccharide fractions had a weak inhibitory ability on α-amylase activity, but showed excellent inhibitory ability on α-glucosidase and pancreatic lipase activity. In addition, the results of cellular experiments showed that all three fractions were able to inhibit lipid over-accumulation in HepG-2 cells by increasing the mRNA expression levels of PPAR-α, CPT-1A, and CYP7A1 genes, and the tobacco polysaccharide YCT-3 showed the best effect. The mechanism by which YCT-3 ameliorated the over-accumulation of intracellular lipids in HepG-2 cells was found to be related to its influence on the expression of miR-155-3p and miR-17-3p in the exosomes of HepG-2 cells.

Novel pretreatment with hydrogen peroxide enhanced microwave biochar for heavy metals adsorption: Characterization and adsorption performance.

Hydrogen peroxide (HP) was used to pretreat wheat straw (WS) for microwave biochar production at 100-600 W, the physicochemical properties of pretreated WS and biochar products as well as heavy metals adsorption performance were investigated. Results showed that HP enhanced specific surface area (SSA) and pore volume (PV) of WS, and the largest SSA (190.35 m2 g-1) and PV (0.1493 cm3 g-1) of biochar were obtained at microwave powers of 600 W (HPWS600) and 500 W (HPWS500), respectively. HPWS500 showed maximum adsorption capacities, which were 57.56, 190.21, and 65.16 mg g-1 for Cd2+, Pb2+, and Cu2+, respectively. Solution pH values and cation concentrations exhibited significant effects on adsorption capacities of biochar. The pseudo-second-order kinetic and Langmuir isotherm models fitted better for metal adsorption process. The FTIR results suggested that chemisorption mechanisms including precipitation with carbonate and complexation with oxygen-containing functional groups might be predominant adsorption mechanisms. These results suggest that HP pretreatment has excellent potential for biochar production.

Microwave biochar produced with activated carbon catalyst: Characterization and adsorption of heavy metals.

Novel microwave biochar derived from wheat straw (WS) using a range of power levels, with activated carbon catalyst as microwave absorber, was produced, characterized and tested as adsorbent of three heavy metals (Pb2+, Cd2+, and Cu2+). The microwave biochar with the greatest specific surface area (156.09 m2 g-1) and total pore volume (0.0790 cm3 g-1) were produced at 600 W (WS600) and 500 W (WS500) power level, respectively. Maximum adsorption capacities of WS500 to Pb2+, Cd2+ and Cu2+ were 139.44 mg g-1, 52.92 mg g-1, and 31.25 mg g-1, respectively. Optimal pH value for heavy metal removal was at range of 5-6, and Pb2+ showed the strongest affinity in competitive adsorption experiments. The adsorption data were fitted better by pseudo-second-order model and Langmuir isotherm, indicating that adsorption process was mainly explained by monolayer adsorption, and chemical adsorption occupied important role. The predominant adsorption mechanisms of heavy metals on microwave pyrolysis biochar included complexation with oxygen-containing functional groups (i.e., carboxylic acid CO and -OH) and precipitation with carbonate. In addition, reused WS600 maintained 76.17% and 96.07% of their initial adsorption capacity for Cu2+ and Cd2+, respectively. These results suggest that microwave biochar produced with activated carbon catalyst has excellent potential for efficient use in the removal of heavy metals from waste water.

Sodium oligomannate therapeutically remodels gut microbiota and suppresses gut bacterial amino acids-shaped neuroinflammation to inhibit Alzheimer's disease progression.

Recently, increasing evidence has suggested the association between gut dysbiosis and Alzheimer's disease (AD) progression, yet the role of gut microbiota in AD pathogenesis remains obscure. Herein, we provide a potential mechanistic link between gut microbiota dysbiosis and neuroinflammation in AD progression. Using AD mouse models, we discovered that, during AD progression, the alteration of gut microbiota composition leads to the peripheral accumulation of phenylalanine and isoleucine, which stimulates the differentiation and proliferation of pro-inflammatory T helper 1 (Th1) cells. The brain-infiltrated peripheral Th1 immune cells are associated with the M1 microglia activation, contributing to AD-associated neuroinflammation. Importantly, the elevation of phenylalanine and isoleucine concentrations and the increase of Th1 cell frequency in the blood were also observed in two small independent cohorts of patients with mild cognitive impairment (MCI) due to AD. Furthermore, GV-971, a sodium oligomannate that has demonstrated solid and consistent cognition improvement in a phase 3 clinical trial in China, suppresses gut dysbiosis and the associated phenylalanine/isoleucine accumulation, harnesses neuroinflammation and reverses the cognition impairment. Together, our findings highlight the role of gut dysbiosis-promoted neuroinflammation in AD progression and suggest a novel strategy for AD therapy by remodelling the gut microbiota.