RESUMO
CONTEXT: Ameloblastoma is the most common odontogenic tumor, however, the molecular pathology, especially the role of the tumor stroma, is still unclear. AIMS: To investigate the effects of the ameloblatoma-associated fibroblast (AAFs) on the proliferation and invasion of tumor cells. SETTINGS AND DESIGN: Cell culture, ELISA, proliferation and invasion assays. METHODS AND MATERIAL: The cocultivations and three-dimensional organotypic cultures of the AAFs and the tumor cells were performed. The gingival fibroblasts (GFs) were used as the control. The levels of TGF-ß and HGF in the conditioned media were analyzed using ELISA technique. The MTT proliferation assays and Boyden chamber chemoinvasion assays were also performed. STATISTICAL ANALYSIS USED: ANOVA. RESULTS: Both AAFs and GFs stimulated tumor cell growth. The TGF-ß level in the AAF group was more than those of GF group, whereas the HGF levels were not different. The AAF conditioned media also stimulated tumor cell proliferation and invasion more than the GF conditioned media. However, no difference in the thickness and morphology between the AAF and GF groups could be demonstrated in the organotypic models. CONCLUSIONS: Both AAFs and GFs support the proliferation of the tumor cells in cocultivation experiment and three-dimensional organotypic cultures. However, the AAFs have a tendency to stimulate the proliferation and induce the invasion more than GFs. Increased TGF-ß levels in the AAF condition media suggested the possible role of this growth factor in the ameloblastoma biology.
Assuntos
Ameloblastoma/metabolismo , Fibroblastos/metabolismo , Neoplasias de Cabeça e Pescoço/patologia , Linhagem Celular Tumoral , Proliferação de Células , Técnicas de Cocultura , Meios de Cultivo Condicionados , Fator de Crescimento de Hepatócito/metabolismo , Humanos , Invasividade Neoplásica , Fator de Crescimento Transformador beta/metabolismo , Células Tumorais CultivadasRESUMO
Chart recording and coding are the important processes in the healthcare informatics system, but there were only a few reports in the dentistry field. The objectives of this study are to study the qualities of dental chart recording and coding, as well as the achievement of lecture/workshop on this topic. The study was performed by auditing the patient's charts at the TU Dental Student Clinic from July 2011-August 2012. The chart recording mean scores ranged from 51.0-55.7%, whereas the errors in the coding process were presented in the coder part more than the doctor part. The lecture/workshop could improve the scores only in some topics.