Eye-Readable and Wearable Colorimetric Sensor Arrays for In Situ Monitoring of Volatile Organic Compounds.

Wearable sensors utilize changes in color as a response to physiological stimuli, making them easily recognizable by the naked eye. These colorimetric wearable sensors offer benefits such as easy readability, rapid responsiveness, cost-effectiveness, and straightforward manufacturing techniques. However, their applications in detecting volatile organic compounds (VOCs) in situ have been limited due to the low concentration of complex VOCs and complicated external interferences. Aiming to address these challenges, we introduced readable and wearable colorimetric sensing arrays with a microchannel structure and highly gas-sensitive materials for in situ detection of complex VOCs. The highly gas-sensitive materials were designed by loading gas-sensitive dyes into the porous metal-organic frameworks and further depositing the composites on the electrospun nanofiber membrane. The colorimetric sensor arrays were fabricated using various gas-sensitive composites, including eight dye/MOF composites that respond to various VOCs and two Pd2+/dye/MOF composites that respond to ethylene. This enables the specific recognition of multiple characteristic VOCs. A microfluidic channel made of polydimethylsiloxane (PDMS) was integrated with different colorimetric elements to create a wearable sensor array. It was attached to the surface of fruits to collect and monitor VOCs using the DenseNet classification method. As a proof of concept, we demonstrated the feasibility of the wearable sensing system in monitoring the ripening process of fruits by continuously measuring the VOC emissions from the skin of the fruit.

Extrusion Printing of Surface-Functionalized Metal-Organic Framework Inks for a High-Performance Wearable Volatile Organic Compound Sensor.

Wearable sensors hold immense potential for real-time and non-destructive sensing of volatile organic compounds (VOCs), requiring both efficient sensing performance and robust mechanical properties. However, conventional colorimetric sensor arrays, acting as artificial olfactory systems for highly selective VOC profiling, often fail to meet these requirements simultaneously. Here, a high-performance wearable sensor array for VOC visual detection is proposed by extrusion printing of hybrid inks containing surface-functionalized sensing materials. Surface-modified hydrophobic polydimethylsiloxane (PDMS) improves the humidity resistance and VOC sensitivity of PDMS-coated dye/metal-organic frameworks (MOFs) composites. It also enhances their dispersion within liquid PDMS matrix, thereby promoting the hybrid liquid as high-quality extrusion-printing inks. The inks enable direct and precise printing on diverse substrates, forming a uniform and high particle-loading (70 wt%) film. The printed film on a flexible PDMS substrate demonstrates satisfactory flexibility and stretchability while retaining excellent sensing performance from dye/MOFs@PDMS particles. Further, the printed sensor array exhibits enhanced sensitivity to sub-ppm VOC levels, remarkable resistance to high relative humidity (RH) of 90%, and the differentiation ability for eight distinct VOCs. Finally, the wearable sensor proves practical by in situ monitoring of wheat scab-related VOC biomarkers. This study presents a versatile strategy for designing effective wearable gas sensors with widespread applications.

A novel simple laser guidance puncture system for intracerebral hematoma.

OBJECTIVE: Accurate localization and real-time guidance technologies for cerebral hematomas are essential for minimally invasive procedures, including minimally invasive hematoma puncture and drainage, as well as neuroendoscopic-assisted hematoma removal. This study aims to evaluate the precision and safety of a self-developed laser-guided device in localizing and guiding hematoma punctures in minimally invasive surgery for intracerebral hemorrhage (ICH). METHODS: We present the components of the device and its operational procedures. Subsequently, surgeons with different titles conduct hematoma puncture experiments using the device on skull models, comparing it to freehand puncture methods and recording the offset distance from the puncture needle tip to the hematoma center. Additionally, we report the application of this device in 10 patients with ICH, assessing its accuracy and safety in comparison with a neuro-navigation system. RESULTS: In simulated puncture experiments, the accuracy of the laser-guided group surpasses that of the freehand puncture group, with a significant statistical difference observed between the two groups (P < 0.05). In the laser-guided group, there is no statistically significant difference in puncture accuracy among the surgeons (P > 0.05). In clinical experiments, no relevant surgical complications were observed. The offset distance for the laser-guided group was 0.61 ± 0.18 cm, while the neuro-navigation group was 0.48 ± 0.13 cm. There was no statistically significant difference between the two groups in terms of offset distance (P > 0.05). However, there was a significant difference in surgical duration (P < 0.05), with the former being 35.0 ± 10.5 minutes and the latter being 63.8 ± 10.5 minutes. CONCLUSION: The current study describes satisfactory results from both simulated experiments and clinical applications, achieved through the use of a novel laser-guided hematoma puncture device. Furthermore, owing to its portability, affordability, and simplicity, it holds significant importance in advancing surgical interventions for ICH, especially in underdeveloped regions.

Multimodal image fusion-assisted endoscopic evacuation of spontaneous intracerebral hemorrhage.

PURPOSE: Although traditional craniotomy (TC) surgery has failed to show benefits for the functional outcome of intracerebral hemorrhage (ICH). However, a minimally invasive hematoma removal plan to avoid white matter fiber damage may be a safer and more feasible surgical approach, which may improve the prognosis of ICH. We conducted a historical cohort study on the use of multimodal image fusion-assisted neuroendoscopic surgery (MINS) for the treatment of ICH, and compared its safety and effectiveness with traditional methods. METHODS: This is a historical cohort study involving 241 patients with cerebral hemorrhage. Divided into MINS group and TC group based on surgical methods. Multimodal images (CT skull, CT angiography, and white matter fiber of MRI diffusion-tensor imaging) were fused into 3 dimensional images for preoperative planning and intraoperative guidance of endoscopic hematoma removal in the MINS group. Clinical features, operative efficiency, perioperative complications, and prognoses between 2 groups were compared. Normally distributed data were analyzed using t-test of 2 independent samples, Non-normally distributed data were compared using the Kruskal-Wallis test. Meanwhile categorical data were analyzed via the Chi-square test or Fisher's exact test. All statistical tests were two-sided, and p < 0.05 was considered statistically significant. RESULTS: A total of 42 patients with ICH were enrolled, who underwent TC surgery or MINS. Patients who underwent MINS had shorter operative time (p < 0.001), less blood loss (p < 0.001), better hematoma evacuation (p = 0.003), and a shorter stay in the intensive care unit (p = 0.002) than patients who underwent TC. Based on clinical characteristics and analysis of perioperative complications, there is no significant difference between the 2 surgical methods. Modified Rankin scale scores at 180 days were better in the MINS than in the TC group (p = 0.014). CONCLUSIONS: Compared with TC for the treatment of ICH, MINS is safer and more efficient in cleaning ICH, which improved the prognosis of the patients. In the future, a larger sample size clinical trial will be needed to evaluate its efficacy.

Integrated Fruit Ripeness Assessment System Based on an Artificial Olfactory Sensor and Deep Learning.

Artificial scent screening systems, inspired by the mammalian olfactory system, hold promise for fruit ripeness detection, but their commercialization is limited by low sensitivity or pattern recognition inaccuracy. This study presents a portable fruit ripeness prediction system based on colorimetric sensing combinatorics and deep convolutional neural networks (DCNN) to accurately identify fruit ripeness. Using the gas chromatography-mass spectrometry (GC-MS) method, the study discerned the distinctive gases emitted by mango, peach, and banana across various ripening stages. The colorimetric sensing combinatorics utilized 25 dyes sensitive to fruit volatile gases, generating a distinct scent fingerprint through cross-reactivity to diverse concentrations and varieties of gases. The unique scent fingerprints can be identified using DCNN. After capturing colorimetric sensor image data, the densely connected convolutional network (DenseNet) was employed, achieving an impressive accuracy rate of 97.39% on the validation set and 82.20% on the test set in assessing fruit ripeness. This fruit ripeness prediction system, coupled with a DCNN, successfully addresses the issues of complex pattern recognition and low identification accuracy. Overall, this innovative tool exhibits high accuracy, non-destructiveness, practical applicability, convenience, and low cost, making it worth considering and developing for fruit ripeness detection.

Application Research of Visible Near-Infrared Spectroscopy Technology for Detecting Intracerebral Hematoma.

OBJECTIVE: To explore the visible near-infrared spectroscopic (VNIRS) characteristics of intracerebral hematoma, and provide experimental basis for hematoma localization and residual detection in hypertensive intracerebral hemorrhage (HICH) surgery. METHODS: Using VNIRS, spectral data of cerebral hematoma and cortex were collected during HICH craniotomy, and characteristic spectra were matched with paired-sample T-test. A partial least squares (PLS) quantitative model for cerebral hematoma spectra was established. RESULTS: The reflectance of cerebral hematoma spectra in the 500-800 nm band was lower than that of the cortex, and there were statistically significant differences in the 510, 565, and 630 nm bands (P < 0.05). The calibration correlation coefficient of the PLS quantitative model for cerebral hematoma spectra was R2 = 0.988, the cross-validation correlation coefficient was R2cv = 0.982, the root mean square error of calibration was RMSEC = 0.101, the root mean square error of cross-validation was RMSEV = 0.122, the external validation correlation coefficient was CORRELATION = 0.902, and the root mean square error of prediction was RMSEP = 0.426, indicating that the model had high fitting degree and good predictive ability. CONCLUSIONS: VNIRS as a noninvasive, real-time and portable analysis technology, can be used for real-time detection of hematoma during HICH surgery, and provide reliable basis for hematoma localization and residual detection.

M-Type Phospholipase A2 Receptor Autoantibody Measured by ELISA has Diagnostic Value in Patients with Idiopathic Membranous Nephropathy.

BACKGROUND: This study investigated the diagnosis value of phospholipase A2 receptor autoantibodies (PLA2R-AB) for idiopathic membranous nephropathy (IMN). METHODS: Patients with IMN, lupus nephritis, hepatitis B virus-associated nephropathy and IgA nephropathy and healthy participants were included. Receiver operating characteristics (ROC) curve was plotted for PLA2R-AB to diagnose IMN. RESULT: The level of serum PLA2R-AB was significantly higher in patients with IMN than with other MN, and was positively correlated to urine albumin-creatinine ratio and proteinuria in patients with IMN. The area under the ROC curve displaying the performance of PLA2R-AB to diagnose IMN was 0.907 with a sensitivity and specificity of 94.3% and 82.1%, respectively. CONCLUSIONS: PLA2R-AB is a reliable biomarker to diagnose Chinese patients with IMN.

Humidity-Independent Artificial Olfactory Array Enabled by Hydrophobic Core-Shell Dye/MOFs@COFs Composites for Plant Disease Diagnosis.

As a class of important artificial olfactory system, the colorimetric sensor array possesses great potential for commercialization due to its cost-effectiveness and portability. However, when applied to practical applications, the humidity interference from ambient environment and dissatisfactory sensitivity for trace target VOCs are largely unsolved problems. To overcome the problems, we developed a series of dye/MOFs@COFs gas-sensing materials with core-shell structure using a hydrophobization strategy by encapsulation of dye/metal-organic frameworks (MOFs) into hydrophobic covalent organic frameworks (COFs). Benefiting from the hydrophobic property of the COF shell, the dye/MOFs@COFs composites were endowed with excellent humidity-resistance even under 100% relative humidity (RH). Moreover, due to the uniform distribution of dyes on the porous MOFs, the dye/MOFs@COFs sensors also exhibited improved sensitivity at the sub-ppm level, compared with conventional dye sensors. On basis of the excellent humidity-resistance and improved sensitivity, an artificial olfactory array based on dye/MOFs@COFs composites was proven to be a successful practical application in early and accurate detection of wheat scab (1 day after inoculation) by monitoring its released VOC markers. The synthetic strategy for core-shell dye/MOFs@COFs is applicable to a wide range of colorimetric sensor arrays, endowing them with excellent humidity-resistance and sensitivity for the feasibility of practical applications.

Children and Adolescents' Psychological Well-Being Became Worse in Heavily Hit Chinese Provinces during the COVID-19 Epidemic.

In light of the novel coronavirus's (COVID-19's) threat to public health worldwide, we sought to elucidate COVID-19's impacts on the mental health of children and adolescents in China. Through online self-report questionnaires, we aimed to discover the psychological effects of the pandemic and its associated risk factors for developing mental health symptoms in young people. We disseminated a mental health survey through online social media, WeChat, and QQ in the five Chinese provinces with the most confirmed cases of COVID-19 during the late stage of the country-wide lockdown. We used a self-made questionnaire that queried children and adolescents aged 6 to 18 on demographic information, psychological status, and other lifestyle and COVID-related variables. A total of 17,740 children and adolescents with valid survey data participated in the study. 10,022 (56.5%), 11,611 (65.5%), 10,697 (60.3%), 6868 (38.7%), and 6225 (35.1%) participants presented, respectively, more depressive, anxious, compulsive, inattentive, and sleep-related problems compared to before the outbreak of COVID-19. High school students reported a greater change in depression and anxiety than did middle school and primary school students. Despite the fact that very few children (0.1%) or their family members (0.1%) contracted the virus in this study, the psychological impact of the pandemic was clearly profound. Fathers' anxiety appeared to have the strongest influence on a children's psychological symptoms, explaining about 33% of variation in the child's overall symptoms. Other factors only explained less than 2% of the variance in symptoms once parents' anxiety was accounted for. The spread of COVID-19 significantly influenced the psychological state of children and adolescents in participants' view. It is clear that children and adolescents, particularly older adolescents, need mental health support during the pandemic. The risk factors we uncovered suggest that reducing fathers' anxiety is particularly critical to addressing young people's mental health disorders in this time.

Shear Exfoliated Metal-Organic Framework Nanosheet-Enabled Flexible Sensor for Real-Time Monitoring of Superoxide Anion.

Recently, two-dimensional metal-organic framework (2D MOF) nanosheets have drawn a lot of attention on account of their various advantages, like ultrathin thickness, ultralarge specific surface area, abundant accessible active sites, favorable solution dispersion properties, and ease of designability. However, until now, it is still difficult to produce 2D MOF nanosheets in large scale, which hinders the practical applications of 2D MOF nanosheets. Here, for the first time, we introduced a novel shear exfoliation method to prepare scalable 2D MOF nanosheets by using a commercial blender. As a proof of concept, we used two kinds of layer-structured MOFs (ELM-12, Cu(bpy)2(OTf)2, bpy = 4,4-bipyridine, OTf = trifluoromethanesulfonate and Zn2(bim)4, bim = benzimidazole) as samples to prepare MOF nanosheets. The thickness of the two kinds of MOF nanosheets obtained is 3-5 nm. Notably, the exfoliated MOF nanosheet (ELM-12) shows improved electrochemical catalytic activity compared with its bulk counterpart. Based on this, an ELM-12 nanosheet-based flexible sensor was developed for detecting superoxide anions (O2•-) released from cancer cells. The fabricated flexible sensor displays excellent sensitivity, selectivity, flexibility, stability, and reproducibility.

Laser-induced noble metal nanoparticle-graphene composites enabled flexible biosensor for pathogen detection.

Noble metal nanoparticle-3D graphene hybrid nanocomposites possess the advantage of nanoparticles and graphene, which have attracted extensive interest. Here we developed a one-step laser induction method to prepare various noble metal nanoparticle-3D graphene nanocomposites. The nanocomposites were converted from polyimide film coated with the corresponding metal precursor-chitosan hydrogel ink. These nanoparticles including gold nanoparticles (AuNPs), silver nanoparticles (AgNPs), and platinum nanoparticles (PtNPs), were evenly distributed on the surface of porous 3D graphene. Furthermore, we prepared an AuNPs-3D graphene interdigitated array electrode using the one-step laser induction method, which was used to fabricate a flexible impedimetric immunosensor for the detection of Escherichia coli O157:H7. The immunosensor shows excellent performance including low detection limit, high selectivity, and great flexibility.

Endoscopic surgery for thalamic hemorrhage breaking into ventricles: Comparison of endoscopic surgery, minimally invasive hematoma puncture, and external ventricular drainage.

PURPOSE: Thalamic hemorrhage breaking into ventricles (THBIV) is a devastating disease with high morbidity and mortality rates. Endoscopic surgery (ES) may improve outcomes, although there is no consensus on its superiority. We investigated the efficacy and safety of ES and compared the outcomes of different management strategies by ES, hematoma puncture and drainage (HPD), and external ventricular drainage (EVD) in patients with THBIV. METHODS: We retrospectively analyzed patients with THBIV treated by ES, HPD, or EVD at our hospital from June 2015 to June 2018. Patients were categorized into anteromedial and posterolateral groups based on THBIV location, and then the two groups were further divided into ES, HPD, and EVD subgroups. Individualized surgical approach was adopted according to the location of the hematoma in the ES subgroups. Patient characteristics and surgical outcomes were investigated. RESULTS: We analyzed 211 consecutive patients. There were no significant differences in clinical characteristics or incidence of perioperative procedure-related complications (postoperative rebleeding and intracranial infection) in either anteromedial or posterolateral groups. Compared with other therapeutic methods, the ES subgroups had the highest hematoma evacuation rate, shortest drainage time, and lowest incidence of chronic ventricular dilatation (all p < 0.05). Among the three anteromedial subgroups, ES subgroup had the best clinical outcomes which was assessed by the modified Rankin Scale, followed by HPD and EVD subgroups (p < 0.01); while in the posterolateral subgroups, clinical outcomes in the ES and HPD subgroups were similar and better than that in the EVD subgroup (p = 0.037). CONCLUSION: Individualized surgical ES approach for removal of thalamic and ventricular hematomas is a minimally invasive, safe, and effective strategy for the treatment of THBIV with a thalamic hematoma volume of 10-30 mL.

Oligonucleotides and pesticide regulated peroxidase catalytic activity of hemin for colorimetric detection of isocarbophos in vegetables by naked eyes.

A novel colorimetric sensing platform based on the peroxidase activity of hemin regulated by oligonucleotide and pesticide was reported for the ultrasensitive and selective detection of isocarbophos. Oligonucleotides can accumulate on the surface of hemin in acid condition and temporarily inhibit its catalytic activity, which results in the loss of one electron of TMB molecule and produce the blue products. With the addition of isocarbophos, the pesticide molecules can interact with oligonucleotides to form some complexes, which relieve the inhibition of ssDNA to hemin and further enhance its catalytic activity. Thus, the TMB molecules are further oxidized to lose another electron and produce the yellow product in a few minutes, which has the characteristic absorption peak at 450 nm. The color change of the sensing system is related to the amount of isocarbophos, so this method can quickly discriminate whether the target pesticide exceeds the maximal residue limit just by naked eyes. To improve the performance of sensing platform, some important parameters like buffer condition and ssDNA have been investigated, and the peroxidase activity of hemin was further studied to verify the catalytic mechanism. The proposed sensing platform has a detection limit as low as 0.6 µg/L and displays good selectivity against other competitive pesticides. Moreover, the developed sensing platform also exhibits favorable accuracy and stability, indicating that it has potential applications in the detection of pesticide residues in agricultural products. Graphical abstract A novel colorimetric sensing platform based on oligonucleotides and pesticide regulation; the peroxidase catalytic activity of hemin was firstly reported for the ultrasensitive and selective detection of isocarbophos pesticide.

Exogenous brain-derived neurotrophic factor attenuates neuronal apoptosis and neurological deficits after subarachnoid hemorrhage in rats.

Brain-derived neurotrophic factor (BDNF) is a growth factor crucial for neuronal survival, while its role in subarachnoid hemorrhage (SAH)-induced neuronal apoptosis remains unclear. The aim of the present study was to investigate whether administering exogenous BDNF can protect against neuronal apoptosis and neurological deficits following SAH in a rat model. The BDNF level was found to be significantly decreased in the basal cortex at 6, 12, 24, 48 and 72 h following SAH. Exogenous BDNF significantly decreased the expression of Bax and reduced activation of caspase-3 and caspase-9 and the number of apoptotic neurons. Moreover, exogenous BDNF treatment significantly improved the neurological deficits at 72 h and long-term behavioral deficits (day 14) following SAH in a rat model. These findings indicate that exogenous BDNF attenuated SAH-induced neuronal injury in rats.

MicroRNA-133 inhibits the growth and metastasis of the human lung cancer cells by targeting epidermal growth factor receptor.

PURPOSE: Accounting for 25% of all cancers and 20% of the cancer related-mortality, lung cancer is one of the devastating types of malignancies. The main obstacles for successful lung cancer manipulation include late diagnosis, dearth of safer chemotherapy and lack of potent therapeutic targets. Evidence indicates that microRNAs (miRs) may prove essential therapeutic targets for the management of deadly diseases, including cancer. Herein the role of miR-133 was investigated in lung cancer and the therapeutic potential of miR-133 was explored. METHODS: qRT-PCR was used for expression profiling of miR-133 and epithermal growth factor receptor (EGFR) in normal lung cell line MRC5 and lung cancer cell lines SK-MES-1, A549, A427 and DMS-53. Cell Counting Kit-8 (CCK8) assay was used to monitor the proliferation rate. Flow cytometry was used for cell cycle analysis. Apoptosis was examined by DAPI and annexin V/propidium iodide (PI) staining. TargetScan analysis was performed to identify the potential target of miR-133 and western blot analysis was done to estimate the proteins' expression. RESULTS: miR-133 was significantly (p<0.05) downregulated in lung cancer. Overexpression of miR-133 in A549 lung cancer cells caused significant (p<0.05) inhibition of their proliferation via activation of apoptotic cell death, suggestive of the tumor suppressive role of miR-133. In addition, miR-133 overexpression also resulted in significant (p<0.05) suppression of A549 cell migration and invasion. TargetScan analysis indicated EGFR to be the potential target of miR-133 in A549 cells. Analysis of EGFR expression in lung cancer cell lines showed up to 4.6 fold upregulation of EGFR. However, miR-133 overexpression resulted in downregulation of EGFR expression. Furthermore, silencing of EGFR also resulted in inhibition of proliferation, migration and invasion of A549 cells. However, overexpression of EGFR could nullify the tumor suppressive effects of miR-133, indicating EGFR inhibition is essential for the miR-133-mediated inhibitory effects on A549 cell proliferation. CONCLUSION: Taken together, miR-133 acts as a tumor suppressor and may prove essential in the management of lung cancer.

A Novel Simple Puncture Positioning and Guidance System for Intracerebral Hematoma.

BACKGROUND: Minimally invasive surgical techniques may have beneficial effects on spontaneous intracerebral hemorrhage. Accurate localization of the hematoma and real-time guided puncture are more important in minimally invasive surgical procedures than in traditional craniotomy. Here, we introduce a novel simple puncture positioning and guidance system for intracerebral hematoma and demonstrate its utility for hematoma puncture surgery in a simulation experiment and series of patients. METHODS: We describe the device and use of the technique for hematoma puncture surgery in basal ganglia hematomas and report on the precision of the simulation experiments compared to that of freehand puncture, as well as its clinical application in 16 cases. RESULTS: The accuracy of this technique was superior to that of freehand puncture. All 16 patients underwent successful puncturing of the hematoma cavity or ventricles only once without any related complications. CONCLUSIONS: We demonstrate a novel simple puncture positioning and guidance system that has the advantages of simplicity, low-cost, device availability, and individual real-time guidance. We believe this system may be useful in resource-limited centers where navigation is not available.

Mitofusin2 regulates the proliferation and function of fibroblasts: The possible mechanisms underlying pelvic organ prolapse development.

The present study aimed to investigate the effects of Mitofusin2 (Mfn2) on the proliferation of human uterosacral ligament fibroblasts and on the expression of procollagen. We also aimed to identify the possible signal transduction pathway involved in the development of pelvic organ prolapse (POP). For this purpose, uterosacral ligaments were harvested from POP and non­pelvic organ prolapse (NPOP) patients for fibroblast culture. Cellular proliferation and the cell cycle were assessed following transduction with lentiviral vectors for the overexpression and suppression of Mfn2. The expression levels of the proteins Mfn2, procollagens, phosphoprotein 21 wild­type p53 activating fragment (p21Waf1), cyclin­dependent kinase 2 (CDK2), extracellular signal­regulated kinase1/2 (ERK1/2) and rapidly accelerated fibrosarcoma­1 (Raf­1) were examined. Overexpression of Mfn2 resulted in the decreased proliferation of cells and the induction of G0/G1 phase arrest. Concomitantly, the relative expression levels of procollagen proteins, CDK2 and the phosphorylation levels of ERK1/2 and Raf­1 proteins were notably decreased, while the levels of the p21waf1 protein were increased in the Mfn2 overexpressing group. Opposing results were reported cells following Mfn2 silencing via RNA interference. The results of the present study indicated that the cell cycle of the fibroblasts, their cellular proliferation and the levels of the procollagen proteins could be inhibited via the Ras­Raf­ERK axis as a result of the increased levels of Mfn2 during the development of POP.

Simultaneous fluorometric determination of the DNAs of Salmonella enterica, Listeria monocytogenes and Vibrio parahemolyticus by using an ultrathin metal-organic framework (type Cu-TCPP).

Ultrathin (<10 nm) nanosheets of a metal-organic framework (MOF-NSs) were prepared in high-yield and scalable production by a surfactant-assisted one-step method. The MOF-NSs possess distinguished affinity for ssDNA but not for dsDNA. This causes the fluorescence of the labeled DNA to be quenched. On binding to the target DNA (shown here for Salmonella enterica, Listeria monocytogenes and Vibrio parahemolyticus), the labeled duplex is released and the fluorescence of the label is restored. The labels Texas Red, Cy3 and FAM were used and give red, red or green fluorescence depending on the kind of pathogen. The detection limits are 28 pM, 35 pM and 15 pM for the gene segments of Salmonella enterica, Listeria monocytogenes and Vibrio parahemolyticus, respectively. Graphical abstract Schematic of an ultrasensitive fluorescent biosensor for multiplex detection of pathogenic DNAs based on ultrathin MOF nanosheets (type Cu-TCPP).

Surgical Management of Moderate Basal Ganglia Intracerebral Hemorrhage: Comparison of Safety and Efficacy of Endoscopic Surgery, Minimally Invasive Puncture and Drainage, and Craniotomy.

OBJECTIVE: To date, no standard surgical procedure has been proven effective for intracerebral hemorrhage (ICH), particularly deep hematomas. This retrospective study evaluated the effectiveness and safety of endoscopic surgery, minimally invasive puncture and drainage, and craniotomy for treating moderate basal ganglia ICH. METHODS: Patients with basal ganglia ICH (N = 177) were divided into 3 groups based on therapeutic intervention as follows: endoscopic surgery group (n = 61), minimally invasive puncture and drainage group (n = 60), and craniotomy group (n = 56). Patient characteristics at admission were recorded. Operative time; blood loss during operation; evacuation rate; postoperative complications secondary to perihematomal edema, including rebleeding, infectious meningitis, pulmonary infection, gastrointestinal bleeding, and epilepsy; mortality; and Glasgow Outcome Scale scores were compared among the 3 groups. RESULTS: Minimally invasive puncture and drainage was the least traumatic procedure and had the shortest operative time, but it could not remove the hematoma quickly; moreover, it had the highest rebleeding rate. Craniotomy was effective in removing the hematoma but resulted in marked trauma and had the highest incidence of pulmonary infection. Endoscopic surgery was safer and more effective than the other 2 surgical methods, with greater improvement in neurologic outcomes and no change in mortality. CONCLUSIONS: Minimally invasive neuroendoscopic management has the advantages of direct vision, efficient hematoma evacuation, and relatively good results. Endoscopic surgery may be a more promising approach for the treatment of moderate basal ganglia ICH.

Solution-Phase Synthesis of Platinum Nanoparticle-Decorated Metal-Organic Framework Hybrid Nanomaterials as Biomimetic Nanoenzymes for Biosensing Applications.

The synthesis of nanomaterials with specific properties and functions as biomimetic nanoenzymes has attracted extensive attention in the past decades due to their great potential to substitute natural enzymes. Herein, a facile and simple method for the preparation of platinum nanoparticle (PtNP)-decorated two-dimensional metal-organic framework (MOF) nanocomposites was developed. A ligand with heme-like structure, Fe(III) tetra(4-carboxyphenyl)porphine chloride (TCPP(Fe)), was applied to synthesize MOF nanosheets (denoted as Cu-TCPP(Fe) nanosheets) in high yield. Ultrathin Cu-TCPP(Fe) nanosheets with thickness less than 10 nm were used as a novel template for the growth of ultrasmall and uniform PtNPs. Significantly, the obtained hybrid nanomaterials (PtNPs/Cu-TCPP(Fe) hybrid nanosheets) exhibit enhanced peroxidase-like activity compared to PtNPs, Cu-TCPP(Fe) nanosheets, and the physical mixture of both due to the synergistic effect. On account of the excellent peroxidase-like activity of PtNPs/Cu-TCPP(Fe) hybrid nanosheets, we established a colorimetric method for sensitive and rapid detection of hydrogen peroxide. Furthermore, by combining with glucose oxidase, a cascade colorimetric method was established to further detect glucose with excellent sensitivity and selectivity.