Genetic analysis of potential biomarkers and therapeutic targets in ferroptosis from psoriasis.

Introduction: Ferroptosis is associated with multiple pathophysiological processes. Inhibition of ferroptosis has received much concern for some diseases. Nonetheless, there is no study comprehensively illustrating functions of ferroptosis-related genes (FRGs) in psoriasis. Methods: In this study, FRGs together with psoriasis-associated data were obtained in Ferroptosis Database (FerrDb) and gene expression omnibus (GEO) database separately. This work identified altogether 199 psoriasis-associated DE-FRGs, and they were tightly associated with immunity and autophagy modulation. Thereafter, the present study utilized SVM-RFE and LASSO algorithms to identify NR5A2, CISD1, GCLC, PRKAA2, TRIB2, ABCC5, ACSF2, TIMM9, DCAF7, PEBP1, and MDM2 from those 199 DE-FRGs to be marker genes. As revealed by later functional annotation, the marker genes possibly had important effects on psoriasis through being involved in diverse psoriasis pathogenesis-related pathways such as cell cycle, toll-like receptor (TLR), chemokine, and nod-like receptor (NLR) pathways. Moreover, altogether 37 drugs that targeted 11 marker genes were acquired. Besides, based on CIBERSORT analysis, alterations of immune microenvironment in psoriasis cases were possibly associated with PRKAA2, PEBP1, CISD1, and ACSF2. Discussion: Taken together, this work established the diagnostic potency and shed more lights on psoriasis-related mechanism. More investigations are warranted to validate its value in diagnosing psoriasis before it is applied in clinic.

Role of modulation frequency in a hybrid Q-switched Nd:LuVO4 laser with an acousto-optic modulator and a Cr4⁺:YAG saturable absorber.

This study presents a hybrid Q-switched Nd:LuVO4 laser with an acousto-optic modulator (AOM) and a Cr4⁺:YAG saturable absorber. The importance of the modulation frequency of the AOM in this dual-loss modulation system is examined with two distinct observations: (1) the periodic-ramp pulse characteristics with the modulation frequency, and (2) the existence of the intrinsic modulation frequency for minimizing the pulsewidth. The shortest pulsewidth and the highest peak power were 10.6 ns and 13.9 kW, respectively, at a pump power of 10 W. Compared with the passively Q-switched laser, which has a pulsewidth of 18.3 ns, the ratio of pulse compression reaches 42%. Finally, numerical simulations based on the rate equations are used to verify the experimental results.