Simultaneous pseudospin and valley topological edge states of elastic waves in phononic crystals made of distorted Kekulé lattices.

Topological metamaterials protected by the spatial inversion symmetry mainly support single type edge state, interpreted by either the quantum valley Hall effect or the quantum spin Hall effect. However, owing to the existence of the complicated couplings and waveform conversions during elastic wave propagation, realizing topologically protected edge states that support both pseudospin and valley degrees of freedom in elastic system remains a great challenge. Here, we propose a two-dimensional Kekulé phononic crystal (PC) that can simultaneously possess pseudospin- and valley-Hall edge states in different frequency bands. By inhomogeneously changing the elliptical direction in a Kekulé lattice of elliptical cylinders, three complete phononic bandgaps exhibiting distinct topological phase transitions can be obtained, one of which supports a pair of pseudospin-Hall edge states and the other hosts valley-Hall edge states in the low and high frequency regime. Furthermore, a sandwiched PC heterostructure and a four-channel cross-waveguide splitter are constructed to achieve selective excitation and topological robust propagation of pseudospin- and valley-momentum locking edge states in a single configuration. These results provide new possibilities for manipulating in-plane bulk elastic waves with both pseudospin and valley degrees of freedom in a single configuration, which has potential applications for multiband and multifunctional waveguiding.

Transcriptome analysis revealed the existence of family-specific regulation of growth traits in grass carp.

The grass carp (Ctenopharyngodon idella) is the world's most prolific freshwater fish. Little is known, however, about the functional genes and genetic regulatory networks that govern its growth traits. We created three grass carp families in this study by using two grass carp parents with fast-growing offspring and two grass carp parents with slow-growing offspring, namely the fast-growing × fast-growing family (FF), the slow-growing × slow-growing family (SS), and the fast-growing × slow-growing family (FS). Under the satiation and starvation feeding modes, the average body weight of these families' offspring exhibited a consistent ordering (FF > FS > SS). The transcriptomes of grass carp whole brain and hepatopancreas were then acquired for each family, and it was discovered that the number of differentially expressed genes (DEGs) in the different organs demonstrated family specificity. DEGs were mostly identified in the hepatopancreas of FF and the whole brain of SS, but they were more evenly distributed in FS. There were 14 DEGs that were found in all three families, including three that were negatively correlated in hepatopancreas (ahsg2, lect2) or in brain (drd5), and 11 that were positively connected in hepatopancreas (sycn, pabpc4, zgc:112294, cel, endou, ela2, prss3, zbtb41, ela3) or in brain (fabp7, endod1). The deletion of ahsg2 boosted the growth rate only in certain zebrafish, suggesting that the growth-promoting effects of ahsg2 varies among individuals. Furthermore, we examined the SNP in each family and conducted preliminary research on the probable genetic pathways of family-specific control of growth traits. The family specificity of the growth regulation mechanism of grass carp at the transcriptional level was revealed for the first time in this study, and it was discovered that growth differences among individuals in the FF family were primarily due to differences in nutrient metabolism, whereas growth differences among individuals in the SS family may be primarily due to differences in foraging ability caused by differences in brain development. This research adds to our understanding of the genetic regulatory mechanism of grass carp growth.

Recent advances in topological elastic metamaterials.

Topological elastic metamaterials have emerged as a new frontier in the quest of topological phases in condensed matter physics. Their exotic topological properties open a wealth of promising engineering-oriented applications that are difficult to realize with traditional elastic metamaterials, such as robust and defect insensitive waveguiding, signal sensing, and splitting. In this review, we retrospectively examine the underlying physical concept of topologically ordered states of elastic waves, starting from the one-dimensional example based on the Su-Schrieffer-Heeger model. We then move on to two-dimensional topological metamaterials, discussing elastic analogues of quantum Hall, pseudospin-Hall, valley-Hall phases. Finally, we survey the latest developments in the field including three-dimensional elastic topological phases and higher-order topological insulators. Altogether, this paper provides a comprehensive overview of the flourishing research frontier on topological elastic metamaterials, and highlights prominent future directions in this field.

Pseudospins and topological edge states for fundamental antisymmetric Lamb modes in snowflakelike phononic crystal slabs.

The topological transport of Lamb wave in phononic crystal slabs provides a great potential in reinforcing nondestructive testing, high sensitivity sensing, and information processing. In this paper, the authors investigate the pseudospins edge states of fundamental antisymmetric Lamb waves in a snowflakelike phononic slab. Significantly, the fourfold Dirac degeneracy for antisymmetric Lamb mode is accidentally formed at the Γ point with the critical angle of the snowflakelike holes, which does not require the folding of the lattices. Meanwhile, based on the rotating-scatterer mechanism, the mirror symmetry is broken and the topological multipole phase transitions are well induced during the gradual change of the scattering strength among the scatterers with the rotation angle. The topologically protected edge states and its unidirectional robust propagation are further demonstrated. The proposed topological phononic slabs will be a more hopeful option to apply in engineering practices.

Thermally tunable topological edge states for in-plane bulk waves in solid phononic crystals.

The remarkable properties of topological insulators have inspired numerous studies on topological transport for bulk waves, but the demonstrations of topological edge states with tunable frequency are few attempts. Here, we report on the active frequency tunability of topologically protected edge states for in-plane bulk waves by applying a thermal field. We find that the center frequency of topological band gap is shifted down and the band width is enlarged as the temperature increases. Meanwhile, the frequency range of topologically protected edge states is also shifted to low frequency region with the higher temperature. Furthermore, the robust propagation of in-plane bulk waves along a desired path is demonstrated within different frequency bands. The tunable frequency for both topological band gaps and topologically protected edge states achieves the active control of the transport for in-plane bulk waves, which may dramatically facilitate practical applications of novel phononic devices.

Topologically protected edge states for out-of-plane and in-plane bulk elastic waves.

Topological phononic insulators (TPnIs) show promise for application in the manipulation of acoustic waves for the design of low-loss transmission and perfectly integrated communication devices. Since solid phononic crystals exist as a transverse polarization mode and a mixed longitudinal-transverse polarization mode, the realization of topological edge states for both out-of-plane and in-plane bulk elastic waves is desirable to enhance the controllability of the edge waves in solid systems. In this paper, a two-dimensional (2D) solid/solid hexagonal-latticed phononic system that simultaneously supports the topologically protected edge states for out-of-plane and in-plane bulk elastic waves is investigated. Firstly, two pairs of two-fold Dirac cones, respectively corresponding to the out-of-plane and in-plane waves, are obtained at the same frequency by tuning the crystal parameters. Then, a strategy of zone folding is invoked to form double Dirac cones. By shrinking and expanding the steel scatterer, the lattice symmetry is broken, and band inversions induced, giving rise to an intriguing topological phase transition. Finally, the topologically protected edge states for both out-of-plane and in-plane bulk elastic waves, which can be simultaneously located at the frequency range from 1.223 to 1.251 MHz, are numerically observed. Robust pseudospin-dependent elastic edge wave propagation along arbitrary paths is further demonstrated. Our results will significantly broaden its practical application in the engineering field.

Multi-objective optimization of asymmetric acoustic transmission with periodical structure.

Asymmetric acoustic wave propagation is important for control and manipulation of the acoustic wave signals in various devices. However, previous approach to find optimal asymmetric acoustic transmission (AAT) is through repeatedly adjusting the geometrical parameters, thus causing time-consuming. Here we propose a study on the multi-objective optimization of the AAT, aiming to achieve the widest working frequency range (fr) and the highest transmittance peak (η) with regard to the design variables. For this purpose, the Radial Basis Function (RBF) neural work and finite element (FE) method are applied to obtain the valuable data in the procedure. Furthermore, local sensitivity analysis of design parameters on the fr and η are analyzed. Ultimately, the Non-Dominated Sorting Genetic Algorithm II (NSGA-II) is adapted for getting the Pareto-optimal solutions. The optimization results show great improvement for the overall performance of the AAT, which could be potentially significant in designing various sound devices.

Simultaneous multi-band valley-protected topological edge states of shear vertical wave in two-dimensional phononic crystals with veins.

The introduction of the concept of valley pseudospin to phononic crystals has made a remarkable topologically protected interface transport of sound, which opens a novel research area referred to as valley Hall topological insulators. Here, we demonstrate the simultaneous multi-band edge states of shear vertical waves in two-dimensional phononic crystals with veins. The multi-band edge states are topologically valley-protected and are obtained by simultaneously gapping multiple Dirac points at K (or K') under the inversion symmetry breaking. As the relative radius of the two adjacent steel columns varies, the band diagram undergoes a topological transition which can be characterized by topological charge distributions and opposite valley Chern numbers. Subsequently, the vortex chirality of the bulk valley modes is unveiled. With numerical simulations, simultaneous multi-band valley dependent edge states and the associated valley-protected backscattering suppression around the curved waveguide are further demonstrated. Our work could become a promising platform for applications of multi-functional topological acoustic devices.

Tunable Lamb wave band gaps in two-dimensional magnetoelastic phononic crystal slabs by an applied external magnetostatic field.

This paper theoretically investigates the band gaps of Lamb mode waves in two-dimensional magnetoelastic phononic crystal slabs by an applied external magnetostatic field. With the assumption of uniformly oriented magnetization, an equivalent piezomagnetic material model is used. The effects of magnetostatic field on phononic crystals are considered carefully in this model. The numerical results indicate that the width of the first band gap is significantly changed by applying the external magnetic field with different amplitude, and the ratio between the maximum and minimum gap widths reaches 228%. Further calculations demonstrate that the orientation of the magnetic field obviously affects the width and location of the first band gap. The contactless tunability of the proposed phononic crystal slabs shows many potential applications of vibration isolation in engineering.

Lamb waves in phononic crystal slabs: truncated plane parallels to the axis of periodicity.

A theoretical study is presented on the propagation properties of Lamb wave modes in phononic crystal slabs consisting of a row or more of parallel square cylinders placed periodically in the host material. The surfaces of the slabs are parallel to the axis of periodicity. The dispersion curves of Lamb wave modes are calculated based on the supercell method. The finite element method is employed to calculate the band structures and the transmission power spectra, which are in good agreement with the results by the supercell method. We also have found that the dispersion curves of Lamb waves are strongly dependent on the crystal termination, which is the position of the cut plane through the square cylinders. There exist complete or incomplete (truncated) layers of square cylinders with the change of the crystal termination. The influence of the crystal termination on the band gaps of Lamb wave modes is analyzed by numerical simulations. The variation of the crystal termination leads to obvious changes in the dispersion curves of the Lamb waves and the widths of the band gaps.

Cloning, expression, and characterization of an alkaline thermostable GH11 xylanase from Thermobifida halotolerans YIM 90462T.

A xylanase gene (thxyn11A) from the Thermobifida halotolerans strain YIM 90462(T) was cloned and expressed in Escherichia coli. The open reading frame (ORF) of thxyn11A has 1,008 bp encoding a mature xylanase with a high degree of similarity (80 %) to the xylanase from Nocardiopsis dassonvillei subsp. dassonvillei DSM 43111. This enzyme (Thxyn11A) also possesses a glycosyl hydrolases family 11 (GH11) domain and a high isoelectric point (pI = 9.1). However, Thxyn11A varies from most GH11 xylanases, due to its large molecular mass (34 kDa). Recombinant Thxyn11A demonstrated a strong pH and temperature tolerance with a maximum activity at pH 9.0 and 70 °C. Xylotriose, the end-product of xylan hydrolysis by Thxyn11A, serves as a catalyst for hemicellulose pretreatment in industrial applications and can also function as a food source or supplement for enterobacteria. Due to its attractive biochemical properties, Thxyn11A may have potential value in many commercial applications.

Laceyella sediminis sp. nov., a thermophilic bacterium isolated from a hot spring.

A thermophilic bacterium, designated strain RHA1(T), was isolated from a sediment sample collected from a hot spring in Tengchong county, Yunnan province, south-west China, and was characterized by using a polyphasic approach. Based on its phenotypic and phylogenetic characteristics, strain RHA1(T) was affiliated to the genus Laceyella. The strain formed white aerial and yellow-white substrate mycelia, bearing single endospores on short sporophores. The cell-wall peptidoglycan contained meso-diaminopimelic acid. Whole-cell hydrolysates contained ribose and glucose. The major fatty acids were iso-C(15:0) (62.39%) and anteiso-C(15:0) (17.55%)(.) The predominant menaquinone was MK-9. The G+C content of the genomic DNA of strain RHA1(T) was 47.9 mol%. Based on DNA-DNA hybridization data, chemotaxonomic characteristics and differential physiological properties, strain RHA1(T) is considered to represent a novel species of the genus Laceyella, for which the name Laceyella sediminis sp. nov. is proposed; the type strain is RHA1(T) (=DSM 45263(T)=CCTCC AA 208058(T)).

Cloning, expression and characterization of an alkaline thermostable GH9 endoglucanase from Thermobifida halotolerans YIM 90462 T.

The endoglucanase gene, thcel9A, from Thermobifida halotolerans YIM 90462(T) was cloned and expressed in Escherichia coli BL 21(DE). The 2895-bp full-length gene encodes a 964-residue polypeptide (Thcel9A) containing a catalytic domain belonging to glycosyl hydrolases (GH) family 9. Phylogenetic analysis indicated that Thcel9A is closely related to Cel9A of Thermobifidafusca YX. Thcel9A was purified from the culture supernatant by Ni(2+)-affinity chromatography and the purified enzyme exhibited optimal activity at 55°C and pH 8.0. Substrate specificity assays showed that it not only had CMCase activity, but also hydrolase activity on microcrystalline cellulose and filter paper. These properties suggested that Thcel9A is a classical GH9 group A endoglucanase.

Plate-mode waves in phononic crystal thin slabs: mode conversion.

We have computed the dispersion curves of plate-mode waves propagating in periodic composite structures composed of isotropic aluminum cylinders embedded in an isotropic nickel background. The phononic crystal has a square symmetry and the calculation is based on the plane-wave expansion method. Along GammaX or GammaM directions, shear-horizontal modes do not couple to the Lamb wave modes which are polarized in the sagittal plane. Whatever the direction of propagation in between GammaX and GammaM, shear-horizontal modes convert to Lamb waves and couple with the flexural and dilatational modes. This phenomenon is demonstrated both through the mode splitting in the lower-order symmetric band structure and through the calculation of all three components of the particle displacements. The phononic case is different from the pure isotropic plate case where shear-horizontal waves decouple from Lamb waves whatever the direction of propagation.