CXCL8 delivered by plasma-derived exosomes induces the symptoms of post-traumatic stress disorder through facilitating astrocyte-neuron communication.

Extracellular vesicles (EVs) play an important role in post-traumatic stress disorder (PTSD). This study is aimed to investigate the possible molecular mechanism of CD63 mediating CXCL8 delivery via EVs to affect astrocyte-neuron communication in PTSD. The neuron-derived EVs (NDEVs) and astrocyte-derived EVs (ADEVs) were isolated from plasma in PTSD patients. Next, the uptake of EVs by neurons was assessed. Following determination of the interaction between CD63 and CXCL8, gain- and loss-of-function experiments were performed in astrocytes. Finally, a PTSD mouse model was established using the single prolonged stress and electric foot shock to confirm the effects of plasma-derived EVs delivering CXCL8 on anxiety- and depression-like behaviors in PTSD mice. EVs derived from plasma of PTSD patients aggravated anxiety- and depression-like behaviors in PTSD mice. CXCL8 was a key gene upregulated in both NDEVs and ADEVs from plasma of PTSD patients, which could be delivered into EVs by CD63. Meanwhile, CXCL8 was also highly expressed in plasma-derived EVs. In vivo experiments also verified that plasma-derived EVs could enhance astrocyte-neuron communication by delivering CXCL8, and silencing of CXCL8 ameliorated anxiety- and depression-like behaviors in PTSD mice. Taken together, CD63 promotes delivery of CXCL8 via EVs to induce PTSD by enhancing astrocyte-neuron communication, suggesting the potential of CD63 mediating delivery of CXCL8 via EVs as a therapeutic target for PTSD.

Development of polyvinyl alcohol-based carbon nano fiber sheet for thermal interface material.

Polyvinyl alcohol (PVA)-based carbon nanofiber (CNF) sheets are fabricated as an innovative thermal interface material (TIM), which is a potential substitute for traditional TIMs. Five types of PVA-based CNF sheets were fabricated at different mass ratios of PVA:vapor-grown carbon fiber (VGCF) (1:0.100, 1:0.070, 1:0.050, 1:0.030, 1:0.025). The thickness of the PVA-based CNF sheets was 30-50 µm, which was controlled by the amount of VGCF. The microstructure of the CNF sheets indicated that VGCFs were arranged in random directions inside the sheet, and PVA was formed as a membrane between two VGCFs. However, many pores were found to exist between the VGCFs. The porosity of the PVA-based CNF sheets decreased from 25 to 13% upon decreasing the mass ratio of VGCF from 43.38 to 16.13%. The density and Shore hardness of all CNF sheets were 1.03-1.15 × 106 g m-3 and 82.4-85.0 HS, respectively. The highest thermal conductivity, measured as the mass ratio of PVA:VGCF, was achieved at 1:0.05, with the in-plane thermal conductivity of the fabricated sheet being 14.3 W m-1 k-1.