Flexible molecularly imprinted glucose sensor based on graphene sponge and Prussian blue.

To enhance the sensitivity of flexible glucose sensors made with 3-aminophenylboronic acid and pyrrole as functional molecules and a carbon tri-electrode as substrate, graphene sponge (GS) and Prussian blue (PB) were used to enhance the charge transfer between the molecularly imprinted cavities and the electrodes. Electrochemical impedance spectroscopy and cyclic voltammetry showed that modifying the electrode with GS and PB significantly reduced the charge transfer impedance and increased the redox current of the sensor. The sensor has a sensitivity of up to 25.81 µA⋅loge (µM)-1⋅cm-2 for the detection of glucose using differential pulse voltammetry in the range of 7.78 to 600 µM, with a low detection limit of 1.08 µM (S/N = 3). When the pH varies in the range of 5.5 to 7.5, the sensor maintains a certain level of stability for glucose detection. The presence of lactic acid, urea, and ascorbic acid had minimal impact on glucose detection by the sensor. After 20 days of storage at room temperature, the sensor maintains 80 % efficiency. This study supports the development of wearable glucose sensors with high sensitivity, specificity, and stability through molecular imprinting.

Toward understanding the interaction of shale gas-water-carbon nexus in Sichuan-Chongqing region based on county-level water security evaluation.

This study develops a comprehensive framework for understanding the interaction of shale gas-water-carbon nexus in Sichuan-Chongqing region. Within this framework, a county-level water security index (WSI) evaluation system is structured. Spatial autocorrelation model and spatial matching degree model are integrated to illustrate the spatial agglomeration characteristics of water security and the water-carbon relationship, respectively. The impacts of shale gas development on water security and carbon emissions are evaluated based on identification of shale well productivity. Results show that about 25.17% of counties with WSI < 0.4 (unsafe), especially in the eastern region. The central cities (such as Chengdu and Neijiang) should take active steps to reach a safety threshold (WSI ≥ 0.6). Population growth can accelerate water security deterioration through uncertainty analysis. Moreover, the spatial matching degree between WSI and carbon emissions in most cities is extremely poor (< 0.5), implying that these cities should optimize their energy structure and promote green transformation. Water used for shale gas extraction can hardly be ignored from a county-scale perspective, especially in Tongliang, Tongnan, and Jianyang. The future shale gas development would pose a threat to the regional climate.

Promoted electrocatalytic hydrogenation of furfural in a bi-phasic system.

The promoted electrocatalytic hydrogenation of biomass-derived furfural to 2-methylfuran is for the first time identified in a water/oil bi-phasic system, in which the oil phase can quickly separate hydrophobic products from the electrode/electrolyte interfaces, resulting in a beneficial equilibrium toward hydrodeoxygenation.

Further mitigating carbon footprint pressure in urban agglomeration by enhancing the spatial clustering.

The increasing environmental pressure of anthropogenic CO2 emissions is impeding the sustainability of urban agglomerations (UAs). Recent research has shown that the spatial clustering of UA elements reduces CO2 emissions but underestimates its impact on vegetation carbon sequestration. Using an extended IPAT equation analysis framework and the Logarithmic Mean Divisia Index decomposition approach, this study revealed the positive effects of the economy and population spatial clustering on carbon footprint pressure (CFP) mitigation. Specifically, improving economic spatial clustering mitigated the rise in UA's CFP caused by affluence and population growth. Furthermore, population clustering in core cities effectively mitigated CFP in neighboring cities. Additionally, we found that the efficiency improvement, i.e., the decrease in the ratio of carbon emissions and gross domestic product, should be the dominant driver of CFP mitigation, followed by improved vegetation carbon sequestration. However, these drivers have limited future potential. We believe that by improving UA's spatial clustering of the economy and population, future urban environmental pressures and climate risks will be mitigated.

Functional Genome Analyses Reveal the Molecular Basis of Oil Accumulation in Developing Seeds of Castor Beans.

Castor (Ricinus communis L.) seeds produce abundant ricinoleic acid during seed maturation, which is important for plant development and human demands. Ricinoleic acid, as a unique hydroxy fatty acid (HFA), possesses a distinct bond structure that could be used as a substitute for fossil fuels. Here, we identified all homologous genes related to glycolysis, hydroxy fatty acid biosynthesis, and triacylglycerol (TAG) accumulation in castor seeds. Furthermore, we investigated their expression patterns globally during five seed development stages. We characterized a total of 66 genes involved in the glycolysis pathway, with the majority exhibiting higher expression levels during the early stage of castor bean seed development. This metabolic process provided abundant acetyl-CoA for fatty acid (FA) biosynthesis. Subsequently, we identified 82 genes involved in the processes of de novo FA biosynthesis and TAG assembly, with the majority exhibiting high expression levels during the middle or late stages. In addition, we examined the expression patterns of the transcription factors involved in carbohydrate and oil metabolism. For instance, RcMYB73 and RcERF72 exhibited high expression levels during the early stage, whereas RcWRI1, RcABI3, and RcbZIP67 showed relatively higher expression levels during the middle and late stages, indicating their crucial roles in seed development and oil accumulation. Our study suggests that the high HFA production in castor seeds is attributed to the interaction of multiple genes from sugar transportation to lipid droplet packaging. Therefore, this research comprehensively characterizes all the genes related to glycolysis, fatty acid biosynthesis, and triacylglycerol (TAG) accumulation in the castor and provides novel insight into exploring the genetic mechanisms underlying seed oil accumulation in the endosperm of castor beans.

GJA1 reverses arsenic-induced EMT via modulating MAPK/ERK signaling pathway.

Arsenic is known as a well-established human carcinogen. Gap Junction Protein Alpha 1 (GJA1) is a multifunction protein that forms gap junction channels and is important for intercellular communication. Recently, its aberrant expression has been shown to associate with cancer recurrence and metastatic spread. However, whether GJA1 plays a role in arsenic carcinogenesis remains unknown. Here, we demonstrated that chronic exposure of human bronchial epithelial BEAS-2B cells to sodium arsenite promoted epithelial-mesenchymal transition (EMT) via increasing the expression of EMT inducer S100A4 and activation of MAPK/ERK signaling. In vitro and in vivo experiments showed that chronic exposure to sodium arsenite reduced GJA1 expression. Forced expression of GJA1 inhibited sodium arsenite-induced EMT via suppressing MAPK/ERK signaling whereas GJA1 knockdown produced an opposite effect. Intriguingly, chronic exposure to sodium arsenite increased autophagy flux. Inhibition of autophagy by pharmacological intervention or genetic deletion of autophagy core gene Beclin-1 upregulated GJA1 expression. These results suggested that GJA1 restrained the carcinogenic effect of sodium arsenite by limiting MAPK/ERK signaling, and GJA1 expression was decreased by arsenic-activated autophagy. In addition, interventions directed at enhancing the level or functional activity of GJA1 could be of preventive and therapeutic interest.

Integrating water-related disaster and environment risks for evaluating spatial-temporal dynamics of water security in urban agglomeration.

Water security is a compound concept coupling multi-dimensional perspectives, such as resource utilization, environmental protection, and disaster prevention. With this concern, this study focuses on the spatial-temporal dynamics of water security with considering water disaster risk index (WDRI), water environment risk index (WERI), and water supply-demand. WERI centers on risk source's hazard, control effectiveness, and risk receptor's vulnerability. Indices related to hazard, exposure, and vulnerability are used for evaluating WDRI. A multi-objective fuzzy membership function is presented for determining the indices' weight, and the distribution pattern of water security is illustrated based on cluster analysis. A real-world case study of Beijing-Tianjin-Hebei urban agglomeration (BTHUA) is given for verifying availability of the evaluation framework. Results indicate the general water security in BTHUA with a critical safe state yet a downward trend. Opposite change characteristics of water security exist between its southern and northern cities. WDRI and WERI show the trends of increasing (with a growth rate of 0.48%) and decline (with an average decrement rate of 0.56%), respectively. Beijing has high-value WDRI and WERI, and the order of WDRI and WERI is presented as follows: Beijing (0.67) > Tianjin (0.54) > Hebei (0.33) and Beijing (0.69) > Tianjin (0.58) > Hebei (0.16), respectively. Cluster analysis reveals a poor match relation between water security and regional socio-economic development. Areas with high-level economic development (e.g., Beijing and Tianjin) have poor environmental performances, with WDRI and WERI of 0.54 ~ 0.68 and 0.57 ~ 0.70, respectively. Additionally, water resources overload index of BTHUA is 8.513, which is higher than Chengdu-Chongqing urban agglomeration (1.431), Triangle of Central China (0.228), and Yangtze River Delta urban agglomeration (0.742). Findings can provide a theoretical reference for promoting sustainable utilization of water resources in BTHUA and the other areas with prominent water problems.

Spatial-temporal collaborative relation among ecological footprint depth/size and economic development in Chengyu urban agglomeration.

This is the first attempt to simultaneously apply an improved three-dimensional ecological (EF3D) model and multivariate spatial-temporal collaborative relation model for evaluating the inter-regional ecological sustainability. Capital flow consumption and capital stock depletion can be tracked based on identification of variations in ecological footprint size (EFsize) and depth (EFdepth). Results display an unsustainable natural capital stock utilization in Chengyu urban agglomeration, especially in Neijiang, Chengdu, and Deyang with EFdepth higher than 35. There exists an obvious regional complementarity between EFsize and EFdepth, where cities with rich resources (or poor resources) are generally characterized as high EFsize and low EFdepth (or low EFsize and high EFdepth). The EF3D is slightly lower than traditional ecological footprint in value owing to its more concerns of intergenerational fairness and intrageneration fairness. Moreover, the spatial-temporal binary collaborative relation among EFsize, EFdepth, and GDP is generally higher than 0.6 with a medium level. Their temporal multivariate collaborative relation mostly lies in the intervals [0.30, 0.50] with an annual change rate of 10.22%, while slight variations exist in their spatial multivariate collaborative relation. GDP has a significant impact on the temporal collaborative relation of EFsize and EFdepth in Dazhou, Leshan, Luzhou, Mianyang, and Nanchong. There are slight impacts of EFdepth on the temporal collaborative relation of EFsize and GDP; meanwhile, GDP has a less effect on the spatial collaborative relation of EFsize and EFdepth. Findings can provide a reference for the coordinated development of social economy and ecological environment.

Energy information flow-based ecological risk transmission among communities within the heavy metals contaminated soil system.

An energy information flow-based ecological risk assessment framework (EIF-ERA) is developed for identifying ecological risk transmission rules among communities (i.e., vegetation E1, herbivorous animals E2, soil microorganisms E3, and carnivorous animals E4) within the heavy metals contaminated soil system. This framework is integrated with numerous techniques of carcinogenic risk evaluation, ecological risk assessment (ERA), and Monte Carlo simulation. Stepwise quadratic response surface analysis (SQRSA) is employed for reflecting the relation between contaminants' concentration and comprehensive risk. Two scenarios with respect to the environmental quality standards (scenarios 1) and carcinogenic risk reversion (scenarios 2) are merged into the EIF-ERA. A real-world mining area in Xinglong County in Chengde is selected to verify the developed framework's effectiveness. Results reveal that E3 is considered as the most sensitive community when contaminant interference occurs, and its 62.3% and 37.7% of comprehensive risk are contributed by initial and direct risks, respectively. Other communities can receive direct risk through control allocation (CA). Monte Carlo anlysis shows that there are 7.68% and 20.25% increase in the initial risk of Cd and Pb when their quantile statistics increase from 70% to 90%. Determination of an appropriate screening value is vital for contaminated mining soil remediation due to its inefficiency of remediation funds, especially when considering the trict standards of contaminants' concentration within scenarios 1. The surrogates obtained from the SQRSA display the relation of contaminant concentration and comprehensive risks with the adjusted R2 greater than 0.77. These findings can be in support of system design, risk assessment, and site remediation.

Hepatic Steatosis: CT-Based Prevalence in Adults in China and the United States and Associations With Age, Sex, and Body Mass Index.

BACKGROUND. Calibrated CT fat fraction (FFCT) measurements derived from un-enhanced abdominal CT reliably reflect liver fat content, allowing large-scale population-level investigations of steatosis prevalence and associations. OBJECTIVE. The purpose of this study was to compare the prevalence of hepatic steatosis, as assessed by calibrated CT measurements, between population-based Chinese and U.S. cohorts, and to investigate in these populations the relationship of steatosis with age, sex, and body mass index (BMI). METHODS. This retrospective study included 3176 adults (1985 women and 1191 men) from seven Chinese provinces and 8748 adults (4834 women and 3914 men) from a single U.S. medical center, all drawn from previous studies. All participants were at least 40 years old and had undergone unenhanced abdominal CT in previous studies. Liver fat content measurements on CT were cross-calibrated to MRI proton density fat fraction measurements using phantoms and expressed as adjusted FFCT measurements. Mild, moderate, and severe steatosis were defined as adjusted FFCT of 5.0-14.9%, 15.0-24.9%, and 25.0% or more, respectively. The two cohorts were compared. RESULTS. In the Chinese and U.S. cohorts, the median adjusted FFCT for women was 4.7% and 4.8%, respectively, and that for men was 5.8% and 6.2%, respectively. In the Chinese and U.S. cohorts, steatosis prevalence for women was 46.3% and 48.7%, respectively, whereas that for men was 58.9% and 61.9%, respectively. Severe steatosis prevalence was 0.9% and 1.8% for women and 0.2% and 2.6% for men in the Chinese and U.S. cohorts, respectively. Adjusted FFCT did not vary across age decades among women or men in the Chinese cohort, although it increased across age decades among women and men in the U.S. cohort. Adjusted FFCT and BMI exhibited weak correlation (r = 0.312-0.431). Among participants with normal BMI, 36.8% and 38.5% of those in the Chinese and U.S. cohorts, respectively, had mild steatosis, and 3.0% and 1.5% of those in the Chinese and U.S. cohorts, respectively, had moderate or severe steatosis. Among U.S. participants with a BMI of 40.0 or greater, 17.7% had normal liver content. CONCLUSION. Steatosis and severe steatosis had higher prevalence in the U.S. cohort than in the Chinese cohort in both women and men. BMI did not reliably predict steatosis. CLINICAL IMPACT. The findings provide new information on the dependence of hepatic steatosis on age, sex, and BMI.

Evaluating the Comprehensive Benefit of Urban Renewal Projects on the Area Scale: An Integrated Method.

Globally, the challenges facing cities regarding urban decay, insufficient urban function, and fragmented urban development are enormous. Under this context, urban renewal provides opportunities to address these challenges and enhance urban sustainability. Thus, promoting urban renewal projects and improving their performance is a global topic. In many circumstances, urban renewal is planned and initiated on the project scale, but on the area scale, overall coordination of the projects can bring about comprehensive benefits to urban areas on a macro view. In practice, it still lacks a systematic evaluation approach to obtain a clear picture of such comprehensive benefits. In academia, the existing research studies are mainly focused on single-project evaluation. An integrated framework that provides a holistic assessment of area-scale project benefits is missing. Few fully consider the coupling coordination benefits between several urban renewal projects from an area-scale perspective. Thus, this paper aims to propose a framework for integrating an indicator evaluation system through a hybrid entropy weight method with Back Propagation (BP) neural network methods to evaluate the comprehensive benefit of urban renewal projects on the area scale, which is the level at which most development area-scale renewal projects take place in a city. The feasibility and effectiveness of this proposed framework are then verified in a case study of Chongqing, China. The results indicate that the proposed method that integrated multi-project characteristics can contribute to a bigger picture of benefit evaluation of urban renewal based on an area scale perspective. This therefore provides not only guidance for urban planners and policymakers to make better decisions, but also new insight for benefit evaluation in the field of urban development.

A system-scale environmental risk analysis with considering a conceptual conversion from material/energy flow to information flow under uncertainties.

A conceptual conversion from material/energy flow to information flow is presented in this study for evaluating network environment analysis (NEA) within the naphthalene-contaminated groundwater ecosystems under stochastic-fuzzy uncertainties. Four components (i.e., vegetation, herbivore, soil microorganism, and carnivore) are considered into the NEA framework for quantifying their direct and integral ecological risks. Carcinogenic risk related to human health concern is also evaluated under four remediation periods. The developed method is then applied to a power plant site. Results reveal that the average naphthalene concentration after pump-and-treat treatment would significantly decrease from 8.672 to 1.232 µg/L when remediation period extends to 10 years. The probabilities of suffering from carcinogenic risk would reach 0.9862, 0.9566, 0.8746, and 0.6142 under different remediation periods. Soil microorganism would receive more input risk than vegetation owing to its higher vulnerability. Although the upper-layer components (such as herbivore and carnivore) are not exposed to risk sources, they would gradually accumulate to a high-level ecological risk through food chains. Sensitivity analysis shows that variations in standard boundaries would have a significant impact on the risks of all components within groundwater ecosystems. This study can offer a novel perspective and methodology for comprehensively assessing the system-scale environment risks.

Development of an oil spill model adaptable to exposure and submergence conversion of tidal flats: A case study in the Changjiang Estuary.

Through the application of the Water Pollution Emergency Response System (WPERS) in the Changjiang Estuary, it was found that tidal flats significantly affect the trajectory of an oil film, and the deposition of the oil film on tidal flats is remarkable. In this study, the Estuarine Oil Spill Model (EOSM) was developed to simulate the process of the oil film landing on tidal flats. The Lagrangian particle algorithm and oil fate experience algorithms were adopted. An algorithm for generating dynamic shorelines was also involved. Compared to the oil spill algorithm without wet-dry functionality, the spatial distribution of the oil film deposition simulated in the EOSM better matched the reanalysis data of the satellite remote sensing image. This indicated that the dynamic wet-dry switch phenomenon of tidal flats could be involved in the simulation of offshore oil spills to improve the accuracy of accident prediction and ecological loss assessment. PLAIN LANGUAGE SUMMARY: On December 30th, 2012, a vessel carrying 400 tons of heavy oil sank in the Changjiang Estuary, China. The heavy oil leaked at around 4 am the next day. In this study, a new oil spill model was developed to simulate the process of an oil film landing on land and tidal flats. An algorithm for generating the dynamic shorelines in an oil spill simulation was adapted for the wet-dry switch of tidal flats. The landing distribution of the oil film in this new model was well matched to the satellite image for this accident. This indicated that the dynamic wet-dry switch phenomenon of tidal flats could be involved in the simulation of oil spills offshore, to improve the accuracy of accident prediction and ecological loss assessment. The research results could provide some new ideas for the development of offshore oil spill simulation technology.

Fairness analysis and compensation strategy in the Triangle of Central China driven by water-carbon-ecological footprints.

This study proposes water-carbon-ecological footprints to form footprint family indicators for identifying the ecological compensation and regional development equilibrium in the Triangle of Central China (TOCC). The occupation of natural capital stock and flow consumption can be illustrated using a three-dimensional ecological footprint model, and Gini coefficient is integrated into the evaluation framework for fairness measurement from various aspects. Quantificational ecological compensation standards can be given with considering indicators associated with ecological resource conversion efficiency and willingness to pay. Results reveal that ecological and carbon footprints in the TOCC demonstrate rising trends from 2000 to 2015, while its water footprint presents a fluctuating trend. The majority of average Gini coefficients exceed the threshold value of 0.4 under different footprints, thereby indicating poor overall fairness of regional development. Water footprint in Jingmen, Xiangtan, and Yichun show relatively higher compensation expenses, while Yichang, Zhuzhou, and Fuzhou exhibit higher received compensation values compared with other cities. Carbon footprint in Wuhan, Loudi, and Xinyu indicate high compensation expenses due to their overuse of biological resources. Maximum amounts of compensation expense appear in Nanchang and Wuhan from the perspective of ecological footprint. This study can provide a theoretical reference for sustainable development in the TOCC by performing a comparative analysis with Beijing-Tianjin-Hebei urban agglomeration and developed countries.

Analysis of risk factors of bladder neck contracture following transurethral surgery of prostate.

BACKGROUNDS: The aim of the present study was to investigate the perioperative parameters associated with bladder neck contracture (BNC) after transurethral surgery of the prostate and to compare the incidence of BNC after transurethral resection of the prostate (TURP) or Thulium vaporesection (resection group) versus Thulium vapoenucleation or enucleation of the prostate (enucleation group). METHODS: Between March 2008 and March 2020, 2363 patients received TURP and 1656 patients received transurethral surgery of the prostate with Thulium laser (ThuP) at Mackay Memorial Hospital. A total of 62 patients developed BNC. These BNC patients were age-and operation-matched to 124 randomly sampled TURP/ThuP controls without BNC. A 1:1 propensity score matching model was used to evaluate the difference in incidence of BNC. RESULTS: Our study demonstrated that a greater proportion of BNC patients had history of cerebrovascular accidents (11/62 vs. 7/124, p = 0.009), coronary artery disease (14/48 vs. 16/108, p = 0.03), chronic kidney disease (14/62 vs. 11/124, p = 0.01), and two or more comorbidities (29/62 vs. 27/124, p = 0.001) compared with NBNC patients. Multivariate analysis showed that smaller prostate volume (OR 0.96 (0.94-0.99), p = 0.008) and recatherization (OR 5.6 (1.02-30.6), p = 0.047) were significantly associated with BNC. A ROC curve predicted that a prostate volume < 42.9 cm3 was associated with a notably higher rate of BNC. The propensity score matching model reported there was no difference in incidence between resection and enucleation groups. CONCLUSION: This study demonstrated that incidence of BNC was the same in different surgical techniques and that low prostate volume, recatherization and ≥ 2 comorbidities were positively correlated with the development of BNC after TURP or ThuP.

Interleukin 33 mediates hepatocyte autophagy and innate immune response in the early phase of acetaminophen-induced acute liver injury.

Despite interleukin 33 (IL-33) functions as an "alarmin" released from hepatic dead cells in response to tissue damages, the interrelationship between IL-33-mediated hepatocyte autophagy and innate immune response in the acetaminophen (APAP)-induced liver injury (AILI) process remains obscure. This study aimed to explore the regulation of IL-33 on hepatocyte autophagy and macrophage polarization after APAP challenge in vivo and vitro. We found IL-33 released from hepatic necrosis was elevated in the AILI mouse model. Blockage of IL-33 exacerbated liver injury by consuming liver-resident macrophages cells (Kupffer cells, KCs) and promoting hepatic inflammatory factors secretion, such as TNF-α, IL-6 and IL-1ß in the early phase of liver injury. Interestingly, IL-33 deficiency further activated hepatocyte autophagy and disrupted M2 macrophage polarization post-APAP challenge in vivo and vitro, which can be reversed by recombinant IL-33 treatment. Mechanistically, administration of IL-33 can directly enhance M2 polarization via PI3K/Akt signaling pathway and activate protective hepatocyte autophagy via AMPKα/mTOR signaling pathway in the AILI process. In conclusion, our data firstly demonstrates that IL-33 exerts protective effects on hepatocytes through the activation of autophagy and functions as an innate immunity regulator mediating macrophage polarization in the early phase of AILI.

Spatial distribution of heavy metal contamination and uncertainty-based human health risk in the aquatic environment using multivariate statistical method.

Heavy metal contamination in the aquatic environment is one of the most serious health issues worldwide. In this study, an evaluation framework is developed to identify the sources and health risk of heavy metals (i.e., As, Hg, Cr, Cu, Zn, Pb, and Cd) contamination in the North Canal of Fengtai District, China, which is based on multiple approaches, including multivariate statistical method, health risk assessment, and uncertainty analysis. Spatial distribution of these heavy metals could exhibit their impact on the aquatic environment. Pearson's correlation analysis shows that a majority of the correlations between different heavy metals are not significant due to the differences in sources of heavy metals. Principal component analysis indicates that there are four principal components to explain 91.381% of the total variance. Moreover, health risk reveals that hazard quotient values are in low levels, ranging from 0.48 to 0.74, relative higher quotient levels could be observed in the northern section. The carcinogenic risk of Cd has exceeded the acceptable level in S1, S3, and S7. Sensitivity analysis ensures the reliability of health risk assessments. Furthermore, some specific recommendations are given to help decision-makers develop more comprehensive strategies for improving water environment quality.

Multi-criteria decision making and fairness evaluation of water ecological carrying capacity for inter-regional green development.

Determining water ecological carrying capacity (WECC) is of great significance to ensure inter-regional green development. This study presents a comprehensive evaluation framework for WECC assessment in the Yangtze River Economic Zone (YREZ), China. Effects of water resources, socio-economic, and ecological elements on WECC can be evaluated based on multi-criteria decision analysis. Gini and unbalance coefficients are used for measuring the regional fairness between WECC and socio-economic development. Surface water production pressure (SWPP) and groundwater pollution risk (GPR) are further regarded as indicators for expressing water resources constraint on shale gas extraction in the YREZ. Results disclose that the average WECC level decreases from 0.439 in 2000 to 0.4007 in 2016, which is the opposite of the changing trend in the Beijing-Tianjin-Hebei Region. A high WECC level appears in Zhejiang (0.5126) with a good state, but that of Guizhou (0.3983), Anhui (0.3968), Hunan (0.3914), and Chongqing (0.3651) are at the alert state. The obstacle factors of WECC in the eastern YREZ mostly originate from socio-economic and water resource subsystems, while that in the middle and western YREZ mainly arise from water resources and ecological subsystems. Fairness analysis shows a well-matching characteristic between the overall WECC and socio-economic performances due to a majority of their Gini coefficients lower than 0.4, while a poor matching characteristic exists in terms of provincial differences owing to their varied unbalance coefficients, especially in Guizhou, Jiangsu, and Shanghai. Moreover, Chongqing with most of shale gas reserves is characterized by slight SWPP (1.0202) and GPR (0.0188), but the prospect of shale gas development in Sichuan is not optimistic due to its high SWPP (1.0846) and GPR (0.0647). Recycling of flowback and product waters can significantly lighten regional water resources pressure. This presented framework can be applied into many other Chinese cities (e.g., Beijing-Tianjin-Hebei Region) with slight modifications according to their actual situations for supporting water resource managers and government with decision making.

Increased Choroidal Blood Perfusion Can Inhibit Form Deprivation Myopia in Guinea Pigs.

Purpose: In guinea pigs, choroidal thickness (ChT) and choroidal blood perfusion (ChBP) simultaneously decrease in experimental myopia, and both increase during recovery. However, the causal relationship between ChBP and myopia requires further investigation. In this study, we examined the changes of ChBP with three different antimyopia treatments. We also actively increased ChBP to examine the direct effect on myopia development in guinea pigs. Methods: Experiment 1: Guinea pigs wore occluders on the right eye for two weeks to induce form-deprivation myopia (FDM). Simultaneously they received daily antimyopia treatments: peribulbar injections of atropine or apomorphine or exposure to intense light. Experiment 2: The vasodilator prazosin was injected daily into the form-deprivation eyes to increase ChBP during the two-week induction of FDM. Other FDM animals received appropriate control treatments. Changes in refraction, axial length, ChBP, ChT, and hypoxia-labeled pimonidazole adducts in the sclera were measured. Results: The antimyopia treatments atropine, apomorphine, and intense light all significantly inhibited myopia development and the decrease in ChBP. The treatments also reduced scleral hypoxia, as indicated by the decrease in hypoxic signals. Furthermore, actively increasing ChBP with prazosin inhibited the progression of myopia, as well as the increase in axial length and scleral hypoxia. Conclusions: Our data strongly indicate that increased ChBP attenuates scleral hypoxia, and thereby inhibits the development of myopia. Thus ChBP may be a promising target for myopia retardation. As such, it can serve as an immediate predictor of myopia development as well as a long-term marker of it.

Autophagy mediates bronchial cell malignant transformation induced by chronic arsenic exposure via MEK/ERK1/2 pathway.

Chronic exposure to arsenic increases the risk of developing a variety of human cancers including lung carcinomas. However, the exact molecular mechanism underlying arsenic carcinogenicity remains largely unknown. Autophagy is a conserved catabolic process for maintaining cellular protein homeostasis whose defects might result in accumulation of dysfunctional organelles and damaged proteins thus promoting tumorigenesis. In the present study, we found that chronic exposure of human bronchial epithelial BEAS-2B cells to sub-lethal dose of sodium arsenite led to autophagy activation and induced an epithelial-to-mesenchymal transition (EMT) to enhance cell migratory and invasive capability. The malignant transformation was mediated via activation of MEK/ERK1/2 signaling. Importantly, inhibition of autophagy in these arsenic-exposed cells by pharmacological intervention or genetic deletion further promoted the EMT and increased the generation of inflammasomes. Both autophagy inhibitor and genetic deletion of autophagy core gene Beclin-1 produced similar effects. These results may suggest the important role of autophagy in sodium arsenite-induced lung tumorigenesis which may serve as a potential target in prevention and treatment of arsenic-imposed lung cancer.