Effect of colloidal magnetite (Fe3O4) nanoparticles on the electrical characteristics of the azolectin bilayer in a static inhomogeneous magnetic field.

This work is devoted to the study of the combined effects of applied magnetic field and MNPs on the electrical characteristics of bilayer lipid membranes. We present results of the study of electrical parameters of azolectin membranes in a static inhomogeneous magnetic field at the one-sided addition of positively charged quasi-spherical superparamagnetic magnetite nanoparticles with a diameter of about 4 nm. The magnet was located at different distances from the membrane, and the magnetic field attracted the nanoparticles to the membrane surface with different strengths. We observed three pronounced effects that depended on the external magnetic field. Firstly, after addition of nanoparticles in a magnetic field, the conductance of the membranes increased. A smooth increase in conductance was accompanied in some cases by the appearance of current jumps, which can be associated with the formation of through pores with a radius of no more than 1 nm. The conductance increased with increasing magnetic field gradient. Secondly, at zero command voltage, a negative current through the membrane was observed. Although our experiments did not allow us to unambiguously determine which particles create this current, we believe that this current is associated with the penetration of particles through the membrane. This effect intensified with increasing magnetic field gradient. Thirdly, we observed a sharp change in the nonlinear dependence of capacitance on voltage associated both with the change in the surface potential of the azolectin membrane and with the effect of MNP binding to the membrane surface on the apparent membrane capacitance.

Bioelectric impedancemetry of the myocardium during cardiac surgery with artificial circulation.

We analyzed changes in bioelectric impedance of the myocardium during cardiac surgery under conditions of cardioplegy. The results suggest the possibility of using bioelectric impedancemetry for evaluation of the state of the myocardium during surgery. Bioelectric impedancemetry allows evaluation of interstitium filling during infusion of the cardioplegic solution and reperfusion damage to cardiomyocytes.

Preliminary static EIT images of the thorax in health and disease.

The results of a preliminary clinical evaluation of a one-frequency electrical impedance tomography (EIT) system enabling static in vivo imaging are presented. The design of the measuring system and image reconstruction software are described. Thirty-one subjects were examined and divided into four clinical groups. The first group consisted of 22 patients with clinical diagnosis of lung cancer with tumour localization in one lung. The second group consisted of seven healthy subjects. A patient after a one-sided pneumectomy and another with one-sided emphysema diagnosis were also examined. Static EIT images of a healthy human chest and a chest with various abnormalities are given and discussed. The evaluated system distinguishably visualizes various states of lungs and thorax including lung cancer. The average static conductivity of an affected lung in the first clinical group statistically differs from the average conductivity of a healthy lung. In spite of low spatial resolution, according to preliminary results, the method can be sensitive to cancer and other lung diseases in screening investigations.

A 3D electrical impedance tomography (EIT) system for breast cancer detection.

A medical device which allows imaging of the distribution of conductivity in 3D in regions below the skin surface has been developed and tested. Its purpose is to enable early detection and preliminary diagnosis of breast tumours. Design of the measuring system and software are described. Results of clinical evaluation of the system are presented. EIT images of healthy and cancerous breasts are presented and discussed. The system is able to visualize various states of the breast and it may be possible to apply it to breast cancer detection.

Magnetic induction tomography: experimental realization.

Magnetic induction tomography (MIT) is a new non-contacting technique for visualization of the electrical impedance distribution inside inhomogeneous media. A measuring system for MIT has been developed. An oscillating magnetic field is applied in the system as a sounding agent. The system is designed mainly for biomedical applications. Experiments demonstrate that with proper selection of measurement conditions it is possible to use the phase shifts between inductor and detector signals for image reconstruction by filtered backprojection along magnetic lines. Measurements with saline filled phantoms having various spatial distributions of conductivity were carried out and images were reconstructed. The experiments have demonstrated the applicability of MIT for medical imaging and diagnostics.