Assuntos
Procedimentos Cirúrgicos Ambulatórios , Histeroscopia , Gravidez , Feminino , Humanos , Consenso , População do Leste AsiáticoRESUMO
OBJECTIVE: Ovarian cancer is one of the most common causes of cancer-related deaths in women. Many studies show that dysregulated gene expression plays a key role in tumorigenesis and development. Therefore, a comprehensive understanding of ovarian serous cystadenocarcinoma survival prognosis is needed. PATIENTS AND METHODS: A large number of high-dimensional RNA-sequencing files and clinical datasets collected from the Genomic Data Commons Data Portal were utilized to identify novel potential biomarkers for determining the prognosis of patients with ovarian serous cystadenocarcinoma (OVSC). We adopted a new strategy to identify these biomarkers by integrating co-expression network analysis and the Kaplan-Meier estimation with a non-parametric bootstrapping procedure. RESULTS: Functional enrichment analysis of gene modules of interest revealed several dysregulated genes in OVSC, suggesting a close relationship between hormones and angiogenesis. In combination with this comprehensive approach, 14 genes, including ABCA10, DCX, LRRC30, ALX4, DKK4, SGCZ, ANKS4B, FHL5, SPRR2F, CHRNG, GABRR1, STMN2, CRHBP, and GSTM5, were shown to serve as candidate biomarkers for predicting the prognosis of patients with OVSC. CONCLUSIONS: The current study identified several valuable prognostic biomarkers and several potential therapeutic targets for treating OVSC.
Assuntos
Cistadenocarcinoma Seroso/diagnóstico , Neoplasias Ovarianas/diagnóstico , Biomarcadores/análise , Cistadenocarcinoma Seroso/química , Cistadenocarcinoma Seroso/genética , Cistadenocarcinoma Seroso/mortalidade , Feminino , Regulação Neoplásica da Expressão Gênica , Redes Reguladoras de Genes/genética , Genes Neoplásicos/genética , Marcadores Genéticos/genética , Humanos , Estimativa de Kaplan-Meier , Neoplasias Ovarianas/química , Neoplasias Ovarianas/genética , Neoplasias Ovarianas/mortalidade , Ovário/química , Prognóstico , Análise de Sobrevida , TranscriptomaRESUMO
OBJECTIVE: To investigate the effect of Deltex-1 on proliferation and differentiation of bone marrow mesenchymal stem cells (bMSCs) into smooth muscle cells (SMCs). MATERIALS AND METHODS: bMSCs of rat were isolated from bone marrow, cultured and identified. The effect of Deltex-1 on the proliferation of bMSCs infected with adenovirus vector pAd/Deltex-1 was detected by cell count kit-8 (CCK-8). The expression of smooth muscle myosin heavy chain (SM-MHC), that is one of the markers of SMCs in bMSCs without treatment, with Deltex-1 virus infection or empty virus infection and co-cultured with SMCs, were detected by immunofluorescence cytochemistry staining, RT-PCR and Western blotting. The same detection of bMSCs and SMCs without treatment was used as normal control, respectively. RESULTS: bMSCs of rat were isolated from bone marrow, cultured and identified. Compared with the control, the results of CCK-8 showed that the growth of bMSCs infected by Deltex-1 virus was slower, and began to appear more significant especially at 48 (p<0.05, p<0.01). The results of immunofluorescence cytochemistry, Real-time PCR and Western blot showed that bMSCs with Deltex-1 virus infection and co-cultured with SMCs significantly expressed SM-MHC, and weakly expressed Notch-1. CONCLUSIONS: The proliferation of bMSCs with Deltex-1 over-expression could be inhibited and its differentiation into smooth muscle cells could be promoted.
Assuntos
Células da Medula Óssea , Células-Tronco Mesenquimais/efeitos dos fármacos , Miócitos de Músculo Liso/efeitos dos fármacos , Ubiquitina-Proteína Ligases , Animais , Diferenciação Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Células Cultivadas , Dependovirus/genética , Miócitos de Músculo Liso/citologia , Cadeias Pesadas de Miosina/metabolismo , Ratos , Ratos Sprague-Dawley , Sincalida/metabolismoRESUMO
Crop impaction and candidiasis was encountered in young, meat-type pigeons (hybrid line). Prominent lesions were epithelial hyperplasia, severe hydropic degeneration and microvesicular formation in the crop. There was tissue invasion by candida blastospores and pseudohyphae. The cause was attributed to hand-feeding too much unsuitable feed at too early an age. Similar lesions were induced in an experimental study.